4,111 research outputs found
MicroRNA activity in the Arabidopsis male germline
Most of the core proteins involved in the microRNA (miRNA) pathway in plants have been identified, and almost simultaneously hundreds of miRNA sequences processed in the Arabidopsis sporophyte have been discovered by exploiting next-generation sequencing technologies. However, there is very limited understanding about potentially distinct mechanisms of post-transcriptional regulation between different cell lineages. In this review the focus is on the Arabidopsis male gametophyte (pollen), where the germline differentiates after meiosis giving rise to the male gametes. Based on comparative analysis of miRNAs identified in sperm cells by in-depth sequencing, their possible functions during germ cell specification and beyond fertilization are discussed. In addition, 25 potentially novel miRNAs processed in sperm cells and pollen were identified, as well as enriched variations in the sequence length of known miRNAs, which might indicate subfunctionalization by association with a putative germline-specific Argonaute complex. ARGONAUTE 5 (AGO5), by close homology to AGO1 and localizing preferentially to the sperm cell cytoplasm in mature pollen, may be part of such a complex
Radial abundance gradients in the outer Galactic disk as traced by main-sequence OB stars
Using a sample of 31 main-sequence OB stars located between galactocentric
distances 8.4 - 15.6 kpc, we aim to probe the present-day radial abundance
gradients of the Galactic disk. The analysis is based on high-resolution
spectra obtained with the MIKE spectrograph on the Magellan Clay 6.5-m
telescope on Las Campanas. We used a non-NLTE analysis in a self-consistent
semi-automatic routine based on TLUSTY and SYNSPEC to determine atmospheric
parameters and chemical abundances. Stellar parameters (effective temperature,
surface gravity, projected rotational velocity, microturbulence, and
macroturbulence) and silicon and oxygen abundances are presented for 28 stars
located beyond 9 kpc from the Galactic centre plus three stars in the solar
neighborhood. The stars of our sample are mostly on the main-sequence, with
effective temperatures between 20800 - 31300 K, and surface gravities between
3.23 - 4.45 dex. The radial oxygen and silicon abundance gradients are negative
and have slopes of -0.07 dex/kpc and -0.09 dex/kpc, respectively, in the region
\,kpc. The obtained gradients are compatible with the
present-day oxygen and silicon abundances measured in the solar neighborhood
and are consistent with radial metallicity gradients predicted by
chemodynamical models of Galaxy Evolution for a subsample of young stars
located close to the Galactic plane.Comment: Accepted for publication in the A&
Open and hidden charm in proton-nucleus and heavy-ion collisions
We review the collectivity and the suppression pattern of charmed mesons -
produced in proton-nucleus and nucleus-nucleus collisions at SPS (158 AGeV) and
RHIC energies (21 ATeV) - in comparison to dynamical and thermal models. In
particular, we examine the charmonium `melting' and the `comover dissociation'
scenarios - implemented in a microscopic transport approach - in comparison to
the available data from the SPS and RHIC. The analysis shows that the dynamics
of c, c-bar quarks at RHIC are dominated by partonic or `pre-hadronic'
interactions in the strongly coupled plasma stage. Both the `charmonium
melting' and the hadronic `comover absorption and recreation model' are found,
however, to be compatible with the experimental observation at SPS energies;
the experimental ratio of Psi'/J/Psi versus centrality clearly favors the
`hadronic comover' scenario. We find that the collective flow of charm in the
purely hadronic Hadron-String Dynamics (HSD) transport appears compatible with
the data at SPS energies, but substantially underestimates the data at top RHIC
energies. Thus, the large elliptic flow v2 of D-mesons and the low R_AA(p_T) of
J/Psi seen experimentally have to be attributed to early interactions of
non-hadronic degrees of freedom. Simultaneously, we observe that non-hadronic
interactions are mandatory in order to describe the narrowing of the J/Psi
rapidity distribution from pp to central Au+Au collisions at the top RHIC
energy. We demonstrate additionally that the strong quenching of low-pT J/Psi's
in central Au+Au collisions indicates that a large fraction of final J/Psi
mesons is created by a coalescence mechanism close to the phase boundary.
Throughout this review we, furthermore, provide predictions for charm
observables from Au+Au collisions at FAIR energies of 25-35 AGeV.Comment: review for Int. J. Mod. Phys. E, 75 pages, 50 figure
Morphology of supported polymer electrolyte ultra-thin films: a numerical study
Morphology of polymer electrolytes membranes (PEM), e.g., Nafion, inside PEM
fuel cell catalyst layers has significant impact on the electrochemical
activity and transport phenomena that determine cell performance. In those
regions, Nafion can be found as an ultra-thin film, coating the catalyst and
the catalyst support surfaces. The impact of the hydrophilic/hydrophobic
character of these surfaces on the structural formation of the films has not
been sufficiently explored yet. Here, we report about Molecular Dynamics
simulation investigation of the substrate effects on the ionomer ultra-thin
film morphology at different hydration levels. We use a mean-field-like model
we introduced in previous publications for the interaction of the hydrated
Nafion ionomer with a substrate, characterized by a tunable degree of
hydrophilicity. We show that the affinity of the substrate with water plays a
crucial role in the molecular rearrangement of the ionomer film, resulting in
completely different morphologies. Detailed structural description in different
regions of the film shows evidences of strongly heterogeneous behavior. A
qualitative discussion of the implications of our observations on the PEMFC
catalyst layer performance is finally proposed
Measurements and analysis of the upper critical field on an underdoped and overdoped compounds
The upper critical field is one of the many non conventional
properties of high- cuprates. It is possible that the
anomalies are due to the presence of inhomogeneities in the local charge
carrier density of the planes. In order to study this point, we
have prepared good quality samples of polycrystalline
using the wet-chemical method, which has demonstrated to produce samples with a
better cation distribution. In particular, we have studied the temperature
dependence of the second critical field, , through the magnetization
measurements on two samples with opposite average carrier concentration
() and nearly the same critical temperature, namely
(underdoped) and (overdoped). The results close to do not
follow the usual Ginzburg-Landau theory and are interpreted by a theory which
takes into account the influence of the inhomogeneities.Comment: Published versio
Tipitamba Project: fire-free land preparation as an alternative to slash-and-burn agriculture in Amazon, Brazil.
Steady states in a structured epidemic model with Wentzell boundary condition
We introduce a nonlinear structured population model with diffusion in the
state space. Individuals are structured with respect to a continuous variable
which represents a pathogen load. The class of uninfected individuals
constitutes a special compartment that carries mass, hence the model is
equipped with generalized Wentzell (or dynamic) boundary conditions. Our model
is intended to describe the spread of infection of a vertically transmitted
disease, for example Wolbachia in a mosquito population. Therefore the
(infinite dimensional) nonlinearity arises in the recruitment term. First we
establish global existence of solutions and the Principle of Linearised
Stability for our model. Then, in our main result, we formulate simple
conditions, which guarantee the existence of non-trivial steady states of the
model. Our method utilizes an operator theoretic framework combined with a
fixed point approach. Finally, in the last section we establish a sufficient
condition for the local asymptotic stability of the positive steady state
Identification of oligomerization and drug-binding domains of the membrane fusion protein EmrA
Many pathogenic Gram-negative bacteria possess tripartite transporters that catalyze drug extrusion across the inner and outer membranes, thereby conferring resistance. These transporters consist of inner (IMP) and outer (OMP) membrane proteins, which are coupled by a periplasmic membrane fusion (MFP) protein. However, it is not know whether the MFP translocates the drug between the membranes, by acting as a channel, or whether it brings the IMP and OMP together, facilitating drug transfer. The MFP EmrA has an elongated periplasmic domain, which binds transported drugs, and is anchored to the inner membrane by a single -helix, which contains a leucine zipper dimerization domain. Consistent with CD and hydrodynamic analyses, the periplasmic domain is predicted to be composed of a -sheet subdomain and an -helical coiled-coil. We propose that EmrA forms a trimer in which the coiled-coils radiate across the periplasm, where they could sequester the OMP TolC. The "free" leucine zipper in the EmrA trimer might stabilize the interaction with the IMP EmrB, which also possesses leucine zipper motifs in the putative N- and C-terminal helices. The -sheet subdomain of EmrA would sit at the membrane surface adjacent to the EmrB, from which it receives the transported drug, inducing a conformational change that triggers the interaction with the OMP
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