POLY(GLYCIDYL METHACRYLATE-DIVINYLBENZENE) MONOLITHIC CAPILLARY AS A STATIONARY PHASE FOR THE REVERSED-PHASE CHROMATOGRAPHIC SEPARATION OF PROTEINS

Capillary column with monolithic stationary phase was prepared from silanized fused-silica capillary of 200 µm I.D. by in situ free radical polymerization of divinylbenzene with glycidy methacrylate in the presence of decanol and tetrahydrofuran as porogens.  The hydrodynamic and chromatographic properties of this monolith, such as backpressure at different flow-rate, pore size distribution, van Deemter plot and the effect of varying gradient-rate were investigated.  Poly(glycidyl methacrylate-divinylbenzene) monolithic capillary has been used successfully for the reversed-phase chromatographic separation of proteins.   Keywords: monolithic stationary phase, poly(glycidyl methacrylate-divinylbenzene), protein

New particle formation in the Front Range of the Colorado Rocky Mountains

New particle formation is of interest because of its influence on the properties of aerosol population, and due to the possible contribution of newly formed particles to cloud condensation nuclei. Currently no conclusive evidence exists as to the mechanism or mechanisms of nucleation and subsequent particle growth. However, nucleation rates exhibit a clear dependence on ambient sulphuric acid concentrations and particle growth is often attributed to the condensation of organic vapours. A detailed study of new particle formation in the Front Range of the Colorado Rocky Mountains is presented here. Gas and particle measurement data for 32 days was analyzed to identify event days, possible event days, and non-event days. A detailed analysis of nucleation and growth is provided for four days on which new particle formation was clearly observed. Evidence for the role of sesquiterpenes in new particle formation is presented

Boronic acid–lectin affinity chromatography. 1. Simultaneous glycoprotein binding with selective or combined elution

We introduce a novel combination of boronic acid affinity chromatography with lectin affinity chromatography, dubbed as boronic acid–lectin affinity chromatography (BLAC). Concanavalin A and wheat germ agglutinin lectins were mixed with the pesudo-lectin boronic acid to form the BLAC affinity column and their performance was evaluated with standard glycoproteins. Optimization of the binding and elution buffers for the BLAC system is described. The BLAC columns were employed to isolate glycoproteins of interest using both selective and/or combined elution

Sample Preparation for the Analysis of Complex Carbohydrates by Multicapillary Gel Electrophoresis with Light-Emitting Diode Induced Fluorescence Detection

This paper evaluates various sample preparation methods for multicapillary gel electrophoresis based glycan analysis to support electrokinetic injection. First the removal of excess derivatization reagent is discussed. Although the Sephadex G10 filled multiscreen 96-well filter plate and Sephadex G10 filled pipet tips enabled increased analysis sensitivity, polyamide DPA-6S pipet tips worked particularly well. In this latter case an automated liquid handling system was used to increase purification throughput, necessary to feed the multicapillary electrophoresis unit. Problems associated with the high glucose content of such biological samples as normal human plasma were solved by applying ultrafiltration. Finally, a volatile buffer system was developed for exoglycosidase-based carbohydrate analysis

MONOLITHIC DISK FOR THE FAST CHROMATOGRAPHIC SEPARATION

Poly(styrene/divinylbenzene) (PS/DVB) monolithic disk was prepared by in situ free-radical copolymerization of styrene and divinylbenzene in the presence of decanol and tetrahydrofuran as porogens. PS/DVB monolithic disks were produced in two different lengths 1.5 mm and 3 mm. The disks were used in reversed phase chromatography of proteins with 0.2 % trifuoroacetic acid (TFA) and 0.2 % TFA in acetonitrile as mobile phase A and B, respectively. The effect of gradient rate, flow rate, temperature and disk length on the separation of proteins were also studied. PS/DVB monolithic disks allow the rapid separation of proteins in reversed phase chromatography. Keywords: monolithic disk, poly(styrene/divinylbenzene), protein

Einfluss von Verkehrserschuetterungen waehrend des Anbringens grossflaechiger Bauteilergaenzungen auf deren Dauerhaftigkeit bei praxisnahen Beanspruchungen

Within the framework of this research project the possibility of applying large-surface building component supplements under pulsating loading was studied. In addition to the behaviour of various supplementary materials (concrete with and without polymer dispersion and shotcrete) the influence of bonding layers, dowels and fabric reinforcement in the supplementary material could be studied as well as the influence of secondary treatment measures on the success of the repair work. To test the durability of the repair measures undertaken, the five beams supplemented on both sides and the two beams supplemented from below were first subjected to static and dynamic loads and then exposed to a one-year permanent load under natural weathering until they were loaded to the defined breaking load. By direct comparison of the load-bearing, cracking and deformation behaviour with beams supplemented in an unstressed state, the influence of the pulsating load during the application of the building component supplement on the success of the repair work could be established. The best results concerning the durability were displayed by the beams supplemented with normal concrete in formwork. This concerned above all the test specimens which were provided with dowels and fabric reinforcement in the supplementary layer and were given a corresponding secondary treatment. Shotcreting work is able to achieve similarly good results by using pre-fabricated materials. With the materials supplemented with PCC, the largest differences between the girders supplemented in an unstressed state and the girders supplemented under pulsating load were observed. However, the comparisons of the repair methods themselves also show that for large-surface building component supplements PCCs are not to be recommended. Adding bonding layers contains risks for all types of supplements; clear increases in the adhesive strength in the bond joint are not be be expected with thick-layered supplements by means of bonding layers. (orig.)Available from TIB Hannover: ZA 4681(644)+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Au-Nanomaterials as a Superior Choice for Near-Infrared Photothermal Therapy

Photothermal therapy (PPT) is a platform to fight cancer by using multiplexed interactive plasmonic nanomaterials as probes in combination with the excellent therapeutic performance of near-infrared (NIR) light. With recent rapid developments in optics and nanotechnology, plasmonic materials have potential in cancer diagnosis and treatment, but there are some concerns regarding their clinical use. The primary concerns include the design of plasmonic nanomaterials which are taken up by the tissues, perform their function and then clear out from the body. Gold nanoparticles (Au NPs) can be developed in different morphologies and functionalized to assist the photothermal therapy in a way that they have clinical value. This review outlines the diverse Au morphologies, their distinctive characteristics, concerns and limitations to provide an idea of the requirements in the field of NIR-based therapeutics

Auswirkungen wasserwirtschaftlicher Massnahmen auf die Umwelt sowie Moeglichkeiten ihrer Erfassung und Bewertung

TIB: AC 6783 (Anh 2) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman