234 research outputs found
Geologic map, volcanic stratigraphy and structure of the Cabo de Gata volcanic zone, Betic-Rif orogen, SE Spain
The geologic map of the Neogene Cabo de Gata volcanic zone is presented together with a comprehensive volcanic stratigraphy and structure based on logging, correlation and mapping. Volcanic rocks are interbedded with sedimentary rocks throughout the Cabo de Gata volcanic zone. The volcano-sedimentary succession of Cabo de Gata has been divided into formations according to lithology, age, composition and stratigraphic position. The contacts between sedimentary units and volcanic units and between formations are unconformities. Sedimentary units were deposited during periods of volcanic repose. The depositional environment of volcanism in Cabo de Gata is characterized as shallow-water submarine to emergent based on lithofacies of volcanic rocks and on fossil content and sedimentary structures of sedimentary rocks. The eruptive style in Cabo de Gata is dominantly effusive, although small-volume explosive eruptions due to magma-water interaction processes and to explosions of lava flow and domes complexes occurred.Peer Reviewe
SUSCEPTIBILITY TO MAIN ANTIBIOTICS OF GRAM-NEGATIVE BACTERIA FROM BOVINE MASTITIS IN ITALIAN HERDS
Introduction .The incorrect use of antibiotics in veterinary medicine has contributed to reduce the pathogen susceptibility to antibiotics. Through direct contact or food chain the resistant bacteria can be transferred to humans (2). In dairy cows, differently from other production animals, milk discharge during antibiotic administration and related withdrawal time has a direct economical effect on production. This issue has determined a more responsible use of antibiotics. Moreover, in mastitis field antibiotics are used mainly to treat clinical cases and not for prophylaxis, except when blanket dry-cow therapy is applied. For this reason monitoring susceptibility to antibiotic-resistance in dairy cows could identify different trend respect to other food animals.
Gram-negative bacteria are frequently cause of clinical mastitis and, as part of enteric flora, they are subjected to the selective pressure of systemic drug treatments. For this reason they are subjected to specific monitoring program for antibiotic resistance. This paper describes a study about in vitro antimicrobial susceptibility of Gram negative bacteria isolated from bovine clinical mastitis in Northern Italy.
Materials and Methods
Among Gram negative bacteria isolated from bovine clinical mastitis in Northern Italy during the years 2010/2011, 99 isolates were selected: 53 E.coli, 28 Klebsiella spp, 14 Serratia spp, 2 Enterobacter spp. e 2 Citrobacter spp. Pathogens isolation was carried out in milk samples following the National Mastitis Council guideline (3). Species identification was performed through commercial kits API 20 and API 20 NE (Biomerieux Marcy l\u2019Etoile, France). In vitro antimicrobial susceptibility was evaluated using minimal inhibitory concentrations (MICs) broth dilution test, according to the procedure described in Clinical and Laboratory Standards Institute (CLSI) guideline. CLSI resistance breakpoints were used (1). Sensititre susceptibility plates were used according to supplier instructions (Trek Diagnostics System, East Grinstead, UK). The antibiotics used are listed in table 1. After incubation the plates were manually read recording the last dilution able to inhibit bacterial growth.
Results and Discussion
The 36% of tested E. coli resulted resistant towards ampicillin, while using penicillins associated to clavulanic acid the percentage of resistant isolates declined to 7,5%. Serratia spp isolates demonstrated a higher resistance level to amoxicillin/clavulanic acid, with susceptibility reduced to 74%. Cephalosporins confirmed a very high efficacy in the regards of E coli, as 94,3% of isolates were susceptible to 1st generation cephalosporins and 100% to 3rd generation (ceftiofur and cefpodoxime). Similar results were obtained for Klebsiella spp, while all Serratia spp tested isolates revealed some resistance to 1st generation cephalosporins, maintaining susceptibility to 3rd generation cephalosporins. Among drugs active specifically towards Gram-negatives bacteria, chloramphenicol and sulfonamide associated with trimethoprim demonstrated high efficacy to all tested species. Average susceptibility was 85% for sulfonamide with trimethoprim, and 95% for chloramphenicol. Finally, only one E. coli isolate resulted resistant to fluoroquinolons with an intermediate MIC for marbofloxacin and enrofloxacin, while all the others 98 Gram-negative isolates tested were susceptible (MIC < 0,5 \uf06dg/mL). Complete data are shown in table 1.
Table 1. In vitro susceptibility and resistance of E. coli and Klebsiella spp., Serratia spp., Enterobacter spp. and Citrobacter spp. (joined in \u201cother Gram-negatives\u201d) towards tested antibiotics.
Antibiotics Sensible E. coli (%) Resistant E. coli (%) Sensible other Gram-negatives (%) Resistant other Gram-negatives (%)
Ampicillin 64,2% 35,8% 30,4% 69,6%
Amoxicillin/clavulanic acid 92,5% 7,5% 73,9% 26,1%
Amikacin 100,0% 0,0% 100,0% 0,0%
Cefpodoxime (3rd gen. Ceph.) 100,0% 0,0% 100,0% 0,0%
Ticarcillin/clavulanic acid 94,3% 5,7% 89,1% 10,9%
Sulfonamide/thrimethoprim 86,8% 13,2% 89,1% 10,9%
Cefazolin (1\ub0st gen. Ceph.) 94,3% 5,7% 63,0% 37,0%
Gentamicin 100,0% 0,0% 100,0% 0,0%
Imipenem 100,0% 0,0% 100,0% 0,0%
Penicillin 0,0% 100,0% 0,0% 100,0%
Doxiciclin 75,5% 24,5% 71,7% 28,3%
Ceftiofur (3rd gen. Ceph.) 100,0% 0,0% 100,0% 0,0%
Marbofloxacin 98,1% 1,9% 100,0% 0,0%
Enrofloxacin 98,1% 1,9% 100,0% 0,0%
Chloramphenicol 94,3% 5,7% 97,8% 2,2%
In conclusion, this study demonstrates that Gram-negative bacteria commonly isolable in case of mastitis are susceptible to most antibiotics, included fluoroquinolons, sulfonamide and chloramphenicol. Reduced susceptibility to some beta-lactams in E.coli are avoided associating clavulanic acid, an inhibitor of beta-lactamases. Serratia spp. is an exception probably due to specific beta-lactamases produced, which are resistant to inhibitors. This findings confirm the importance of milk culture in mastitis cases to achieve an etiological diagnosis, that allows to choose treatment or culling for chronically infected animals. The aims of treatment procedures in farm animal should be avoiding abuse of antibiotic therapy and reducing selection of resistant strains. Periodical survey and detection of resistance phenomenon are recommended.
References
1. CLSI. (2008). Performances standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals: approved standard \u2013 third edition CLSI document M31-A3. CLSI Wayne, PA, (USA): Clinical and Laboratory Standard Institute.
2. Friedman D.B., Kanwat CP, Headrick ML, Patterson NJ, Neely JC, Smith LU. (2007) Importance of prudent antibiotic use on dairy farms in south carolina: a pilot project on farmers\u2019 knowledge, attitudes and practices. Zoonoses Public Health. 54, 366\u2013375.
3.NATIONAL MASTITIS COUNCIL (1999). Laboratory and field handbook on bovine mastitis. National Mastitis Council, Madison WI
Clinical and Immunologic Features of Ultra-short Celiac Disease
BACKGROUND & AIMS: The clinical effects of gluten-sensitive
enteropathy with villous atrophy limited to the duodenal bulb
(D1) have not been delineated in adults with celiac disease. We
investigated the sensitivity of D1 biopsy analysis in the detection
of celiac disease, the number and sites of biopsies required
to detect ultra-short celiac disease (USCD, villous atrophy
limited to D1), and the clinical phenotype of USCD. METHODS:
We performed a prospective study of 1378 patients (mean age,
50.3 y; 62% female) who underwent endoscopy at a tertiary
medical center in the United Kingdom from 2008 through
2014; routine duodenal biopsy specimens were collected from
D1 and the second part of the duodenum (D2). Quadrantic D1
biopsy specimens were collected from 171 consecutive patients
with a high suspicion of celiac disease (mean age, 46.5 y; 64%
female). Clinical data from patients diagnosed with USCD, based
on biopsy analysis, were compared with those from patients
with conventional celiac disease (CCD) (villous atrophy beyond
D1) and individuals without celiac disease (controls). The
number of intraepithelial lymphocytes (IELs) and immune
phenotypes were compared between D1 vs D2 in patients with
celiac disease. RESULTS: Of the 1378 patients assessed, 268
(19.4%) were diagnosed with celiac disease; 9.7% of these
patients had villous atrophy confined to D1 (USCD; P < .0001).
Collection of a single additional biopsy specimen from any D1
site increased the sensitivity of celiac disease detection by
9.3%–10.8% (P < .0001). Patients with USCD were younger
(P ¼ .03), had lower titers of tissue transglutaminase antibody
(P ¼ .001), and less frequently presented with diarrhea
(P ¼ .001) than patients with CCD. Higher proportions of
patients with CCD had ferritin deficiency (P ¼ .007) or folate
deficiency (P ¼ .003) than patients with USCD or controls.
Patients with celiac disease had a median of 50 IELs/100
enterocytes in D1 and a median of 48 IELs/100 enterocytes
(P ¼ .7) in D2. The phenotype of IELs from patients with D1
celiac disease was indistinguishable from those of patients with
D2 celiac disease. CONCLUSIONS: Collection of a single
additional biopsy specimen from any site in the D1 intestine
increases the sensitivity of detection for celiac disease. Patients
with USCD may have early stage or limited celiac disease, with a
mild clinical phenotype and infrequent nutritional deficiencies
Diagnosing celiac disease by video capsule endoscopy (VCE) when esophogastroduodenoscopy (EGD) and biopsy is unable to provide a diagnosis: a case series
Three-dimensional reconstruction of the main unconformities of the Colli Albani stratigraphy and deposit volume calculations
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