Streaming flow by oscillating bubbles: Quantitative diagnostics via particle tracking velocimetry

Oscillating microbubbles can be used as microscopic agents. Using external acoustic fields they are able to set the surrounding fluid into motion, Erode surfaces and even to carry particles attached to their interfaces. Although the acoustic streaming flow that the bubble generates in its vicinity has been often observed, it has never been measured and quantitatively compared with the available theoretical models. The scarcity of quantitative data is partially due to the strong three-dimensional character of bubble-induced streaming flows, which demands advanced velocimetry techniques. In this work, we present quantitative measurements of the flow generated by single and pairs of acoustically excited sessile microbubbles using a three-dimensional particle tracking technique. Using this novel experimental approach we are able to obtain the bubble's resonant oscillating frequency, study the boundaries of the linear oscillation regime, give predictions on the flow strength and the shear in the surrounding surface and study the flow and the stability of a two-bubble system. Our results show that velocimetry techniques are a suitable tool to make diagnostics on the dynamics of acoustically excited microbubbles

Nuclear WRAP53 promotes neuronal survival and functional recovery after stroke

Failure of neurons to efficiently repair DNA double-strand breaks (DSBs) contributes to cerebral damage after stroke. However, the molecular machinery that regulates DNA repair in this neurological disorder is unknown. Here, we found that DSBs in oxygen/glucose-deprived (OGD) neurons spatiotemporally correlated with the up-regulation of WRAP53 (WD40-encoding p53-antisense RNA), which translocated to the nucleus to activate the DSB repair response. Mechanistically, OGD triggered a burst in reactive oxygen species that induced both DSBs and translocation of WRAP53 to the nucleus to promote DNA repair, a pathway that was confirmed in an in vivo mouse model of stroke. Noticeably, nuclear translocation of WRAP53 occurred faster in OGD neurons expressing the Wrap53 human nonsynonymous single-nucleotide polymorphism (SNP) rs2287499 (c.202C>G). Patients carrying this SNP showed less infarct volume and better functional outcome after stroke. These results indicate that WRAP53 fosters DNA repair and neuronal survival to promote functional recovery after stroke

Bullet-induced synovitis as a cause of secondary osteoarthritis of the hip joint: A case report and review of literature

<p>Abstract</p> <p>Background</p> <p>With increasing prevalence of gunshot injuries we are seeing more patients with retained bullet fragments lodged in their bodies. Embedded lead bullets are usually considered inert after their kinetic energy has dissipated hence these are not removed routinely. However, exposure of any foreign body to synovial fluid may lead to rapid degradation and hence result in systemic absorption, causing local and systemic symptoms. We present the case of a thirty year old man who came to our out patient department with a history of progressive, severe hip pain ten years after a gun shot injury to his right hip.</p> <p>Conclusion</p> <p>The common belief that intraarticular bullets should not be removed has no benefit and may result in unwanted long term complications.</p

Testing matter effects in propagation of atmospheric and long-baseline neutrinos

We quantify our current knowledge of the size and flavor structure of the matter effects in the evolution of atmospheric and long-baseline neutrinos based solely on the analysis of the corresponding neutrino data. To this aim we generalize the matter potential of the Standard Model by rescaling its strength, rotating it away from the e-e sector, and rephasing it with respect to the vacuum term. This phenomenological parametrization can be easily translated in terms of non-standard neutrino interactions in matter. We show that in the most general case, the strength of the potential cannot be determined solely by atmospheric and long-baseline data. However its flavor composition is very much constrained and the present determination of the neutrino masses and mixing is robust under its presence. We also present an update of the constraints arising from this analysis in the particular case in which no potential is present in the e-mu and e-tau sectors. Finally we quantify to what degree in this scenario it is possible to alleviate the tension between the oscillation results for neutrinos and antineutrinos in the MINOS experiment and show the relevance of the high energy part of the spectrum measured at MINOS.Comment: PDFLaTeX file using JHEP3 class, 25 pages, 7 figures included. Accepted for publication in JHE

Laboratory evidence for proton energization by collisionless shock surfing

Charged particles can be accelerated to high energies by collisionless shock waves in astrophysical environments, such as supernova remnants. By interacting with the magnetized ambient medium, these shocks can transfer energy to particles. Despite increasing efforts in the characterization of these shocks from satellite measurements at Earth’s bow shock as well as powerful numerical simulations, the underlying acceleration mechanism or a combination thereof is still widely debated. Here we show that astrophysically relevant super-critical quasi-perpendicular magnetized collisionless shocks can be produced and characterized in the laboratory. We observe the characteristics of super-criticality in the shock profile as well as the energization of protons picked up from the ambient gas to hundreds of kiloelectronvolts. Kinetic simulations modelling the laboratory experiment identified shock surfing as the proton acceleration mechanism. Our observations not only provide direct evidence of early-stage ion energization by collisionless shocks but also highlight the role played by this particular mechanism in energizing ambient ions to feed further stages of acceleration. Furthermore, our results open the door to future laboratory experiments investigating the possible transition to other mechanisms, when increasing the magnetic field strength, or the effect that induced shock front ripples could have on acceleration processes

Automated Analysis of Cryptococcal Macrophage Parasitism Using GFP-Tagged Cryptococci

The human fungal pathogens Cryptococcus neoformans and C. gattii cause life-threatening infections of the central nervous system. One of the major characteristics of cryptococcal disease is the ability of the pathogen to parasitise upon phagocytic immune effector cells, a phenomenon that correlates strongly with virulence in rodent models of infection. Despite the importance of phagocyte/Cryptococcus interactions to disease progression, current methods for assaying virulence in the acrophage system are both time consuming and low throughput. Here, we introduce the first stable and fully characterised GFP–expressing derivatives of two widely used cryptococcal strains: C. neoformans serotype A type strain H99 and C. gattii serotype B type strain R265. Both strains show unaltered responses to environmental and host stress conditions and no deficiency in virulence in the macrophage model system. In addition, we report the development of a method to effectively and rapidly investigate macrophage parasitism by flow cytometry, a technique that preserves the accuracy of current approaches but offers a four-fold improvement in speed

Detailed characterization of a laboratory magnetized supercritical collisionless shock and of the associated proton energization

Collisionless shocks are ubiquitous in the Universe and are held responsible for the production of nonthermal particles and high-energy radiation. In the absence of particle collisions in the system, theory shows that the interaction of an expanding plasma with a pre-existing electromagnetic structure (as in our case) is able to induce energy dissipation and allow shock formation. Shock formation can alternatively take place when two plasmas interact, through microscopic instabilities inducing electromagnetic fields that are able in turn to mediate energy dissipation and shock formation. Using our platform in which we couple a rapidly expanding plasma induced by high-power lasers (JLF/Titan at LLNL and LULI2000) with high-strength magnetic fields, we have investigated the generation of a magnetized collisionless shock and the associated particle energization. We have characterized the shock as being collisionless and supercritical. We report here on measurements of the plasma density and temperature, the electromagnetic field structures, and the particle energization in the experiments, under various conditions of ambient plasma and magnetic field. We have also modeled the formation of the shocks using macroscopic hydrodynamic simulations and the associated particle acceleration using kinetic particle-in-cell simulations. As a companion paper to Yao et al. [Nat. Phys. 17, 1177-1182 (2021)], here we show additional results of the experiments and simulations, providing more information to allow their reproduction and to demonstrate the robustness of our interpretation of the proton energization mechanism as being shock surfing acceleration

Synthesis of some nucleosides derivatives from L- rhamnose with expected biological activity

Practical procedures for production of variously blocked compounds from L-rhamnose have been developed. These compounds are highly useful as indirect β-L-rhamnosyl donors. This approach represents a new method for the synthesis of aromatic nucleoside analogues and the synthesis of (3S, 4S, 5S, 6R) 3, 4, 5-triacetoxy-2-methyl-7,9-diaza-1-oxa-spiro [4,5]decane-10-one-8-thione (7)