Dry Matter Accumulation and Partitioning between Vegetative and Reproductive Organs in Alfalfa (\u3ci\u3eMedicago sativa\u3c/i\u3e L.)

This work investigated the partitioning of dry matter between vegetative and reproductive plant organs in alfalfa during the reproductive period under field conditions. Two French varieties (Europe and Magali) were studied. Both varieties showed similar growth pattern of the different plant organs in 1998 and 1999. The mean dry matter of vegetative organs (shoots and leaves) over the two years was higher in Europe (567g/m2) than Magali (470g/m2). No vegetative growth was observed during the reproductive period. The root organs (measured to a depth of 0.20 m) and the reproductive organs showed an increase in dry matter accumulation during the first 300 °Cd and 600 °Cd, respectively. It indicated that dry matter was preferentially partitioned to the reproductive organs during the first 600 °Cd. The root organs seem to be a competing sink during the early seed growth (200 °Cd to 300 °Cd). The dry matter partitioning was not affected by the year. Thus, when dry matter accumulation ceased only 30% in Europe and 27% in Magali of the aboveground dry weight was in the reproductive organs. The mean inflorescence weight reached its maximum after 450 °Cd from inflorescence flowering

Genetic diversity of HLA system in three populations from Sonora, Mexico: Ciudad Obregón, Hermosillo and rural Sonora

We studied HLA class I (HLA-A, -B) and class II (HLA-DRB1, -DQB1) alleles by PCR-SSP based typing in 439 Mexicans from the state of Sonora living in Ciudad Obregón (N = 143), Hermosillo (N = 99), and rural communities (N = 197) to obtain information regarding allelic and haplotypic frequencies. We find that the 13 most frequent haplotypes for the state of Sonora include nine Native American, three European and one Asian haplotypes. Admixture estimates revealed that the main genetic components in the state of Sonora are European (51.25 ± 2.90 by ML; 37.70 of European haplotypes) and Native American (43.35 ± 2.57 by ML; 39.64 of Native American haplotypes), while the African genetic component was less apparent (5.39 ± 2.54 by ML; 11.04 of African haplotypes)

Genetic diversity of HLA system in two populations from Sinaloa, Mexico: Culiacán and rural Sinaloa

We studied HLA class I (HLA-A, -B) and class II (HLA-DRB1, -DQB1) alleles by PCR-SSP based typing in 286 Mexicans from the state of Sinaloa living in Culiacán (N = 103) and rural communities (N = 183) to obtain information regarding allelic and haplotypic frequencies. We find that the most frequent haplotypes for the state of Sinaloa include ten Native American most probable ancestry and five European most probable ancestry haplotypes. The admixture estimates revealed that the main genetic components in the state of Sinaloa are European (62.39 ± 3.47) and Native American (37.61 ± 2.85), while the African genetic component was estimated as virtually absent (0.00 ± 1.86)

Genetic diversity of HLA system in four populations from Baja California, Mexico: Mexicali, La Paz, Tijuana and rural Baja California

We studied HLA class I (HLA-A, -B) and class II (HLA-DRB1, -DQB1) alleles by PCR-SSP based typing in 250 Mexicans from the states of Baja California Norte and Baja California Sur living in Mexicali (N = 100), La Paz (N = 75), Tijuana (N = 25) and rural communities (N = 50) to obtain information regarding allelic and haplotypic frequencies. The most frequent haplotypes for the Baja California region include nine Native American and five European haplotypes. Admixture estimates revealed that the main genetic components are European (50.45 ± 1.84 by ML; 42.03 of European haplotypes) and Native American (43.72 ± 2.36 by ML; 40.24 of Native American haplotypes), while the African genetic component was less apparent (5.83 ± 0.98 by ML; 9.36 of African haplotypes)

Comparison of morphological traits and molecular markers to analyse diversity and structure of alfalfa (Medicago sativa L.) cultivars

Diversity and structure of populations may differ substantially between morphological traits and molecular markers. Explanations of such discrepancies are crucial for further progress in breeding as well as for the maintenance of genetic resources. Our objective was to compare indices of among-cultivars differentiation for morphological traits (QST) and molecular markers (FST) in alfalfa (Medicago sativa), a legume forage species. Ten cultivars representing the Northern and Southern types commonly grown in Europe were investigated. For each cultivar, 40 genotypes were analysed with 16 SSR markers and four morphological traits measured in two locations and several cuts. QST values were in general high (0.02–0.39) compared to the differentiation observed with molecular markers (FST = 0.01), especially for growth habit, indicating that morphological traits were more efficient to structure the diversity than molecular markers. For morphological traits, a clear separation of Northern and Southern cultivars was observed, whereas for molecular markers, no clear structure was detected. Nevertheless, the grazing type cultivar Luzelle was separated from the rest of the cultivars for both morphological traits and molecular markers. Although pairwise differences between cultivars were significant for both morphological traits and molecular markers, the main part of the variation was found within cultivars. This large within-cultivar variation may be explained, besides the outcrossing reproductive mode and autotetraploid genetics, by the recent history of M. sativa domestication in Europe and the frequent seed exchanges. Selection for morphological traits (QST > FST) was achieved without modification of within-cultivar neutral diversity