Cala Ratjada

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Enhanced Labeling Techniques to Study the Cytoskeleton During Root Growth and Gravitropism

Gravity effects the growth and development of all living organisms. One of the most obvious manifestations of gravity's effects on biological systems lies in the ability of plants to direct their growth along a path that is dictated by the gravity vector (called gravitropism). When positioned horizontally, in florescence stems and hypocotyls in dicots, and pulvini in monocots, respond by bending upward whereas roots typically bend downward. Gravitropism allows plants to readjust their growth to maximize light absorption for photosynthesis and to more efficiently acquire water and nutrients form the soil. Despite its significance for plant survival, there are still major gaps in understanding the cellular and molecular processes by which plants respond to gravity. The major aim of this proposal was to develop improved fluorescence labeling techniques to aid in understanding how the cytoskeleton modulated plant responses to gravity

Assessment of an automated IoT-biofloc water quality management system in the Litopenaeus vannamei’s mortality and growth rate

Climate change greatly influenced aquaculture in the Philippines and the deteriorating water quality; in effect, fish production reduced drastically. Thus, fish farmers and aquaculture researchers have been searching for innovative technologies to address these issues. One technology is the biofloc systems. This technology uses a zero-water exchange and accumulates microorganisms that serves as a food source. In the biofloc, water quality management is highly recommended. This study aimed to assess the technical and operational effect on the shrimp growth and survival of the developed automated water quality management internet of things (IoT) system in a biofloc system. The hardware prototype comes with a mobile application which has features namely: account management, fish/shrimp profile, water quality management, auto feeding and manual controls. This study conducts experimental research which assessed the impact of an indoor pond biofloc with the developed prototype on the Litopenaeus vannamei’s growth and survival rates. Results were favorable to the biofloc system with the developed prototype, having a 10% higher survival rate and 3.2% higher growth rate, compared to the traditional recirculating aquaculture systems. Adapting the biofloc and the IoT prototype, a fish farmer may earn P78,300 pesos for each white leg shrimp (Litopenaeus vannamei) harvesting period

A LIGHTWEIGHT MULTI-PERSON POSE ESTIMATION SCHEME BASED ON JETSON NANO

As the basic technology of human action recognition, pose estimation is attracting more and more researchers' attention, while edge application scenarios pose a higher challenge. This paper proposes a lightweight multi-person pose estimation scheme to meet the needs of real-time human action recognition on the edge end. This scheme uses AlphaPose to extract human skeleton nodes, and adds ResNet and Dense Upsampling Revolution to improve its accuracy. Meanwhile, we use YOLO to enhance AlphaPose’s support for multi-person pose estimation, and optimize the proposed model with TensorRT. In addition, this paper sets Jetson Nano as the Edge AI deployment device of the proposed model and successfully realizes the model migration to the edge end. The experimental results show that the speed of the optimized object detection model can reach 20 FPS, and the optimized multi-person pose estimation model can reach 10 FPS. With the image resolution of 320×240, the model’s accuracy is 73.2%, which can meet the real-time requirements. In short, our scheme can provide a basis for lightweight multi-person action recognition scheme on the edge end

Momentos de refundación

Sombragris; Blancaflor (2002). Momentos de refundación. La madriguera. (50):71-72. http://hdl.handle.net/10251/42091.Importación Masiva71725

Borrarse, borrarse

Blancaflor; Sombragris (2001). Borrarse, borrarse. La madriguera. (38):57-58. http://hdl.handle.net/10251/41964.Importación Masiva57583

Divergence and Redundancy in CSLD2 and CSLD3 Function During Arabidopsis Thaliana Root Hair and Female Gametophyte Development

The Arabidopsis cellulose synthase-like D (CSLD) 2 and 3 genes are known to function in root hair development. Here, we show that these genes also play a role in female gametophyte development because csld2 csld3 double mutants were observed to have low seed set that could be traced to defects in female transmission efficiency. Cell biological studies of csld2 csld3 ovules showed synergid cell degeneration during megagametogenesis and reduced pollen tube penetration during fertilization. Although CSLD2 and CSLD3 function redundantly in female gametophyte development, detailed analyses of root hair phenotypes of progeny from genetic crosses between csld2 and csld3, suggest that CSLD3 might play a more prominent role than CSLD2 in root hair development. Phylogenetic and gene duplication studies of CSLD2 and CSLD3 homologs in Arabidopsis lyrata, Populus, Medicago, maize, and Physcomitrella were further performed to investigate the course of evolution for these genes. Our analyses indicate that the ancestor of land plants possibly contained two copies of CSLD genes, one of which developed into the CSLD5 lineage in flowering plants, and the other formed the CSLD1/2/3/4 clade. In addition, CSLD2 and CSLD3 likely originated from a recent genome-wide duplication event explaining their redundancy. Moreover, sliding-window dN/dS analysis showed that most of the coding regions of CSLD2 and CSLD3 have been under strong purifying selection pressure. However, the region that encodes the N-terminus of CSLD3 has been under relatively relaxed selection pressure as indicated by its high dN/dS value, suggesting that CSLD3 might have gained additional functions through more frequent non-synonymous sequence changes at the N-terminus, which could partly explain the more prominent role of CSLD3 during root hair development compared to CSLD2

Necesitamos que la política se configure de otra forma (a propósito de los asesinos están entre nosotros, de Juan Miguel Company)

Blancaflor; Sombragris (2001). Necesitamos que la política se configure de otra forma (a propósito de los asesinos están entre nosotros, de Juan Miguel Company). La madriguera. (42):54-55. http://hdl.handle.net/10251/42001.Importación Masiva54554

Algunas reflexiones sobre las nuevas representaciones de la alteridad. A proósito de Kasbah, de Mariano Barroso

Sombragris; Blancaflor (2000). Algunas reflexiones sobre las nuevas representaciones de la alteridad. A proósito de Kasbah, de Mariano Barroso. La madriguera. (33):85-88. http://hdl.handle.net/10251/41914.Importación Masiva85883

Auxin, actin and growth of the Arabidopsis thaliana primary root

Summary To understand how auxin regulates root growth, we quantified cell division and elemental elongation, and examined actin organization in the primary root of Arabidopsis thaliana. In treatments for 48 h that inhibited root elongation rate by 50%, we find that auxins and auxin-transport inhibitors can be divided into two classes based on their effects on cell division, elongation and actin organization. Indole acetic acid (IAA), 1-naphthalene acetic acid (NAA) and tri-iodobenzoic acid (TIBA) inhibit root growth primarily through reducing the length of the growth zone rather than the maximal rate of elemental elongation and they do not reduce cell production rate. These three compounds have little effect on the extent of filamentous actin, as imaged in living cells or by chemical fixation and immuno-cytochemistry, but tend to increase actin bundling. In contrast, 2,4-dichlorophenoxy-acetic acid (2,4-D) and naphthylphthalamic acid (NPA) inhibit root growth primarily by reducing cell production rate. These compounds remove actin and slow down cytoplasmic streaming, but do not lead to mislocalization of the auxin-efflux proteins, PIN1 or PIN2. The effects of 2,4-D and NPA were mimicked by the actin inhibitor, latrunculin B. The effects of these compounds on actin were also elicited by a 2 h treatment at higher concentration but were not seen in two mutants, eir1-1 and aux1-7, with deficient auxin transport. Our results show that IAA regulates the size of the root elongation zone whereas 2,4-D affects cell production and actin-dependent processes; and, further, that elemental elongation and localization of PINs are appreciably independent of actin