I am tired of having to prove that my husband was dead. Dealing with practical matters in bereavement and the impact on the bereaved

This paper reports on experiences of dealing with practical matters after death. Semi structured interviews with bereaved individuals were thematically analyzed. Within the theme of coping, dealing with practical matters was a significant stressor and was found to be extremely challenging, time consuming, and to negatively impact on mental and emotional well-being. This study adds new insights on the challenges experienced by the bereaved when attending to practical matters and may help to inform the design of bereavement support, inform standard operating procedures of businesses, and government bereavement leave legislation

Design and development of an experimental setup of electrically powered spinning rotor blades in icing wind tunnel and preliminary testing with surface coatings as hybrid protection solution

In order to study ice protection systems for rotating blades, a new experimental setup has been developed at the Anti-Icing Materials International Laboratory (AMIL). This system consists of two small-scale rotating blades in a refrigerated icing wind tunnel where atmospheric icing can be simulated. Power is brought to the blades through a slip ring, through which the signals of the different sensors that are installed on the blades also pass. As demonstrated by the literature review, this new setup will address the need of small-scale wind tunnel testing on electrically powered rotating blades. To test the newly designed apparatus, preliminary experimentation is done on a hybrid ice protection system. Electrothermal protection is combined with different surface coatings to measure the impact of those coatings on the power consumption of the system. In anti-icing mode, the coatings tested did not reduce the power consumption on the system required to prevent ice from accumulating on the leading edge. The coatings however, due to their hydrophobic/superhydrophobic nature, reduced the power required to prevent runback ice accumulation when the leading edge was protected. One of the coatings did not allow any runback accumulation, limiting the power to protect the whole blades to the power required to protect solely the leading edge, resulting in a potential 40% power reduction for the power consumption of the system. In de-icing mode, the results with all the substrates tested showed similar power to achieve ice shedding from the blade. Since the coatings tested have a low icephobicity, it would be interesting to perform additional testing with icephobic coatings. Also, a small unheated zone at the root of the blade prevented complete ice shedding from the blade. A small part of the ice layer was left on the blade after testing, meaning that a cohesive break had to occur within the ice layer, and therefore impacting the results. Improvements to the setup will be done to remedy the situation. Those preliminary testing performed with the newly developed test setup have demonstrated the potential of this new device which will now allow, among other things, to measure heat transfer, force magnitudes, ice nucleation, and thermal equilibrium during ice accretion, with different innovative thermal protection systems (conductive coating, carbon nanotubes, impulse, etc.) as well as mechanical systems. The next step, following the improvements, is to measure forced convection on a thermal ice protection system with and without precipitation and to test mechanical ice protection systems

An experimental investigation of the convective heat transfer on a small helicopter rotor with anti-icing and de-icing test setups

Successful icing/de-icing simulations for rotorcraft require a good prediction of the convective heat transfer on the blade’s surface. Rotorcraft icing is an unwanted phenomenon that is known to cause flight cancelations, loss of rotor performance and severe vibrations that may have disastrous and deadly consequences. Following a series of experiments carried out at the Anti-icing Materials International Laboratory (AMIL), this paper provides heat transfer measurements on heated rotor blades, under both the anti-icing and de-icing modes in terms of the Nusselt Number (Nu). The objective is to develop correlations for the Nu in the presence of (1) an ice layer on the blades (NuIce) and (2) liquid water content (LWC) in the freestream with no ice (NuWet). For the sake of comparison, the NuWet and the NuIce are compared to heat transfer values in dry runs (NuDry). Measurements are reported on the nose of the blade-leading edge, for three rotor speeds (Ω) = 500, 900 and 1000 RPM; a pitch angle (θ) = 6°; and three different radial positions (r/R), r/R = 0.6, 0.75 and 0.95. The de-icing tests are performed twice, once for a glaze ice accretion and another time for rime ice. Results indicate that the NuDry and the NuWet directly increased with V∝, r/R or Ω, mainly due to an increase in the Reynolds number (Re). Measurements indicate that the NuWet to NuDry ratio was always larger than 1 as a direct result of the water spray addition. NuIce behavior was different and was largely affected by the ice thickness (tice) on the blade. However, the ice acted as insulation on the blade surface and the NuIce to NuDry ratio was always less than 1, thus minimizing the effect of convection. Four correlations are then proposed for the NuDry, the NuWet and the NuIce, with an average error between 3.61% and 12.41%. The NuDry correlation satisfies what is expected from heat transfer near the leading edge of an airfoil, where the NuDry correlates well with Re0.52

A Screen of FDA-Approved Drugs Identifies Inhibitors of Protein Tyrosine Phosphatase 4A3 (PTP4A3 or PRL-3)

Protein tyrosine phosphatase 4A3 (PTP4A3 or PRL-3) is highly expressed in a variety of cancers, where it promotes tumor cell migration and metastasis leading to poor prognosis. Despite its clinical significance, small molecule inhibitors of PRL-3 are lacking. Here, we screened 1443 FDA-approved drugs for their ability to inhibit the activity of the PRL phosphatase family. We identified five specific inhibitors for PRL-3 as well as one selective inhibitor of PRL-2. Additionally, we found nine drugs that broadly and significantly suppressed PRL activity. Two of these broad-spectrum PRL inhibitors, Salirasib and Candesartan, blocked PRL-3-induced migration in human embryonic kidney cells with no impact on cell viability. Both drugs prevented migration of human colorectal cancer cells in a PRL-3 dependent manner and were selective towards PRLs over other phosphatases. In silico modeling revealed that Salirasib binds a putative allosteric site near the WPD loop of PRL-3, while Candesartan binds a potentially novel targetable site adjacent to the CX5R motif. Inhibitor binding at either of these sites is predicted to trap PRL-3 in a closed conformation, preventing substrate binding and inhibiting function

Challenges in linking administrative data for monitoring bloodstream infection in neonatal units in England and Wales

Introduction Monitoring risk-adjusted trends of neonatal bloodstream infection (BSI) is vital and linkage of neonatal electronic health records to national infection surveillance enables this. We demonstrate why changes in data quality and collection methods over time must be accounted for to minimise spurious findings. Objectives and Approach First, we determined the effect of a system change in 2014 (changed from only clinically relevant BSI to automated reporting of all BSI), by investigating changes in number of all BSI and BSI excluding the contaminants coagulase-negative staphylococci for infants aged <1 year reported to infection surveillance, using interrupted-time-series Poisson regression. Second, we evaluated the impact of changes in identifier completeness over time in each database, and determined variation in infection rates according to linkage method (deterministic linkage on NHS number or probabilistic linkage). Third, we will use multiple imputation when link status cannot be determined due to missing identifiers. Results The number of BSI reported to infection surveillance system following the change in data collection increased by 34% (incidence rate ratio (IRR) of 1.34, 95% confidence interval 1.28-1.40) for all BSI compared to 19% (IRR 1.19, 1.12-1.27) excluding coagulase-negative staphylococci. Completeness of NHS number in infection surveillance increased from 69% (3,296/4,792) in 2010 to 92% (3,037/3,307) in 2017. We linked 12,003 neonatal admissions to 15,571 BSI episodes (2% of 497,936 admissions and 41% of 37,660 BSI). The proportion of links that were deterministic changed from 83% (1,089/1,307) in 2010 to 96% (968/1,008) in 2017. There were 12,094 BSI for which the link status could not be determined due to missing identifiers; multiple imputation will be used to determine if any are links. Conclusion/Implications Spurious trends in infection incidence can arise from changes in data collection and quality, impacting the quality of linkage to clinical data. Data quality and system changes must be explored in each source dataset before analysis. Probabilistic linkage and imputation of missing data minimises spurious findings due to data quality

Challenges in linking administrative data for monitoring bloodstream infection in neonatal units in England and Wales

Monitoring risk-adjusted trends of neonatal bloodstream infection is vital and linkage of neonatal electronic health records to national infection surveillance enables this. We demonstrate why changes in data quality over time must be accounted for to minimise spurious findings. First, we evaluated the impact of changes in identifier completeness over time in each database, and determined variation in infection rates according to linkage method (deterministic linkage on NHS number or probabilistic linkage). Second, we will use multiple imputation when link status cannot be determined due to missing identifiers. Completeness of NHS number in infection surveillance increased from 69% (3,296/4,792) in 2010 to 92% (3,037/3,307) in 2017. We linked 12,003 neonatal admissions to 15,571 infection episodes (2% of 497,936 admissions and 41% of 37,660 infections). The proportion of links that were deterministic changed from 83% (1,089/1,307) in 2010 to 96% (968/1,008) in 2017. Link status could not be determined for 12,094 infections due to missing identifiers; multiple imputation will be used to determine if any are links. Spurious infection incidence rates can arise from changes in data quality, impacting the quality of linkage to clinical data. Linkage and imputation of missing data minimises spurious findings due to data quality

Reactivity of vanadium oxytrichloride with [beta]-diketones and diesters as precursors for vanadium nitride and carbide

Vanadium(V) oxytrichloride was reacted with 2,4-pentanedione, diethyl malonate, and diethyl succinate under inert conditions, forming compounds: dichloro(oxo)(2,4-pentanedione) vanadium(V) [1], dichloro(oxo)(diethyl malonate) vanadium(IV) [2] and dichloro(oxo)(diethyl succinate) vanadium(IV) [3]. Compounds 1–3 are coordinated to the vanadium centre through the two carbonyl oxygen atoms of the bidentate ligand. It was determined by X-ray crystallography that the structures of the resulting complexes were significantly different, resulting in a monomeric complex (1), a tetrameric ring (2) and a 1D coordination polymer (3). Following the synthesis and isolation of 1–3, they were tested as precursors for vanadium nitride and vanadium carbide by annealing under nitrogen and argon respectively at 1200 °C for 24 h. The resulting materials were characterised by: XRD, EDS, XPS and TEM

Expression of Protease-Activated Receptor 1 and 2 and Anti-Tubulogenic Activity of Protease-Activated Receptor 1 in Human Endothelial Colony-Forming Cells

Endothelial colony-forming cells (ECFCs) are obtained from the culture of human peripheral blood mononuclear cell (hPBMNC) fractions and are characterised by high proliferative and pro-vasculogenic potential, which makes them of great interest for cell therapy. Here, we describe the detection of protease-activated receptor (PAR) 1 and 2 amongst the surface proteins expressed in ECFCs. Both receptors are functionally coupled to extracellular signal-regulated kinase (ERK) 1 and 2, which become activated and phosphorylated in response to selective PAR1- or PAR2-activating peptides. Specific stimulation of PAR1, but not PAR2, significantly inhibits capillary-like tube formation by ECFCs in vitro, suggesting that tubulogenesis is negatively regulated by proteases able to stimulate PAR1 (e.g. thrombin). The activation of ERKs is not involved in the regulation of tubulogenesis in vitro, as suggested by use of the MEK inhibitor PD98059 and by the fact that PAR2 stimulation activates ERKs without affecting capillary tube formation. Both qPCR and immunoblotting showed a significant downregulation of vascular endothelial growth factor 2 (VEGFR2) in response to PAR1 stimulation. Moreover, the addition of VEGF (50–100 ng/ml) but not basic Fibroblast Growth Factor (FGF) (25–100 ng/ml) rescued tube formation by ECFCs treated with PAR1-activating peptide. Therefore, we propose that reduction of VEGF responsiveness resulting from down-regulation of VEGFR2 is underlying the anti-tubulogenic effect of PAR1 activation. Although the role of PAR2 remains elusive, this study sheds new light on the regulation of the vasculogenic activity of ECFCs and suggests a potential link between adult vasculogenesis and the coagulation cascade

Analysing NSW state policy for child obesity prevention: strategic policy versus practical action

There is increasing worldwide recognition of the need for government policies to address the recent increases in the incidence and prevalence of childhood obesity. The complexity and inter-relatedness of the determinants of obesity pose a genuine policy challenge, both scientifically and politically. This study examines the characteristics of one of the early policy responses, the NSW Government\u27s Prevention of Obesity in Children and Young People: NSW Government Action Plan 2003-2007 (GAP), as a case study, assessing it in terms of its content and capacity for implementation. This policy was designed as an initial set of practical actions spanning five government sectors. Most of the policy actions fitted with existing implementation systems within NSW government, and reflected an incremental approach to policy formulation and implementation. As a case study, the NSW Government Action Plan illustrates that childhood obesity policy development and implementation are at an early stage. This policy, while limited, may have built sufficient commitment and support to create momentum for more strategic policy in the future. A more sophisticated, comprehensive and strategic policy which can also be widely implemented and evaluated should now be built on this base

Cohesin SMC1β protects telomeres in meiocytes

Telomeres fail to attach to the nuclear envelope and lose structural integrity in cells lacking SMC1β