Selection for resistance against root pathogens in a pea composite cross

The possibility of improving resistance in pea against the root pathogen Aphanomyces euteiches using composite cross as a breeding and selection method was examined. In order to maintain acceptable agricultural features and high yield 6 out of the 8 parental varieties in the present composite-cross were commercially grown varieties. Populations of the composite cross were grown up to five generations with selection pressure in soil heavily infested with pea root pathogens or without selection pressure on soil free of pea root pathogens. Yield of populations of the F9 and F10 generations of the composite cross grown with selection pressure was on average 35% higher than that of the population obtained without selection pressure as well as the average yield of the 8 parentals of the composite cross, which were of similar magnitude. In healthy soil the yield was overall higher than in the pathogen-infested soil, but yield did not differ between the populations from the composite cross with and without selection pressure, which were also similar to the average yield of the 8 different parentals. Recombinant inbred lines (RILs) randomly selected from the F10 population with selection pressure developed 23% less root rot than the corresponding F10 population without selection pressure, when grown in field soil heavily infested with pea root pathogens. Surprisingly, greenhouse pot experiments with pure cultures of the pea root pathogen A. euteiches resulted in higher root disease, in RILs from populations with selection pressure than from corresponding RILs without selection pressure. Problems related to greenhouse screening for resistance is discussed as well as the possibilities of using composite cross as a method to improve resistance against root diseases in grain legumes

Using equilibrium frequencies in models of sequence evolution

BACKGROUND: The f factor is a new parameter for accommodating the influence of both the starting and ending states in the rate matrices of "generalized weighted frequencies" (+gwF) models for sequence evolution. In this study, we derive an expected value for f, starting from a nearly neutral model of weak selection, and then assess the biological interpretation of this factor with evolutionary simulations. RESULTS: An expected value of f = 0.5 (i.e., equal dependency on the starting and ending states) is derived for sequences that are evolving under the nearly neutral model of this study. However, this expectation is sensitive to violations of its underlying assumptions as illustrated with the evolutionary simulations. CONCLUSION: This study illustrates how selection, drift, and mutation at the population level can be linked to the rate matrices of models for sequence evolution to derive an expected value of f. However, as f is affected by a number of factors that limit its biological interpretation, this factor should normally be estimated as a free parameter rather than fixed a priori in a +gwF analysis

Specificity of soil-borne pathogens on grain legumes

Specificity of soil-borne legume pathogens on pea, lupin and faba bean is currently investigated in fields where grain legumes are intensively cultivated. The study has so far lead to the following conclusions -Legume host-pathogen interactions demonstrate specificity of pathogen populations particularly in pea and lupin. -A. euteiches rot root was specified to pea in Denmark as root rot symptoms and oospores of the pathogen never were observed in roots of faba bean and lupin -F. oxysporum followed by F. solani were most frequently isolated from plant roots in plots highly infested by lupin pathogens -F. avenaceum was most frequently isolated from plant roots in plots highly infested by pea pathogens -Pathogenicity tests showed F. solani followed by F. avenaceum to be the most pathogenic Fusarium species on pea while F. avenaceum was the most destructive pathogen on faba bean. In contrast F. avenaceum was non-pathogenic on lupin

Parat til uddannelse?

Ungdomsuddannelse til alle har været på den politiske agenda i mange år, og der er enighed om, at der skal satses aktivt på at få flere gennem uddannelsessystemet, hvis vi i Danmark skal være rustet til at møde globaliseringens muligheder og udfordringer. Men frafaldet er stort. Hver femte unge gennemfører ikke en ungdomsuddannelse, og der er grund til at se på, hvordan vi kan hjælpe flere unge med at blive ”parate til uddannelse”. Denne artikel præsenter nogle af de vigtigste udfordringer for de unge frafaldstruede og anbefaler en bedre afklaring af deres uddannelsesvalg

Measuring perceived air quality and intensity by means of sensor systems:The European project SysPAQ

International audienceAt present, indoor air quality is assessed exclusively by human panels. As this method is time consuming and cost intensive, little attention is paid to indoor air quality in the planning and operation of buildings. In recent years multi-gas sensor systems have been developed in order to mimic the human sense of smell. These systems comprise an array of gas sensors, with sensors of different sensitivity and selectivity, and a data processing unit. Up to now the sensors are not sensitive enough to mimic the perception of a human being. To further the development of these systems, the European research project SysPAQ (Innovative Sensor System for Measuring Perceived Air Quality and Brand Specific Odours) was started in September 2006. It will end in September 2009

Archives of human-dog relationships: Genetic and stable isotope analysis of Arctic fur clothing

Among Indigenous populations of the Circumpolar North, domestic dogs (Canis familiaris) were social actors aiding in traction and subsistence activities. Less commonly, dogs fulfilled a fur-bearing role in both the North American and Siberian Arctic. Examples of garments featuring dog skins were collected during the 19th-20th centuries and are now curated by the National Museum of Denmark. We sequenced the mitochondrial genomes of macroscopically identified dog skin garments. We conducted stable carbon and nitrogen isotope ratio analysis of the dog furs and of fur samples from contemporaneous pelts of Arctic (C. lupus arctos) and grey (C. lupus) wolves. Despite the presence of biocides used to protect the fur clothing during storage, we extracted well-preserved DNA using a minimally-invasive sampling protocol. Unexpectedly, the mtDNA genomes of one-third of the samples were consistent with wild taxa, rather than domestic dogs. The strong marine component in the diets of North American dogs distinguished them from Greenland and Canadian wolves, but Siberian dogs consumed diets that were isotopically similar to wild species. We found that dog provisioning practices were variable across the Arctic, but in all cases, involved considerable human labor

The Western Denmark Cardiac Computed Tomography Registry:a review and validation study

BACKGROUND: As a subregistry to the Western Denmark Heart Registry (WDHR), the Western Denmark Cardiac Computed Tomography Registry (WDHR-CCTR) is a clinical database established in 2008 to monitor and improve the quality of cardiac computed tomography (CT) in Western Denmark. OBJECTIVE: We examined the content, data quality, and research potential of the WDHR-CCTR. METHODS: We retrieved 2008–2012 data to examine the 1) content; 2) completeness of procedure registration using the Danish National Patient Registry as reference; 3) completeness of variable registration comparing observed vs expected numbers; and 4) positive predictive values as well as negative predictive values of 19 main patient and procedure variables. RESULTS: By December 31, 2012, almost 22,000 cardiac CTs with up to 40 variables for each procedure have been registered. Of these, 87% were coronary CT angiography performed in patients with symptoms indicative of coronary artery disease. Compared with the Danish National Patient Registry, the overall procedure completeness was 72%. However, an additional medical record review of 282 patients registered in the Danish National Patient Registry, but not in the WDHR-CCTR, showed that coronary CT angiographies accounted for only 23% of all nonregistered cardiac CTs, indicating >90% completeness of coronary CT angiographies in the WDHR-CCTR. The completeness of individual variables varied substantially (range: 0%–100%), but was >85% for more than 70% of all variables. Using medical record review of 250 randomly selected patients as reference standard, the positive predictive value for the 19 variables ranged from 89% to 100% (overall 97%), whereas the negative predictive value ranged from 97% to 100% (overall 99%). Stratification by center status showed consistently high positive and negative predictive values for both university (96%/99%) and nonuniversity centers (97%/99%). CONCLUSION: WDHR-CCTR provides ongoing prospective registration of all cardiac CTs performed in Western Denmark since 2008. Overall, the registry data have a high degree of completeness and validity, making it a valuable tool for clinical epidemiological research