Estimation de l'équivalent en eau du couvert nival au moyen d'images radar satellitaires

L'objectif de cette étude est de vérifier le potentiel des images radar à synthèse d'ouverture (RSO) pour estimer l'équivalent en eau du couvert nival sur le bassin de la rivière La Grande (Baie de James, Québec). Il s'agit d'un milieu dominé par une forêt ouverte d'épinettes noires, des brûlis et des tourbières. Cette information intéresse grandement Hydro-Québec qui gère plusieurs installations hydro-électriques dans cette région subarctique. Durant deux ans, six campagnes de terrain ont été réalisées sur le bassin de la rivière La Grande et une dizaine d'images RSO du satellite européen ERS-1 ont été acquises, étalonnées et géoréférencées, afin de déterminer la relation entre les coefficients de rétrodiffusion des images radar (hiver et automne) et la résistance thermique du couvert nival. Cette relation constitue la première partie d'un algorithme d'estimation de l'équivalent en eau. Elle utilise plus spécifiquement le rapport de rétrodiffusion, qui est la différence entre une image avec neige et une image sans neige. La deuxième partie de cette algorithme déduit l'équivalent en eau du couvert de neige à partir de sa résistance thermique et de sa densité, en se basant sur la relation physique établie par les mesures de terrain. L'équivalent en eau du couvert nival a donc été estimé pour quatre images de février et mars 1994 et 1995. L'erreur moyenne sur l'estimation de l'équivalent en eau de la neige au sol est de 2% à 3% (-5 à 7mm) sur l'ensemble des sites d'échantillonnage avec un écart-type de 14 à 19% (-35 à 45mm). Ces résultats ont encouragé Hydro-Québec à poursuivre la recherche avec les données du satellite canadien RADARSAT (opérationnel depuis le 1er avril 1996) et à développer un prototype pour la cartographie de l'équivalent en eau du couvert nival à partir d'images radar.The goal of this study was to evaluate the potential of synthetic aperture radar (SAR) images for estimating the snow water equivalent (SWE) on the La Grande river watershed (James Bay area, Québec). This information is of major interest for Hydro-Québec, which exploits many hydroelectric complexes throughout this subarctic region. The La Grande watershed is composed of moderately dense to opened black spruce forests, opened areas, burned areas and peat bogs. Over two years (1994-1995), six field campaigns were carried out on a study site located between the LG4 and Laforge1 reservoir, in the center of the La Grande river watershed. The field measurements were of two types: 20 snow lines (depth, snow water equivalent (SWE), density) and 8 snow profiles (depth, density, grain size, temperature, dielectric constant). With these data, the thermal resistance of the snowpack was calculated for every test-site, using the depth, density and thermal conductivity of each layer. Concurrently, more than 10 SAR images (European Satellite ERS-1) of the study site were acquired, calibrated and georeferenced. The backscattering coefficients of all winter images were extracted. Using a reference image (snow-free), backscattering ratios were calculated. They are the difference between a winter image and a snow-free image. This process is used to reduce the impact of vegetation and topography. Then, the relationship between the backscattering ratios and the snowpack thermal resistance of february and march 1994 are established, as the first part of an algorithm developed to estimate the snow water equivalent. The second part of the algorithm infers the snowpack water equivalent from its thermal resistance and density, based on the physical relationship established with field data. This approach is based on studies conducted by INRS-Eau in a southern Quebec agricultural area (BERNIER and FORTIN (1998)). The hypothesis are based on the following: - The snowpack characteristics influence the underlying soil temperature;- The dielectric constant of the soil varies with the soil temperature under 0°C;- The radar signal is influenced by the soil dielectric constant;- Thus, the snowpack characteristics (thermal resistance) influence the radar signal. However, due to variations of soil humidity on the date of the reference image (september 1994), two slightly different relationships were obtained. One for open areas and open forests and one for burned areas and peat bogs. This shows the importance of using a good reference image, with homogeneous soil conditions. It could be better to obtain an image later in the fall, when the soil is frozen. The relationships established here are preliminary, as they use a small dataset. It is estimated that a better regression should be obtained with the acquisition of more images and with a greater range of snow characteristics. However, the algorithm is applied to test the applicability of the method. First, the algorithm was applied on the test-sites, using the images from February and March of 1994 and 1995. The mean error on the estimation of the snow water equivalent is 2% to 3% ( 5 to 7mm), with a deviation of 14% to 19% ( 35 to 45mm). The results are comparable for both years, even if the algorithm is based on 1994 data only. Secondly, the algorithm is applied on the whole images. A classification of a Landsat-TM image is used to identify the land cover of every pixel, which determines the regression and the snow density to be used in the algorithm. Four maps of the SWE are produced and resampled to a resolution of 500m. These are compared with the field measurements from the four nearest Hydro-Quebec snow survey sites. The SWE measured by Hydro-Quebec are all within the most dominant SWE class of each map. Further validation of the results will be possible when the algorithm can be applied on a sub-watershed, which is the actual scale used by Hydro-Quebec. However, the results of this study were sufficiently promising to Hydro-Quebec to support a follow up research with data from the canadian satellite RADARSAT (operational since april 1996). Meanwhile, to improve the algorithm, it is important to obtain a good and homogeneous reference image, to better assess the impact of the land cover and to acquire a dataset with a greater range of snow characteristics

Stroke, thromboembolism and bleeding in patients with atrial fibrillation according to the EHRA valvular heart disease classification

International audienc

Ionic and osmotic relations in quinoa (Chenopodium quinoa Willd.) plants grown at various salinity levels

Ionic and osmotic relations in quinoa (Chenopodium quinoa Willd.) were studied by exposing plants to six salinity levels (0–500 mM NaCl range) for 70 d. Salt stress was administered either by pre-mixing of the calculated amount of NaCl with the potting mix before seeds were planted or by the gradual increase of NaCl levels in the irrigation water. For both methods, the optimal plant growth and biomass was achieved between 100 mM and 200 mM NaCl, suggesting that quinoa possess a very efficient system to adjust osmotically for abrupt increases in NaCl stress. Up to 95% of osmotic adjustment in old leaves and between 80% and 85% of osmotic adjustment in young leaves was achieved by means of accumulation of inorganic ions (Na+, K+, and Cl–) at these NaCl levels, whilst the contribution of organic osmolytes was very limited. Consistently higher K+ and lower Na+ levels were found in young, as compared with old leaves, for all salinity treatments. The shoot sap K+ progressively increased with increased salinity in old leaves; this is interpreted as evidence for the important role of free K+ in leaf osmotic adjustment under saline conditions. A 5-fold increase in salinity level (from 100 mM to 500 mM) resulted in only a 50% increase in the sap Na+ content, suggesting either a very strict control of xylem Na+ loading or an efficient Na+ removal from leaves. A very strong correlation between NaCl-induced K+ and H+ fluxes was observed in quinoa root, suggesting that a rapid NaCl-induced activation of H+-ATPase is needed to restore otherwise depolarized membrane potential and prevent further K+ leak from the cytosol. Taken together, this work emphasizes the role of inorganic ions for osmotic adjustment in halophytes and calls for more in-depth studies of the mechanisms of vacuolar Na+ sequestration, control of Na+ and K+ xylem loading, and their transport to the shoot

Exogenous WNT5A and WNT11 proteins rescue CITED2 dysfunction in mouse embryonic stem cells and zebrafish morphants

Mutations and inadequate methylation profiles of CITED2 are associated with human congenital heart disease (CHD). In mouse, Cited2 is necessary for embryogenesis, particularly for heart development, and its depletion in embryonic stem cells (ESC) impairs cardiac differentiation. We have now determined that Cited2 depletion in ESC affects the expression of transcription factors and cardiopoietic genes involved in early mesoderm and cardiac specification. Interestingly, the supplementation of the secretome prepared from ESC overexpressing CITED2, during the onset of differentiation, rescued the cardiogenic defects of Cited2-depleted ESC. In addition, we demonstrate that the proteins WNT5A and WNT11 held the potential for rescue. We also validated the zebrafish as a model to investigate cited2 function during development. Indeed, the microinjection of morpholinos targeting cited2 transcripts caused developmental defects recapitulating those of mice knockout models, including the increased propensity for cardiac defects and severe death rate. Importantly, the co-injection of anti-cited2 morpholinos with either CITED2 or WNT5A and WNT11 recombinant proteins corrected the developmental defects of Cited2-morphants. This study argues that defects caused by the dysfunction of Cited2 at early stages of development, including heart anomalies, may be remediable by supplementation of exogenous molecules, offering the opportunity to develop novel therapeutic strategies aiming to prevent CHD.Agência financiadora: Fundação para a Ciência e a Tecnologia (FCT) Comissão de Coordenação e Desenvolvimento Regional do Algarve (CCDR Algarve) ALG-01-0145-FEDER-28044; DFG 568/17-2 Algarve Biomedical Center (ABC) Municipio de Louléinfo:eu-repo/semantics/publishedVersio

WNT signalling in prostate cancer

Genome sequencing and gene expression analyses of prostate tumours have highlighted the potential importance of genetic and epigenetic changes observed in WNT signalling pathway components in prostate tumours-particularly in the development of castration-resistant prostate cancer. WNT signalling is also important in the prostate tumour microenvironment, in which WNT proteins secreted by the tumour stroma promote resistance to therapy, and in prostate cancer stem or progenitor cells, in which WNT-β-catenin signals promote self-renewal or expansion. Preclinical studies have demonstrated the potential of inhibitors that target WNT receptor complexes at the cell membrane or that block the interaction of β-catenin with lymphoid enhancer-binding factor 1 and the androgen receptor, in preventing prostate cancer progression. Some WNT signalling inhibitors are in phase I trials, but they have yet to be tested in patients with prostate cancer

Short and long term retention in antiretroviral care in health facilities in rural Malawi and Zimbabwe.

Despite the successful scale-up of ART services over the past years, long term retention in ART care remains a major challenge, especially in high HIV prevalence and resource-limited settings. This study analysed the short (<12 months) and long (>12 months) term retention on ART in two ART programmes in Malawi (Thyolo district) and Zimbabwe (Buhera district)

WNT signaling regulates self-renewal and differentiation of prostate cancer cells with stem cell characteristics

Prostate cancer cells with stem cell characteristics were identified in human prostate cancer cell lines by their ability to form from single cells self-renewing prostaspheres in non-adherent cultures. Prostaspheres exhibited heterogeneous expression of proliferation, differentiation and stem cell-associated makers CD44, ABCG2 and CD133. Treatment with WNT inhibitors reduced both prostasphere size and self-renewal. In contrast, addition of Wnt3a caused increased prostasphere size and self-renewal, which was associated with a significant increase in nuclear Β-catenin, keratin 18, CD133 and CD44 expression. As a high proportion of LNCaP and C4-2B cancer cells express androgen receptor we determined the effect of the androgen receptor antagonist bicalutamide. Androgen receptor inhibition reduced prostasphere size and expression of PSA, but did not inhibit prostasphere formation. These effects are consistent with the androgen-independent self-renewal of cells with stem cell characteristics and the androgen-dependent proliferation of transit amplifying cells. As the canonical WNT signaling effector Β-catenin can also associate with the androgen receptor, we propose a model for tumour propagation involving a balance between WNT and androgen receptor activity. That would affect the self-renewal of a cancer cell with stem cell characteristics and drive transit amplifying cell proliferation and differentiation. In conclusion, we provide evidence that WNT activity regulates the self-renewal of prostate cancer cells with stem cell characteristics independently of androgen receptor activity. Inhibition of WNT signaling therefore has the potential to reduce the self-renewal of prostate cancer cells with stem cell characteristics and improve the therapeutic outcome.Peer reviewe

Comparative analysis of homology models of the Ah receptor ligand binding domain: Verification of structure-function predictions by site-directed mutagenesis of a nonfunctional receptor

The aryl hydrocarbon receptor (AHR) is a ligand-dependent transcription factor that mediates the biological and toxic effects of a wide variety of structurally diverse chemicals, including the toxic environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). While significant interspecies differences in AHR ligand binding specificity, selectivity, and response have been observed, the structural determinants responsible for those differences have not been determined, and homology models of the AHR ligand-binding domain (LBD) are available for only a few species. Here we describe the development and comparative analysis of homology models of the LBD of 16 AHRs from 12 mammalian and nonmammalian species and identify the specific residues contained within their ligand binding cavities. The ligand-binding cavity of the fish AHR exhibits differences from those of mammalian and avian AHRs, suggesting a slightly different TCDD binding mode. Comparison of the internal cavity in the LBD model of zebrafish (zf) AHR2, which binds TCDD with high affinity, to that of zfAHR1a, which does not bind TCDD, revealed that the latter has a dramatically shortened binding cavity due to the side chains of three residues (Tyr296, Thr386, and His388) that reduce the amount of internal space available to TCDD. Mutagenesis of two of these residues in zfAHR1a to those present in zfAHR2 (Y296H and T386A) restored the ability of zfAHR1a to bind TCDD and to exhibit TCDD-dependent binding to DNA. These results demonstrate the importance of these two amino acids and highlight the predictive potential of comparative analysis of homology models from diverse species. The availability of these AHR LBD homology models will facilitate in-depth comparative studies of AHR ligand binding and ligand-dependent AHR activation and provide a novel avenue for examining species-specific differences in AHR responsiveness. © 2013 American Chemical Society

Association of the tumor necrosis factor-alpha promoter polymorphism with change in triacylglycerol response to sequential meals

Background: Reported associations between Tumor Necrosis Factor-alpha (TNFA) and the postprandial triacylglycerol (TAG) response have been inconsistent, which could be due to variations in the TNFA gene, meal fat composition or participant’s body weight. Hence, we investigated the association of TNFA polymorphism (−308G → A) with body mass index (BMI) and postprandial lipaemia and also determined the impact of BMI on the association of the polymorphism with postprandial lipaemia. Methods: The study participants (n = 230) underwent a sequential meal postprandial study. Blood samples were taken at regular intervals after a test breakfast (t = 0, 49 g fat) and lunch (t =330 min, 29 g fat) to measure fasting and postprandial lipids, glucose and insulin. The Metabolic Challenge Study (MECHE) comprising 67 Irish participants who underwent a 54 g fat oral lipid tolerance test was used as a replication cohort. The impact of genotype on postprandial responses was determined using general linear model with adjustment for potential confounders. Results: The -308G → A polymorphism showed a significant association with BMI (P = 0.03) and fasting glucose (P = 0.006), where the polymorphism explained 13 % of the variation in the fasting glucose. A 30 % higher incremental area under the curve (IAUC) was observed for the postprandial TAG response in the GG homozygotes than A-allele carriers (P = 0.004) and the genotype explained 19 % of the variation in the IAUC. There was a non-significant trend in the impact of BMI on the association of the genotype with TAG IAUC (P = 0.09). These results were not statistically significant in the MECHE cohort, which could be due to the differences in the sample size, meal composition, baseline lipid profile, allelic diversity and postprandial characterisation of participants across the two cohorts. Conclusions: Our findings suggest that TNFA -308G → A polymorphism may be an important candidate for BMI, fasting glucose and postprandial TAG response. Further studies are required to investigate the mechanistic effects of the polymorphism on glucose and TAG metabolism, and determine whether BMI is an important variable which should be considered in the design of future studies. Trial registration: NCT01172951