Dataset of protein changes induced by cold acclimation in red clover (Trifolium pratense L.) populations recurrently selected for improved freezing tolerance

The data provide an overview of proteomic changes in red clover (Trifolium pratense L.) in response to cold acclimation and recurrent selection for superior freezing tolerance. Proteins were extracted from crowns of two red clover cultivars grown under non-acclimated or cold-acclimated conditions, and plants obtained from the initial genetic background (TF0) and from populations obtained after three (TF3) and four cycles (TF4) of recurrent selection for superior freezing tolerance. Proteins were analyzed using a two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled to mass spectroscopy (MS and MS/MS). Differentially regulated proteins were subsequently identified using MALDI TOF/TOF analysis. The data are related to a recently published research article describing proteome composition changes associated with freezing tolerance in red clover, “A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)” (Bertrand et al., 2016 [1]). They are available in the ProteomeXchange Consortium database via the PRIDE partner repository under the dataset identifier PRIDE: PXD003689. Keywords: Red clover, Cold acclimation, Proteomic analysis, Recurrent selection, Freezing tolerance, Plant abiotic stres

Improving salt stress responses of the symbiosis in alfalfa using salt-tolerant cultivar and rhizobial strain

Salt stress can affect alfalfa growth directly by adversely affecting metabolism, or indirectly by its effect on Rhizobium capacity for symbiotic N2 fixation. Growth and carbohydrate metabolism in leaves, roots and nodules of two alfalfa cultivars (Medicago sativa cv Apica and salt-tolerant cv Halo) in association with two rhizobial strains (A2 and salt-tolerant Rm1521) exposed to different levels of NaCl (0, 20, 40, 80 or 160 mM NaCl) were assessed under controlled conditions. For both cultivars, shoot and root biomasses and shoot to root ratio significantly declined with increasing NaCl concentrations. Under 80 mM NaCl, Halo plants yielded 20% more fresh shoot biomass than Apica while plants inoculated with Rm1521 allocated more biomass to the roots than to the shoots compared to A2. Halo plants maintained a steady shoot water content (about 80%) under the entire range of NaCl concentrations. Shoot water content was more variable in Apica. Apica in association with salt-tolerant strain Rm1521 maintained a better water status than with strain A2, as indicated by the higher shoot water content at 80 mM NaCl. Under salt stress, two major compatible sugars involved in plant osmoregulation, sucrose and pinitol, increased in leaves while a large accumulation of starch was observed in roots. In nodules, pinitol, sucrose and starch increased under salt stress and were much more abundant with strain Rm1521 than with A2. This suggests that there could be an active transport from the shoot to the nodules to help maintain nodule activity under NaCl stress and that strain Rm1521 increases the sink strength toward nodules. Our results show that combining cultivars and rhizobial strains with superior salt tolerance is an effective strategy to improve alfalfa productivity in salinity affected areasKeywords: Medicago sativa L. Salt stress Osmoregulation Pinitol Nodule Salt-tolerant cultiva

Selection of rhizobial strains differing in their nodulation kinetics under low temperature in four temperate legume species

Abstract Background Winter climate change including frequent freeze‐thaw episodes and shallow snow cover will have major impacts on the spring regrowth of perennial crops. Non‐bloating perennial forage legume species including sainfoin, birdsfoot trefoil, red clover, and alsike clover have been bred for their adaptation to harsh winter conditions. In parallel, the selection of cold‐tolerant rhizobial strains could allow earlier symbiotic nitrogen (N) fixation to hasten spring regrowth of legumes. Methods To identify strains forming nodules rapidly and showing high N‐fixing potential, 60 rhizobial strains in association with four temperate legume species were evaluated over 11 weeks under spring soil temperatures for kinetics of nodule formation, nitrogenase activity, and host yield. Results Strains differed in their capacity to form efficient nodules on legume hosts over time. Strains showing higher nitrogenase activity were arctic strain N10 with sainfoin and strain L2 with birdsfoot trefoil. For clovers, nitrogenase activity was similar for control and inoculated plants, likely due to formation of effective nodules in controls by endophyte rhizobia present in seeds. Conclusions Selection based on nodulation kinetics at low temperature, nitrogenase activity, and yield was effective to identify performant rhizobial strains for legume crops. The use of cold‐tolerant strains could help mitigate winter climatic changes

Development of hospice-palliative care with focus on Germany and the Czech Republic

Vývoj hospicové paliativní péče se zaměřením na Německo a Českou Republiku Development of hospice-palliative care with focus on Germany and the Czech Republic Ruth Kubíčková The aim of the thesis was to map the development, crucial moments and current .shape of hospice-palliative care and simultaneously confirm the thesis that the care of spiritual feature of dying persons has been an integral part of organized development of hospice care since its beginning and nowadays, it is still one of its principal themes. The first chapter deals with branch terminology since its clear definition is essential, not only for dialogue inside the system of palliative care providers and experts, but for interdisciplinary communication and cooperation which is - according to the definition of palliative care - the foundation of its holistic approach to patients. The second and third chapters are concerned with the development of nursing care since the ancient times to the diversification of modern hospice-palliative care at the end of the twentieth century. They deduce that the Christianity, with its central commandment to love thy neighbour as thyself, has changed the attitude to care of suffering persons, both in the nursing care and in shapes and ways of providing care. In the response to revolutionary changes in..

Additional file 3: Table S4a. of A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)

List of DIGE-spots with homology with sequences in databases that are up-regulated in response to cold acclimation (ANOVA, P < 0.01). (XLSX 27 kb

Additional file 4: Table S4b. of A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)

List of DIGE-spots with homology with sequences in databases that are down-regulated in response to cold acclimation (ANOVA, P < 0.01) (XLSX 18 kb