FranklinWORKS

It\u27s a fact of modern-day business life: Everyone is asked to do more with less. As a result, tough decisions are constantly in the works, including where to invest and where to save. Many organizations feel forced to focus on the negatives, such as cutbacks, reductions and sacrifices. However, research proves what you already know: People matter. Developing your workforce brings a valuable return on investment. With FranklinWORKS, partner organizations have access to a complete portfolio of educational options designed and delivered by a university that has been solely dedicated to educating ambitious students for more than 110 years. These scalable resources enable partners to recruit, train and retain the talent vital to moving their organizations forward.https://fuse.franklin.edu/ss2016/1020/thumbnail.jp

Review of the implications of changes in EU pesticides legislation on the production and export of fruits and vegetables from developing country suppliers

Since 1993 the European Union (EU) has been implementing a programme to establish harmonised Maximum Residue Levels (MRLs) for pesticide residues in foodstuffs sold in the EU. Between 1993 and July 2000, the EU has been aiming to establish MRLsfor 102 pesticide active ingredients. However, acceptable data for establishment of MRLs has not been available for a significant number of crop/active ingredient combinations. In such situations, the EU has left the MRLposition as an "openposition"for a limited period of time. During this period, data can be submitted to the EU to provide for the establishment of an MRL - this is usually done by agrochemical companies, but can also be done by other interested parties. If the period expires and no acceptable data has been received, the MRL is set at the analytical Limit of Determination (LOD) i.e. analytical zero

Spo0J and SMC are required for normal chromosome segregation in Staphylococcus aureus

Bacterial chromosome segregation is an essential cellular process that is particularly elusive in spherical bacteria such as the opportunistic human pathogen Staphylococcus aureus. In this study, we examined the functional significance of a ParB homologue, Spo0J, in staphylococcal chromosome segregation and investigated the role of the structural maintenance of chromosomes (SMC) bacterial condensin in this process. We show that neither spo0J nor smc is essential in S. aureus; however, their absence causes abnormal chromosome segregation. We demonstrate that formation of complexes containing Spo0J and SMC is required for efficient S. aureus chromosome segregation and that SMC localization is dependent on Spo0J. Furthermore, we found that cell division and cell cycle progression are unaffected by the absence of spo0J or smc. Our results verify the role of Spo0J and SMC in ensuring accurate staphylococcal chromosome segregation and also imply functional redundancy or the involvement of additional mechanisms that might contribute to faithful chromosome inheritance

Improve the performance of transfer learning without fine-tuning using dissimilarity-based multi-view learning for breast cancer histology images

Breast cancer is one of the most common types of cancer and leading cancer-related death causes for women. In the context of ICIAR 2018 Grand Challenge on Breast Cancer Histology Images, we compare one handcrafted feature extractor and five transfer learning feature extractors based on deep learning. We find out that the deep learning networks pretrained on ImageNet have better performance than the popular handcrafted features used for breast cancer histology images. The best feature extractor achieves an average accuracy of 79.30%. To improve the classification performance, a random forest dissimilarity based integration method is used to combine different feature groups together. When the five deep learning feature groups are combined, the average accuracy is improved to 82.90% (best accuracy 85.00%). When handcrafted features are combined with the five deep learning feature groups, the average accuracy is improved to 87.10% (best accuracy 93.00%)

Literacies

With the rise of new technologies and media, the way we communicate is rapidly changing. Literacies provides a comprehensive introduction to literacy pedagogy within today's new media environment. It focuses not only on reading and writing, but also on other modes of communication, including oral, visual, audio, gestural and spatial. This focus is designed to supplement, not replace, the enduringly important role of alphabetical literacy. Using real-world examples and illustrations, Literacies features the experiences of both teachers and students. It maps a range of methods that teachers can use to help their students develop their capacities to read, write and communicate. It also explores the wide range of literacies and the diversity of socio-cultural settings in today's workplace, public and community settings. With an emphasis on the 'how-to' practicalities of designing literacy learning experiences and assessing learner outcomes, this book is a contemporary and in-depth resource for literacy students

Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli

Understanding where proteins are localized in a bacterial cell is essential for understanding their function and regulation. This is particularly important for proteins that are involved in cell division, which localize at the division septum and assemble into highly regulated complexes. Current knowledge of these complexes has been greatly facilitated by super-resolution imaging using fluorescent protein fusions. Herein, we demonstrate with FtsZ that single-molecule PALM images can be obtained in-vivo using a genetically fused nanotag (ALFA), and a corresponding nanobody fused to mEos3.2. The methodology presented is applicable to other bacterial proteins.journal articl

Cost of stroke in Ontario, 1994/95

Abstract Background: Stroke is a major cause of death and disability in Ontario. This study estimates the cost of stroke, which can be used to help policy-makers appreciate the burden this disease places on society. Methods: A prevalence-based study measured disease-related costs in 1994/95 incurred by people with stroke. Direct costs included expenditures on hospital and other institutional care, services of physicians and other health professionals, drugs, research, home care, emergency health services, assistive devices and community support. Indirect costs were measured using the human-capital approach, which examines lost productivity due to premature death and disability. Results: A sensitivity analysis was used to estimate the total cost of stroke in Ontario to be $857 million, with a low of$719 and a high of $964 million. Direct costs were 60% of total costs. Stroke accounted for 3.9% of acute care hospital costs, 5.0% of rehabilitation, chronic care and other institutional costs, and 6.0% of home care costs. It accounted for a relatively minor proportion of physician costs (1.0%) and drug expenditures (0.3%). Interpretation: Stroke has a major economic impact in Ontario. Most direct costs of stroke are borne by the institutional health and community support sectors. These findings may provide guidance to policy-makers when they set priorities for research and prevention activities. Because we used provincial databases containing more detailed information than national databases, cost estimates in this study are more precise than national estimates. Because we used a strict definition of stroke, cost estimates may be considered conservative

Cell shape-independent FtsZ dynamics in synthetically remodeled bacterial cells

FtsZ is the main regulator of bacterial cell division. It has been implicated in acting as a scaffolding protein for other division proteins, a force generator during constriction, and more recently, as an active regulator of septal cell wall production. FtsZ assembles into a heterogeneous structure coined the Z-ring due to its resemblance to a ring confined by the midcell geometry. Here, to establish a framework for examining geometrical influences on proper Z-ring assembly and dynamics, we sculpted Escherichia coli cells into unnatural shapes using division- and cell wall-specific inhibitors in a micro-fabrication scheme. This approach allowed us to examine FtsZ behavior in engineered Z-squares and Z-hearts. We use stimulated emission depletion (STED) nanoscopy to show that FtsZ clusters in sculpted cells maintain the same dimensions as their wild-type counterparts. Based on our results, we propose that the underlying membrane geometry is not a deciding factor for FtsZ cluster maintenance and dynamics in vivo