Response of lettuce (Lactuca sativa L.) to salt stress

Lettuce is a highly nutritional crop that is sensitive to multiple stresses, including salt stress. The decreasing availability of salt-free water is posing a major challenge to growing nutritious lettuce in hydroponic systems. Despite evidence that salt stress affects yield and nutrition, its impacts on economically important growth stages are overlooked. This study addressed the knowledge gaps regarding the salt stress impact on romaine lettuce. Four independent experiments were conducted in controlled environment conditions using 2-38 lettuce genotypes, to assess the effects of salt stress at rosette or head formation. An increase in sodium chloride (NaCl) levels (0 to 150 mM) linearly decreased lettuce biomass. A positive association was observed between NaCl and sodium to potassium ion ratio. Lettuce was found to be sensitive to salt above 40 mM NaCl. The phenotyping of 38 lettuce genotypes revealed significant variability in salt stress tolerance. Genotype, Green forest exhibited a higher decline in fresh and dry biomass compared to Burgundy delight, indicating its higher sensitivity to salt stress. Sodium accumulation was significantly higher in both genotypes under salt stress, while potassium decreased in Green forest but remained relatively stable in Burgundy Delight. Hydro-primed lettuce had higher photosynthetic efficiency and oxidative stress tolerance than the control. Hydro-priming seed treatment could be used as a cost-effective priming technique to boost economic efficiency under stress. In addition, the low sodium: potassium ratio and high biomass can be used as indicators for selecting salt-tolerant genotypes

Citrus Essential Oils: A Suite of Insecticidal Compounds

Citrus essential oils (CEOs) and their constituent compounds are being reported to have multifarious activities. In this chapter an attempt is made to discuss the insecticidal activities, as well as CEO profile of different vegetative part of Citrus species and biocidal potentiality of their constituent compounds against diverse insect pests. It is observed that in most of the CEO constituent profile, limonene is the major constituent compound. Other important constituents present in different percentages in different CEOs are β-citronellal, linalool, pinene, β- caryophyllene, β-myrcene, terpinene, citral etc. These plant EO constituents are reported to have insecticidal effects against diverse insect species. Taking the four peel EOs of Citrus limon, Citrus paradisi, Citrus medica, Citrus maxima commonly grown in North Eastern part of India, study on their insecticidal effects against Dolichoderus affinis (Hymenoptera: Formicidae) was made and result is presented showing higher fumigant toxicity of C. medica and C. limon oil against the ant sp. With the increasing awareness for using safe insecticidal products among consumers, the citrus EOs with their attracting terpene compounds having good insecticidal potency bear all attributes to be used as commercial green pesticides in coming days both in indoor and outdoor management of insect pests

Building Capacities for Scaling-Up Climate Smart Village in Nepal: A training manual

Globally there has been enormous effort made by thousands of organizations to promote Climate Smart Agriculture (CSA) technologies for the sake of building resilience in agriculture and farmers’ livelihood. However, the progress so far is not satisfactory. Based on the past learnings CCAFS has conceptualized the idea of Climate Smart Village (CSV) which has CSA as the major component, along with other political and socio-economic dimensions. Looking at the successful piloting of CSV, the Government of Nepal has endorsed this approach in its program. However, a mechanism to share the idea of CSV to the development bodies is lacking. Similarly, the extension workers and other staffs of local governments do not have enough procedural understanding. Therefore, to scale-up CSV approach in Nepal, capacity building of government staffs and local leaders, especially at local and provincial level, is an absolute necessity. A guide book could be the means to facilitate the process of such capacity building. Hence this manual is developed as a guide book for organizing capacity building trainings to the government and non-government development professionals. It will also serve as a resource book to help development workers from local and provincial governments, local leaders, policy makers, researchers, and academicians to understand more about the idea and approach of CSV. Ultimately, this publication is expected to contribute to the mission of scaling-up the CSV approach for the enhanced resilience of farming communities in Nepal and around the world

Carbon footprint of Nepalese healthcare system: A study of Dhulikhel Hospital [version 1; peer review: 2 approved, 1 approved with reservations, 1 not approved]

Background: Though direct greenhouse gas emissions cannot be observed in health care sectors, there can exist indirect emissions contributing to global climate change. This study addresses the concept of the carbon footprint and its significance in understanding the environmental impact of human activities, with a specific emphasis on the healthcare sector through gate-to-gate (GtoG) life cycle assessment. Transportation, energy consumption, and solid waste generated by hospitals are the primary sources of carbon emissions. Methods: Different standards, guidelines and parameters were used to estimate emissions from both the primary and secondary data. All steps and sub-steps involved in GtoG were accessed and analyzed within the standard ISO 14040:44 guideline. An extensive review of existing literature was carried out for the evaluation and verification of secondary data. Results: The total carbon footprint of generators, electricity consumption, transportation activities, LPG cylinders, PV systems was found to be 58,780 kg-CO2-eq/yr, 519,794 kg-CO2-eq/yr, 272,375 kg-CO2-eq/yr, 44,494 kg-CO2-eq/yr, 35,283 kg-CO2-eq/yr respectively and the emissions from non-biodegradable solid waste was found to be 489,835 kg-CO2/yr. Local air pollutants such as PM10, CO, SO2, NOX, and VOCs generated by generators and transportation were also estimated. The CH4 emissions from liquid waste were 1177.344 kg CH4/BOD yr, and those from biodegradables were 3821.6954 kg CH4/yr. Conclusions: Healthcare professionals and policymakers can take action to reduce the sector's carbon footprint by implementing best practices and encouraging sustainable behavior. This study can be taken as foundation for further exploration of indirect emissions from healthcare sectors not only in Nepal but also in south Asian scenario

Evaluation of Phytochemical, Antioxidant and Antibacterial Activities of Selected Medicinal Plants

Medicinal plants are important reservoirs of bioactive compounds that need to be explored systematically. Because of their chemical diversity, natural products provide limitless possibilities for new drug discovery. This study aimed to investigate the biochemical properties of crude extracts from fifteen Nepalese medicinal plants. The total phenolic contents (TPC), total flavonoid contents (TFC), and antioxidant activity were evaluated through a colorimetric approach while the antibacterial activities were studied through the measurement of the zone of inhibition (ZoI) by agar well diffusion method along with minimum inhibitory concentrations (MIC) by broth dilution method. The methanolic extracts of Acacia catechu and Eupoterium adenophorum showed the highest TPC (55.21 ± 11.09 mg GAE/gm) and TFC (10.23 ± 1.07 mg QE/gm) among the studied plant extracts. Acacia catechu showed effective antioxidant properties with an IC50 value of 1.3 μg/mL, followed by extracts of Myrica esculenta, Syzygium cumini, and Mangifera indica. Morus australis exhibited antibacterial activity against Klebsiella pneumoniae (ZoI: 25mm, MIC: 0.012 mg/mL), Staphylococcus aureus ATCC 25923 (ZoI: 22 mm, MIC: 0.012 mg/mL), Pseudomonas aeruginosa (ZoI; 20 mm, MIC: 0.05 mg/mL), and methicillin-resistant Staphylococcus aureus (MRSA) (ZoI: 19 mm, MIC: 0.19 mg/mL). Morus australis extract showed a broad-spectrum antibacterial activity, followed by Eclipta prostrata, and Hypericum cordifolium. Future study is recommended to explore secondary metabolites of those medicinal plants to uncover further clinical efficacy

Digital learning Initiatives, Challenges and Achievement in Higher Education in Nepal Amidst COVID-19

The COVID-19 pandemic has affected higher education institutions (HEIs) worldwide and reshaped the existing educational system. Due to travel constraints and physical separation, there has been a global shift toward distance learning, and Nepal is no exception. This research intends to assess the practicality of online education by evaluating learners' experiences amidst COVID-19. A cross-sectional study was directed among HEIs students in Nepal using self-structured questionnaires. Our study revealed that 64.6% of the respondents were unsatisfied with online classes. More than half of the respondents (53.4%) use cell phones for online studies. Online education was reported to be unappealing to 28.8% of respondents. Variables such as age group (p = 0.05), enjoying class (p < 0.001), hours spent for an online class in a day (p = 0.05), and period for educational work using an electronic device (p = 0.1) were found significant with satisfaction level using both bivariate test and inferential test of univariate binary logistics regression. The challenges and opportunities encountered among students and faculties are highlighted along with the recommendations for fortifying communication in online-based teaching/learning

Monkeypox Outbreak: Wastewater and Environmental Surveillance Perspective

Monkeypox disease (MPXD), a viral disease caused by monkeypox virus (MPXV), is an emerging zoonotic disease endemic in some countries of Central and Western Africa but seldom reported outside the affected region. Since May 2022, MPXD has been reported at least in 74 countries globally, prompting the World Health Organization to declare the MPXD outbreak a Public Health Emergency of International Concern. As of July 24, 2022, 92% (68/74) of the countries with reported MPXD cases had no historical MPXD case reports. From the One Health perspective, the spread of MPXV in the environment poses a risk not only to humans but also to small mammals and may, ultimately, spread to potent novel host populations. Wastewater-based surveillance (WBS), has been extensively utilized for monitoring communicable diseases, particularly during the ongoing coronavirus disease, the COVID-19 pandemic It helped to monitor infectious disease caseloads as well as specific viral variants circulating in communities. The detection of MPXV DNA in various body fluids, including respiratory and nasal secretions, saliva, urine, feces, and semen of infected individuals, supports the possibility of using WBS as an early proxy for the detection of MPXV infections. WBS of MPXV DNA can be used to monitor MPXV activity/trends in sewerage network areas even before detecting laboratory-confirmed clinical cases within a community. However, several factors affect the detection of MPXV in wastewater including, but not limited to, routes and duration time of virus shedding by infected individuals, infection rates in the relevant affected population, environmental persistence, the processes and analytical sensitivity of the used methods. Further research is needed to identify the key factors that impact the detection of MPXV biomarkers in wastewater and improve the utility of WBS of MPXV as an early warning and monitoring tool for safeguarding human health. In this review, we shortly summarize aspects of MPXV outbreak relevant to wastewater monitoring and discuss the challenges associated with WBS.Peer reviewe

Determinants of agriculture biodiversity in Western Terai landscape complex of Nepal

The study explored agriculture biodiversity around protected areas and identified factors affecting diversity of agriculture biodiversity in farming households. The study analyzed the data collected from household survey of about 907 farmers from Western-Terai Landscape Complex of Nepal. Intra-species and inter-species richness and evenness in agriculture landscape were estimated and compared across a spectrum of land-uses. The study identified different social, economic, technological and ecological factors affecting the richness of intra-species and inter-species diversity of agriculture biodiversity using generalized linear regression models. Technology index, information index, food security, animal holding, ethnicity, irrigation facility and land-use were found as major variables affecting agriculture. The results also indicated that buffer zones had higher diversity than other land-uses, indicating positive effects of protected-land on surrounding agriculture biodiversity. Results supported need of coordinated efforts to mainstream agriculture biodiversity conservation with landscape conservation plans and socio-economic developments of the region

Determinants of agriculture biodiversity in Western Terai landscape complex of Nepal

The study explored agriculture biodiversity around protected areas and identified factors affecting diversity of agriculture biodiversity in farming households. The study analyzed the data collected from household survey of about 907 farmers from Western-Terai Landscape Complex of Nepal. Intra-species and inter-species richness and evenness in agriculture landscape were estimated and compared across a spectrum of land-uses. The study identified different social, economic, technological and ecological factors affecting the richness of intra-species and inter-species diversity of agriculture biodiversity using generalized linear regression models. Technology index, information index, food security, animal holding, ethnicity, irrigation facility and land-use were found as major variables affecting agriculture. The results also indicated that buffer zones had higher diversity than other land-uses, indicating positive effects of protected-land on surrounding agriculture biodiversity. Results supported need of coordinated efforts to mainstream agriculture biodiversity conservation with landscape conservation plans and socio-economic developments of the region