Microchip free-flow electrophoresis on glass substrate using laser-printing toner as structural material

In this work, a microfluidic free-flow electrophoresis device, obtained by thermal toner transferring on glass substrate, is presented. A microdevice can be manufactured in only 1 h. The layout of the microdevice was designed in order to improve the fluidic and electrical characteristics. The separation channel is 8 μm deep and presents an internal volume of 1.42 μL. The deleterious electrolysis effects were overcome by using a system that isolates the electrolysis products from the separation channel. The Joule heating dissipation in the separation channel was found to be very efficient up to a current density of 8.83 mA/mm2 that corresponds to a power dissipation per unit volume of running electrolyte of 172 mW/μL. Promising results were obtained in the evaluation of the microdevices for the separation of ionic dyes. The microfluidic device can be used for a continuous sample pretreatment step for micro total analysis system. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

Implementation of Safety Management of Selected Construction Companies in Manila

In the 1980s, the safety management system (SMS) was created to reduce material waste and the risk of accidents and fatalities in the construction industry. With the correct SMS deployment, construction companies can reduce safety-related difficulties.  This study assessed the current status of construction safety management intending to determine the level of implementation of selected construction companies with category AAA in Manila, Philippines. A survey was done to determine the SMS implementation level in terms of lack of control, basic cause, immediate cause, incident, and accident. The researcher used the quantitative method of research with the survey questionnaire as the main tool in gathering data. Architects, engineers, safety officers, and skilled workers were the respondents of the study. The gathered data were statistically treated with the use of Frequency, Percentage, Weighted Mean, Kruskal Wallis, Man Whitney U-test, and Spearman Rho Correlation. The overall results showed that the level of the selected construction companies in Manila is somewhat implemented. The results of this study are helpful to the industry as well because they can improve the understanding of SMS among industry professionals and aid in better SMS deployment at work

Microchip Free-flow Electrophoresis On Glass Substrate Using Laser-printing Toner As Structural Material

In this work, a microfluidic free-flow electrophoresis device, obtained by thermal toner transferring on glass substrate, is presented. A microdevice can be manufactured in only 1 h. The layout of the microdevice was designed in order to improve the fluidic and electrical characteristics. The separation channel is 8 μm deep and presents an internal volume of 1.42 μL. The deleterious electrolysis effects were overcome by using a system that isolates the electrolysis products from the separation channel. The Joule heating dissipation in the separation channel was found to be very efficient up to a current density of 8.83 mA/mm2 that corresponds to a power dissipation per unit volume of running electrolyte of 172 mW/μL. Promising results were obtained in the evaluation of the microdevices for the separation of ionic dyes. The microfluidic device can be used for a continuous sample pretreatment step for micro total analysis system. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.272449354942Manz, A., Graber, N., Widmer, H.M., (1990) Sens. Actuators, B1, pp. 244-248Reyes, D.R., Iossifidis, D., Auroux, P.-A., Manz, A., (2002) Anal. Chem., 74, pp. 2623-2636Reyes, D.R., Iossifidis, D., Auroux, P.-A., Manz, A., (2002) Anal. Chem., 74, pp. 2637-2652Lion, N., Rohner, T.C., Dayon, L., Arnaud, I.L., (2003) Electrophoresis, 24, pp. 3533-3562Barrolier, V.J., Watzke, E., Gibian, H., (1958) Z. Naturforsch., 13 B, pp. 754-755Hannig, K., (1961) Z. Anal. Chem., 181, pp. 244-254Wagner, H., (1989) Nature, 341, pp. 669-670Roman, M.C., Brown, P.R., (1994) Anal. Chem., 66, pp. 86A-94AKřivánková, L., Boček, P., (1998) Electrophoresis, 19, pp. 1064-1074Loseva, O.I., Gavryushkin, A.V., Osipov, V.V., Vanyakin, E.N., (1998) Electrophoresis, 19, pp. 1127-1134Poggel, M., Melin, T., (2001) Electrophoresis, 22, pp. 1008-1015Kašička, V., Prusik, Z., (1994) Am. Lab., 26, pp. 22-28Kašička, V., Prusik, Z., Sazelova, P., Jiracek, J., Barth, T., (1998) J. Chromatogr. A, 796, pp. 211-220Bauer, J., (1999) J. Chromatogr. B, 722, pp. 55-69Hirokawa, T., Ohta, T., Tanaka, I., Nakamura, K., (1993) J. Chromatogr., 638, pp. 215-223Chartogne, A., Tjaden, U.R., Van der Greef, J., (2000) Rapid Commun. Mass Spectrom., 14, pp. 1269-1274Schmitt-Kopplin, P., Kettrup, A., (2003) Electrophoresis, 24, pp. 3057-3066Mazereeuw, M., de Best, C.M., Tjaden, U.R., Irth, H., Van der Greef, J., (2000) Anal. Chem., 72, pp. 3881-3886Raymond, D.E., Manz, A., Widmer, H.M., (1994) Anal. Chem., 66, pp. 2858-2865Raymond, D.E., Manz, A., Widmer, H.M., (1996) Anal. Chem., 68, pp. 2515-2522Kobayashi, H., Shimamura, K., Akaida, T., Sakano, K., (2003) J. Chromatogr. A, 990, pp. 169-178Zhang, C.-X., Manz, A., (2003) Anal. Chem., 75, pp. 5759-5766Fonslow, B.R., Bowser, M.T., (2005) Anal. Chem., 77, pp. 5706-5710Macke, M., Andersson, P., Haddad, P.R., (1998) Anal. Chem., 70, pp. 743-749Lago, C.L., Silva, H.D.T., Neves, C.A., Brito-Neto, J.G.A., Fracassi da Silva, J.A., (2003) Anal. Chem., 75, pp. 3853-3858Lago, C.L., Neves, C.A., de Jesus, D.P., Silva, H.D.T., (2004) Electrophoresis, 25, pp. 3825-3831de Jesus, D.P., Brito-Neto, J.G.A., Richter, E.M., Angnes, L., (2005) Anal. Chem., 77, pp. 607-614Fracassi da Silva, J.A., Lago, C.L., (1998) Anal. Chem., 70, pp. 4339-434