Registration of retinal images from Public Health by minimising an error between vessels using an affine model with radial distortions

In order to estimate a registration model of eye fundus images made of an affinity and two radial distortions, we introduce an estimation criterion based on an error between the vessels. In [1], we estimated this model by minimising the error between characteristics points. In this paper, the detected vessels are selected using the circle and ellipse equations of the overlap area boundaries deduced from our model. Our method successfully registers 96 % of the 271 pairs in a Public Health dataset acquired mostly with different cameras. This is better than our previous method [1] and better than three other state-of-the-art methods. On a publicly available dataset, ours still better register the images than the reference method

Stress Corrosion Cracking of Stainless Steel Super Heater Tubes in 200 MW Boiler

A few cases of super heater tube failures by corrosion were noticed in a boiler of 210 MW units of NTPC stations. The failure investigation was carried out at the Research and Development centre of NTPC. The failed tubes were examined by using Optical, scanning Electron Microscope and conventional methods. The material of the tube was stabilised grade austenitic stainless steel. The micro-scopic examination indicated the presence of a large number of transgranular cracks with branches. The surface analysis by EDAX revealed the presence of chloride in the corrosion products. The reason of failure has been attributed to chloride induced stress corrosion cracking. Chloride ions had generated supposedly due to leaching of residual contaminants carried over from manufacturing stage. It was suggested to check the cracks already gene-rated by NDT methods and wash the tube panels which were already installed. After implementation of the recomm-endations, no such case of tube failure was reported. The present paper describes the investigations carried out and the remedial measures to prevent chloride induced stress corrosion cracking in supper heater tubes

Deciphering a Duality: Understanding Conflicting Standards in Sex & Violence Censorship in U.S. Obscenity Law

This research examines the division in US obscenity law that enables strict sex censorship while overlooking violence. By investigating the social and legal development of obscenity in US culture, I argue that the contemporary duality in obscenity censorship standards arose from a family of forces consisting of faith, economy, and identity in early American history. While sexuality ingrained itself in American culture as a commodity in need of regulation, violence was decentralized from the state and proliferated. This phenomenon led to a prioritization of suppressing sexual speech over violent speech. This paper traces the emergence this duality and its source

Clinical and bacteriological correlates of whole blood interferon gamma (IFN-γ) in newly detected cases of pulmonary TB

AbstractObjectiveTo determine the relationship of the capacity to produce interferon gamma (IFN-γ) in whole blood, bacteriological, hematological, radiographic and clinical presentations in new, HIV seronegative cases of pulmonary tuberculosis (TB).Methods80 cases and 50 control subjects aged 15 years onwards, representative of Kasturba Hospital and Nursing schools of Wardha district of Maharashtra state in India were examined for their health condition with standard methodology.ResultsAmong these TB patients, 73.8% were Quantiferon-TB gold (QFT) positive with IFN-γ concentration as 0.35 IU or more and there was none in healthy controls. The mean IFN-γ concentrations varied between 9.58 IU (50-59 yrs) and 2.58 IU (⩾60 yrs), showing no trend. The differences in positivity and mean IFN-γconcentrations were statistically insignificant. Both the QFT positivity and IFN-γconcentrations were higher in normal lymphocyte percent as compared to below and above normal, but differences were not statistically significant.ConclusionsThe IFN-γconcentrations are not correlated with any of the predictors of disease severity studied, the levels are significantly higher in observation group as compared to healthy group

Addition of a sequence from α2-antiplasmin transforms human serum albumin into a blood clot component that speeds clot lysis

<p>Abstract</p> <p>Background</p> <p>The plasma protein α₂-antiplasmin (α₂AP) is cross-linked to fibrin in blood clots by the transglutaminase factor XIIIa, and in that location retards clot lysis. Competition for this effect could be clinically useful in patients with thrombosis. We hypothesized that fusion of N-terminal portions of α₂-antiplasmin to human serum albumin (HSA) and production of the chimeric proteins in <it>Pichia pastoris </it>yeast would produce a stable and effective competitor protein.</p> <p>Results</p> <p>Fusion protein α₂AP(13-42)-HSA was efficiently secreted from transformed yeast and purified preparations contained within a mixed population the full-length intact form, while fusions with longer α₂AP moieties were inefficiently secreted and/or degraded. The α₂AP(13-42)-HSA protein, but not recombinant HSA, was cross-linked to both chemical lysine donors and fibrin or fibrinogen by factor XIIIa, although with less rapid kinetics than native α₂AP. Excess α₂AP(13-42)-HSA competed with α₂AP for cross-linking to chemical lysine donors more effectively than a synthetic α₂AP(13-42) peptide, and reduced the α₂AP-dependent resistance to fibrinolysis of plasma clots equally effectively as the peptide. Native α₂AP was found in <it>in vivo </it>clots in rabbits to a greater extent than α₂AP(13-42), however.</p> <p>Conclusion</p> <p>In this first report of transfer of transglutamination substrate status from one plasma protein to another, fusion protein α₂AP(13-42)-HSA was shown to satisfy initial requirements for a long-lasting, well-tolerated competitive inhibitor of α₂-antiplasmin predicted to act in a clot-localized manner.</p

Searching for pulsars associated with the Fermi GeV excess

The Fermi Large Area Telescope has detected an extended region of GeV emission towards the Galactic Centre that is currently thought to be powered by dark matter annihilation or a population of young and/or millisecond pulsars. In a test of the pulsar hypothesis, we have carried out an initial search of a 20 deg2 area centred on the peak of the galactic centre GeV excess. Candidate pulsars were identified as a compact, steep spectrum continuum radio source on interferometric images and followed with targeted single-dish pulsation searches. We report the discovery of the recycled pulsar PSR 1751−2737 with a spin period of 2.23 ms. PSR 1751−2737 appears to be an isolated recycled pulsar located within the disc of our Galaxy, and it is not part of the putative bulge population of pulsars that are thought to be responsible for the excess GeV emission. However, our initial success in this small pilot survey suggests that this hybrid method (i.e. wide-field interferometric imaging followed up with single-dish pulsation searches) may be an efficient alternative strategy for testing whether a putative bulge population of pulsars is responsible for the GeV excess

Season-wise length-weight relationship and relative condition factor of Tenualosa ilisha (Hamilton, 1822) at Narmada estuary, Gujarat, India

635-638Length-weight relationship and relative condition factor of T. ilisha was conducted for one year (2014-15) at Narmada estuary, Gujarat, India. Fish samples were collected from Bharuch, Bhadbhut, Mehgam and Ambetha landing sites covering 72 km of estuarine stretch. Two distinct fishing seasons were observed at Narmada estuary, viz., June-October and January-March, though the bulk of the harvest comes from monsoon season. A total of 312 fish samples were collected, 270 during monsoon and 42 during the winter season. The mean length and weight were recorded 35.37±11.36 cm and 625.83±39.81 g during monsoon and 36.84±3.82 cm and 537.44 ± 17.45 g during the winter season. The ‘b’ value was found to be 3.07 and 2.76 and relative condition factor was 1.02 and 1.01 during monsoon and winter seasons, respectively

New Spirometry Indices for Detecting Mild Airflow Obstruction.

The diagnosis of chronic obstructive pulmonary disease (COPD) relies on demonstration of airflow obstruction. Traditional spirometric indices miss a number of subjects with respiratory symptoms or structural lung disease on imaging. We hypothesized that utilizing all data points on the expiratory spirometry curves to assess their shape will improve detection of mild airflow obstruction and structural lung disease. We analyzed spirometry data of 8307 participants enrolled in the COPDGene study, and derived metrics of airflow obstruction based on the shape on the volume-time (Parameter D), and flow-volume curves (Transition Point and Transition Distance). We tested associations of these parameters with CT measures of lung disease, respiratory morbidity, and mortality using regression analyses. There were significant correlations between FEV1/FVC with Parameter D (r = -0.83; p &lt; 0.001), Transition Point (r = 0.69; p &lt; 0.001), and Transition Distance (r = 0.50; p &lt; 0.001). All metrics had significant associations with emphysema, small airway disease, dyspnea, and respiratory-quality of life (p &lt; 0.001). The highest quartile for Parameter D was independently associated with all-cause mortality (adjusted HR 3.22,95% CI 2.42-4.27; p &lt; 0.001) but a substantial number of participants in the highest quartile were categorized as GOLD 0 and 1 by traditional criteria (1.8% and 33.7%). Parameter D identified an additional 9.5% of participants with mild or non-recognized disease as abnormal with greater burden of structural lung disease compared with controls. The data points on the flow-volume and volume-time curves can be used to derive indices of airflow obstruction that identify additional subjects with disease who are deemed to be normal by traditional criteria

Comparison of Methods for the Purification of Alpha-1 Acid Glycoprotein from Human Plasma

Alpha-1 acid glycoprotein (AGP) is a highly glycosylated, negatively charged plasma protein suggested to have anti-inflammatory and/or immunomodulatory activities. Purification of AGP could be simplified if methods that exploit its high solubility under chemically harsh conditions could be demonstrated to leave the protein in its native conformation. Procedures involving exposure of AGP to hot phenol or sulphosalicylic acid (SSA) were compared to solely chromatographic methods. Hot phenol-purified AGP was more rapidly cleared from mice in vivo following intravenous injection than chromatographically purified AGP. In contrast, SSA-purified AGP demonstrated an identical in vivo clearance profile and circular dichroism spectrum to chromatographically purified AGP. Similarly, no differences in susceptibility to enzymatic deglycosylation or reactivity with Sambucus nigra lectin were detected between AGP purified via the two methods. Incorporation of the SSA step in the purification scheme for AGP eliminated the need for a large (4 mL resin/mL of plasma) initial chromatographic step and simplified its purification without causing any detectable distortion in the conformation of the protein. Confirmation that this procedure is nondenaturing will simplify AGP purification and investigation of its possible biological roles in laboratory animals

In Vivo Clearance of Alpha-1 Acid Glycoprotein Is Influenced by the Extent of Its N-Linked Glycosylation and by Its Interaction with the Vessel Wall

Alpha-1 acid glycoprotein (AGP) is a highly glycosylated plasma protein that exerts vasoprotective effects. We hypothesized that AGP's N-linked glycans govern its rate of clearance from the circulation, and followed the disappearance of different forms of radiolabeled human AGP from the plasma of rabbits and mice. Enzymatic deglycosylation of human plasma-derived AGP (pdAGP) by Peptide: N-Glycosidase F yielded a mixture of differentially deglycosylated forms (PNGase-AGP), while the introduction of five Asn to Gln mutations in recombinant Pichia pastoris-derived AGP (rAGP-N(5)Q) eliminated N-linked glycosylation. PNGase-AGP was cleared from the rabbit circulation 9-fold, and rAGP-N(5)Q, 46-fold more rapidly than pdAGP, primarily via a renal route. Pichia pastoris-derived wild-type rAGP differed from pdAGP in expressing mannose-terminated glycans, and, like neuraminidase-treated pdAGP, was more rapidly removed from the rabbit circulation than rAGP-N(5)Q. Systemic hyaluronidase treatment of mice transiently decreased pdAGP clearance. AGP administration to mice reduced vascular binding of hyaluronic acid binding protein in the liver microcirculation and increased its plasma levels. Our results support a critical role of N-linked glycosylation of AGP in regulating its in vivo clearance and an influence of a hyaluronidase-sensitive component of the vessel wall on its transendothelial passage