Detection sensitivity of laser feedback interferometry using a terahertz quantum cascade laser

We report on the high detection sensitivity of a laser feedback interferometry scheme based on a terahertz frequency quantum cascade laser. We show that variations on the laser voltage induced by optical feedback to the laser can be resolved with reinjection of powers as low as ~−125 dB of the emitted power. Our measurements demonstrate a noise equivalent power of ~1.4 pW/√Hz, although after accounting for reinjection losses we estimate this corresponds to only ~1 fW/√Hz being coupled to the quantum cascade laser active region

Origin of terminal voltage variations due to self-mixing in a terahertz frequency quantum cascade laser

The use of quantum cascade lasers (QCLs) for laser feedback interferometry (LFI) has received significant attention since it enables a wide range of sensing applications without requiring a separate detector, and hence simplifies experimental apparatus [1]. LFA (based on the self-mixing effect) refers to the partial reinjection of the radiation emitted from a laser after reflection from a target; the injected radiation field then interacts with the intra-cavity field causing measurable variations of the QCL terminal voltage. The theory of LFI with conventional laser sources is well studied and explained by the Lang–Kobayashi model [2, 3]. However, while this enables the dynamic state populations and light interaction to be modelled, a linear relationship between the change in cavity light power, ∆P, and terminal voltage variation is commonly assumed, i.e. VSM ∝ ∆P [4, 5]. This is not strictly applicable to QCL structures since carrier transport is dominated by the mechanisms of electron subband alignment, intersubband scattering and photon driven transport between subbands with energy separations that change with applied bias (terminal voltage). We present experimental results of a QCL which departs significantly from this assumed linear behavior. We observe strong enhancement of the self-mixing signal in regions where the local gradient of the current-voltage (I–V) curve increases. We explain the origin of this signal using an extended density matrix (DM) approach [6] which accounts for coherent transport and interaction of the optical light field with the active region. The model is used to calculate the I–V characteristics of a bound-to-continuum (BTC) terahertz (THz) QCL and predict the effect of light variation on terminal voltage at a fixed drive current. This approach is shown to predict the experimental signal with good agreement

Active phase-nulling of the self-mixing phase in a terahertz frequency quantum cascade laser

We demonstrate an active phase-nulling scheme for terahertz (THz) frequency quantum cascade lasers (QCLs) under optical feedback, by active electronic feedback control of the emission frequency. Using this scheme the frequency tuning rate of a THz QCL is characterised, with significantly reduced experimental complexity compared to alternative approaches. Furthermore, we demonstrate real-time displacement sensing of targets, overcoming the resolution limits imposed by quantisation in previously-implemented fringe counting methods. Our approach is readily applicable to high-frequency vibrometry and surface profiling of targets, as well as frequency-stabilisation schemes for THz QCLs

Interleukin-4 enhances proliferation of human pancreatic cancer cells: evidence for autocrine and paracrine actions

Interleukin-4 (IL-4) is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. Pancreatic cancer cells and tissues express high levels of IL-4 receptors. The aim of this study was to characterise the effects of IL-4 on the growth and signalling pathways of pancreatic cancer cells. Cell growth was determined by cell counting and MTT assays in association with fluorescence-activated cell sorter analysis, IL-4 expression using ELISA and real-time PCR techniques, and signal transduction using immunoprecipitation or immunoblot analysis. We now report for the first time that IL-4 significantly enhanced the growth of five out of six cultured pancreatic cancer cell lines in a dose-dependent manner in association with an increased fraction of cells in S-phase. Surprisingly, all six cell lines expressed endogenous IL-4, and IL-4 was detectable in the supernatant. Incubating cells with neutralising IL-4 antibodies resulted in a significant inhibition of basal growth in three cell lines, including IL-4-unresponsive MIA PaCa-2 cells, which however expressed the highest endogenous IL-4 levels. Interleukin-4 enhanced activity of MAPK, Akt-1, and Stat3 in IL-4-responsive, but not in IL-4-unresponsive MIA PaCa-2 cells; however, IL-4 enhanced tyrosine phosphorylation of insulin receptor substrate-1 and -2 in all cell lines. Our results demonstrate for the first time that pancreatic cancer cells produce IL-4 and that IL-4 can act as a growth factor in pancreatic cancer cells. Together with the observation that neutralising IL-4 antibodies can inhibit the growth of these cells, our results suggest that IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumour growth and metastasis

Terahertz imaging using quantum cascade lasers — a review of systems and applications

The terahertz (THz) frequency quantum cascade laser (QCL) is a compact source of THz radiation offering high power, high spectral purity and moderate tunability. As such, these sources are particularly suited to the application of THz frequency imaging across a range of disciplines, and have motivated significant research interest in this area over the past decade. In this paper we review the technological approaches to THz QCL-based imaging and the key advancements within this field. We discuss in detail a number of imaging approaches targeted to application areas including: multiple-frequency transmission and diffuse reflection imaging for the spectral mapping of targets; as well as coherent approaches based on the self-mixing phenomenon in THz QCLs for long-range imaging, three-dimensional imaging, materials analysis, and high-resolution inverse synthetic aperture radar imaging

The Actin-Binding Protein Capulet Genetically Interacts with the Microtubule Motor Kinesin to Maintain Neuronal Dendrite Homeostasis

BACKGROUND: Neurons require precise cytoskeletal regulation within neurites, containing microtubule tracks for cargo transport in axons and dendrites or within synapses containing organized actin. Due to the unique architecture and specialized function of neurons, neurons are particularly susceptible to perturbation of the cytoskeleton. Numerous actin-binding proteins help maintain proper cytoskeletal regulation. METHODOLOGY/PRINCIPAL FINDINGS: From a Drosophila forward genetic screen, we identified a mutation in capulet--encoding a conserved actin-binding protein--that causes abnormal aggregates of actin within dendrites. Through interaction studies, we demonstrate that simultaneous genetic inactivation of capulet and kinesin heavy chain, a microtubule motor protein, produces elongate cofilin-actin rods within dendrites but not axons. These rods resemble actin-rich structures induced in both mammalian neurodegenerative and Drosophila Alzheimer's models, but have not previously been identified by loss of function mutations in vivo. We further demonstrate that mitochondria, which are transported by Kinesin, have impaired distribution along dendrites in a capulet mutant. While Capulet and Cofilin may biochemically cooperate in certain circumstances, in neuronal dendrites they genetically antagonize each other. CONCLUSIONS/SIGNIFICANCE: The present study is the first molecularly defined loss of function demonstration of actin-cofilin rods in vivo. This study suggests that simultaneous, seemingly minor perturbations in neuronal dendrites can synergize producing severe abnormalities affecting actin, microtubules and mitochondria/energy availability in dendrites. Additionally, as >90% of Alzheimer's and Parkinson's cases are sporadic this study suggests mechanisms by which multiple mutations together may contribute to neurodegeneration instead of reliance on single mutations to produce disease

Overweight, physical activity, tobacco and alcohol consumption in a cross-sectional random sample of German adults

BACKGROUND: There is a current paucity of data on the health behaviour of non-selected populations in Central Europe. Data on health behaviour were collected as part of the EMIL study which investigated the prevalence of infection with Echinococcus multilocularis and other medical conditions in an urban German population. METHODS: Participating in the present study were 2,187 adults (1,138 females [52.0%]; 1,049 males [48.0%], age: 18–65 years) taken from a sample of 4,000 persons randomly chosen from an urban population. Data on health behaviour like physical activity, tobacco and alcohol consumption were obtained by means of a questionnaire, documentation of anthropometric data, abdominal ultrasound and blood specimens for assessment of chemical parameters. RESULTS: The overall rate of participation was 62.8%. Of these, 50.3% of the adults were overweight or obese. The proportion of active tobacco smokers stood at 30.1%. Of those surveyed 38.9% did not participate in any physical activity. Less than 2 hours of leisure time physical activity per week was associated with female sex, higher BMI (Body Mass Index), smoking and no alcohol consumption. Participants consumed on average 12 grams of alcohol per day. Total cholesterol was in 62.0% (>5.2 mmol/l) and triglycerides were elevated in 20.5% (≥ 2.3 mmol/l) of subjects studied. Hepatic steatosis was identified in 27.4% of subjects and showed an association with male sex, higher BMI, higher age, higher total blood cholesterol, lower HDL, higher triglycerides and higher ALT. CONCLUSION: This random sample of German urban adults was characterised by a high prevalence of overweight and obesity. This and the pattern of alcohol consumption, smoking and physical activity can be considered to put this group at high risk for associated morbidity and underscore the urgent need for preventive measures aimed at reducing the significantly increased health risk

Three-dimensional terahertz imaging using swept-frequency feedback interferometry with a quantum cascade laser

We demonstrate coherent three-dimensional terahertz imaging by frequency modulation of a quantum cascade laser in a compact and experimentally simple self-mixing scheme. Through this approach we can realize significantly faster acquisition rates compared to previous schemes employing longitudinal mechanical scanning of a sample. We achieve a depth resolution of better than 0.1 μm with a power noise spectral density below −50 dB/Hz, for a sampling time of 10 ms/pixel