Genetic association between sexual maturity and weekly live-weights in laying-type Japanese quail

This study focused on the estimation of genetic relationships between weekly live weights and sexual maturity of male and female Japanese quail. The live body weight data of a laying-type quail line over time were collected from hatching to six weeks of age. Sexual maturity was determined as the day of production of cloacal gland foam for males, and the day of laying the first egg for females. (Co)Variance components and genetic parameters were estimated, using MTDFREML, a restricted maximum likelihood (REML) procedure using a model with direct and maternal genetic effects. Heritability estimates of weekly live weights ranged from moderate to high (0.20 - 0.60), and were higher for males than for females. The variance due to maternal effects disappeared gradually for males but rapidly for females as the chicks grew older. Heritability of age of sexual maturation was 0.24 ± 0.008 and 033 ± 0.136 for males and females, respectively. Genetic correlations between ages of reaching sexual maturity and live weights (except hatchling weight) were favourable for males (-0.16 to –0.45) and unfavourable for females (0.14 to 0.22). Genetic correlations between males and females ranged from moderate (0.46 ± 0.083) to high (0.98 ± 0.139) for weekly live weights, and were 0.71 ± 0.24 for age of reaching sexual maturity. Sexual size dimorphism can be explained by the differences in pattern of heritability and correlation estimates between reaching sexual maturity and live weights for males and females. South African Journal of Animal Science Vol. 36(2) 2006: 142-14

DETERMINATION OF LANDSCAPE TYPOLOGY WITH REMOTE SENSING TECHNIQUES AND CORRELATION WITH DIFFERENT BIOCLIMATIC CONDITIONS: THE CASE OF BUYUK MENDERES BASIN

Due to the multidisciplinary nature of landscape research, there are different systems and methods for landscape identification and classification. The methodology of the study is based on landscape characterization, which is based on the pre-selection of geo-ecological and land-use related features of the landscape. For this purpose, landscape character types were identified using climate classes, land use/land cover (LULC), soil and morphological structure. In obtaining the LULC data, which is one of the major inputs of the study, 36-band images were created covering four seasons by producing NDVI (Normalized Difference Vegetation Index); LAI (Leaf Area Index); MSAVI2 (Modified Soil-Adjusted Vegetation Index); fCOVER (fraction of Cover); indices, which are important in determining LULC, together with the basebands in the remotely sensed Sentinel 2 satellite data. LAI is used to determine the amount of leaves per unit area; NDVI is used to determine the green areas in the area; fCOVER is used to determine the ratio of the study area to the area covered by vegetation, MSAVI2 is used to determine the amount of vegetation cover in the study area by using the soil reflectance correction factor (L). The phenological structure of the species was statistically determined with the indices derived from satellite images, and the bioclimatic condition and landscape character type were associated with the species

COVID-19 infection, vaccine status, and avoidance behaviors in adults with attention deficit and hyperactivity disorder: A cross-sectional study

ObjectiveWe aim to examine infection risk and vaccine status of COVID-19 in attention deficit and hyperactivity disorder and evaluate the impact of demographic, clinical, and COVID-19-related factors on the infection status and behavioral avoidance of COVID-19. MethodsThis cross-sectional study assessed adults with attention deficit and hyperactivity disorder recruited from an outpatient psychiatry clinic. Patients and healthy controls completed a survey on sociodemographic data, COVID-19 infection status, and vaccine status. COVID-19 Disease Perception Scale, COVID-19 Avoidance Attitudes Scale, Attitudes toward COVID-19 Vaccine Scale, Adult Attention Deficit and Hyperactivity Disorder Self-report Screening Scale for DSM-5, Adult Attention Deficit and Hyperactivity Disorder Self-Report Scale Symptoms Checklist, Patient Health Questionnaire-9, and State-Trait Anxiety Inventory were applied. ResultsNinety patients and 40 healthy controls participated. Patients did not differ from controls in COVID-19 infection and vaccine status, and behavioral avoidance of COVID-19. No demographic and clinical factor significantly affected the COVID-19 infection status. Patients scored higher than controls in the perception of COVID-19 as contagious (p = 0.038), cognitive avoidance of COVID-19 (p = 0.008), and positive attitudes toward the COVID-19 vaccine (p = 0.024). After adjustment of possible factors, a positive perception of the COVID-19 vaccine and a perception of COVID-19 as dangerous were the two factors significantly affecting behavioral avoidance of COVID-19 [R-2 = 0. 17, F(2) = 13.189, p < 0.0001]. ConclusionInfection and vaccine status of COVID-19 in patients did not significantly differ from controls. No demographic and clinical factor significantly affected the COVID-19 infection status. Approximately four-fifths of the patients were fully vaccinated as recommended by national and global health organizations. This has increased the knowledge base showing that the COVID-19 vaccine is acceptable and receiving the vaccine is endorsed by ADHD patients. Attention deficit and hyperactivity disorder itself may provoke no kind of mental disturbance in sense of perception of the danger of this disease. Our findings have increased the knowledge base showing that the COVID-19 vaccine is acceptable and the actual practice of receiving the vaccine is endorsed in this population. Our message for practice would be to take into account not only the core symptoms and the comorbidities of the disorder but also the perception of the disease while exploring its link with COVID-19

Spatial and temporal evaluation of soil erosion in Turkey under climate change scenarios using the Pan-European Soil Erosion Risk Assessment (PESERA) model

The impacts of climate change on soil erosion are mainly caused by the changes in the amount and intensity of rainfall and rising temperature. The combination of rainfall and temperature change is likely to be accompanied by negative or positive variations in agricultural and forest management. Turkey contains vast fertile plains, high mountain chains and semi-arid lands, with a climate that ranges from marine to continental and therefore is susceptible to soil erosion under climate change, particularly on high gradients and in semi-arid areas. This study aims to model the soil erosion risk under climate change scenarios in Turkey using the Pan-European Soil Erosion Assessment (PESERA) model, predicting the likely effects of land use/cover and climate change on sediment transport and soil erosion in the country. For this purpose, PESERA was applied to estimate the monthly and annual soil loss for 12 land use/cover types in Turkey. The model inputs included 128 variables derived from soil, climate, land use/cover and topography data. The total soil loss from the land surface is speculated to be approximately 285.5 million tonnes per year. According to the IPCC 5th Assessment Report of four climate change scenarios, the total soil losses were predicted as 308.9, 323.5, 320.3 and 355.3 million tonnes for RCP2.6, RCP4.5, RCP6.0 and RCP8.5 scenarios respectively from 2060 to 2080. The predicted amounts of fertile soil loss from agricultural land in a year were predicted to be 55.5 million tonnes at present, and 62.7, 59.9, 61.7 and 58.1 under RCP2.6, RCP4.5, RCP6.0 and RCP8.5 respectively. This confirms that approximately 30% of the total erosion occurs over the agricultural lands. In this respect, degraded forests, scrub and arable lands were subjected to the highest erosion rate (68%) of the total, whereas, fruit trees and berry plantations reflected the lowest erosion rates. Low soil organic carbon, sparse vegetation cover and variable climatic conditions significantly enhanced the erosion of the cultivated lands by primarily removing the potential food for organisms. Finally, process-based models offer a valuable resource for decision-makers when improving environmental management schemes and also decrease uncertainty when considering risks

Next-generation sequencing reveals deep intronic cryptic ABCC8 and HADH splicing founder mutations causing hyperinsulinism by pseudoexon activation

Copyright © 2013 The American Society of Human Genetics. Published by Elsevier Inc.Next-generation sequencing (NGS) enables analysis of the human genome on a scale previously unachievable by Sanger sequencing. Exome sequencing of the coding regions and conserved splice sites has been very successful in the identification of disease-causing mutations, and targeting of these regions has extended clinical diagnostic testing from analysis of fewer than ten genes per phenotype to more than 100. Noncoding mutations have been less extensively studied despite evidence from mRNA analysis for the existence of deep intronic mutations in >20 genes. We investigated individuals with hyperinsulinaemic hypoglycaemia and biochemical or genetic evidence to suggest noncoding mutations by using NGS to analyze the entire genomic regions of ABCC8 (117 kb) and HADH (94 kb) from overlapping ~10 kb PCR amplicons. Two deep intronic mutations, c.1333-1013A>G in ABCC8 and c.636+471G>T HADH, were identified. Both are predicted to create a cryptic splice donor site and an out-of-frame pseudoexon. Sequence analysis of mRNA from affected individuals' fibroblasts or lymphoblastoid cells confirmed mutant transcripts with pseudoexon inclusion and premature termination codons. Testing of additional individuals showed that these are founder mutations in the Irish and Turkish populations, accounting for 14% of focal hyperinsulinism cases and 32% of subjects with HADH mutations in our cohort. The identification of deep intronic mutations has previously focused on the detection of aberrant mRNA transcripts in a subset of disorders for which RNA is readily obtained from the target tissue or ectopically expressed at sufficient levels. Our approach of using NGS to analyze the entire genomic DNA sequence is applicable to any disease

One Step Nucleic Acid Amplification (OSNA) - a new method for lymph node staging in colorectal carcinomas

<p>Abstract</p> <p>Background</p> <p>Accurate histopathological evaluation of resected lymph nodes (LN) is essential for the reliable staging of colorectal carcinomas (CRC). With conventional sectioning and staining techniques usually only parts of the LN are examined which might lead to incorrect tumor staging. A molecular method called OSNA (One Step Nucleic Acid Amplification) may be suitable to determine the metastatic status of the complete LN and therefore improve staging.</p> <p>Methods</p> <p>OSNA is based on a short homogenisation step and subsequent automated amplification of cytokeratin 19 (CK19) mRNA directly from the sample lysate, with result available in 30-40 minutes. In this study 184 frozen LN from 184 patients with CRC were investigated by both OSNA and histology (Haematoxylin & Eosin staining and CK19 immunohistochemistry), with half of the LN used for each method. Samples with discordant results were further analysed by RT-PCR for CK19 and carcinoembryonic antigen (CEA).</p> <p>Results</p> <p>The concordance rate between histology and OSNA was 95.7%. Three LN were histology+/OSNA- and 5 LN histology-/OSNA+. RT-PCR supported the OSNA result in 3 discordant cases, suggesting that metastases were exclusively located in either the tissue analysed by OSNA or the tissue used for histology. If these samples were excluded the concordance was 97.2%, the sensitivity 94.9%, and the specificity 97.9%. Three patients (3%) staged as UICC I or II by routine histopathology were upstaged as LN positive by OSNA. One of these patients developed distant metastases (DMS) during follow up.</p> <p>Conclusion</p> <p>OSNA is a new and reliable method for molecular staging of lymphatic metastases in CRC and enables the examination of whole LN. It can be applied as a rapid diagnostic tool to estimate tumour involvement in LN during the staging of CRC.</p

European Industrial Relations: Transnational Relations and Global Challenges

This study investigates transnational relations and global challenges which the European Industrial Relations have been facing recently. The paper, methodologically, was structured with taking into account both socio- political and judicial arguments. The social theory, and ergo, the practice in Europe were analyzed according to Marxist point of view. Basically, industrial relations and employment relationship were examined from the perspectives of employees, employee representatives and nation-states. The influence of the Charter of Fundamental Rights which is legally binding with the Lisbon Treaty (TFEU) was examined. In addition, the effectiveness of the acquis communautaire within the EU was argued with respect to the European Social Model; such as, social dialogue, tripartite and bipartite information exchange and consultation, collective bargaining and legal provisions regarding employment conditions and social protection. The importance of Europeanisation and convergence of national industrial relations was illustrated. Keywords: Industrial Relations, Collective Bargaining, Europeanisatio

Systematic genetic testing for recessively inherited monogenic diabetes: a cross-sectional study in paediatric diabetes clinics

Data availability: The datasets supporting the current study have not been deposited in a public repository due to institutional ethics restrictions but are available from the corresponding author on request.This is the final version. Available from Springer via the DOI in this record. AIMS/HYPOTHESIS: Current clinical guidelines for childhood-onset monogenic diabetes outside infancy are mainly focused on identifying and testing for dominantly inherited, predominantly MODY genes. There are no systematic studies of the recessively inherited causes of monogenic diabetes that are likely to be more common in populations with high rates of consanguinity. We aimed to determine the contribution of recessive causes of monogenic diabetes in paediatric diabetes clinics and to identify clinical criteria by which to select individuals for recessive monogenic diabetes testing. METHODS: We conducted a cross-sectional study of 1093 children from seven paediatric diabetes clinics across Turkey (a population with high rates of consanguinity). We undertook genetic testing of 50 known dominant and recessive causes of monogenic diabetes for 236 children at low risk of type 1 diabetes. As a comparison, we used monogenic diabetes cases from UK paediatric diabetes clinics (a population with low rates of consanguinity). RESULTS: Thirty-four children in the Turkish cohort had monogenic diabetes, equating to a minimal prevalence of 3.1%, similar to that in the UK cohort (p = 0.40). Forty-one per cent (14/34) had autosomal recessive causes in contrast to 1.6% (2/122) in the UK monogenic diabetes cohort (p 10%) assisted the identification of the dominant (all p ≤ 0.0003) but not recessive cases (all p ≥ 0.2) in Turkey. The presence of certain non-autoimmune extra-pancreatic features greatly assisted the identification of recessive (p < 0.0001, OR 66.9) but not dominant cases. CONCLUSIONS/INTERPRETATION: Recessively inherited mutations are a common cause of monogenic diabetes in populations with high rates of consanguinity. Present MODY-focused genetic testing strategies do not identify affected individuals. To detect all cases of monogenic paediatric diabetes, it is crucial that recessive genes are included in genetic panels and that children are selected for testing if they have certain non-autoimmune extra-pancreatic features in addition to current criteria.Wellcome TrustRoyal SocietyNational Institute for Health Researc