Multiparametric Semi-quantitative Scoring System for the histological evaluation of marine fish larval and juvenile quality

Gilthead seabream (GSB - Sparus aurata) and European seabass (ESB - Dicentrarchus labrax) are two of the most farmed fish species in EU. However, production of sea bream/bass in the EU has remained stagnant for the last decade and the Mediterranean EU aquaculture faces significant sustainability challenges. In consideration of this, and as it is largely recognized that the success of marine aquaculture strictly depends on the production of good quality larvae/juveniles, in this paper the authors put forward an original standardized tool for the histological assessment of GSB and ESB larva/juveniles. This tool promptly allows to highlight problems in marine fish larval batches because of managerial practices, suggesting to fish farmers which direction take to resolve them. A Multiparametric Semi-quantitative Scoring System (scoring range 1–5) has been originally developed for larval/juvenile histological evaluation and it includes 18 descriptors related to 6 organ districts. The values of each descriptor can be summarized in two indexes: the CHI (Cumulative Histological Index), giving general information about the quality of a fish batch in that precise moment and the OCV (Organ condition value) showing the general condition of each organ and by the individual descriptors. The paper purposes are to describe the MSSS, the criteria established for the score attribution and to supply some indications for the use of the tool

Intestinal morpho-physiology and innate immune status of European sea bass (Dicentrarchus labrax) in response to diets including a blend of two marine microalgae, Tisochrysis lutea and Tetraselmis suecica

The aim of this study was to investigate the effects of replacing graded levels of dietary fish meal by a blend of two marine microalgae Tisochrysis lutea and Tetraselmis suecica on intestinal morpho-physiology and innate immune response in European sea bass. Two complete diets were formulated to be iso-nitrogenous and isolipidic and prepared by including a blend of the two microalgae, to replace approximately 15 and 45% fish meal protein of the control diet. A fourth diet, where the microalgae mix was substituted by soybean meal, was also prepared. Each diet was offered until visual satiety over 105 days to triplicated groups of European sea bass (204 \ub1 12.7 g), kept in a recirculating marine water system. The humoral and cellular innate immune parameters of E. sea bass were affected by the dietary treatment. Fish fed the microalgae-containing or the soybean rich diets, showed a significantly greater villi height, while the thickness of intestinal epithelium was significantly reduced in fish fed the soybean meal-rich diet. The activity of the brush border membrane enzymes, maltase, sucrase-isomaltase, \u3b3-glutamil transferase and alkaline phosphatase was not affected by dietary treatment but changed in different intestinal tracts. The genes sucrase-isomaltase, peptide transporter 1, sodium/potassium-transporting ATPase and aminopeptidase N were overexpressed in the pyloric and proximal region of the intestine of fish fed the microalgae-including diets. In conclusion, a blend of dried marine microalgae Tisochrysis lutea and Tetraselmis suecica as alternative ingredients to dietary fish meal did not hamper gut digestive-absorptive functions of E. sea bass. Moreover, it resulted in enhanced non-specific immune response, suggesting an effective role as an immunostimulant ingredien

Geometric properties of galactic discs with clumpy episodes

A scenario for the formation of the bi-modality in the chemical space [α/Fe] vs [Fe/H] of the Milky Way was recently proposed in which α-enhanced stars are produced early and quickly in clumps. Besides accelerating the enrichment of the medium with α-elements, these clumps scatter the old stars, converting in-plane to vertical motion, forming a geometric thick disc. In this paper, by means of a detailed analysis of the data from smooth particle hydrodynamical simulations, we investigate the geometric properties (in particular of the chemical thick disc) produced in this scenario. For mono-age populations we show that the surface radial density profiles of high-[α/Fe] stars are well described by single exponentials, while that of low-[α/Fe] stars require broken exponentials. This break is sharp for young populations and broadens for older ones. The position of the break does not depend significantly on age. The vertical density profiles of mono-age populations are characterized by single exponentials, which flare significantly for low-[α/Fe] stars but only weakly (or not at all) for high-[α/Fe] stars. For low-[α/Fe] stars, the flaring level decreases with age, while for high-[α/Fe] stars it weakly increases with age (although with large uncertainties). All these properties are in agreement with observational results recently reported for the Milky Way, making this a plausible scenario for the formation of the Galactic thick disc

Box/peanut-shaped bulges in action space

Abstract We introduce the study of box/peanut (B/P) bulges in the action space of the initial axisymmetric system. We explore where populations with different actions end up once a bar forms and a B/P bulge develops. We find that the density bimodality due to the B/P bulge (the X-shape) is better traced by populations with low radial, $\rm J_{R,0}$, or vertical, $\rm J_{z,0}$, actions, or high azimuthal action, $\rm J_{\phi ,0}$. Generally populations separated by $\rm J_{R,0}$ have a greater variation in bar strength and vertical heating than those separated by $\rm J_{z,0}$. While the bar substantially weakens the initial vertical gradient of $\rm J_{z,0}$, it also drives a strikingly monotonic vertical profile of $\rm J_{R,0}$. We then use these results to guide us in assigning metallicity to star particles in a pure N-body model. Because stellar metallicity in unbarred galaxies depends on age as well as radial and vertical positions, the initial actions are particularly well suited for assigning metallicities. We argue that assigning metallicities based on single actions, or on positions, results in metallicity distributions inconsistent with those observed in real galaxies. We therefore use all three actions to assign metallicity to an N-body model by comparing with the actions of a star-forming, unbarred simulation. The resulting metallicity distribution is pinched on the vertical axis, has a realistic vertical gradient and has a stronger X-shape in metal-rich populations, as found in real galaxies

Comparative Therapeutic Effects of Natural Compounds Against Saprolegnia spp. (Oomycota) and Amyloodinium ocellatum (Dinophyceae)

The fish parasites Saprolegnia spp. (Oomycota) and Amyloodinium ocellatum (Dinophyceae) cause important losses in freshwater and marine aquaculture industry, respectively. The possible adverse effects of compounds used to control these parasites in aquaculture resulted in increased interest on the search for natural products with antiparasitic activity. In this work, eighteen plant-derived compounds (2′,4′-Dihydroxychalcone; 7-Hydroxyflavone; Artemisinin; Camphor (1R); Diallyl sulfide; Esculetin; Eucalyptol; Garlicin 80%; Harmalol hydrochloride dihydrate; Palmatine chloride; Piperine; Plumbagin; Resveratrol; Rosmarinic acid; Sclareolide; Tomatine, Umbelliferone, and Usnic Acid) have been tested in vitro. Sixteen of these were used to determine their effects on the gill cell line G1B (ATCC®CRL-2536™) and on the motility of viable dinospores of Amyloodinium ocellatum, and thirteen were screened for inhibitory activity against Saprolegnia spp. The cytotoxicity results on G1B cells determined that only two compounds (2′,4′-Dihydroxychalcone and Tomatine) exhibited dose-dependent toxic effects. The highest surveyed concentrations (0.1 and 0.01 mM) reduced cell viability by 80%. Upon lowering the compound concentration the percentage of dead cells was lower than 20%. The same two compounds revealed to be potential antiparasitics by reducing in a dose-dependent manner the motility of A. ocellatum dinospores up to 100%. With respect to Saprolegnia, a Minimum Inhibitory Concentration was found for Tomatine (0.1 mM), Piperine and Plumbagin (0.25 mM), while 2′,4′-Dihydroxychalcone considerably slowed down mycelial growth for 24 h at a concentration of 0.1 mM. Therefore, this research allowed to identify two compounds, Tomatine and 2′,4′-Dihydroxychalcone, effective against both parasites. These compounds could represent promising candidates for the treatment of amyloodiniosis and saprolegniosis in aquaculture. Nevertheless, further in vitro and in vivo tests are required in order to determine concentrations that are effective against the considered pathogens but at the same time safe for hosts, environment and consumers

Bending waves excited by irregular gas inflow along warps

Gaia has revealed clear evidence of bending waves in the vertical kinematics of stars in the solar neighbourhood. We study bending waves in two simulations, one warped, with the warp due to misaligned gas inflow, and the other unwarped. We find slow, retrograde bending waves in both models, with the ones in the warped model having larger amplitudes. We also find fast, prograde bending waves. Prograde bending waves in the unwarped model are very weak, in agreement with the expectation that these waves should decay on short, approximately crossing, time-scales, due to strong winding. However, prograde bending waves are much stronger for the duration of the warped model, pointing to irregular gas inflow along the warp as a continuous source of excitation. We demonstrate that large-amplitude bending waves that propagate through the solar neighbourhood give rise to a correlation between the mean vertical velocity and the angular momentum, with a slope consistent with that found by Gaia. The bending waves affect populations of all ages, but the sharpest features are found in the young populations, hinting that short-wavelength waves are not supported by the older, kinematically hotter, populations. Our results demonstrate the importance of misaligned gas accretion as a recurrent source of vertical perturbations of disc galaxies, including in the Milky Way

Transcriptome analysis of amyloodinium ocellatum tomonts revealed basic information on the major potential virulence factors

The ectoparasite protozoan Amyloodinium ocellatum (AO) is the etiological agent of amyloodiniosis in European seabass (Dicentrarchus labrax) (ESB). There is a lack of information about basic molecular data on AO biology and its interaction with the host. Therefore, de novo transcriptome sequencing of AO tomonts was performed. AO trophonts were detached from infested ESB gills, and quickly becoming early tomonts were purified by Percoll\uae density gradient. Tomont total RNA was processed and quality was assessed immediately. cDNA libraries were constructed using TruSeq\uae Stranded mRNA kit and sequenced using Illumina sequencer. CLC assembly was used to generate the Transcriptome assembly of AO tomonts. Out of 48,188 contigs, 56.12% belong to dinophyceae wherein Symbiodinium microadriaticum had 94.61% similarity among dinophyceae. Functional annotations of contigs indicated that 12,677 had associated GO term, 9005 with KEGG term. The contigs belonging to dinophyceae resulted in the detection of several peptidases. A BLAST search for known virulent factors from the virulence database resulted in hits to Rab proteins, AP120, Ribosomal phosphoprotein, Heat-shock protein70, Casein kinases, Plasmepsin IV, and Brucipain. Hsp70 and casein kinase II alpha were characterized in-silico. Altogether, these results provide a reference database in understanding AO molecular biology, aiding to the development of novel diagnostics and future vaccines

GASTRO library I: the simulated chemodynamical properties of several GSE-like stellar halos

The Milky Way stellar halo contains relics of ancient mergers that tell the story of our Galaxy's formation. Some of them are identified due to their similarity in energy, actions and chemistry, referred to as the "chemodynamical space", and are often attributed to distinct merger events. It is also known that our Galaxy went through a significant merger event that shaped the local stellar halo during its first Gyr. Previous studies using $N$-body only and cosmological hydrodynamical simulations have shown that such single massive merger can produce several "signatures" in the chemodynamical space, which can potentially be misinterpreted as distinct merger events. Motivated by these, in this work we use a subset of the GASTRO, library which consists of several SPH+$N$-body models of single accretion event in a Milky Way-like galaxy. Here, we study models with orbital properties similar to the main merger event of our Galaxy and explore the implications to known stellar halo substructures. We find that: $i.$ supernova feedback efficiency influences the satellite's structure and orbital evolution, resulting in distinct chemodynamical features for models with the same initial conditions, $ii.$ very retrograde high energy stars are the most metal-poor of the accreted dwarf galaxy and could be misinterpreted as a distinct merger $iii.$ the most bound stars are more metal-rich in our models, the opposite of what is observed in the Milky Way, suggesting a secondary massive merger, and finally $iv.$ our models can reconcile other known substructures to an unique progenitor.Comment: Published in Ap