Suppression of properties associated with malignancy in murine melanoma-melanocyte hybrid cells.

Murine and human melanoma cells differ relatively reliably from non-tumorigenic melanocytes in certain biological properties. When cultured at low pH, melanocytes tend to be pigmented and melanoma cells unpigmented. The growth of virtually all metastatic melanoma cells is inhibited by phorbol esters such as TPA (12-O-tetradecanoyl phorbol-13-acetate), which stimulate melanocyte growth. Melanocytes fail to grow in suspension culture or produce tumours when implanted in animals, while many melanoma lines can do both. Here we studied which of these properties were dominant in hybrid cells formed by fusion of drug-resistant murine B16-F10RR melanoma cells to melanocytes of the albino and brown lines, melan-c and melan-b. The albino melanocytes are unpigmented but well-differentiated, the brown melanocytes produce pale brown pigment and the melanoma cells are unpigmented under the conditions used. All hybrid colonies observed produced black pigment, except some melan-b/melanoma hybrids when growing sparsely with TPA. Thus pigmentation was generally dominant. 14/15 hybrid lines showed stimulation of proliferation by TPA, as do melanocytes. Most hybrid lines showed no or reduced capacity for growth in suspension, though some grew better in suspension when TPA was present. There was marked suppression of the tumorigenicity of the parental melanoma cells in 4/8 hybrids examined, and tumorigenicity was reduced in the others, despite considerable chromosome loss by the passage level tested. Thus most properties of the non-tumorigenic pigment cells were dominant, as often observed for other cell lineages, and providing further evidence for gene loss in the genesis of malignant melanoma

Mapping the CMB I: the first flight of the QMAP experiment

We report on the first flight of the balloon-borne QMAP experiment. The experiment is designed to make a map of the cosmic microwave background anisotropy on angular scales from 0.7 to several degrees. Using the map we determine the angular power spectrum of the anisotropy in multipole bands from l~40 to l~140. The results are consistent with the Saskatoon (SK) measurements. The frequency spectral index (measured at low l) is consistent with that of CMB and inconsistent with either Galactic synchrotron or free-free emission. The instrument, measurement, analysis of the angular power spectrum, and possible systematic errors are discussed.Comment: 4 pages, with 5 figures included. Submitted to ApJL. Window functions and color figures are available at http://pupgg.princeton.edu/~cmb/welcome.htm

Optical Design of the Atacama Cosmology Telescope and the Millimeter Bolometric Array Camera

The Atacama Cosmology Telescope is a 6-meter telescope designed to map the Cosmic Microwave Background simultaneously at 145 GHz, 215 GHz, and 280 GHz with arcminute resolution. Each frequency will have a 32 by 32 element focal plane array of TES bolometers. This paper describes the design of the telescope and the cold reimaging optics, which is optimized for millimeter-wave observations with these sensitive detectors.Comment: 23 pages. Accepted for publication in Applied Optics. Several minor clarifications added after peer revie

Topological Defects in an Open Universe

(To appear in Nuclear Physics B Supplements Proceedings section) This talk will explore the evolution of topological defects in an open universe. The rapid expansion of the universe in an open model slows defects and suppresses the generation of CBR fluctuations at large angular scale as does the altered relationship between angle and length in an open universe. Defect models, when normalized to COBE in an open universe, predict a galaxy power spectrum consistent with the galaxy power spectrum inferred from the galaxy surveys and do not require an extreme bias. Neither defect models in a flat universe nor standard inflationary models can fit either the multipole spectrum or the power spectrum inferred from galaxy surveys.Comment: 11 pages and 4 figures, Elsevier Publisher's LaTeX, POP-54

On the formation of glycolaldehyde in dense molecular cores

Glycolaldehyde is a simple monosaccharide sugar linked to prebiotic chemistry. Recently, it was detected in a molecular core in the star-forming region G31.41+0.31 at a reasonably high abundance. We investigate the formation of glycolaldehyde at 10 K to determine whether it can form efficiently under typical dense core conditions. Using an astrochemical model, we test five different reaction mechanisms that have been proposed in the astrophysical literature, finding that a gas-phase formation route is unlikely. Of the grain-surface formation routes, only two are efficient enough at very low temperatures to produce sufficient glycolaldehyde to match the observational estimates, with the mechanism culminating in CH3OH + HCO being favored. However, when we consider the feasibility of these mechanisms from a reaction chemistry perspective, the second grain-surface route looks more promising, H3CO + HCO

A Measurement of the Angular Power Spectrum of the CMB from l = 100 to 400

We report on a measurement of the angular spectrum of the CMB between $l\approx 100$ and $l\approx 400$ made at 144 GHz from Cerro Toco in the Chilean altiplano. When the new data are combined with previous data at 30 and 40 GHz, taken with the same instrument observing the same section of sky, we find: 1) a rise in the angular spectrum to a maximum with $\delta T_l \approx 85~\mu$K at $l\approx 200$ and a fall at $l>300$, thereby localizing the peak near $l\approx 200$; and 2) that the anisotropy at $l\approx 200$ has the spectrum of the CMB.Comment: 4 pages, 2 figures. Revised version; includes Ned Wright's postscript fix. Accepted by ApJL. Website at http://physics.princeton.edu/~cmb

Retention and intracellular distribution of instilled iron oxide particles in human alveolar macrophages

Bronchoalveolar lavage (BAL) was used to sample retention of particles within the alveolar macrophage (AM) compartment at various times from 1 to 91 d following intrapulmonary instillation of 2.6-μm-diameter iron oxide (Fe2O3) particles in human subjects. Particles were cleared from the lavagable AM compartment in a biphasic pattern, with a rapid-phase clearance half-time of 0.5 d and long-term clearance half-time of 110 d, comparable to retention kinetics determined by more traditional methods. The intracellular distribution of particles within lavaged AMs was similar in bronchial and alveolar BAL fractions. AMs with high intracellular particle burdens disappeared from the lavagable phagocytic AM population disproportionately more rapidly (shorter clearance half-time) than did AMs with lower particle burdens, consistent with the occurrence of a particle redistribution phenomenon as previously described in similar studies in rats. The rates of AM disappearance from the various particle burden categories was generally slightly slower in bronchial fractions than in alveolar fractions. The instillation of particles induced a transient acute inflammatory response at 24 h postinstillation (PI), characterized by increased numbers of neutrophils and alveolar macrophages in BAL fluids. This response was subclinical and was resolved within 4 d PI

Reputation Agent: Prompting Fair Reviews in Gig Markets

Our study presents a new tool, Reputation Agent, to promote fairer reviews from requesters (employers or customers) on gig markets. Unfair reviews, created when requesters consider factors outside of a worker's control, are known to plague gig workers and can result in lost job opportunities and even termination from the marketplace. Our tool leverages machine learning to implement an intelligent interface that: (1) uses deep learning to automatically detect when an individual has included unfair factors into her review (factors outside the worker's control per the policies of the market); and (2) prompts the individual to reconsider her review if she has incorporated unfair factors. To study the effectiveness of Reputation Agent, we conducted a controlled experiment over different gig markets. Our experiment illustrates that across markets, Reputation Agent, in contrast with traditional approaches, motivates requesters to review gig workers' performance more fairly. We discuss how tools that bring more transparency to employers about the policies of a gig market can help build empathy thus resulting in reasoned discussions around potential injustices towards workers generated by these interfaces. Our vision is that with tools that promote truth and transparency we can bring fairer treatment to gig workers.Comment: 12 pages, 5 figures, The Web Conference 2020, ACM WWW 202

Continuity or change in business representation in Britain? An assessment of the Heseltine initiatives of the 1990s

Britain has a fragmented, overlapping, and underresourced system of business representation. Attempts at reform, however, have proved difficult and largely unsuccessful. A coherent and logical system is relevant, in terms of both an effective dialogue between government and business, and the promotion of competitiveness and productivity. Through interviews and archival evidence, I look at how government has attempted to reform business associations. The main focus is the Heseltine initiatives of the 1990s: I outline the various initiatives taken, reveal the extent to which policy represented continuity or change, and consider whether the initiatives were effective. I show that they had a degree of success but that they would have made greater impact if they had been sustained over a longer period of time. A consideration of the historical context, moreover, suggests there may be limits to the role of government intervention in business association reform

The authority of moral oversight : on the legitimacy of criminal law

An influential view in recent philosophy of punishment is that the apparatus of criminal justice should be geared at least in part to state censure of wrongdoing. I argue that if it were to be so geared, such an apparatus would make ambitious claims to authority, and that the legitimacy of the relevant state would then depend on whether those claims can be vindicated. This paper looks first at what kind of authority is being claimed by this apparatus. The criminal law, I argue, cannot merely be thought of as claiming a right to rule and to be obeyed. Rather, its authority is better understood as the authority of moral oversight: a power to alter, at will (though within certain limits), citizens’ liability to answer for their compliance with—and to be officially censured for their failure to comply with—a designated set of pre-existing moral reasons. The paper then looks at whether a state could realistically be expected to possess such authority—that is, whether a state that claims to have such a power could ever be legitimate