Starbursts versus Truncated Star Formation in Nearby Clusters of Galaxies

We present long-slit spectroscopy, B and R bandpass imaging, and 21 cm observations of a sample of early-type galaxies in nearby clusters which are known to be either in a star-forming phase or to have had star formation which recently terminated. From the long-slit spectra, obtained with the Blanco 4-m telescope, we find that emission lines in the star-forming cluster galaxies are significantly more centrally concentrated than in a sample of field galaxies. The broadband imaging reveals that two currently star-forming early-type galaxies in the Pegasus I cluster have blue nuclei, again indicating that recent star formation has been concentrated. In contrast, the two galaxies for which star formation has already ended show no central color gradient. The Pegasus I galaxy with the most evident signs of ongoing star formation (NGC7648), exhibits signatures of a tidal encounter. Neutral hydrogen observations of that galaxy with the Arecibo radiotelescope reveal the presence of ~4 x 10^8 solar masses of HI. Arecibo observations of other current or recent star-forming early-type galaxies in Pegasus I indicate smaller amounts of gas in one of them, and only upper limits in others.Comment: to be published in Astronomical Journa

Ultrasonic Plate Wave Evaluation of Natural Fiber Composite Panels

Two key shortcomings of current ultrasonic nondestructive evaluation (NDE) techniques for plywood, medium density fiberboard (MDF), and oriented strandboard are the reliance on empirical correlations and the neglect of valuable waveform information. The research reported herein examined the feasibility of using fundamental mechanics, wave propagation, and laminated, shear deformable plate theories to nondestructively evaluate material properties in natural fiber-based composite panels. Dispersion curves were constructed exhibiting the variation of flexural plate wave phase velocity with frequency. Based on shear deformable laminated plate wave theory, flexural and transverse shear rigidity values for solid transversely isotropic, laminated transversely isotropic, and solid orthotropic natural fiber-based composite panels were obtained from the dispersion curves. Axial rigidity values were obtained directly from extensional plate wave phase velocity. Excellent agreement (within 3%) of flexural rigidity values was obtained between NDE and mechanical testing for most panels. Transverse shear modulus values obtained from plate wave tests were within 4% of values obtained from through-thickness ultrasonic shear wave speed. Tensile and compressive axial rigidity values obtained from NDE were 22% to 41% higher than mechanical tension and compression test results. These differences between NDE and axial mechanical testing results are likely due to load-rate effects; however, these large differences were not apparent in the flexural and transverse shear comparisons. This fundamental research advances the state-of-the-art of NDE of wood-based composites by replacing empirical approaches with a technique based on fundamental mechanics, shear deformation laminated plate theory, and plate wave propagation theory

Differential Binding of Co(II) and Zn(II) to Metallo-β-Lactamase Bla2 from \u3cem\u3eBacillus anthracis\u3c/em\u3e

In an effort to probe the structure, mechanism, and biochemical properties of metallo-β-lactamase Bla2 from Bacillus anthracis, the enzyme was overexpressed, purified, and characterized. Metal analyses demonstrated that recombinant Bla2 tightly binds 1 equiv of Zn(II). Steady-state kinetic studies showed that mono-Zn(II) Bla2 (1Zn-Bla2) is active, while di-Zn(II) Bla2 (ZnZn-Bla2) was unstable. Catalytically, 1Zn-Bla2 behaves like the related enzymes CcrA and L1. In contrast, di-Co(II) Bla2 (CoCo-Bla2) is substantially more active than the mono-Co(II) analogue. Rapid kinetics and UV−vis, 1H NMR, EPR, and EXAFS spectroscopic studies show that Co(II) binding to Bla2 is distributed, while EXAFS shows that Zn(II) binding is sequential. To our knowledge, this is the first documented example of a Zn enzyme that binds Co(II) and Zn(II) via distinct mechanisms, underscoring the need to demonstrate transferability when extrapolating results on Co(II)-substituted proteins to the native Zn(II)-containing forms

Halo Structures of Gravitational Lens Galaxies

We explore the halo structure of four gravitational lenses with well-observed, thin Einstein rings. We find that the gravitational potentials are well described by ellipsoidal density distributions in the sense that the best-fit nonellipsoidal models have parameters consistent with their ellipsoidal counterparts. We find upper limits on the standard parameters for the deviation from an ellipse of |a_3/a_0|<0.023, 0.019, 0.037, and 0.035, and |a_4/a_0|<0.034, 0.041, 0.051, and 0.064 for SDSS J0924+0219, HE0435-1223, B1938+666, and PG1115+080, respectively. We find that the lens galaxies are at the centers of their dark matter halos, and obtain upper limits for the offset of each center of mass from the center of light of |Delta x|<0.004, 0.005, 0.009, and 0.005 arcsec, corresponding to 22, 29, 70, and 23 pc. These limits also exclude the possibility of any significant lopsidednessof the dark matter halos and set an upper limit of f_sat<sqrt(N)% on the mass fraction of massive substructures inside the Einstein ring if they are divided over N satellites. We also explore the properties of galaxies as substructures in groups for the lens PG1115+080, finding evidence for dark matter halos associated with the galaxies but no evidence for a clear distinction between satellite and central galaxies.Comment: 10 pages, 5 figures, accepted for publication in The Astrophysical Journa

Policy Recommendations for Meeting the Grand Challenge to Ensure Healthy Development for All Youth

This brief was created forSocial Innovation for America’s Renewal, a policy conference organized by the Center for Social Development in collaboration with the American Academy of Social Work & Social Welfare, which is leading theGrand Challenges for Social Work initiative to champion social progress. The conference site includes links to speeches, presentations, and a full list of the policy briefs

Progress in Interferometry for LISA at JPL

Recent advances at JPL in experimentation and design for LISA interferometry include the demonstration of Time Delay Interferometry using electronically separated end stations, a new arm-locking design with improved gain and stability, and progress in flight readiness of digital and analog electronics for phase measurements.Comment: 11 pages, 9 figures, LISA 8 Symposium, Stanford University, 201

The structure of Herpesvirus Fusion Glycoprotein B-Bilayer Complex reveals the protein-membrane and lateral protein-protein interaction

Glycoprotein B (gB) is a key component of the complex herpesvirus fusion machinery. We studied membrane interaction of two gB ectodomain forms and present an electron cryotomography structure of the gB-bilayer complex. The two forms differed in presence or absence of the membrane proximal region (MPR) but showed an overall similar trimeric shape. The presence of the MPR impeded interaction with liposomes. In contrast, the MPR-lacking form interacted efficiently with liposomes. Lateral interaction resulted in coat formation on the membranes. The structure revealed that interaction of gB with membranes was mediated by the fusion loops and limited to the outer membrane leaflet. The observed intrinsic propensity of gB to cluster on membranes indicates an additional role of gB in driving the fusion process forward beyond the transient fusion pore opening and subsequently leading to fusion pore expansion

TOM40 Mediates Mitochondrial Dysfunction Induced by α-Synuclein Accumulation in Parkinson's Disease.

Alpha-synuclein (α-Syn) accumulation/aggregation and mitochondrial dysfunction play prominent roles in the pathology of Parkinson's disease. We have previously shown that postmortem human dopaminergic neurons from PD brains accumulate high levels of mitochondrial DNA (mtDNA) deletions. We now addressed the question, whether alterations in a component of the mitochondrial import machinery -TOM40- might contribute to the mitochondrial dysfunction and damage in PD. For this purpose, we studied levels of TOM40, mtDNA deletions, oxidative damage, energy production, and complexes of the respiratory chain in brain homogenates as well as in single neurons, using laser-capture-microdissection in transgenic mice overexpressing human wildtype α-Syn. Additionally, we used lentivirus-mediated stereotactic delivery of a component of this import machinery into mouse brain as a novel therapeutic strategy. We report here that TOM40 is significantly reduced in the brain of PD patients and in α-Syn transgenic mice. TOM40 deficits were associated with increased mtDNA deletions and oxidative DNA damage, and with decreased energy production and altered levels of complex I proteins in α-Syn transgenic mice. Lentiviral-mediated overexpression of Tom40 in α-Syn-transgenic mice brains ameliorated energy deficits as well as oxidative burden. Our results suggest that alterations in the mitochondrial protein transport machinery might contribute to mitochondrial impairment in α-Synucleinopathies

Using Genomic Variation to Distinguish Ovarian High-Grade Serous Carcinoma from Benign Fallopian Tubes

The preoperative diagnosis of pelvic masses has been elusive to date. Methods for characterization such as CA-125 have had limited specificity. We hypothesize that genomic variation can be used to create prediction models which accurately distinguish high grade serous ovarian cancer (HGSC) from benign tissue. Methods: In this retrospective, pilot study, we extracted DNA and RNA from HGSC specimens and from benign fallopian tubes. Then, we performed whole exome sequencing and RNA sequencing, and identified single nucleotide variants (SNV), copy number variants (CNV) and structural variants (SV). We used these variants to create prediction models to distinguish cancer from benign tissue. The models were then validated in independent datasets and with a machine learning platform. Results: The prediction model with SNV had an AUC of 1.00 (95% CI 1.00-1.00). The models with CNV and SV had AUC of 0.87 and 0.73, respectively. Validated models also had excellent performances. Conclusions: Genomic variation of HGSC can be used to create prediction models which accurately discriminate cancer from benign tissue. Further refining of these models (early-stage samples, other tumor types) has the potential to lead to detection of ovarian cancer in blood with cell free DNA, even in early stage

Less-than-lethal "flashbang" diversionary device.

Diversionary devices such as flashbang grenades are used in a wide variety of military and law-enforcement operations. They function to distract and/or incapacitate adversaries in scenarios ranging from hostage rescue to covert strategic paralysis operations. There are a number of disadvantages associated with currently available diversionary devices. Serious injuries and fatalities have resulted from their use both operationally and in training. Because safety is of paramount importance, desired improvements to these devices include protection against inadvertent initiation, the elimination of the production of high-velocity fragments, less damaging decibel output and increased light output. Sandia National Laboratories has developed a next-generation diversionary flash-bang device that will provide the end user with these enhanced safety features