292 research outputs found
PENGARUH FEAR OF MISSING OUT (FOMO) TERHADAP KEPUASAN HIDUP GENERASI Z SELAMA PANDEMI COVID-19
Fear of Missing Out (FoMO) merupakan rasa takut atau cemas jika seseorang ketinggalan sesuatu yang menarik akibat tidak memenuhi kebutuhan dasar psikologis. Belakangan ini, FoMO dicatatkan meningkat selama pandemi, khususnya pada Generasi Z. FoMO juga dinyatakan dapat mengubah kepuasan hidup seseorang. Penelitian ini bertujuan untuk mengetahui apakah terdapat pengaruh FoMO terhadap kepuasan hidup generasi Z selama pandemi COVID-19. Sampel penelitian ini adalah 221 generasi Z yang berusia 18-25 tahun. Penelitian ini menggunakan skala Fear of Missing Out Scale (Przybylski et al., 2013) dan Satisfaction with Life Scale (Diener et al., 1985). Hasil penelitian menunjukkan terdapat pengaruh FoMO terhadap kepuasan hidup generasi Z yang berarah negatif, yaitu sebanyak -0,119, yang berarti jika FoMO meningkat, maka kepuasan hidup akan menurun. Hasil uji regresi sederhana menunjukkan bahwa FoMO berpengaruh sebanyak 4,7% terhadap kepuasan hidup generasi Z selama pandemi COVID-19.
Fear of Missing Out (FoMO) is an anxious feeling when one is missing out on something interesting due to the lack of psychological needs. As of lately, FoMO has surged during the pandemic, especially amongst generation Z. FoMO is also stated to be related to one’s life satisfaction. The aim of this research is to explore the effects of FoMO on generation Z’s life satisfaction during COVID-19. This research has collected data from 221 respondents, aging from 18-25. Scales used in this research are Fear of Missing Out Scales (Przybylski et al., 2013) and Satisfaction with Life Scales (Diener et al., 1985). The results prove that FoMO has a negative effect on Gen-Z’s life satisfaction during COVID-19 (b = -0,119). Linear regression shows that FoMO has an effect of 4,7% on Gen-Z’s life satisfaction during the pandemic
Constitutive expression of E- and P-selectin cognate ligands in human endothelial cells.
On human endothelial cells from umbilical cord (HUVEC) are present, in addition to E- and P-selectins, their cognate ligands. Differently from selectins, the ligand expression is constitutive and not modulated by interleukin-1beta. Such ligands appear to be different from the ones present in promyelocytic cells in order to promote cell adhesion to immobilized selectins. The expression of selectin-ligands on HUVEC cells suggest that selectins can participate in endothelial signalling besides their role as adhesion molecules for circulating blood cells. However, despite their role in chemotaxis, selectins do not contribute to HUVEC tube formation in Matrigel
The cytotoxic effect of unconjugated bilirubin in human neuroblastoma SH-SY5Y cells is modulated by the expression level of MRP1 but not MDR1
In vitro and in vivo studies have demonstrated that UCB (unconjugated
bilirubin) is neurotoxic. Although previous studies suggested
that both MRP1 (multidrug resistance-associated protein
1) and MDR1 (multidrug resistance protein 1) may protect cells
against accumulation of UCB, direct comparison of their role in
UCB transport was never performed. To this end, we used an inducible
siRNA (small interfering RNA) expression system to silence
the expression of MRP1 and MDR1 in human neuroblastoma
SH-SY5Y cells. The effects of in vitro exposure to clinicallyrelevant
levels of unbound UCB were compared between
unsilenced (control) cells and cells with similar reductions in the
expression of MRP1 or MDR1, documented by RT\u2013PCR (reverse
transcription\u2013PCR) (mRNA), immunoblotting (protein), and for
MDR1, the enhanced net uptake of a specific fluorescent substrate.
Cytotoxicity was assessed by the MTT [3-(4,5-dimethylthiazol-2-
yl)-2,5-diphenyl-2H-tetrazolium bromide] test. MRP1-deficient
cells accumulated significantly more UCB and suffered greater
cytotoxicity than controls. By contrast, MDR1-deficient cells
exhibited UCB uptake and cytotoxicity comparable with controls.
At intermediate levels of silencing, the increased susceptibility to
UCB toxicity closely correlated with the decrease in the expression
of MRP1, but not of MDR1. These data support the concept
that limitation of cellular UCB accumulation, due to UCB export
mediated by MRP1, but not MDR1, plays an important role in
preventing bilirubin encephalopathy in the newborn
Multiple genome relationships and a complex biogeographic history in the eastern range of Quercus suber L. (Fagaceae) implied by nuclear and chloroplast DNA variation
The complex evolutionary history of Quercus suber has been throughly investigated in many recent works, but the details of its differentiation processes are still largely unknown. In addition, the geographical and evolutionary roles of the eastern parts of the species range have gained much less attention compared to other southern European areas. In order to fill this gap, new insights to infer the species diversification and range establishment of the cork oak in the east-central Mediterranean are here provided by means of inter- and intra-specific plastid DNA and nuclear ribosomal ITS phylogeographic studies. We analyzed 95 natural cork oak populations; 6 closely related, sympatric oaks were included in the study and used for comparisons.Evidence for a clear phylogeographical structure was detected with PCR-RFLP at 5 chloroplast loci, while ITS sequence variation is apparently unrelated with the geographical distribution. Five chloroplast haplotypes and three ITS main lineages were identified. Three haplotypes and all ITS lineages occur in the Italian Peninsula, stressing the importance of these territories for the evolutionary history of the species. Two divergent "Italian" haplotypes are highly shared, and one ITS variant is basal to the ingroup, revealing sister relationships within Cerris taxonomic group. Hypotheses of hybridization, lineage sorting of ancient DNA polymorphisms and of reticulate evolution of the whole species group are presented and discussed
Garden in motion. An experience of citizens involvement in public space regeneration
The paper illustrates a Placemaking process developed in Potenza Municipality (Southern Italy), based on an interpretation of the theories by the French landscape architect Gilles Clément. A laboratory has been organized in a residual area of the city, famous for an architectural monument, the bridge designed by Sergio Musmeci. The Internet allows a continuous online storytelling of work, creating citizens engagement on projects or choices and producing creativity and knowledge circulation. In this perspective "Garden in Motion" initiative produced new important processes for the community life, just like in Gilles Clément's "Garden in motion", where the processes of nature are favoured and spontaneous plants put in condition to grow and move freely
A transcriptome analysis identifies molecular effectors of unconjugated bilirubin in human neuroblastoma SH-SY5Y cells
Background: The deposition of unconjugated bilirubin (UCB) in selected regions of the brain results in irreversible neuronal damage, or Bilirubin Encephalopathy (BE). Although UCB impairs a large number of cellular functions in other tissues, the basic mechanisms of neurotoxicity have not yet been fully clarified. While cells can accumulate UCB by passive diffusion, cell protection may involve multiple mechanisms including the extrusion of the pigment as well as pro-survival homeostatic responses that are still unknown.
Results: Transcriptome changes induced by UCB exposure in SH-SY5Y neuroblastoma cell line were examined by high density oligonucleotide microarrays. Two-hundred and thirty genes were induced after 24 hours. A Gene Ontology (GO) analysis showed that at least 50 genes were directly involved in the endoplasmic reticulum (ER) stress response. Validation of selected ER stress genes is shown by quantitative RT-PCR. Analysis of XBP1 splicing and DDIT3/CHOP subcellular localization is presented.
Conclusion: These results show for the first time that UCB exposure induces ER stress response as major intracellular homeostasis in surviving neuroblastoma cells in vitro
Aluminium to Carbon Fibre Reinforced Polymer tubes joints produced by magnetic pulse welding
Project SpIM, (2016/10605). ESA (ESA contract 4000111471/14/NL/PA). UID/EMS/00667/2019 (UNIDEMI).In this work, Aluminium AA7075-T6 to Carbon Fibre Reinforced Polymer (CFRP) tube joints using magnetic pulse welding technology were performed. The process was found suitable for joining this material combination when a rigid support is used to constrain the CFRP wall displacement. A minimum discharge energy of 2.5 kJ was seen to produce tight interfaces in tubular geometry for 1 mm of stand-off distance. A larger number of turns in the coil was found to reduce both the damages caused by the impact forces and to increase the joint axial resistance. To protect the CFRP tube from impacts and achieve a weld, an electrolytic Nickel coating was deposited over the CFRP tube. Welding was produced between the coating and the aluminium tube with no intermetallic compounds observed on the interface for the energies tested.authorsversionpublishe
3,3′-(1,4-Phenylene)bis[2-(propylamino)benzofuro[3,2-d]pyrimidin-4(3H)-one] ethanol disolvate
The title compound, C32H28N6O4·2C2H5OH, consists of two 2-(propylamino)benzofuro[3,2-d]pyrimidin-4(3H)-one units connected, via one of the pyrimidine N atoms, to a bridging benzene ring in the 1,4 positions. Two ethanol solvent molecules are also present. The main molecule lies on a center of symmetry located at the center of the benzene ring. The fused-ring system of the benzofuro[3,2-d]pyrimidine moiety is nearly planar (r.m.s. deviation = 0.016 Å) and forms a dihedral angle of 78.21 (7)° with the central benzene ring. The crystal structure features O—H⋯O and N—H⋯O interactions. The C atoms of the propylamino side chain in the main molecule and the ethyl group in the solvent molecule are disordered over two positions, with site-occupancy factors 0.34:0.66 and 0.42:0.58, respectively
Diplomatic Villa
Zadaním práce je návrh rezidencie pre diplomatické účely. Stavba sa nachádza v lokalite Troja, ktorá bola spracovaná v súčasne v ateliéri.The assignment was to design a residence with diplomatic purpose. Building is located in Troja, which was elaborated in studio
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