Parental Motivation in Family Farm Intergenerational Transfers

An intergenerational transfer model incorporating both altruism and exchange is presented for family farm transfers. A simulation study is conducted to test parental motivation in intergenerational transfers of family farm businesses. Results indicated that family farm intergenerational transfers are altruistically motivated.Family Farms, Intergenerational Transfer, Family Farm Succession, Consumer/Household Economics, Farm Management, Q10, Q12,

Microminiaturized, biopotential conditioning system (MBCS)

Multichannel, medical monitoring system allows almost complete freedom of movement for subject during monitoring periods. System comprises monitoring unit (biobelt), transmission line, and data acquisition unit. Belt, made of polybenzimidizole fabric, is wrapped around individual's waist and held in place by overlapping sections of Velcro closure material

A fully (3+1)-D Regge calculus model of the Kasner cosmology

We describe the first discrete-time 4-dimensional numerical application of Regge calculus. The spacetime is represented as a complex of 4-dimensional simplices, and the geometry interior to each 4-simplex is flat Minkowski spacetime. This simplicial spacetime is constructed so as to be foliated with a one parameter family of spacelike hypersurfaces built of tetrahedra. We implement a novel two-surface initial-data prescription for Regge calculus, and provide the first fully 4-dimensional application of an implicit decoupled evolution scheme (the ``Sorkin evolution scheme''). We benchmark this code on the Kasner cosmology --- a cosmology which embodies generic features of the collapse of many cosmological models. We (1) reproduce the continuum solution with a fractional error in the 3-volume of 10^{-5} after 10000 evolution steps, (2) demonstrate stable evolution, (3) preserve the standard deviation of spatial homogeneity to less than 10^{-10} and (4) explicitly display the existence of diffeomorphism freedom in Regge calculus. We also present the second-order convergence properties of the solution to the continuum.Comment: 22 pages, 5 eps figures, LaTeX. Updated and expanded versio

Interacting vector fields in Relativity without Relativity

Barbour, Foster and \'{O} Murchadha have recently developed a new framework, called here {\it{the 3-space approach}}, for the formulation of classical bosonic dynamics. Neither time nor a locally Minkowskian structure of spacetime are presupposed. Both arise as emergent features of the world from geodesic-type dynamics on a space of 3-dimensional metric--matter configurations. In fact gravity, the universal light cone and Abelian gauge theory minimally coupled to gravity all arise naturally through a single common mechanism. It yields relativity -- and more -- without presupposing relativity. This paper completes the recovery of the presently known bosonic sector within the 3-space approach. We show, for a rather general ansatz, that 3-vector fields can interact among themselves only as Yang--Mills fields minimally coupled to gravity.Comment: Replaced with final version accepted by Classical and Quantum Gravity (14 pages, no figures

Proof of the Thin Sandwich Conjecture

We prove that the Thin Sandwich Conjecture in general relativity is valid, provided that the data $(g_{ab},\dot g_{ab})$ satisfy certain geometric conditions. These conditions define an open set in the class of possible data, but are not generically satisfied. The implications for the ``superspace'' picture of the Einstein evolution equations are discussed.Comment: 8 page

Scale-Invariant Gravity: Geometrodynamics

We present a scale-invariant theory, conformal gravity, which closely resembles the geometrodynamical formulation of general relativity (GR). While previous attempts to create scale-invariant theories of gravity have been based on Weyl's idea of a compensating field, our direct approach dispenses with this and is built by extension of the method of best matching w.r.t scaling developed in the parallel particle dynamics paper by one of the authors. In spatially-compact GR, there is an infinity of degrees of freedom that describe the shape of 3-space which interact with a single volume degree of freedom. In conformal gravity, the shape degrees of freedom remain, but the volume is no longer a dynamical variable. Further theories and formulations related to GR and conformal gravity are presented. Conformal gravity is successfully coupled to scalars and the gauge fields of nature. It should describe the solar system observations as well as GR does, but its cosmology and quantization will be completely different.Comment: 33 pages. Published version (has very minor style changes due to changes in companion paper

Global Regulatory Functions of the Staphylococcus aureus Endoribonuclease III in Gene Expression

RNA turnover plays an important role in both virulence and adaptation to stress in the Gram-positive human pathogen Staphylococcus aureus. However, the molecular players and mechanisms involved in these processes are poorly understood. Here, we explored the functions of S. aureus endoribonuclease III (RNase III), a member of the ubiquitous family of double-strand-specific endoribonucleases. To define genomic transcripts that are bound and processed by RNase III, we performed deep sequencing on cDNA libraries generated from RNAs that were co-immunoprecipitated with wild-type RNase III or two different cleavage-defective mutant variants in vivo. Several newly identified RNase III targets were validated by independent experimental methods. We identified various classes of structured RNAs as RNase III substrates and demonstrated that this enzyme is involved in the maturation of rRNAs and tRNAs, regulates the turnover of mRNAs and non-coding RNAs, and autoregulates its synthesis by cleaving within the coding region of its own mRNA. Moreover, we identified a positive effect of RNase III on protein synthesis based on novel mechanisms. RNase III–mediated cleavage in the 5′ untranslated region (5′UTR) enhanced the stability and translation of cspA mRNA, which encodes the major cold-shock protein. Furthermore, RNase III cleaved overlapping 5′UTRs of divergently transcribed genes to generate leaderless mRNAs, which constitutes a novel way to co-regulate neighboring genes. In agreement with recent findings, low abundance antisense RNAs covering 44% of the annotated genes were captured by co-immunoprecipitation with RNase III mutant proteins. Thus, in addition to gene regulation, RNase III is associated with RNA quality control of pervasive transcription. Overall, this study illustrates the complexity of post-transcriptional regulation mediated by RNase III

Coordinated Destruction of Cellular Messages in Translation Complexes by the Gammaherpesvirus Host Shutoff Factor and the Mammalian Exonuclease Xrn1

Several viruses encode factors that promote host mRNA degradation to silence gene expression. It is unclear, however, whether cellular mRNA turnover pathways are engaged to assist in this process. In Kaposi's sarcoma-associated herpesvirus this phenotype is enacted by the host shutoff factor SOX. Here we show that SOX-induced mRNA turnover is a two-step process, in which mRNAs are first cleaved internally by SOX itself then degraded by the cellular exonuclease Xrn1. SOX therefore bypasses the regulatory steps of deadenylation and decapping normally required for Xrn1 activation. SOX is likely recruited to translating mRNAs, as it cosediments with translation initiation complexes and depletes polysomes. Cleaved mRNA intermediates accumulate in the 40S fraction, indicating that recognition occurs at an early stage of translation. This is the first example of a viral protein commandeering cellular mRNA turnover pathways to destroy host mRNAs, and suggests that Xrn1 is poised to deplete messages undergoing translation in mammalian cells