Construction of Novel Phytochelatins by Overlap Oligonucleotides

Synthetic phytochelatins are protein analogs of phytochelatin with similar heavy metal binding affinities that can be easily produced from a synthetic DNA template. We design synthetic phytochelatin [(Glu-Cys)n Gly] linked to hexahistidine by viral linker peptide and then followed by gene synthesis and cloning of it. Then peptide coding gene (synthetic phytochelatin with linker and hexahistidine) was designed exactly and constructed with step by step methods by overlapping oligonucleotides using T4 DNA Ligase. Finally, synthesized gene amplified by PCR, cloned in pTZ57R/T and transformed to Escherichia coli (DH5α). The results of sequencing show that some types of synthetic phytochelatin (EC4, EC12, and EC20) with linker and hexahistidine were constructed and cloned in vector

Unexpected relaxation dynamics of a self-avoiding polymer in cylindrical confinement

We report extensive simulations of the relaxation dynamics of a self-avoiding polymer confined inside a cylindrical pore. In particular, we concentrate on examining how confinement influences the scaling behavior of the global relaxation time of the chain, t, with the chain length N and pore diameter D. An earlier scaling analysis based on the de Gennes blob picture led to t ~ N^2D^(1/3). Our numerical effort that combines molecular dynamics and Monte Carlo simulations, however, consistently produces different t-results for N up to 2000. We argue that the previous scaling prediction is only asymptotically valid in the limit N >> D^(5/3) >> 1, which is currently inaccessible to computer simulations and, more interestingly, is also difficult to reach in experiments. Our results are thus relevant for the interpretation of recent experiments with DNA in nano- and micro-channels.Comment: 10 pages, 11 figure

Complications of transcatheter closure in patent ductus arteriosus patients

Background: Published studies have shown excellent success rates and also low rates of life-threatening complications with trans-catheter closure of patent ductus arteriosus (PDA). However, most studies to date have been conducted in developed nations and reports from developing countries such as Iran are lacking. Objectives: To report our experience with trans-catheter closure of patent ductus arteriosus (PDA) and compare two devices and complications. Patients and Methods: From 2007 to 2011, 72 consecutive PDA cases were prospectively enrolled. For PDAs � 2 mm, the coiling method was employed whereas patients with PDA > 2 mm received Amplatzer duct occlusion (ADO). Success was defined as trivial residual shunt (< 1 mm) in the echocardiography performed 30 minutes after the procedure. After the procedure, the patients were admitted to the pediatric cardiology ward and observed for 72 hours during which the occurrence of major and minor complications was monitored. Major complications included cardiac arrest, displacement and/or embolization of the device and patient needing blood transfusion due to severe hemorrhage. Sampling was based on convenience method and the patients who had significant pulmonary artery hypertension were excluded from our study. Statistical analyses were done using SPSS software version 23 (IBM corp., New York, United States). Continuous variables are presented as mea

Teacher and student views on the feasibility of peer to peer education as a model to educate 16–18 year olds on prudent antibiotic use—a qualitative study

Peer education (PE) has been used successfully to improve young peoples’ health-related behaviour. This paper describes a qualitative evaluation of the feasibility of university healthcare students delivering PE, covering self-care and antibiotic use for infections, to biology students in three UK schools (16–18 years), who then educated their peers. Twenty peer educators (PEds) participated in focus groups and two teachers took part in interviews to discuss PE feasibility. Data were analysed inductively. All participants reported that teaching students about antibiotic resistance was important. PE was used by PEds to gain communication skills and experience for their CV. PEds confidence increased with practice and group delivery. Interactive activities and real-life illness scenarios facilitated enjoyment. Barriers to PE were competing school priorities, no antibiotic content in the non-biology curriculum, controlling disruptive behaviour, and evaluation consent and questionnaire completion. Participation increased PEds’ awareness of appropriate antibiotic use. This qualitative study supports the feasibility of delivering PE in schools. Maximising interactive and illness scenario content, greater training and support for PEds, and inclusion of infection self-care and antibiotics in the national curriculum for all 16–18-year olds could help facilitate greater antibiotic education in schools. Simplifying consent and data collection procedures would facilitate future evaluations

Threshold Based Skin Color Classification

In this paper, we presented a new formula for skin classification. The proposed formula can overcome sensitivity to noise. Our approach was based multi-skin color Hue, Saturation, and Value color space and multi-level segmentation. Skin regions were extracted using three skin color classes, namely the Caucasoid, Mongolid and Nigroud. Moreover, in this formula, we adopted Gaussian-based weight k-NN algorithm for skin classification. The experiment result shows that the best result was achieved for Caucasoid class with 84.29 percent fmeasure

Peer-education as a tool to educate on antibiotics, resistance and use in 16–18-year-olds: A feasibility study

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Peer education (PE) interventions may help improve knowledge and appropriate use of antibiotics in young adults. In this feasibility study, health-care students were trained to educate 16–18 years old biology students, who then educated their non-biology peers, using e-Bug antibiotic lessons. Knowledge was assessed by questionnaires, and antibiotic use by questionnaire, SMS messaging and GP record searches. Five of 17 schools approached participated (3 PE and 2 control (usual lessons)). 59% (10/17) of university students and 28% (15/54) of biology students volunteered as peer-educators. PE was well-received; 30% (38/127) intervention students and 55% (66/120) control students completed all questionnaires. Antibiotic use from GP medical records (54/136, 40% of students’ data available), student SMS (69/136, 51% replied) and questionnaire (109/136, 80% completed) data showed good agreement between GP and SMS (kappa = 0.72), but poor agreement between GP and questionnaires (kappa = 0.06). Median knowledge scores were higher post-intervention, with greater improvement for non-biology students. Delivering and evaluating e-Bug PE is feasible with supportive school staff. Single tiered PE by university students may be easier to regulate and manage due to time constraints on school students. SMS collection of antibiotic data is easier and has similar accuracy to GP data

Antibacterial properties of bacterial nanocellulose functionalized with metal nanoparticles via in situ synthesis

[Excerpt] Wound infections are generally caused by pathogens and multidrug-resistant (MDR) strains that render the administration of antibiotics ineffective. An alternative is to treat infected wounds at the initial stage using a fibrous bionanopolymer, bacterial nanocellulose (BNC), functionalized with antimicrobial metal nanoparticles (MNPs). BNC is a highly promising wound dressing due to its very high-water retention capacity (> 99 %) and high porosity. Such properties enable the absorbance of exudates, whilst maintaining the environment moist allowing the exchange of air. However, BNC is absent of antibacterial properties, thus gold (Au), copper (Cu), and copper oxide (Cu2O) NPs were incorporated within the nanofibrous structure of the biopolymer via in situ synthesis

Agreement of the clinician’s choice of archwire selection on conventional and virtual models

Objectives: To compare archwire selection on dental casts with archwire selection using a three-dimensional (3D) software program (OrthoAid) and assess agreement between clinicians. Materials and Methods: The best-fitting archwires were selected for dental casts of 100 patients with malocclusion using two approaches by three orthodontists. The first method was to visually determine the fitness of five preformed nickel titanium archwires to the arch form on a dental cast (subjective method). The second method was archwire selection on a virtual image of the same cast by means of 3D software (objective method). Agreement between selections performed by the orthodontists was calculated using Kappa statistics. The accuracy of fit of the archwires to the curves fitted to the arch form was also calculated or reversely assessed by means of the root mean square (RMS) for both methods using the Dahlberg formula. Results: The mean RMS of the distances between the patient arch forms and the archwires for the subjective method was 1.163–1.366 mm. The agreement of selections between orthodontists was 42%–58% (Kappa ranged from .074 to .382). Using the 3D software (objective method), the mean RMS decreased to 0.966–1.171 mm, and agreement increased to 47% to 84% (Kappa ranged from .444 to .747). Conclusions: The use of 3D computer software for archwire selection in patients with malocclusion provided better adaptation and interexaminer reliability

Magnetic hardening of Fe30Co70nanowires

3d transition metal-based magnetic nanowires (NWs) are currently considered as potential candidates for alternative rare-earth-free alloys as novel permanent magnets. Here, we report on the magnetic hardening of FeConanowires in anodic aluminium oxide templates with diameters of 20 nm and 40 nm (length 6 μm and 7.5 μm, respectively) by means of magnetic pinning at the tips of the NWs. We observe that a 3-4 nm naturally formed ferrimagnetic FeCo oxide layer covering the tip of the FeCo NW increases the coercive field by 20%, indicating that domain wall nucleation starts at the tip of the magnetic NW. Ferromagnetic resonance (FMR) measurements were used to quantify the magnetic uniaxial anisotropy energy of the samples. Micromagnetic simulations support our experimental findings, showing that the increase of the coercive field can be achieved by controlling domain wall nucleation using magnetic materials with antiferromagnetic exchange coupling, i.e. antiferromagnets or ferrimagnets, as a capping layer at the nanowire tips.We acknowledge funding from the European Community's Seventh Framework Programme (FP7-NMP) under grant agreement no. 280670 (REFREEPERMAG)