Serum levels of lead and copper in a group of Egyptian children with bronchial asthma

Background: Copper and lead are trace elements required for the activity of antioxidant enzymes and changes in their levels may lead to reduction in antioxidant activities in asthma.Objective: Our study aims to investigate the serum levels of copper and lead in asthmatic children in correlation to disease severity to anticipate their role as oxidant defenders in this disease.Methods: We enrolled 45 children who were divided into two groups: group 1 included 30 asthmatic children during disease quiescence and group 2 included 15 clinically healthy children matched for age and sex as a control group. Patients were subjected to: history taking, clinical examination, spirometry before and after bronchodilator therapy, complete blood counting, and measurement of serum levels of total IgE, copper and lead (in patients and controls).Results: Patients’ group had significantly higher serum levels of lead (mean 8.2±3.1 μg/dl) and copper (122±31.5 μg/dl) in comparison to controls (mean 5.7±2.3 μg/dl and103.3±21.1 μg/dl respectively). Serum lead and copper levels were higher among patients with moderate persistent asthma than those with mild asthma. Serum total IgE levels correlated positively with serum lead levels among the asthmatic children. However, serum lead and copper levels did not correlate with any of the measured pulmonary function parameters tested.Conclusion: Increased serum level of lead and copper were high in a group of children with bronchial asthma in children and this was more evident in moderate than mild cases.Keywords: children; trace elements; lead; copper; spirometry; bronchial asthma; antioxidants; severit

RESPONSE OF STRAWBERRY PLANTS TO SHORTENING DAY LENGTH , SHADING AND COLD STORAGE UNDER EGYPTIAN CONDITIONS

This experiment was performed in 2004/2005 and 2005/2006 seasons at El-Bosaly Protected Cultivation Experimental Site, of the Central Laboratory for Agricultural Climate (CLAC), 15 Km west of Rosetta. The current study was conducted in open field to investigate the growth and productivity of strawberry plants under the shading and cold storage methods. Two strawberry cultivars were used, i.e., Camarosa (cv.) and Yael (cv.). Transplants were set up on 15 of March to 15 of September in both two seasons of 2004-2005 and 2005-2006, respectively under El-Bosaly conditions. Seven treatments were used i.e., short day, 40% shading, 73% shading, three periods cold storage at 5Co, i.e., (24h, 48h and 73h) and control. Results indicated that the application of 73% shading treatment was the most effective in reducing maximum and minimum of air temperature and radiation, followed by 40% shading, whereas, the highest value of maximum and minimum air temperature, radiation were observed in control treatment throughout the two growing seasons. In addition, 73% shading treatment produced the tallest plants and the highest number of leaves per plant and leaf area per plant. Moreover, flowering and chlorophyll in leaves were increased by application of short day treatment. Also, fruit characters such as average weight and number of fruits per plant were the highest values with 40% shading. Chemical components of fruits, i.e., T.S.S were increased with 73% shading treatment. When plants were grown under 40% shading treatment, followed by 73% shading, while cold storage for 72 h treatment gave the lowest values. Neither cultivars nor the interaction between cultivars and tested factors had any significant effects on many studied characters

Global overview of the management of acute cholecystitis during the COVID-19 pandemic (CHOLECOVID study)

Background: This study provides a global overview of the management of patients with acute cholecystitis during the initial phase of the COVID-19 pandemic. Methods: CHOLECOVID is an international, multicentre, observational comparative study of patients admitted to hospital with acute cholecystitis during the COVID-19 pandemic. Data on management were collected for a 2-month study interval coincident with the WHO declaration of the SARS-CoV-2 pandemic and compared with an equivalent pre-pandemic time interval. Mediation analysis examined the influence of SARS-COV-2 infection on 30-day mortality. Results: This study collected data on 9783 patients with acute cholecystitis admitted to 247 hospitals across the world. The pandemic was associated with reduced availability of surgical workforce and operating facilities globally, a significant shift to worse severity of disease, and increased use of conservative management. There was a reduction (both absolute and proportionate) in the number of patients undergoing cholecystectomy from 3095 patients (56.2 per cent) pre-pandemic to 1998 patients (46.2 per cent) during the pandemic but there was no difference in 30-day all-cause mortality after cholecystectomy comparing the pre-pandemic interval with the pandemic (13 patients (0.4 per cent) pre-pandemic to 13 patients (0.6 per cent) pandemic; P = 0.355). In mediation analysis, an admission with acute cholecystitis during the pandemic was associated with a non-significant increased risk of death (OR 1.29, 95 per cent c.i. 0.93 to 1.79, P = 0.121). Conclusion: CHOLECOVID provides a unique overview of the treatment of patients with cholecystitis across the globe during the first months of the SARS-CoV-2 pandemic. The study highlights the need for system resilience in retention of elective surgical activity. Cholecystectomy was associated with a low risk of mortality and deferral of treatment results in an increase in avoidable morbidity that represents the non-COVID cost of this pandemic

Evaluation of surgical approaches to infratemporal and pterygopalatine fossae

Abstract Background Tumors in the pterygopalatine fossa (PPF) and infratemporal fossa (ITF) are still challenging to surgeons because of their deep location and proximity to various arteries and cranial nerves. This study aimed to evaluate the access to infratemporal fossa and pterygopalatine fossa through anterior transmaxillary, modified endoscopic transnasal transmaxillary approaches. The study was a prospective comparative study conducted on 20 patients having a mass in pterygopalatine or infratemporal fossa. History, examination, computed tomography, and magnetic resonance imaging were performed for all patients. Nine patients were operated with anterior transmaxillary approach and eleven patients were operated with endoscopic transnasal transmaxillary approach. The operative duration, intraoperative blood loss, and operative difficulty were assessed. Patients were assessed 1 week postoperatively using a nasal surgical questionnaire for postoperative nasal symptoms. Endoscopic assessment of the nose was done 1 week postoperative to assess the degree of crusting and 4 weeks postoperative to assess nasal adhesions. Postoperative complications were assessed for both groups. Results There was a significant difference between the two groups regarding operative duration and blood loss favoring the endoscopic group. There was a non-significant difference between the two groups regarding operative difficulty. There was a significant difference between the two groups regarding nasal obstruction, crustation, bleeding, sneezing, secretion, and nasal pain. There was a significant difference between the two groups regarding the endoscopic assessment for nasal crusting and adhesions. Facial swelling and numbness of the face were significantly more in the open group compared with the endoscopic group. Conclusion Endoscopic endonasal transmaxillary approach had less trauma and lower complication rate while anterior transmaxillary was technically feasible and offered excellent surgical access with easy lateral expansion toward the infratemporal fossa. Both approaches had comparable operative difficulty and acceptable postoperative quality of life

Value of Foxp3 expressing T-regulatory cells in renal tissue in lupus nephritis; an immunohistochemical study

Background: Forkhead box P3 (Foxp3) functions as a master regulator in the development and function of T-regulatory (Treg) cells. Recent studies have shown that autoimmune diseases including systemic lupus erythematosus (SLE) are associated with an imbalance with the Treg cells and T helper (Th) subtypes. Objectives: To evaluate immunohistochemical expression of Foxp3 positive Treg cells in lupus nephritis (LN) and analyze its association with clinicopathologic parameters. Materials and Methods: Renal biopsy specimens of 50 patients with LN were studied. Specimens were divided into; group A; 25 LN cases without proliferative activity (Class II and V) and group B: 25 cases with proliferative activity (Class III and IV). Immunohistochemical staining for anti-human Foxp3 antibody and grading from grade 0 to grade 3 was done. Results: Foxp3 expression in group A was (grade 0 in 14 [56.0%], grade +1 in 11 [44.0 %]) in comparison to group B (grade +1 in 6 [24.0%], grade +2 in 11 [44.0%] and grade +3 in 8 [32.0%]) (P < 0.001). Foxp3 expression was significantly correlated to National Institutes of Health (NIH) activity and chronicity indices (P < 0.05), as well as serum creatinine (P < 0.01) in both groups A and B and there was a highly significant correlation with proteinuria (P < 0.01) in group B with proliferative LN. Conclusions: Immunohistochemical Foxp3 expression in renal tissue was higher in proliferative versus non-proliferative LN and is associated with activity and severity of LN. Further studies are needed to determine its prognostic value in LN

Influence of early feeding practices on biomarkers of cardiovascular disease risk in later life

Background: An analysis of risk factors linked to ischemic heart disease (IHD) shows a strong link between these risk factors and early feeding practices. Aim of the work: The aim of this study was to evaluate cardiac biomarkers that could predict cardiovascular disease (lipid profile and highly sensitive C-reactive protein (hs-CRP) for both mothers and their children and demonstrate their associations with early feeding practices. Methods: This was a cross-sectional study comprising one hundred twenty pairs of mother and their children, one half of whom were exclusively breastfed for 6 months, the other half their children were formula fed from birth. The groups were matched for age and sex. Full feeding history was taken for children and assessments of risk behaviour of cardiovascular disease including Anthropometric measurements to assess Body Mass Index, blood pressures and blood samples for lipid profile and hs-CRP for both mothers and children. Results: There was a statistically significant difference between the two groups regarding hs-CRP as it was higher in mothers and their children who were artificially feed than mothers and their children who were breastfeed (m = 3.3 + 2.2–1.72 ± 1.96, 2.08 ± 1.64–0.84 ± 1.09 respectively), however there was no a statistically significant difference for both mothers and their children regarding lipid profile. Conclusion: Early feeding practices can influence the development of cardiovascular diseases as breast fed infants and their mothers had lower hs- CRP levels which is considered as a biomarker of CVD risk