Planificación estratégica y mejora de la calidad en las universidades

En el lomo: 4

Eskola 2.0: a complete course. Time for evaluation

En el año 2009, se puso en marcha el proyecto de integración de las tecnologías Eskola 2.0. en el País Vasco. Dicho programa, incluido en el programa Escuela 2.0 promovido por el Ministerio de Educación de España, se ha implantado en el tercer ciclo de educación primaria y primero ciclo de Educación Secundaria, digitalizando las aulas con ordenadores portátiles para cada alumno/a y profesor/a, Pizarras Digitales Interactivas y acceso wifi. Desde septiembre del curso lectivo 2009/2010, hemos desarrollado un proceso de investigación-acción participativa, teniendo la posibilidad de vivir desde dentro lo que supone para el profesorado como alumnado llevar adelante dicho programa y la utilización de estos nuevos recursos digitales. En este artículo describimos el proceso de investigación seguido y abordamos el análisis de la implantación del proyecto Eskola 2.0. en un centro escolar de educación primaria de la provincia de Gipuzkoa, desde diferentes perspectivas. Por un lado, los puntos de vista del profesorado, la dirección del centro y una investigadora externa que acompaña tanto la formación como la implementación del proyecto en el aula. Por otro lado, el punto de vista del alumnado, la percepción que tiene de la experiencia vivida, el sentido que le dan a una herramienta y recurso que sobre todo relacionan con el juego y la diversión.Since 2009, in the Basque Country we are immersed in the project Eskola2.0. The project, is included in another project called Escuela 2.0 promoted by the Ministry of Education of Spain. This project, was implanted in the third cycle of primary education and now is been implemented in the first cycle of secondary education. The program consists in digitalizing classrooms with laptops for each student, teacher and whiteboards and wifi access. During the school course 2009/2010, we had been able to know how the teachers and students live the project. When there are material resources like laptops, these, are to be used, but is in the process when we can see the lack of pedagogical and methodological orientation. In this case, the perception of the different people involved in the program is not the same. This article arises from the point of view of teachers, the school direction and an external researcher accompanying the formation and implementation of the project in the classrooms. From the students' point of view, the perception of the program, at first, brings the meaning they give to a tool and resource like a laptop: basically for play and fun

Integración de tecnología digital en un centro de educación infantil y primaria de la comunidad autónoma vasca: de las políticas a las prácticas. Una investigación narrativa.

363 p.Este trabajo de investigación narrativa se ocupa de analizar en qué manera la puesta en marcha del programa Escuela 2.0 en el centro de Educación Infantil y Primaria San Martin Agirre de Bergara contribuyó al desarrollo de estrategias innovadoras en la práctica docente. Entre el estudio de caso y la investigación-acción, esta investigación autobiográfica bucea también en las razones de índole organizativa o administrativa que obstaculizaron este desarrollo, estudia el tipo de resistencias observadas en el profesorado a la hora de adaptar su quehacer diario a los desafíos que la sociedad de la información y el conocimiento plantea a cualquier profesional de la enseñanza, y ahonda en lo relacional de la experiencia educativa de un equipo directivo que estrenó su cargo el mismo curso escolar en el que se puso en marcha dicho programa de introducción masiva de tecnología digital en las aulas, una dirección que deseaba trascender la vertiente gestora para detenerse en el sentido de la multiplicidad de factores que rubrican el desempeño de la responsabilidad directiva desde una mirada centrada en la relación pedagógica

Insights Into SND1 Oncogene Promoter Regulation

The staphylococcal nuclease and Tudor domain containing 1 gene (SND1), also known as Tudor-SN, TSN or p100, encodes an evolutionarily conserved protein with invariant domain composition. SND1 contains four repeated staphylococcal nuclease domains and a single Tudor domain, which confer it endonuclease activity and extraordinary capacity for interacting with nucleic acids, individual proteins and protein complexes. Originally described as a transcriptional coactivator, SND1 plays fundamental roles in the regulation of gene expression, including RNA splicing, interference, stability, and editing, as well as in the regulation of protein and lipid homeostasis. Recently, SND1 has gained attention as a potential disease biomarker due to its positive correlation with cancer progression and metastatic spread. Such functional diversity of SND1 marks this gene as interesting for further analysis in relation with the multiple levels of regulation of SND1 protein production. In this review, we summarize the SND1 genomic region and promoter architecture, the set of transcription factors that can bind the proximal promoter, and the evidence supporting transactivation of SND1 promoter by a number of signal transduction pathways operating in different cell types and conditions. Unraveling the mechanisms responsible for SND1 promoter regulation is of utmost interest to decipher the SND1 contribution in the realm of both normal and abnormal physiology.Work in the authors' lab is supported by Spanish Ministry of Economy, Industry and Competitiveness grants SAF-2015-64352-R and RTC-2015-3693-1 and Basque Government grants IT-971-16 and KK2018-00090

Abandonment of traditional livestock grazing reduces soil fertility and enzyme activity, alters soil microbial communities, and decouples microbial networks, with consequences for forage quality in Mediterranean grasslands

Extensive livestock grazing is a global human activity. In the Iberian Peninsula, extensive grazing and seminatural grasslands and open woodlands such as dehesas have co-evolved with human use for millennia. However, social, demographic, and economic factors are now pushing this traditional activity towards both conventional intensification and land abandonment, with consequences for the biodiversity and functioning of these seminatural ecosystems. Soils can be particularly affected by grazing abandonment due to the cessation of inputs of pre-processed organic matter (dungs and urine) and of trampling, with still poorly understood consequences for the composition, network configuration, and activity of soil microbial communities and the capacity of soils to store C. In this work, we used 20 pairs of adjacent plots (40 plots in total) located in seminatural grasslands from central Spain. For each pair, one plot was extensively grazed by livestock and the other one was abandoned. We evaluated the effects of extensive grazing abandonment on soil fertility (C and N contents, and P and K bioavailability), forage quality (fibre and protein content), and soil microbial community composition (amplicon sequencing of 16 S [bacteria] and ITS [fungi]), network coupling, and activity (extracellular hydrolytic enzymes linked to the biogeochemical cycling of C, N, P, and S). Grazing resulted in higher soil fertility in terms of C, N, and P, and grassland forage quality (lower fibre). Grazing also affected soil microbial community composition, but not richness or diversity. These effects occurred primarily through changes in nutrients and soil water availability. Actinobacteria significantly increased in abandoned plots, while Acidobacteria, Verrucomicrobia, and Planctomycetes decreased. Bacterial and, particularly, fungal networks were generally less coupled in abandoned plots. Furthermore, grazing resulted in greater soil enzyme activity via direct effects. These results support the notion that extensive grazing with intermediate stocking rates provides a positive effect on grass quality, soil fertility, nutrient cycling, and microbial network configuration, and thus warn about the potential negative effects of land abandonment

Expression of Adenosine A2B Receptor and Adenosine Deaminase in Rabbit Gastric Mucosa ECL Cells

Adenosine is readily available to the glandular epithelium of the stomach. Formed continuously in intracellular and extracellular locations, it is notably produced from ATP released in enteric cotransmission. Adenosine analogs modulate chloride secretion in gastric glands and activate acid secretion in isolated parietal cells through A2B adenosine receptor (A2BR) binding. A functional link between surface A2BR and adenosine deaminase (ADA) was found in parietal cells, but whether this connection is a general feature of gastric mucosa cells is unknown. Here we examine whether A2BR is expressed at the membrane of histamine-producing enterochromaffin-like (ECL) cells, the major endocrine cell type in the oxyntic mucosa, and if so, whether it has a vicinity relationship with ADA. We used a highly homogeneous population of rabbit ECL cells (size 7.5-10 mu m) after purification by elutriation centrifugation. The surface expression of A2BR and ADA proteins was assessed by flow cytometry and confocal microscopy. Our findings demonstrate that A2BR and ADA are partially coexpressed at the gastric ECL cell surface and that A2BR is functional, with regard to binding of adenosine analogs and adenylate cyclase activation. The physiological relevance of A2BR and ADA association in regulating histamine release is yet to be explained.We thank all the members of R. Franco and C. Lluis research group (University of Barcelona, Spain) for assistance with immunostaining experiments and the advice received. We thank E. Castro and M.T. Miras-Portugal (Complutense University of Madrid, Spain) for assistance with microfluorimetry experiments. This work was supported by grants from the Basque Government (IT-971-16) and the University of the Basque Country (UFI11/20)

Teacher Education, Educational Technology and Teacher Digital Identity

La perspectiva de la identidad docente digital es una oportunidad para repensar la influencia de la postmodernidad sobre la Escuela, el curriculum y los docentes. Basándonos en Bauman, Wenger o Gergen hemos definido la identidad digital docente, como un proceso dinámico y permanente que implica dotar de sentido y reinterpretar las propias creencias, valores y experiencias docentes a la luz de los nuevos contextos y marcos de relaciones en la sociedad contemporánea caracterizada por la digitalización de la experiencia humana. En este artículos hemos relacionado la identidad digital docente con los cambios acaecidos en la sociedad contemporánea, las concepciones del conocimiento, la escuela o la autoridad curricular. Reivindicamos un enfoque de la tecnología educativa crítica basada en la emancipación y convivencialidad, que incorpore la reflexión sobre cómo nos configuramos a partir de las experiencias digitales y de la cultura visual. Terminamos este artículo reinvindicando la pedagogía narrativa como estrategia de biografización de la experiencia digital docente.The prospect of digital teaching identity is an opportunity to rethink the influence of postmodernism on the school, the curriculum and teachers. Based on Bauman, Wenger or Gergen we have defined the teaching digital identity, as a dynamic and ongoing process that involves making sense and reinterpret the beliefs, values and educational experiences in light of new contexts and frames of relationships in contemporary society characterized by scanning the human experience. In this articles we teaching digital identity associated with the changes in contemporary society, conceptions of knowledge, the school curriculum authority. We demand an approach of educational technology criticism based on emancipation and conviviality, to incorporate the reflection on how we configure from the digital experiences and visual culture. This article claiming ended narrative strategy biographization pedagogy as digital teaching experience.peerReviewe

Humoral immune response in hens naturally infected with Salmonella Enteritidis against outer membrane proteins and other surface structural antigens

A simple procedure for obtaining surface exposed antigens of Salmonella Enteritidis is described. A heat treatment of whole bacteria in saline solution induced the release of small membrane vesicles containing outer membrane components as well as surface appendage components, such as fimbriae and flagellin. The characterization of the structural components of this extract, called HE, was established by SDS-PAGE and immunoblotting using polyclonal and monoclonal specific antibodies. Five major groups of proteins were identified: flagellin, porins, OmpA, SEF21 and SEF14 fimbriae. The immunogenicity of these proteins was studied by immunoblotting with serum samples from naturally infected hens. Flagellin, porins, OmpA, SEF14 and SEF21 fimbriae were immunogenic in the S. Enteritidis infected hens (frequency of reactants: 47.3, 97.3, 64.7, 50.0 and 60.8%, respectively); porins also reacted with sera from non infected hens (66.7%). The immunogenicity of these antigens in infected birds provide promise that they may serve as components of an effective subcellular vaccine for poultry salmonellosis

Upregulated phospholipase D2 expression and activity is related to the metastatic properties of melanoma

[EN] The incidence rates of melanoma have increased steadily in recent decades and nearly 25% of the patients diagnosed with early-stage melanoma will eventually develop metastasis, for which there is currently no fully effective treatment. The link between phospholipases and tumors has been studied extensively, particularly in breast and colon cancers. With the aim of finding new biomarkers and therapeutic options for melanoma, the expression of different phospholipases was assessed in 17 distinct cell lines in the present study, demonstrating that phospholipase D2 (PLD2) is upregulated in metastatic melanoma as compared to normal skin melanocytes. These results were corroborated by immunofluorescence and lipase activity assays. Upregulation of PLD2 expression and increased lipase activity were observed in metastatic melanoma relative to normal skin melanocytes. So far, the implication of PLD2 activity in melanoma malignancies has remained elusive. To the best of our knowledge, the present study was the first to demonstrate that the overexpression of PLD2 enhances lipase activity, and its effect to increase the proliferation, migration and invasion capacity of melanoma cells was assessed with XTT and Transwell assays. In addition, silencing of PLD2 in melanoma cells reduced the metastatic potential of these cells. The present study provided evidence that PLD2 is involved in melanoma malignancy and in particular, in its metastatic potential, and established a basis for future studies evaluating PLD2 blockade as a therapeutic strategy to manage this condition.This study was supported by grants from the University of the Basque Country/EHU (grant no. GIU17/066) and Ministerio de Economia y Competividad MINECO-ONCOFINDER of the Spanish Government (grant no. RTC.2015-3693-1)