14 research outputs found
Biochemical and haematological profile of pheasant hens during the laying period
The present paper provides new experimental data on the biochemical and haematological profile of blood in pheasant hens, and points out the changes in both biochemical and haematological parameters that occur during the laying period. Significant effects of egg laying on both the biochemical and the haematological blood parameters of pheasant hens were found. Biochemical analyses revealed a significant increase in the metabolites cholesterol, uric acid, lactate, the enzyme aspartate aminotransferase (AST) and the minerals calcium and phosphorous, as well as a significant decrease in total protein, albumin and glucose in the course of the laying period. Haematological analyses revealed a significant increase in the count of leukocytes, lymphocytes, eosinophils, basophils and monocytes due to egg laying. In addition, the erythrocyte count and haemoglobin content significantly decreased in the middle of the laying period and then rebounded at the end of the laying period. The haematocrit content gradually decreased till the end of the laying period. All together, the results of this study underline the impact of the reproduction status of pheasant hens on basic blood parameters. The biochemical and haematological values presented in this study may be of help in assessing disease conditions in laying pheasant hens
Recovery ability of common carp (Cyprinus carpio) after a short-term exposure to terbuthylazine
Effects of a high terbuthylazine concentration (3.3 mg/l) on Cyprinus carpio were studied using
a commercial herbicide formulation Click 500 SC (terbuthylazine 500 g/l). The fish were exposed to
the pesticide for 24 h and allowed to recover for 6 days. Biometric parameters, plasma biochemical
parameters and biomarkers of oxidative stress as well as histopathological changes in selected tissues
were assessed on day 1 and 7. After a 24-h exposure, there were significant alterations found in the
activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as well as in the
plasma concentrations of glucose, natrium, chlorides, calcium and phosphorus. Hepatosomatic index,
plasma albumin and lactate reflected the treatment with a delay. Ion levels and ALT were found to be
restored after a 6-day recovery period, which was too short for AST activity and glucose to diminish to
the control levels. The histopathological examination revealed disorders in the gills of the exposed
fish, however, the changes were not detected after a 6-day recovery period. The study shows high
regeneration potential of the fish
Effects of subchronic exposure to atrazine on zebrafish (Danio rerio)
The aim of this study was to investigate the effects of subchronic exposure to atrazine on fish growth and the development of histopathological changes in selected organs (gill, kidney, liver) in Danio rerio. Juvenile growth tests were performed on D. rerio according to OECD method No. 215. For 28 days, fish at an initial age of 30 days were exposed to the environmental atrazine concentration commonly detected in Czech rivers (0.3 μg/L) and a range of sublethal concentrations of atrazine (3.0, 30.0 and 90.0 μg/L). The results showed decreasing growth rates and morphological changes in the liver (dystrophic lesions of hepatocytes) at 90.0 μg/L of atrazine. The environmental concentration of atrazine in Czech rivers did not have any effect on fish growth and development of histopathological changes in D. rerio. The value of NOEC was 30.0 μg/L and the value of LOEC was 90.0 μg/L