Connexin 43 is overexpressed in human fetal membrane defects after fetoscopic surgery

This project was funded by the RoseTrees Trust (M400, TTC), the QMUL Life Sciences Initiative, Institutional Strategic Support Fund from the Wellcome Trust (105626/Z/14/Z, TTC) and supported by researchers at the National Institute for Health Research, University College London Hospitals Biomedical Research Centre (ALD)

Achievement goals, behavioural engagement, and mathematics achievement: A mediational analysis

Previous studies have shown that mastery-approach and performance-approach goals can be positively associated with adaptive educational outcomes. Few studies, however, have examined links with behavioural engagement. The aim of the present study was to examine whether behavioural engagement mediated relations between mastery-approach and performance-approach goals and subsequent achievement in mathematics. Data were collected from 1057 students aged 9 to 11 years in a longitudinal design over the course of a single school year. Results showed that a mastery-approach, but not a performance-approach, goal predicted behavioural engagement. Behavioural engagement, in turn, predicted mathematics achievement. Furthermore, behavioural engagement mediated relations between mastery-approach and subsequent mathematics achievement. This study contributes to the evidence base for the adaptive role of mastery-approach which can be encouraged by students setting personal best goals, teachers ensuring that feedback is task-focused, and that the classroom climate is mastery-focused

Reciprocal relations between students' academic enjoyment, boredom, and achievement over time

The control-value theory (CVT) proposes that achievement emotions and academic achievement show reciprocal effects over time. Previous studies have examined how achievement emotions predict subsequent achievement. However, evidence is limited for whether achievement can also predict achievement emotions. To examine these reciprocal relations, data were collected about two achievement emotions: enjoyment and boredom, and mathematics achievement over four waves in a single school year in primary school students in Years 5 and 6. Results from structural equation modeling supported reciprocal relations between emotions and achievement. Higher enjoyment and lower boredom predicted greater subsequent achievement and, in turn, greater academic achievement predicted subsequent greater enjoyment and lower boredom. Furthermore, the relations between emotions over time were mediated by achievement. These findings build on the evidence base for CVT and further understanding of relations between achievement emotions and academic achievement in younger students. © 2017 Elsevier Ltd

Potential sealing and repair of human FM defects after trauma with peptide amphiphiles and Cx43 antisense

OBJECTIVE: We examined whether peptide amphiphiles functionalised with adhesive, migratory or regenerative sequences could be combined with amniotic fluid (AF) to form plugs that repair fetal membrane (FM) defects after trauma and co-culture with connexin 43 (Cx43) antisense. METHODS: We assessed interactions between peptide amphiphiles and AF and examined the plugs in FM defects after trauma and co-culture with the Cx43antisense. RESULTS: Confocal microscopy confirmed directed self-assembly of peptide amphiphiles with AF to form a plug within minutes, with good mechanical properties. SEM of the plug revealed a multi-layered, nanofibrous network that sealed the FM defect after trauma. Co-culture of the FM defect with Cx43 antisense and plug increased collagen levels but reduced GAG. Culture of the FM defect with peptide amphiphiles incorporating regenerative sequences for 5 days, increased F-actin and nuclear cell contraction, migration and polarization of collagen fibers across the FM defect when compared to control specimens with minimal repair. CONCLUSIONS: Whilst the nanoarchitecture revealed promising conditions to seal iatrogenic FM defects, the peptide amphiphiles need to be designed to maximize repair mechanisms and promote structural compliance with high mechanical tolerance that maintains tissue remodeling with Cx43 antisense for future treatment

Novel application and validation of in vivo micro‐CT to study bone modelling in 3D

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/149362/1/ocr12265.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/149362/2/ocr12265_am.pd

Expectancy of Success, Attainment Value, Engagement, and Achievement: A Moderated Mediation Analysis

The aim of this study was to examine how expectancy of success, attainment value, and their interaction predict behavioural engagement, and how behavioural engagement, in turn, predict achievement. Data were collected from 586 English students aged 10-11 years in their final year of primary school. Expectancy of success was positively related to subsequent achievement directly and indirectly, mediated by behavioural engagement over and above the variance accounted for by prior achievement and behavioural engagement. Indirect relations from expectancy of success to achievement were moderated by attainment value. Higher attainment value protected performance from low expectancy of success by increasing behavioural engagement. The compensatory role of high attainment value diminished at higher levels of expectancy of success

Expectancy of Success, Attainment Value, Engagement, and Achievement: A Moderated Mediation Analysis

The aim of this study was to examine how expectancy of success, attainment value, and their interaction predict behavioural engagement, and how behavioural engagement, in turn, predict achievement. Data were collected from 586 English students aged 10-11 years in their final year of primary school. Expectancy of success was positively related to subsequent achievement directly and indirectly, mediated by behavioural engagement over and above the variance accounted for by prior achievement and behavioural engagement. Indirect relations from expectancy of success to achievement were moderated by attainment value. Higher attainment value protected performance from low expectancy of success by increasing behavioural engagement. The compensatory role of high attainment value diminished at higher levels of expectancy of success

The dimension of the space of R-places of certain rational function fields

We prove that the space $M(K(x,y))$ of $\mathbb R$-places of the field $K(x,y)$ of rational functions of two variables with coefficients in a totally Archimedean field $K$ has covering and integral dimensions \dim M(K(x,y))=\dim_\IZ M(K(x,y))=2 and the cohomological dimension $\dim_G M(K(x,y))=1$ for any Abelian 2-divisible coefficient group $G$.Comment: 8 page

Preventive medical care in remote Aboriginal communities in the Northern Territory: a follow-up study of the impact of clinical guidelines, computerised recall and reminder systems, and audit and feedback

Background Interventions to improve delivery of preventive medical services have been shown to be effective in North America and the UK. However, there are few studies of the extent to which the impact of such interventions has been sustained, or of the impact of such interventions in disadvantaged populations or remote settings. This paper describes the trends in delivery of preventive medical services following a multifaceted intervention in remote community health centres in the Northern Territory of Australia. Methods The intervention comprised the development and dissemination of best practice guidelines supported by an electronic client register, recall and reminder systems and associated staff training, and audit and feedback. Clinical records in seven community health centres were audited at regular intervals against best practice guidelines over a period of three years, with feedback of audit findings to health centre staff and management. Results Levels of service delivery varied between services and between communities. There was an initial improvement in service levels for most services following the intervention, but improvements were in general not fully sustained over the three year period. Conclusions Improvements in service delivery are consistent with the international experience, although baseline and follow-up levels are in many cases higher than reported for comparable studies in North America and the UK. Sustainability of improvements may be achieved by institutionalisation of relevant work practices and enhanced health centre capacity

Targeting mechanotransduction mechanisms and tissue weakening signals in the human amniotic membrane

Mechanical and inflammatory signals in the fetal membrane play an important role in extracellular matrix (ECM) remodelling in order to dictate the timing of birth. We developed a mechanical model that mimics repetitive stretching of the amniotic membrane (AM) isolated from regions over the placenta (PAM) or cervix (CAM) and examined the effect of cyclic tensile strain (CTS) on mediators involved in mechanotransduction (Cx43, AKT), tissue remodelling (GAGs, elastin, collagen) and inflammation (PGE2, MMPs). In CAM and PAM specimens, the application of CTS increased GAG synthesis, PGE2 release and MMP activity, with concomitant reduction in collagen and elastin content. Co-stimulation with CTS and pharmacological agents that inhibit either Cx43 or AKT, differentially influenced collagen, GAG and elastin in a tissue-dependent manner. SHG confocal imaging of collagen fibres revealed a reduction in SHG intensity after CTS, with regions of disorganisation dependent on tissue location. CTS increased Cx43 and AKT protein and gene expression and the response could be reversed with either CTS, the Cx43 antisense or AKT inhibitor. We demonstrate that targeting Cx43 and AKT prevents strain-induced ECM damage and promotes tissue remodelling mechanisms in the AM. We speculate that a combination of inflammatory and mechanical factors could perturb typical mechanotransduction processes mediated by Cx43 signalling. Cx43 could therefore be a potential therapeutic target to prevent inflammation and preterm premature rupture of the fetal membranes