Lessons learned: rearing the crown-boring weevil, Ceutorhynchus scrobicollis (Coleoptera: Curculionidae), in containment for biological control of garlic mustard (Alliaria petiolata)

In this paper, we describe lessons learned and protocols developed after a decade of rearing Ceutorhynchus scrobicollis Nerenscheimer and Wagner in a Biosafety Level 2 containment facility. We have developed these protocols in anticipation of approval to release C. scrobicollis in North America for the biocontrol of garlic mustard. The rearing protocol tried to minimize the potential of attack by the adult parasitoid, Perilitus conseutor, which may be present in field collected C. scrobicollis from Europe to prevent inadvertent introduction of parasitoids into North America. All C. scrobicollis used for our quarantine rearing were field collected near Berlin, Germany. We have successfully reared C. scrobicollis on caged garlic mustard plants in a growth chamber by alternating temperatures and photoperiods to simulate those in its native range. In Germany, C. scrobicollis produces one generation per year and F1 adults emerge in late May. In containment, a new generation of adults emerged an average of 108 days after adults were placed on plants. We found the optimal time spent to collect F1 adults was four weeks after the appearance of the first F1 adult, with 95% of potential adults collected. Simulating a three-month summer aestivation period, followed by a week of fall, and three weeks of winter conditions resulted in optimum levels of oviposition in F1 females. Larvae first hatched 8- to-10 days after adults were placed on plants at 15/14 C day/night temperatures with a 9.5 hour photoperiod. We therefore recommend that C. scrobicollis adults are removed from garlic mustard rosettes after 8 days. This will maximize the period of female oviposition while minimizing the time when larvae are available for attack from P. conseutor

Constraints on the Universal CIV Mass Density at z~6 from Early IR Spectra Obtained with the Magellan FIRE Spectrograph

We present a new determination of the intergalactic CIV mass density at 4.3 < z < 6.3. Our constraints are derived from high signal-to-noise spectra of seven quasars at z > 5.8 obtained with the newly commissioned FIRE spectrograph on the Magellan Baade telescope, coupled with six observations of northern objects taken from the literature. We confirm the presence of a downturn in the CIV abundance at =5.66 by a factor of 4.1 relative to its value at =4.96, as measured in the same sightlines. In the FIRE sample, a strong system previously reported in the literature as CIV at z=5.82 is re-identified as MgII at z=2.78, leading to a substantial downward revision in $\Omega_{CIV}$ for these prior studies. Additionally we confirm the presence of at least two systems with low-ionization CII, SiII, and OI absorption but relatively weak signal from CIV. The latter systems systems may be of interest if the downward trend in $\Omega_{CIV}$ at high redshift is driven in part by ionization effects.Comment: 14 pages, 6 figures, 2 tables. Submitted to Ap

Lessons learned: rearing the crown-boring weevil, Ceutorhynchus scrobicollis (Coleoptera: Curculionidae), in containment for biological control of garlic mustard (Alliaria petiolata)

In this paper, we describe lessons learned and protocols developed after a decade of rearing Ceutorhynchus scrobicollis Nerenscheimer and Wagner in a Biosafety Level 2 containment facility. We have developed these protocols in anticipation of approval to release C. scrobicollis in North America for the biocontrol of garlic mustard. The rearing protocol tried to minimize the potential of attack by the adult parasitoid, Perilitus conseutor, which may be present in field collected C. scrobicollis from Europe to prevent inadvertent introduction of parasitoids into North America. All C. scrobicollis used for our quarantine rearing were field collected near Berlin, Germany. We have successfully reared C. scrobicollis on caged garlic mustard plants in a growth chamber by alternating temperatures and photoperiods to simulate those in its native range. In Germany, C. scrobicollis produces one generation per year and F1 adults emerge in late May. In containment, a new generation of adults emerged an average of 108 days after adults were placed on plants. We found the optimal time spent to collect F1 adults was four weeks after the appearance of the first F1 adult, with 95% of potential adults collected. Simulating a three-month summer aestivation period, followed by a week of fall, and three weeks of winter conditions resulted in optimum levels of oviposition in F1 females. Larvae first hatched 8- to-10 days after adults were placed on plants at 15/14 C day/night temperatures with a 9.5 hour photoperiod. We therefore recommend that C. scrobicollis adults are removed from garlic mustard rosettes after 8 days. This will maximize the period of female oviposition while minimizing the time when larvae are available for attack from P. conseutor

Forming Young Bulges within Existing Disks: Statistical Evidence for External Drivers

Contrary to traditional models of galaxy formation, recent observations suggest that some bulges form within preexisting disk galaxies. Such late-epoch bulge formation within disks seems to be linked to disk gas inflow and central star formation, caused by either internal secular processes or galaxy mergers and interactions. We identify a population of galaxies likely to be experiencing active bulge growth within disks, using the criterion that the color within the half-light radius is bluer than the outer disk color. Such blue-centered galaxies make up >10% of star-forming disk galaxies within the Nearby Field Galaxy Survey, a broad survey designed to represent the natural diversity of the low-z galaxy population over a wide range of luminosities and environments. Blue-centered galaxies correlate at 99% confidence with morphological peculiarities suggestive of minor mergers and interactions. From this and other evidence, we argue that external drivers rather than internal secular processes probably account for the majority of blue-centered galaxies. We go on to discuss quantitative plausibility arguments indicating that blue-centered evolutionary phases may represent an important mode of bulge growth for most disk galaxies, leading to significant changes in bulge-to-disk ratio without destroying disks. If this view is correct, bulge growth within disks may be a natural consequence of the repeated galaxy mergers and interactions inherent in hierarchical galaxy formation.Comment: 18 pages including 12 figures, AJ, accepte

Concentrations of Polychlorinated Biphenyls (PCB’s), Chlorinated Pesticides, and Heavy Metals and Other Elements in Tissues of Belugas, Delphinapterus leucas, from Cook Inlet

Tissues from Cook Inlet beluga whales, Delphinapterus leucas, that were collected as part of the Alaska Marine Mammal Tissue Archival Project were analyzed for polychlorinated biphenyls (PCB’s), chlorinated pesticides, and heavy metals and other elements. Concentrations of total PCB’s (ΣPCB’s), total DDT (ΣDDT), chlordane compounds, hexachlorobenzene (HCB), dieldrin, mirex, toxaphene, and hexachlorocyclohexane (HCH) measured in Cook Inlet beluga blubber were compared with those reported for belugas from two Arctic Alaska locations (Point Hope and Point Lay), Greenland, Arctic Canada, and the highly contaminated stock from the St. Lawrence estuary in eastern Canada. The Arctic and Cook Inlet belugas had much lower concentrations (ΣPCB’s and ΣDDT were an order of magnitude lower) than those found in animals from the St. Lawrence estuary. The Cook Inlet belugas had the lowest concentrations of all (ΣPCB’s aver-aged 1.49 ± 0.70 and 0.79 ± 0.56 mg/kg wet mass, and ΣDDT averaged 1.35 ± 0.73 and 0.59 ± 0.45 mg/kg in males and females, respectively). Concentrations in the blubber of the Cook Inlet males were significantly lower than those found in the males of the Arctic Alaska belugas (ΣPCB’s and ΣDDT were about half). The lower levels in the Cook Inlet animals might be due to differences in contaminant sources, food web differences, or different age distributions among the animals sampled. Cook Inlet males had higher mean and median concentrations than did females, a result attributable to the transfer of these compounds from mother to calf during pregnancy and during lactation. Liver concentrations of cadmium and mercury were lower in the Cook Inlet belugas (most cadmium values were <1 mg/kg and mercury values were 0.704–11.42 mg/kg wet mass), but copper levels were significantly higher in the Cook Inlet animals (3.97–123.8 mg/kg wet mass) than in Arctic Alaska animals and similar to those reported for belugas from Hudson Bay. Although total mercury levels were the lowest in the Cook Inlet population, methylmercury concentrations were similar among all three groups of the Alaska animals examined (0.34–2.11 mg/kg wet mass). As has been reported for the Point Hope and Point Lay belugas, hepatic concentrations of silver were r

Comparing knowledge, accessibility, and use of evidence-based chronic disease prevention processes across four countries

<p>Background: Evidence-based chronic disease prevention (EBCDP) effectively reduces incidence rates of many chronic diseases, but contextual factors influence the implementation of EBCDP worldwide. This study aims to examine the following contextual factors across four countries: knowledge, access, and use of chronic disease prevention processes.</p><p>Methods: In this cross-sectional study, public health practitioners (N = 400) from Australia (n = 121), Brazil (n = 76), China (n = 102), and the United States (n = 101) completed a 26-question survey on EBCDP. One-way ANOVA and Pearson's Chi-Square tests were used to assess differences in contextual factors of interest by country.</p><p>Results: Practitioners in China reported less knowledge of EBCDP processes (p < 0.001) and less use of repositories of evidence-based interventions, than those from other countries (p < 0.001). Academic journals were the most frequently used method for accessing information about evidence-based interventions across countries. When selecting interventions, Brazilian and Chinese practitioners were more likely to consider implementation ease while the Australian and United States practitioners were more likely to consider effectiveness (p < 0.001).</p><p>Conclusions: These findings can help inform and improve within and across country strategies for implementing EBCDP interventions.</p

Estimating uncertainty in density surface models

This work was funded by OPNAV N45 and the SURTASS LFA Settlement Agreement, and being managed by the U.S. Navy’s Living Marine Resources program under Contract No. N39430-17-C-1982.Providing uncertainty estimates for predictions derived from species distribution models is essential for management but there is little guidance on potential sources of uncertainty in predictions and how best to combine these. Here we show where uncertainty can arise in density surface models (a multi-stage spatial modelling approach for distance sampling data), focussing on cetacean density modelling. We propose an extensible, modular, hybrid analytical-simulation approach to encapsulate these sources. We provide example analyses of fin whales Balaenoptera physalus in the California Current Ecosystem.Publisher PDFPeer reviewe

Law Enforcement Preferences for PTSD Treatment and Crisis Management Alternatives

Evidence-based treatments (EBT) for posttraumatic stress disorder (PTSD) remain underutilized. Analog research, however, indicates that patients may be more amenable to receiving EBT for PTSD than utilization rates suggest. This study sought to extend previous studies by investigating PTSD treatment preferences among law enforcement individuals (i.e., active duty officers, cadets, criminal justice students). We asked 379 participants, with varying trauma histories, to read a police traumatic event and imagine they had developed PTSD. Participants rated the credibility of six treatment options which they might encounter in a treatment setting, and chose their most and least preferred treatments. Next, they evaluated a widely used debriefing intervention aimed at preventing PTSD. Almost 90% of participants chose exposure or cognitive processing therapy as their first or second most preferred treatment, and they rated these interventions as significantly more credible than the other 4 treatment options. The sample showed ambivalence regarding the perceived efficacy of debriefing but found the rationale credible. This study supports previous analog research indicating that patients may be more interested EBT than indicated by utilization rates, and suggests that law enforcement departments should consider offering EBT to officers who develop PTSD

A Neural Region of Abstract Working Memory

■ Over 350 years ago, Descartes proposed that the neural basis of consciousness must be a brain region in which sen-sory inputs are combined. Using fMRI, we identified at least one such area for working memory, the limited information held in mind, described by William James as the trailing edge of consciousness. Specifically, a region in the left intraparietal sulcus was found to demonstrate load-dependent activity for either visual stimuli (colored squares) or a combination of vi-sual and auditory stimuli (spoken letters). This result was repli-cated across two experiments with different participants and methods. The results suggest that this brain region, previously well known for working memory of visually presented materials, actually holds or refers to information from more than one modality.