Detection and mapping of Cannabinoids in single hair samples through rapid derivatization- Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

The sample preparation method reported in this work has permitted for the first time the application of Matrix Assisted Laser Desorption Ionization Mass Spectrometry Profiling and Imaging (MALDI-MSP and MALDI-MSI) for the detection and mapping of cannabinoids in a single hair sample. MALDI-MSI analysis of hair samples has recently been suggested as an alternative technique to traditional methods of GC-MS and LC-MS due to simpler sample preparation, the ability to detect a narrower time frame of drug use and a reduction in sample amount required. However, despite cannabis being the most commonly used illicit drug worldwide, a MALDI-MS method for the detection and mapping of cannabinoids in a single hair has not been reported. This is probably due to the poor ionization efficiency of the drug and its metabolites and low concentration incorporated into hair. This research showed that the in situ derivatization of cannabinoids through addition of an N-methylpyridium group resulted in improved ionization efficiency, permitting both detection and mapping of Δ9-tetrahydrocannabinol (THC), Cannabinol (CBN), cannabidiol (CBD) and the metabolites 11-nor-9-carboxy-tetrahydrocannabinol (THC-COOH), 11-Hydroxy-delta-9-tetrahydrocannabinol (11-OH-THC) and 11-nor-delta(9)-carboxy-tetrahydrocannabinol glucuronide (THC-COO-glu). Additionally, for the first time an in-source re-arrangement of THC was observed and characterised in this paper thus contributing to new and accurate knowledge in the analysis of this drug by MALDI mass spectrometry

Expanded risk groups help determine which prostate radiotherapy sub-group may benefit from adjuvant androgen deprivation therapy

<p>Abstract</p> <p>Purpose</p> <p>To assess whether an expanded (five level) risk stratification system can be used to identify the sub-group of intermediate risk patients with prostate cancer who benefit from combining androgen deprivation therapy (ADT) with external beam radiotherapy (EBRT).</p> <p>Materials and methods</p> <p>Using a previously validated 5-risk group schema, a prospective non-randomized data set of 1423 men treated at the British Columbia Cancer Agency was assessed for the primary end point of biochemical control (bNED) with the RTOG-ASTRO "Phoenix" definition (lowest PSA to date + 2 ng/mL), both with and without adjuvant ADT. The median follow-up was 5 years.</p> <p>Results</p> <p>There was no bNED benefit for ADT in the low or low intermediate groups but there was a statistically significant bNED benefit in the high intermediate, high and extreme risk groups. The 5-year bNED rates with and without ADT were 70% and 73% respectively for the low intermediate group (p = non-significant) and 72% and 58% respectively for the high intermediate group (p = 0.002).</p> <p>Conclusion</p> <p>There appears to be no advantage to ADT where the Gleason score is 6 or less and PSA is 15 or less. ADT is beneficial in patients treated to standard dose radiation with Gleason 6 disease and a PSA greater than 15 or where the Gleason score is 7 or higher.</p

Distributions of nuclear fuel-reprocessing tracers in the Arctic Ocean: Indications of Russian river influence

Radionuclide sampling in 1986 and 1993 in the Canada Basin, and in 1993 in the Amundsen Basin and on the adjacent Laptev shelf, provides new insights into the origin, timing, pathways, and mechanisms for dispersal of non-fallout radioactive tracers in the Arctic Ocean. First, samples from the Beaufort Sea shelf, slope, and adjacent basin show a four-fold increase in 129I concentrations from 1986 to 1993. Second, anthropogenic non-fallout radionuclide concentrations in the Beaufort Sea increase with proximity to slope boundary currents. Third, there is evidence for riverine contributions of anthropogenic radionuclides to surface waters of the Amundsen Basin and the Laptev continental shelf. This evidence includes high surface water burdens of 237Np and 129I, with the maximum in anthropogenic 129I found in the least saline and most 18O-depleted waters, consistent with an origin in high-latitude runoff. Additionally, the 237Np/129I atom ratios in the Laptev Sea and Amundsen Basin in 1993 were significantly lower than observed elsewhere in the Arctic Ocean and can be reasonably explained by 129I added during transit of the Russian shelves. The 240Pu/239Pu ratios in the water column were mostly near 0.18, consistent both with stratospheric bomb fallout and with the discharged-weighted mean Sellafield ratio during 1966-1985. In the least saline water samples collected at the most shallow Laptev shelf station, however, the Pu ratios were lower, consistent with a non-European nuclear fuel reprocessing source. There are clear secondary maxima in 237Np and 129I near 1000 m in the Amundsen Basin, likely associated with the Barents Sea branch of Atlantic water. Finally, the 129I/salinity and 129I/δ18O relationships in the Amundsen and Canada Basins at middepths are indistinguishable, suggesting effective horizontal dispersion

Probing the Intermediate-Age Globular Clusters in NGC 5128 from Ultraviolet Observations

We explore the age distribution of the globular cluster (GC) system of the nearby elliptical galaxy NGC 5128 using ultraviolet (UV) photometry from Galaxy Evolution Explorer (GALEX) observations, with UV - optical colors used as the age indicator. Most GCs in NGC 5128 follow the general trends of GCs in M31 and Milky Way in UV - optical color-color diagram, which indicates that the majority of GCs in NGC 5128 are old similar to the age range of old GCs in M31 and Milky Way. A large fraction of spectroscopically identified intermediate-age GC (IAGC) candidates with ~ 3-8 Gyr are not detected in the FUV passband. Considering the nature of intermediate-age populations being faint in the far-UV (FUV) passband, we suggest that many of the spectroscopically identified IAGCs may be truly intermediate in age. This is in contrast to the case of M31 where a large fraction of spectroscopically suggested IAGCs are detected in FUV and therefore may not be genuine IAGCs but rather older GCs with developed blue horizontal branch stars. Our UV photometry strengthens the results previously suggesting the presence of GC and stellar subpopulation with intermediate age in NGC 5128. The existence of IAGCs strongly indicates the occurrence of at least one more major star formation episode after a starburst at high redshift.Comment: 8 pages, 3 figures, accepted for ApJ Lette

Globular cluster systems in low-luminosity early-type galaxies near the Fornax Cluster centre

We present a photometric study of the globular cluster systems of the Fornax cluster galaxies NGC 1374, NGC 1379, and NGC 1387. The data consists of images from the wide-field MOSAIC Imager of the CTIO 4-m telescope, obtained with Washington C and Kron-Cousins R filters. The images cover a field of 36 x 36 arcmin, corresponding to 200 x 200 kpc at the Fornax distance. Two of the galaxies, NGC 1374 and NGC 1379, are low-luminosity ellipticals while NGC 1387 is a low-luminosity lenticular. Their cluster systems are still embedded in the cluster system of NGC 1399. Therefore the use of a large field is crucial and some differences to previous work can be explained by this. The colour distributions of all globular cluster systems are bimodal. NGC 1387 presents a particularly distinct separation between red and blue clusters and an overproportionally large population of red clusters. The radial distribution is different for blue and red clusters, red clusters being more concentrated towards the respective galaxies. The different colour and radial distributions point to the existence of two globular cluster subpopulations in these galaxies. Specific frequencies are in the range S_N= 1.4-2.4, smaller than the typical values for elliptical galaxies. These galaxies might have suffered tidal stripping of blue globular clusters by NGC 1399.Comment: 14 pages, 12 figures. Accepted for publication in MNRA

Chikungunya Virus and Central Nervous System Infections in Children, India

Chikungunya virus (CHIKV) is a mosquito-borne alphavirus best known for causing fever, rash, arthralgia, and occasional neurologic disease. By using real-time reverse transcription–PCR, we detected CHIKV in plasma samples of 8 (14%) of 58 children with suspected central nervous system infection in Bellary, India. CHIKV was also detected in the cerebrospinal fluid of 3 children