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Omega 3 Fatty Acid Inhibition of Inflammatory Cytokine-Mediated Connexin43 Regulation in the Heart
Background: The proinflammatory cytokine , which increases in the heart post myocardial infarction (MI), has been shown to cause loss of Connexin43 (Cx43) function, an event known to underlie formation of the arrhythmogenic substrate. Omega 3 Fatty acids exhibit antiarrhythmic properties and impact signaling. We hypothesize that Omega-3 fatty acids prevent arrhythmias in part, by inhibiting signaling thus maintaining functional Cx43 channels. Methods: Rat neonatal myocytes or Madin-Darby Canine Kidney Epithelial (MDCK) cells grown in media in the absence (Ctr) or presence of docosahexaenoic acid (DHA, an Omega-3 Fatty acid) were treated with activated . We determined Cx43 channel function using a dye spread assay. Western blot and immunostaining were used to examine Cx43 levels/localization and downstream effectors of . In addition we used a murine model of MI for 24 h to determine the impact of an Omega-3 fatty acid enriched diet on Cx43 levels/localization post MI. Results: significantly inhibited Cx43 function in Ctr cells . However, DHA-treated cells remained highly coupled in the presence of . Additionally, western blot showed that treatment caused a 38.5% downregulation of Cx43 which was completely abolished in DHA-treated cells . Examination of the downstream modulator of , showed that while hypoxia caused translocation of to the nucleus, this was inhibited by DHA. Additionally we found that a diet enriched in Omega-3 Fatty acids inhibited lateralization of Cx43 in the post-MI murine heart as well as limited activation of fibroblasts which would lead to decreased fibrosis overall. Conclusions: Omega 3 Fatty acid treatment inhibited -stimulated loss of Cx43 protein, and more importantly, inhibited loss of Cx43 function by inhibiting translocation of . In the intact heart a diet enriched in Omega 3 Fatty Acids limited loss of Cx43 at the intercalated disk in the heart following MI. These data suggest that one of cardio-protective mechanisms by which Omega 3 Fatty acids work includes prevention of the pro-arrhythmic loss of Cx43 post MI and the attenuation of cardiac fibrosis after injury
Differences in reef fish assemblages between populated and remote reefs spanning multiple archipelagos across the central and western Pacific
Comparable information on the status of natural resources across large geographic and human impact scales provides invaluable context to ecosystem-based management and insights into processes driving differences among areas. Data on fish assemblages at 39 US flag coral reef-areas distributed across the Pacific are presented. Total reef fish biomass varied by more than an order of magnitude: lowest at densely-populated islands and highest on reefs distant from human populations. Remote reefs (<50 people within 100 km) averaged ~4 times the biomass of “all fishes” and 15 times the biomass of piscivores compared to reefs near populated areas. Greatest within-archipelagic differences were found in Hawaiian and Mariana Archipelagos, where differences were consistent with, but likely not exclusively driven by, higher fishing pressure around populated areas. Results highlight the importance of the extremely remote reefs now contained within the system of Pacific Marine National Monuments as ecological reference areas
HST/STIS Spectroscopy of the Lyman-Alpha Emission Line in the Central Dominant Galaxies in A426, A1795, and A2597: Constraints on Clouds in the Intracluster Medium
We report on HST/STIS spectra of the Lyman-alpha emission in the central
dominant galaxies in three rich clusters of galaxies. We find evidence for a
population of clouds in the intracluster medium.We detect 10 Ly-alpha
absorption systems towards the nucleus of NGC1275 with columns of N(HI)
1E12-1E14 cm-2. The detected absorption features are most consistent with
associated nuclear absorption systems. There is very little nuclear absorption
at the systemic velocity in NGC1275. This implies that the large columns
detected in the 21 cm line towards the parsec scale radio source avoid the line
of sight to the nucleus. This gas may be located in a circumnuclear disk or
torus. We detect at least one and possibly two absorption features towards the
extended Ly-alpha in A426. We do not detect absorption towards the extended
Ly-alpha emission in A1795, and A2597 with upper limits N(HI) 1E13 cm-2 for
optically thin absorbers. Our data constrain the covering factor of any high
column density gas in the ICM to be less than 25%. Our results suggest that the
lack of observed intermediate temperature gas is not explained by obscuration.
In addition, the low columns of gas on the 100 kpc scales in the ICM suggests
that (1) the rate at which cold gas accumulates in the ICM on these scales is
very low, and (2) the dense nebulae in the central 10 kpc must have cooled or
been deposited in situ.Comment: 6 figure
The Hubble Deep Field South Flanking Fields
As part of the Hubble Deep Field South program, a set of shorter 2-orbit
observations were obtained of the area adjacent to the deep fields. The WFPC2
flanking fields cover a contiguous solid angle of 48 square arcminutes.
Parallel observations with the STIS and NICMOS instruments produce a patchwork
of additional fields with optical and near-infrared (1.6 micron) response.
Deeper parallel exposures with WFPC2 and NICMOS were obtained when STIS
observed the NICMOS deep field. These deeper fields are offset from the rest,
and an extended low surface brightness object is visible in the deeper WFPC2
flanking field. In this data paper, which serves as an archival record of the
project, we discuss the observations and data reduction, and present SExtractor
source catalogs and number counts derived from the data. Number counts are
broadly consistent with previous surveys from both ground and space. Among
other things, these flanking field observations are useful for defining slit
masks for spectroscopic follow-up over a wider area around the deep fields, for
studying large-scale structure that extends beyond the deep fields, for future
supernova searches, and for number counts and morphological studies, but their
ultimate utility will be defined by the astronomical community.Comment: 46 pages, 15 figures. Images and full catalogs available via the
HDF-S at http://www.stsci.edu/ftp/science/hdfsouth/hdfs.html at present. The
paper is accepted for the February 2003 Astronomical Journal. Full versions
of the catalogs will also be available on-line from AJ after publicatio
The STIS Parallel Survey: Introduction and First Results
The installation of the Space Telescope Imaging Spectrograph (STIS) on the
Hubble Space Telescope (HST) allows for the first time two-dimensional optical
and ultraviolet slitless spectroscopy of faint objects from space. The STIS
Parallel Survey (SPS) routinely obtains broad band images and slitless spectra
of random fields in parallel with HST observations using other instruments. The
SPS is designed to study a wide variety of astrophysical phenomena, including
the rate of star formation in galaxies at intermediate to high redshift through
the detection of emission-line galaxies. We present the first results of the
SPS, which demonstrate the capability of STIS slitless spectroscopy to detect
and identify high-redshift galaxies.Comment: 11 pages, Latex, 3 enclosed Postscript figures, aaspp4.sty, accepted
for publication in the Astrophysical Journal Letters HST Second Servicing
Mission special issu
Pet Rodents and Fatal Lymphocytic Choriomeningitis in Transplant Patients
A unique strain of this virus was traced back to hamsters from an Ohio rodent distribution facility
The association between misperceptions around weight status and quality of life in adults in Australia
Objective: Limited evidence supports a possible association between a person’s perception of their weight status and their quality of life (QoL). This study evaluates whether misperception around weight status is associated with QoL and the impact of gender on this association. Methods: A cross-sectional survey of Australian adults (n=1,905 analysed) collected selfreported height and weight (used to estimate BMI), gender and QoL (described using the AQoL-8D). Participants reported whether they perceived their weight status to be ‘underweight’, ‘healthy weight’, ‘overweight’ or ‘obese’. Misperception around weight status was categorised based on perceived weight status and self-reported BMI. Ordinary least squares regression was used to test associations between self-reported overall, physical and psychosocial QoL, misperception of weight status, and gender, across different BMI categories, after controlling for income, education, relationship status and health conditions. Results: Compared to accurate perception, underestimation of weight status was associated with higher overall QoL for obese males and females and for overweight males. Overestimation of weight status was associated with higher overall QoL for underweight females and lower overall QoL for healthy weight males and females. The same pattern was seen for psychosocial QoL. Physical QoL was less sensitive to misperception than psychosocial QoL. Conclusions: Self-reported misperception around weight status is associated with overall, psychosocial and to a lesser extent physical QoL in Australian adults, although its role depends on BMI category and gender. Generally misperception in the direction of “healthy weight” is associated with higher QoL and overestimation of weight status by those who are of healthy weight is associated with lower QoL. Findings should be confirmed in datasets that contain measured as opposed to self-report height and weight
Functional significance of the hemadsorption activity of influenza virus neuraminidase and its alteration in pandemic viruses
Human influenza viruses derive their genes from avian viruses. The neuraminidase (NA) of the avian viruses has, in addition to the catalytic site, a separate sialic acid binding site (hemadsorption site) that is not present in human viruses. The biological significance of the NA hemadsorption activity in avian influenza viruses remained elusive. A sequence database analysis revealed that the NAs of the majority of human H2N2 viruses isolated during the influenza pandemic of 1957 differ from their putative avian precursor by amino acid substitutions in the hemadsorption site. We found that the NA of a representative pandemic virus A/Singapore/1/57 (H2N2) lacks hemadsorption activity and that a single reversion to the avian-virus-like sequence (N367S) restores hemadsorption. Using this hemadsorption-positive NA, we generated three NA variants with substitutions S370L, N400S and W403R that have been found in the hemadsorption site of human H2N2 viruses. Each substitution abolished hemadsorption activity. Although, there was no correlation between hemadsorption activity of the NA variants and their enzymatic activity with respect to monovalent substrates, all four hemadsorption-negative NAs desialylated macromolecular substrates significantly slower than did the hemadsorption-positive counterpart. The NA of the 1918 pandemic virus A/Brevig Mission/1/18 (H1N1) also differed from avian N1 NAs by reduced hemadsorption activity and less efficient hydrolysis of macromolecular substrates. Our data indicate that the hemadsorption site serves to enhance the catalytic efficiency of NA and they suggest that, in addition to changes in the receptor-binding specificity of the hemagglutinin, alterations of the NA are needed for the emergence of pandemic influenza viruses
How Do Police Respond to Stalking? An Examination of the Risk Management Strategies and Tactics Used in a Specialized Anti-Stalking Law Enforcement Unit
How do police respond to and manage complaints of stalking? To answer this question, we conducted a 3-phase study. First, we reviewed the literature to identify risk management tactics used to combat stalking. Second, we asked a group of police officers to review those tactics for completeness and group them into categories reflecting more general risk management strategies. The result was 22 categories of strategies. Finally, we used qualitative methods to evaluate the files of 32 cases referred to the specialized anti-stalking unit of a metropolitan police department. We coded specific risk management tactics and strategies used by police. Results indicated that a median number of 19 specific tactics from 7 general strategies were used to manage risk. Also, the implementation of strategies and tactics reflected specific characteristics of the cases (e.g., perpetrator risk factors, victim vulnerability factors), suggesting that the risk management decisions made by police were indeed strategic in nature. Qualitative analyses indicated that some of the strategies and tactics were more effective than others. We discuss how these findings can be used to understand and use stalking risk management more generally, as well as improve research on the efficacy of risk assessment and management for stalking
Evaluating the Potential Effectiveness of Compensatory Mitigation Strategies for Marine Bycatch
Conservationists are continually seeking new strategies to reverse population declines and safeguard against species extinctions. Here we evaluate the potential efficacy of a recently proposed approach to offset a major anthropogenic threat to many marine vertebrates: incidental bycatch in commercial fisheries operations. This new approach, compensatory mitigation for marine bycatch (CMMB), is conceived as a way to replace or reduce mandated restrictions on fishing activities with compensatory activities (e.g., removal of introduced predators from islands) funded by levies placed on fishers. While efforts are underway to bring CMMB into policy discussions, to date there has not been a detailed evaluation of CMMB's potential as a conservation tool, and in particular, a list of necessary and sufficient criteria that CMMB must meet to be an effective conservation strategy. Here we present a list of criteria to assess CMMB that are tied to critical ecological aspects of the species targeted for conservation, the range of possible mitigation activities, and the multi-species impact of fisheries bycatch. We conclude that, overall, CMMB has little potential for benefit and a substantial potential for harm if implemented to solve most fisheries bycatch problems. In particular, CMMB is likely to be effective only when applied to short-lived and highly-fecund species (not the characteristics of most bycatch-impacted species) and to fisheries that take few non-target species, and especially few non-seabird species (not the characteristics of most fisheries). Thus, CMMB appears to have limited application and should only be implemented after rigorous appraisal on a case-specific basis; otherwise it has the potential to accelerate declines of marine species currently threatened by fisheries bycatch
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