Evolutionary consequences of feedbacks between within-host competition and disease control

Lay Summary: Competition often occurs among diverse parasites within a single host, but control efforts could change its strength. We examined how the interplay between competition and control could shape the evolution of parasite traits like drug resistance and disease severity

Data from: Trade‐off between reproductive and anti‐competitor abilities in an insect–parasitic nematode–bacteria symbiosis

Mutualistic symbionts can provide diverse benefits to their hosts and often supply key trait variation for host adaptation. The bacterial symbionts of entomopathogenic nematodes play a crucial role in successful colonization of and reproduction in the insect host. Additionally, these symbionts can produce a diverse array of antimicrobial compounds to deter within‐host competitors. Natural isolates of the symbiont, Xenorhabdus bovienii, show considerable variation in their ability to target sympatric competitors via bacteriocins, which can inhibit the growth of sensitive Xenorhabdus strains. Both the bacteria and its nematode partner have been shown to benefit from bacteriocin production when within‐host competition with a sensitive competitor occurs. Despite this benefit, several isolates of Xenorhabdus do not inhibit sympatric strains. To understand how this variation in allelopathy could be maintained, we tested the hypothesis that inhibiting isolates face a reproductive cost in the absence of competition. We tested this hypothesis by examining the reproductive success of inhibiting and non‐inhibiting isolates coupled with their natural nematode host in a non‐competitive context. We found that nematodes carrying non‐inhibitors killed the insect host more rapidly and were more likely to successfully reproduce than nematodes carrying inhibitors. Lower reproductive success of inhibiting isolates was repeatable across nematode generations and across insect host species. However, no difference in insect mortality was observed between inhibiting and non‐inhibiting isolates when bacteria were injected into insects without their nematode partners. Our results indicate a trade‐off between the competitive and reproductive roles of symbionts, such that inhibiting isolates, which are better in the face of within‐host competition, pay a reproductive cost in the absence of competition. Furthermore, our results support the hypothesis that symbiont variation within populations can be maintained through context‐dependent fitness benefits conferred to their hosts. As such, our study offers novel insights into the selective forces maintaining variation within a single host–symbiont population and highlights the role of competition in mutualism evolution

Data and SAS code for Figures 2 and 3

First worksheet holds data, 2nd definitions, 3rd SAS cod

Data and SAS code for Figure 4

First worksheet holds data, 2nd definitions, 3rd SAS code used in paper

CostOfResistance Analyses

CostOfResistance Analyse

Data from: Suppression of bacteriocin resistance using live, heterospecific competitors

Rapidly spreading antibiotic resistance has led to the need for novel alternatives and sustainable strategies for antimicrobial use. Bacteriocins are a class of proteinaceous anticompetitor toxins under consideration as novel therapeutic agents. However, bacteriocins, like other antimicrobial agents, are susceptible to resistance evolution, and will require the development of sustainable strategies to prevent or decelerate the evolution of resistance. Here we conduct proof-of-concept experiments to test whether introducing a live, heterospecific competitor along with a bacteriocin dose can effectively suppress the emergence of bacteriocin resistance in vitro. Previous work with conventional chemotherapeutic agents suggests that competition between conspecific sensitive and resistant pathogenic cells can effectively suppress the emergence of resistance in pathogenic populations. However, the threshold of sensitive cells required for such competitive suppression of resistance may often be too high to maintain host health. Therefore, here we aim to ask whether the principle of competitive suppression can be effective if a heterospecific competitor is used. Our results show that a live competitor introduced in conjunction with low bacteriocin dose can effectively control resistance and suppress sensitive cells. Further, this efficacy can be matched by using a bacteriocin-producing competitor without any additional bacteriocin. These results provide strong proof-of-concept for the effectiveness of competitive suppression using live, heterospecific competitors. Currently in-use probiotic strains or commensals may provide promising candidates for the therapeutic use of bacteriocin-mediated competitive suppression

Data and SAS code for Figure 5

First worksheet holds data, 2nd definitions, 3rd SAS cod

Figure 1 Data and code

Figure 1 Data and cod

Figure 4 Data and Code

Figure 4 Data and Cod

Figure S1 Analysis

SAS code used to analyze data in Supplemental Figure S1