Kritische Diskursanalyse : Darstellung anhand der Analyse der Nahostberichterstattung linker Medien

Zuerst erschienen im Dietz-Verlag: Bartel, Daniel; Ullrich, Peter; Ehrlich, Kornelia: Kritische Diskursanalyse : Darstellung anhand der Analyse der Nahostberichterstattung linker Medien. - In: Freikamp, Ulrike [u.a.] (Hrsg.): Kritik mit Methode? : Forschungsmethoden und Gesellschaftskritik. - Berlin: Dietz, 2008. - (Texte / Rosa-Luxemburg-Stiftung; 42). - ISBN: 978-3-320-02136-8. - S. 53–72

Diskriminierung als Thema in der migrationsbezogenen Beratung in Sachsen

(Rassistische) Diskriminierung ist eine gesellschaftliche Realität, mit der Migrant_innen in besonderem Maße konfrontiert sind – sei es bei der Arbeits- oder Wohnungssuche, im Kontakt mit Behörden, beim Zugang zu Bildung oder dem Abschluss eines Kaufvertrages. Damit ist das Thema Diskriminierung zugleich eine Herausforderung für die Beratungsarbeit in Bereichen mit Migrationsbezug. Im Rahmen des Projektes „Diskriminierung – (k)ein Thema der migrationsbezogenen Beratung?“ haben wir Berater_innen interviewt und eine Onlinebefragung durchgeführt, um einen besseren Einblick in die gegenwärtige Praxis, die konkreten Herausforderungen und gelingende Beispiele im beraterischen Umgang mit Diskriminierung und Diskriminierungserfahrungen zu bekommen. Insgesamt haben 25 Berater_innen an den Interviews teilgenommen und 66 Personen den Fragebogen ausgefüllt. Die Befragten arbeiten in verschiedenen Orten und Regionen Sachsens in den Bereichen Jugendmigrationsberatung (JMD), Migrationsberatung für Erwachsene (MBE), Sozialarbeit im Bereich Flucht / Asyl sowie Arbeitsmarkt- und Anerkennungsberatung für Migrant_innen. Die Ergebnisse dieser Gespräche und Befragungen haben wir im Rahmen von drei Fachtagen zur Diskussion gestellt und vertieft. Redaktionsschluss: Dezember 201

BIOAGE

BIOAGE se dedica a investigar, comercializar y distribuir soluciones para las personas interesadas en su bienestar y salud ofreciendo un análisis, un informe personalizado y un tratamiento continuado en el tiempo

Proteome analysis of vaccinia virus IHD-W-infected HEK 293 cells with 2-dimensional gel electrophoresis and MALDI-PSD-TOF MS of on solid phase support N-terminally sulfonated peptides

<p>Abstract</p> <p>Background</p> <p>Despite the successful eradication of smallpox by the WHO-led vaccination programme, pox virus infections remain a considerable health threat. The possible use of smallpox as a bioterrorism agent as well as the continuous occurrence of zoonotic pox virus infections document the relevance to deepen the understanding for virus host interactions. Since the permissiveness of pox infections is independent of hosts surface receptors, but correlates with the ability of the virus to infiltrate the antiviral host response, it directly depends on the hosts proteome set. In this report the proteome of HEK293 cells infected with Vaccinia Virus strain IHD-W was analyzed by 2-dimensional gel electrophoresis and MALDI-PSD-TOF MS in a bottom-up approach.</p> <p>Results</p> <p>The cellular and viral proteomes of VACV IHD-W infected HEK293 cells, UV-inactivated VACV IHD-W-treated as well as non-infected cells were compared. Derivatization of peptides with 4-sulfophenyl isothiocyanate (SPITC) carried out on ZipTipμ-C18 columns enabled protein identification via the peptides' primary sequence, providing improved s/n ratios as well as signal intensities of the PSD spectra. The expression of more than 24 human proteins was modulated by the viral infection. Effects of UV-inactivated and infectious viruses on the hosts' proteome concerning energy metabolism and proteins associated with gene expression and protein-biosynthesis were quite similar. These effects might therefore be attributed to virus entry and virion proteins. However, the modulation of proteins involved in apoptosis was clearly correlated to infectious viruses.</p> <p>Conclusions</p> <p>The proteome analysis of infected cells provides insight into apoptosis modulation, regulation of cellular gene expression and the regulation of energy metabolism. The confidence of protein identifications was clearly improved by the peptides' derivatization with SPITC on a solid phase support. Some of the identified proteins have not been described in the context of poxvirus infections before and need to be further characterised to identify their meaning for apoptosis modulation and pathogenesis.</p

Colourfulness as a possible measure of object proximity in the larval zebrafish brain

The encoding of light increments and decrements by separate On- and Off- systems is a fundamental ingredient of vision, which supports edge detection and makes efficient use of the limited dynamic range of visual neurons. Theory predicts that the neural representation of On- and Off-signals should be balanced, including across an animal’s visible spectrum. Here we find that larval zebrafish violate this textbook expectation: in the zebrafish brain, UV-stimulation near exclusively gives On-responses, blue/green stimulation mostly Off-responses, and red-light alone elicits approximately balanced On- and Off-responses (see also references2, 3, 4). We link these findings to zebrafish visual ecology, and suggest that the observed spectral tuning boosts the encoding of object ‘colourfulness’, which correlates with object proximity in their underwater world

Molecular cloning and characterization of an amidase from Arabidopsis thaliana capable of converting indole-3-acetamide into the plant growth hormone, indole-3-acetic acid

Acylamidohydrolases from higher plants have not been characterized or cloned so far. AtAMI1 is the first member of this enzyme family from a higher plant and was identified in the genome of Arabidopsis thaliana based on sequence homology with the catalytic-domain sequence of bacterial acylamidohydrolases, particularly those that exhibit indole-3-acetamide amidohydrolase activity. AtAMI1 polypeptide and mRNA are present in leaf tissues, as shown by immunoblotting and RT-PCR, respectively. AtAMI1 was expressed from its cDNA in enzymatically active form and exhibits substrate specificity for indole-3-acetamide, but also some activity against l-asparagine. The recombinant enzyme was characterized further. The results show that higher plants have acylamidohydrolases with properties similar to the enzymes of certain plant-associated bacteria such as Agrobacterium-, Pseudomonas- and Rhodococcus-species, in which these enzymes serve to synthesize the plant growth hormone, indole-3-acetic acid, utilized by the bacteria to colonize their host plants. As indole-3-acetamide is a native metabolite in Arabidopsis thaliana, it can no longer be ruled out that one pathway for the biosynthesis of indole-3-acetic acid involves indole-3-acetamide-hydrolysis by AtAMI1

A Molecular Phylogenomic Analysis of the ILR1-Like Family of IAA Amidohydrolase Genes

The ILR1-like family of hydrolase genes was initially isolated in Arabidopsis thaliana and is thought to help regulate levels of free indole-3-acetic-acid.We have investigated how this family has evolved in dicotyledon, monocotyledon and gymnosperm species by employing the GenBank and TIGR databases to retrieve orthologous genes. The relationships among these sequences were assessed employing phylogenomic analyses to examine molecular evolution and phylogeny. The members of the ILR1-like family analysed were ILL1, ILL2, ILL3, ILL6, ILR1 and IAR3. Present evidence suggests that IAR3 has undergone the least evolution and is most conserved. This conclusion is based on IAR3 having the largest number of total interspecific orthologues, orthologous species and unique orthologues. Although less conserved than IAR3, DNA and protein sequence analyses of ILL1 and ILR1 suggest high conservation. Based on this conservation, IAR3, ILL1 and ILR1 may have had major roles in the physiological evolution of ‘higher’ plants. ILL3 is least conserved, with the fewest orthologous species and orthologues. The monocotyledonous orthologues for most family-members examined have evolved into two separate molecular clades from dicotyledons, indicating active evolutionary change. The monocotyledon clades are: (a) those possessing a putative endoplasmic reticulum localizing signal; and (b) those that are putative cytoplasmic hydrolases. IAR3, ILL1 and ILL6 are all highly orthologous to a gene in the gymnosperm Pinus taeda, indicating an ancient enzymatic activity. No orthologues could be detected in Chlamydomonas, moss and fern databases

Development of a Training Knowledge Management System for the SERVIR Global Network

No abstract availabl