Sustainable Retirement Living: What Matters?

There is a growing demand for sustainable retirement villages in Australia due to an increasing number of ageing population and public acceptance of sustainable development. This research aims to gain a better understanding of retirees’ understanding about sustainable retirement living and their attitudes towards sustainable developments via a questionnaire survey approach. The results showed that the current residents of retirement villages are generally very conscious of unsustainable resource consumption and would like their residences and community to be more environmentally friendly and energy efficient. The cost of energy supply is a concern to majority of respondents. However there is a certain level of concerns from residents too on the extra cost of going green in their residence. Education is required to residents about recycling household waste and how to use available facilities. A better understanding of retirees’ awareness and attitudes towards sustainability issues helps to improve the sustainable developments of retirement villages in the future

Development of Methods for Assessment of the Effects of Sterilisation on Acellular Vascular Grafts

Cardiovascular disease is the highest cause of morbidity worldwide, encompassing blood vessel disorders such as coronary artery and peripheral arterial disease. For small diameter applications (less than 6 mm) a tissue engineered commercial product is yet to be produced. Previously, a process for the production of acellular porcine carotid arteries was developed which showed excellent potential for clinical translation as vascular bypass grafts. Medical products, however, need to be sterilised to a sterility assurance level of 10-6 before they can be used clinically. The aim of this thesis was firstly to produce acellular vascular grafts from porcine carotid arteries using previously developed decellularisation technology. Secondly, to develop robust methods capable of evaluating the effects of different sterilisation methods on the biological and mechanical characteristics of acellular vascular grafts and finally to determine the compatibility of the main stream industrially available sterilisation processes (Gamma and E-Beam irradiation and ethylene oxide treatment) with acellular vascular grafts. Decellularisation of porcine carotid arteries was evaluated by determination of DNA content and histology. Biocompatibility was assessed using contact cytotoxicity. Acellular arteries were then subjected to 30 kGy (25 kGy min) E-Beam or Gamma irradiation or ethylene oxide treatment. The effects of sterilisation were determined using histology, immunohistochemistry, second harmonic generation multiphoton imaging, differential scanning calorimetry, denatured collagen content and determination of mechanical properties compared to non-sterilised acellular arteries. Mechanical properties were assessed using uniaxial tensile testing at a low strain rate to failure and burst pressure and compliance testing Histologically, the architecture of the arteries was retained post decellularisation and DNA content was reduced by greater than 95 %. The arteries were not cytotoxic. Stress strain mechanics were also retained but compliance testing showed a significant reduction. Post sterilisation with both Gamma irradiation and E-beam irradiation, there was a significantly increased stiffness of the elastin and collagen modulus of the acellular arteries. Ethylene oxide treatment significantly increased the elastin and collagen modulus. E-Beam irradiation and ethylene oxide treatment reduced compliance excessively. Histology showed the architecture of gamma and E-beam sterilised arteries to be consistent with acellular arteries and that ethylene oxide treated arteries exhibited some layer separation. Multiphoton imaging showed damage to all sterilised samples with Gamma sterilised acellular arteries the least affected. There were no significant differences in denaturation temperature. These results were in partial agreement with previously reported data on the effects of Gamma and E-beam sterilisation on tendons. Gamma, E-beam [30 kGy] irradiation and ethylene oxide treatment caused detrimental effects to the mechanical properties of acellular porcine carotid arteries. E-beam irradiation and ethylene oxide treatment caused severe reduction in the compliance of acellular porcine carotid arteries. Compliance mismatch is a known failure mechanism of vascular grafts, therefore E-Beam irradiation and ethylene oxide treatment were not compatible with acellular vascular grafts. Gamma irradiation caused the least damaging effects and is the most likely candidate of the three that could potentially be optimised for sterilising acellular vascular grafts

A Collaborative Research Manifesto! An Early Career Response to Uncertainties

Social researchers have been adapting methods and practices in response to COVID-19. In the wake of these adaptations, but still in the midst of intersecting crises that the pandemic has exacerbated or shifted (e.g. health-social-political-economic), researchers face a future suffused with methodological uncertainties. This paper presents a Collaborative Research Manifesto that responds to this by promoting markers for mean-ingful collaborations in future research. The manifesto was co-written primarily through a series of workshops and events that were designed to identify challenges within, and potential for, collaborative research. Through this exploratory collaborative qualitative process, we highlight what the future of such research could look like and describe methodo-logical commitments that collaborative researchers should embody. The discussion draws on wider methodological literature to articulate the key role that ‘collaborative research’ can offer in uncertain times whilst being sensitive of the limitations of our assertive and radical programme

Systems-Scale Analysis Reveals Pathways Involved in Cellular Response to Methamphetamine

Background: Methamphetamine (METH), an abused illicit drug, disrupts many cellular processes, including energy metabolism, spermatogenesis, and maintenance of oxidative status. However, many components of the molecular underpinnings of METH toxicity have yet to be established. Network analyses of integrated proteomic, transcriptomic and metabolomic data are particularly well suited for identifying cellular responses to toxins, such as METH, which might otherwise be obscured by the numerous and dynamic changes that are induced. Methodology/Results: We used network analyses of proteomic and transcriptomic data to evaluate pathways in Drosophila melanogaster that are affected by acute METH toxicity. METH exposure caused changes in the expression of genes involved with energy metabolism, suggesting a Warburg-like effect (aerobic glycolysis), which is normally associated with cancerous cells. Therefore, we tested the hypothesis that carbohydrate metabolism plays an important role in METH toxicity. In agreement with our hypothesis, we observed that increased dietary sugars partially alleviated the toxic effects of METH. Our systems analysis also showed that METH impacted genes and proteins known to be associated with muscular homeostasis/ contraction, maintenance of oxidative status, oxidative phosphorylation, spermatogenesis, iron and calcium homeostasis. Our results also provide numerous candidate genes for the METH-induced dysfunction of spermatogenesis, which have not been previously characterized at the molecular level. Conclusion: Our results support our overall hypothesis that METH causes a toxic syndrome that is characterized by the altered carbohydrate metabolism, dysregulation of calcium and iron homeostasis, increased oxidative stress, and disruption of mitochondrial functions

Genome-wide meta-analysis implicates mediators of hair follicle development and morphogenesis in risk for severe acne

Acne vulgaris is a highly heritable common, chronic inflammatory disease of the skin for which five genetic risk loci have so far been identified. Here, we perform a genome-wide association study of 3823 cases and 16,144 controls followed by meta-analysis with summary statistics from a previous study, with a total sample size of 26,722. We identify 20 independent association signals at 15 risk loci, 12 of which have not been previously implicated in the disease. Likely causal variants disrupt the coding region of WNT10A and a P63 transcription factor binding site in SEMA4B. Risk alleles at the 1q25 locus are associated with increased expression of LAMC2, in which biallelic loss-of-function mutations cause the blistering skin disease epidermolysis bullosa. These findings indicate that variation affecting the structure and maintenance of the skin, in particular the pilosebaceous unit, is a critical aspect of the genetic predisposition to severe acne

Metabolic Profiling of an Echinostoma caproni Infection in the Mouse for Biomarker Discovery

Consumption of raw fish and other freshwater products can lead to unpleasant worm infections. Indeed, such worm infections are of growing public health and veterinary concern, but they are often neglected, partially explained by the difficulty of accurate diagnosis. In the present study we infected 12 mice with an intestinal worm (i.e., Echinostoma caproni) and collected blood, stool, and urine samples 7 times between 1 and 33 days after the infection. At the same time points, blood, stool, and urine were also sampled from 12 uninfected mice. These biofluid samples were examined with a spectrometer and data were analyzed with a multivariate approach. We observed important differences between the infected and the uninfected control animals. For example, we found an increased level of branched chain amino acids in the stool of infected mice and subsequent depletion in blood plasma. Additionally, we observed changes related to a disturbed intestinal bacterial composition, particularly in urine and stool. The combination of results from the three types of biofluids gave the most comprehensive characterization of an E. caproni infection in the mouse. Urine would be the biofluid of choice for diagnosis of an infection because the ease of sample collection and the high number and extent of changed metabolites

Birmingham Environment for Academic Research : Case studies volume 3

This collection of case studies was brought together to showcase the extent and diversity of research that is supported by the University of Birmingham’s Environment for Academic Research (BEAR). BEAR is a collection of contemporary IT resources designed to help research. The following case studies demonstrate how BEAR services such as the Research Data Store (RDS), BEAR software and the University supercomputer BlueBEAR are integral to the progression of important research across disciplines. BlueBEAR is a key component of BEAR, providing compute power and specialist applications free to enable staff and students to delve deeper into their research. Upgraded in 2023, the cluster includes many large memory nodes and a GPU service alongside standard compute nodes. Alongside BlueBEAR, the RDS is a popular choice amongst researchers to securely store their working research data. As of publication, more than 5000 researchers across all five colleges were actively using BlueBEAR and/or the RDS. In this volume, we showcase case studies representing diverse research from every college. From estimating snow coverage to modelling second language acquisition, we show how BEAR services are enabling exciting and important research across the university

The James Webb Space Telescope Mission

Twenty-six years ago a small committee report, building on earlier studies, expounded a compelling and poetic vision for the future of astronomy, calling for an infrared-optimized space telescope with an aperture of at least $4m$. With the support of their governments in the US, Europe, and Canada, 20,000 people realized that vision as the $6.5m$ James Webb Space Telescope. A generation of astronomers will celebrate their accomplishments for the life of the mission, potentially as long as 20 years, and beyond. This report and the scientific discoveries that follow are extended thank-you notes to the 20,000 team members. The telescope is working perfectly, with much better image quality than expected. In this and accompanying papers, we give a brief history, describe the observatory, outline its objectives and current observing program, and discuss the inventions and people who made it possible. We cite detailed reports on the design and the measured performance on orbit.Comment: Accepted by PASP for the special issue on The James Webb Space Telescope Overview, 29 pages, 4 figure

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead