Microfluidic devices for quasi-phase-matching in high-order harmonic generation

The development of compact and bright XUV and soft X-ray sources based on high-order harmonic generation is boosting advances towards understanding the behavior of matter with extreme temporal and spatial resolutions. Here, we report efficient XUV generation inside microfluidic devices fabricated by femtosecond laser irradiation followed by chemical etching. Our microfluidic approach allows one to control and manipulate the generation conditions in gas on a micro-meter scale with unprecedented flexibility, thus enabling a high photon-flux and broadband harmonics spectra up to 200 eV

Assessing the distribution of exotic egg parasitoids of Halyomorpha halys in Europe with a large-scale monitoring program

The brown marmorated stink bug Halyomorpha halys is an invasive agricultural pest with a worldwide distribution. Classical biological control has been identified as the most promising method to reduce the populations of H. halys. Adventive populations of two candidates for releases, Trissolcus japonicus and Trissolcus mitsukurii, have recently been detected in Europe. To assess their distribution and abundance, a large-scale survey was performed. From May to September 2019, a wide area covering northern Italy and parts of Switzerland was surveyed, highlighting the expanding distribution of both Tr. japonicus and Tr. mitsukurii. Within four years after their first detection in Europe, both species have rapidly spread into all types of habitats where H. halys is present, showing a wide distribution and continuous expansion. Both exotic Trissolcus showed high levels of parasitism rate towards H. halys, while parasitization of non-target species was a rare event. The generalist Anastatus bifasciatus was the predominant native parasitoid of H. halys, while the emergence of native scelionids from H. halys eggs was rarely observed. The presence of the hyperparasitoid Acroclisoides sinicus was also recorded. This study provided fundamental data that supported the development of the first inoculative release program of Tr. japonicus in Europe

SHANK3 controls maturation of social reward circuits in the VTA.

Haploinsufficiency of SHANK3, encoding the synapse scaffolding protein SHANK3, leads to a highly penetrant form of autism spectrum disorder. How SHANK3 insufficiency affects specific neural circuits and how this is related to specific symptoms remains elusive. Here we used shRNA to model Shank3 insufficiency in the ventral tegmental area of mice. We identified dopamine (DA) and GABA cell-type-specific changes in excitatory synapse transmission that converge to reduce DA neuron activity and generate behavioral deficits, including impaired social preference. Administration of a positive allosteric modulator of the type 1 metabotropic glutamate receptors mGluR1 during the first postnatal week restored DA neuron excitatory synapse transmission and partially rescued the social preference defects, while optogenetic DA neuron stimulation was sufficient to enhance social preference. Collectively, these data reveal the contribution of impaired ventral tegmental area function to social behaviors and identify mGluR1 modulation during postnatal development as a potential treatment strategy

Aggiornamento sul programma nazionale italiano di lotta biologica classica al moscerino asiatico dei piccoli frutti, Drosophila suzukii

Vengono presentate le attività del Tavolo tecnico-scientifico nazionale di coordinamento per la lotta al moscerino asiatico dei piccoli frutti, Drosophila suzukii Matsumura, istituito formalmente su indicazione del Comitato Fitosanitario Nazionale nel gennaio 2021. La principale finalità del Tavolo è quella di definire e condividere le attività di contrasto al fitofago invasivo attraverso la programmazione di un piano triennale di controllo biologico classico che prevede l’impiego del parassitoide esotico Ganaspis brasiliensis Ihering. La prima campagna di lanci del parassitoide, partita nell’agosto del 2021 a seguito dell’autorizzazione da parte dell’ex Ministero della Transizione Ecologica (ora Ministero dell’Ambiente e della Sicurezza Energetica), ha visto la partecipazione delle regioni Campania, Emilia-Romagna, Piemonte, Puglia, Sicilia, Valle d’Aosta e Veneto e delle Province autonome di Trento e Bolzano. Nel 2023 si aggiungeranno le regioni Lombardia e Toscana. I risultati dei monitoraggi delle prime due stagioni, eseguiti sul 100% dei siti di lancio tra maggio e novembre di ogni anno, suggeriscono che G. brasiliensissi stia insediando in varie aree del territorio nazionale e ne confermano la specificità nei confronti di D. suzukii, già osservata negli studi di laboratorio

A self-consistent method for the simulation of meteor trails with an application to radio observations

Context. Radio-based techniques allow for a meteor detection of 24 h. Electromagnetic waves are scattered by the electrons produced by the ablated species colliding with the incoming air. As the electrons dissipate in the trail, the received signal decays. The interpretation of these measurements entails complex physical modelling of the flow. Aims. In this work, we present a procedure to compute extensive meteor trails in the rarefied segment of the trajectory. This procedure is a general and standalone methodology, which provides meteor physical parameters at given trajectory conditions, without the need to rely on phenomenological lumped models. Methods. We started from fully kinetic simulations of the evaporated gas that describe the nonequilibrium in the flow and the ionisation collisions experienced by metals in their encounter with air molecules. These simulations were employed as initial conditions for performing detailed chemical and multicomponent diffusion calculations of the extended trail, in order to study the processes which lead to the extinction of the plasma. In particular, we focused on the evolution of the trail generated by a 1 mm meteoroid flying at 32 km s−1, above 80 km. We retrieved the ambipolar diffusion coefficient and the electron line density and compared the outcome of our computations with classical results and observational fittings. Finally, the electron field was employed to estimate the resulting reflected signal, using classical radio-echo theory for underdense meteors. Results. A global and constant diffusion coefficient is sufficient to reproduce numerical profiles. A good agreement is found when we compare the extracted diffusion coefficients with theory and observations

The non-psychoactive component of marijuana cannabidiol modulates chemotaxis and IL-10 and IL-12 production of murine macrophages both in vivo and in vitro

Cannabidiol is the main nonpsychoactive component of marijuana. We examined the ability of in vivo and in vitro cannabidiol to interfere with the production of interleukin (IL)-12 and IL-10 by murine macrophages and to modulate macrophage chemotaxis. Cannabidiol added in vitro to peritoneal macrophages significantly increased IL-12 and decreased IL-10 production. The CB1 and CB2 receptor antagonists prevented this modulation. Macrophages from animals treated with cannabidiol at the dose of 30 mg kg(-1) either orally or i.p. produced higher levels of IL-12 and lower levels of IL-10 in comparison to controls, and the CB receptor antagonists did not prevent these effects. Cannabidiol dose-dependently decreased fMLP-induced chemotaxis of macrophages, and the CB2 receptor antagonist prevented this decrease