GUARDA COMPARTILHADA COMO ELEMENTO INIBIDOR DA ALIENAÇÃO PARENTAL

This legal scientific article has as its main purpose to deal with the different types of custody, inserted in the scope of parental alienation. In the same vein, distinguishes parental alienation from Parental Alienation Syndrome (PAS). On the other hand, as an element of containment or even a solution to the problems presented, the institute of shared custody is inserted, where parents have equal responsibility for the life of the minor, not being exempt from the power of the family, being more involved in it, they can participate in the development and personality of the child, receiving respect and admiration from the infant.Este artículo científico jurídico tiene como objetivo principal tratar los diferentes tipos de custodia, insertos en el ámbito de la alienación parental. En la misma línea, distingue la alienación parental del Síndrome de Alienación Parental (PAS). Por otra parte, como elemento de contención o incluso de solución a los problemas presentados, se inserta el instituto de la custodia compartida, donde los padres tienen igual responsabilidad sobre la vida del menor, no estando exentos de la potestad de la familia, siendo más involucrados en ella, pueden participar en el desarrollo y personalidad del niño, recibiendo respeto y admiración por parte del infante.O presente artigo científico jurídico possui como principal finalidade tratar sobre os diferentes tipos de guarda, inseridos no âmbito da alienação parental. Nesse mesmo viés, distingue a alienação parental da Síndrome da Alienação Parental (SAP). Em contrapartida, como um elemento de contenção ou até mesmo solução para os problemas apresentados, insere-se o instituto da guarda compartilhada, onde os pais possuem igual responsabilidade pela vida do menor, não estando isentos do poder da família, estando mais envolvidos nela, podem participar do desenvolvimento e da personalidade da criança, recebendo respeito e admiração do infante.O presente artigo científico jurídico possui como principal finalidade tratar sobre os diferentes tipos de guarda, inseridos no âmbito da alienação parental. Nesse mesmo viés, distingue a alienação parental da Síndrome da Alienação Parental (SAP). Em contrapartida, como um elemento de contenção ou até mesmo solução para os problemas apresentados, insere-se o instituto da guarda compartilhada, onde os pais possuem igual responsabilidade pela vida do menor, não estando isentos do poder da família, estando mais envolvidos nela, podem participar do desenvolvimento e da personalidade da criança, recebendo respeito e admiração do infante

Pulpectomies with Iodoform Versus Calcium Hydroxide-Based Paste: A Preliminary Randomised Controlled Clinical Trial

Objective: To compare clinical and radiographical pulpectomy outcomes in primary teeth filled with different pastes. Material and Methods: The sample included thirty-eight teeth indicated for pulpectomy due to irreversible pulp inflammation or necrosis from thirty patients (2 to 9 years old). The first appointment comprised chemomechanical preparation (2.5% sodium hypochlorite), smear layer removal (6% citric acid), intracanal dressing and temporary restoration. Seven days later, teeth were randomly assigned to filling with iodoform (IP) or calcium hydroxide with zinc oxide (CHZO) based pastes and temporarily restored. Final restoration (composite resin) occurred at the 3rd appointment. Data from baseline, 6 and 12 months were analysed using descriptive and inferential statistics (p≤0.05). Results: The overall frequency of success was 63.6% (n=21), with no significant difference between groups (IP=62.5% n=10; CHZO=64.7% n=11, p=0.59). Multiradicular teeth, overfilled canals and teeth whose coronal restoration have been lost were significantly associated with failure (p=0.01, p=0.04 and p<0.001, respectively). Conclusion: After 12 months, both pastes showed similar outcomes and can be used as good options for pulpectomies in primary teeth. Moreover, tooth location, extent of the root canal filling, and integrity of final restoration during the follow-up influenced the outcome of pulpectomies

Pulpectomies with Iodoform Versus Calcium Hydroxide-Based Paste: A Preliminary Randomised Controlled Clinical Trial

Objective: To compare clinical and radiographical pulpectomy outcomes in primary teeth filled with different pastes. Material and Methods: The sample included thirty-eight teeth indicated for pulpectomy due to irreversible pulp inflammation or necrosis from thirty patients (2 to 9 years old). The first appointment comprised chemomechanical preparation (2.5% sodium hypochlorite), smear layer removal (6% citric acid), intracanal dressing and temporary restoration. Seven days later, teeth were randomly assigned to filling with iodoform (IP) or calcium hydroxide with zinc oxide (CHZO) based pastes and temporarily restored. Final restoration (composite resin) occurred at the 3rd appointment. Data from baseline, 6 and 12 months were analysed using descriptive and inferential statistics (p≤0.05). Results: The overall frequency of success was 63.6% (n=21), with no significant difference between groups (IP=62.5% n=10; CHZO=64.7% n=11, p=0.59). Multiradicular teeth, overfilled canals and teeth whose coronal restoration have been lost were significantly associated with failure (p=0.01, p=0.04 and p<0.001, respectively). Conclusion: After 12 months, both pastes showed similar outcomes and can be used as good options for pulpectomies in primary teeth. Moreover, tooth location, extent of the root canal filling, and integrity of final restoration during the follow-up influenced the outcome of pulpectomies

Can composite packaging and selective enamel etching affect the clinical behavior of bulk-fill composite resin in posterior restorations? 24-month results of a randomized clinical trial

Objectives: This is a double-blind, split-mouth, randomized clinical study that aims to evaluate the influence of bulk-fill composite packaging presented in syringes (BSy) and capsules (BCa), and the effect of selective enamel etching (SEE) on the clinical performance of class I and II bulk-fill resin composite restorations after 24 months. Methodology: A total of 295 class I or class II restorations were performed on 70 patients. One universal adhesive was applied in all restorations. SEE was used in 148 restorations and self-etching mode (SET) in 147 restorations. After the adhesive application, cavities were restored with Filtek Bulk-fill Posterior Restorative in syringes (BSy), Filtek One Bulk-fill in capsules (BCa), or Filtek Supreme Ultra in syringes with the incremental technique (In). All restorations were evaluated using the FDI criteria after one week and after six, 12, and 24 months. Kaplan-Meier survival analysis and Pearson’s Chi-square test were used (α=0.05) for statistical analysis. Results: After 24 months, 62 patients were evaluated and four restorations were lost due to fracture (one for SEEBSy, two for SEEIn, and one for SETIn). No significant differences in the fracture and retention rate were found between groups (p&gt;0.05). SEE showed significantly fewer marginal adaptation defects than SET (p&lt;0.05). BCa and BSy groups showed fewer marginal discrepancies compared to In (p&lt;0.05). Restorations performed with BCa showed less color mismatch than BSy or In (p&lt;0.05). Conclusion: Although all restorations exhibited satisfactory clinical performance after 24 months of clinical service, the clinical behavior of class I and II restorations’ improved when performed with a bulk-fill composite in capsules, mainly when associated with a universal adhesive applied with SEE

Combination of leukocyte and platelet–rich fibrin and demineralized bovine bone graft enhanced bone formation and healing after maxillary sinus augmentation: a randomized clinical trial

Background and objective: The resorption of alveolar ridge bone and maxillary sinus pneumatization are challenges to implant-supported prosthetic rehabilitation. Bone regeneration using bone substitutes and growth factors are alternatives for maxillary sinus augmentation (MSA). Therefore, we sought to evaluate the effects of the association between leukocyte and platelet–rich fibrin (L-PRF) and deproteinized bovine bone mineral (DBBM) in MSA procedures. Materials and methods: Thirty-six maxillary sinuses from 24 individuals were included in this randomized clinical trial. The maxillary sinuses were randomly grafted with LPRF and DBBM (test group) or grafted only with DBBM (positive control). Dental implants were installed in the test group following two periods of evaluation: after 4 (DBBM+LPRF4) and 8 (DBBM+LPFR8) months of sinus graft healing, while the control group received implants only after 8 months. Cone beam computed tomography (CBCT) was taken 1 week after surgery (T1) and before implant placement (T2). Bone samples were collected during implant placement for histomorphometric and immunohistochemical (IHC) analysis. The primary implant stability was assessed by resonance frequency analysis. Results: CBCT analysis demonstrated a significant decrease in bone volume from T1 to T2 in all groups without differences among them. Histologically, the test group showed significantly increase in bone neoformation in both periods of evaluation (LPRF+DBBM4: 44.70±14.01%; LPRF+DBBM8: 46.56±12.25%) compared to the control group (32.34±9.49%). The control group showed the highest percentage of residual graft. IHC analysis showed increased staining intensity of osteocalcin (OCN), vascular endothelial growth factor (VEGF), and runt related transcription factor 2 (RUNX-2) in LPRF+DBBM4 group, and osteopontin (OPN) in the L-PRF+DBBM8. Primary implant stability was successfully achieved (above 60 in implant stability quotient) in all the evaluated groups. Conclusion: Combination of L-PRF and DBBM increased and accelerated new bone formation allowing early implant placement probably due to the higher protein expression of RUNX2, VEGF, OCN, and OPN. These data suggest that the use of L-PRF might be an interesting alternative to use in combination with DBBM for augment the maxillary sinuses allowing the installation of appropriate length implants in shorter period of time. Clinical relevance: This study showed improvement in bone neoformation and accelerated healing when associating L-PRF and DBBM for maxillary sinus augmentation procedures. Trial registration: This study was registered before participant recruitment in Brazilian Registry of Clinical Trials (ReBEC - RBR-95m73t).</p

Panorama atual do modelo de indução da hepatotoxicidade por paracetamol para estudos de hepatoproteção em ratos: Scoping review / Current overview of the model of induction of hepatotoxicity by paracetamol for studies of hepatoprotection in rats: Scoping review

Os medicamentos são considerados xenobióticos pelo organismo humano, isto é, moléculas estranhas. O fígado é o grande responsável pelo processo de metabolização, onde os compostos lipossolúveis são transformados em moléculas com características hidrossolúveis, por meio de reações químicas, com a finalidade de facilitar a excreção renal, principalmente. O paracetamol é um anti-inflamatório da classe não esteroidal (AINES), utilizado para tratamento de dor e febre e pode ser adquirido sem nenhuma prescrição médica, portanto e extremamente utilizado pela população. Mesmo sendo um medicamento de venda livre, este fármaco apresenta um potencial hepatotóxico, dependente de dose, podendo gerar lesão em hepatócitos. Assim, o modelo de lesão hepática induzida por paracetamol é um dos modelos mais populares para testar potenciais agentes hepatoprotetores. No entanto, existem muitas variações neste modelo e a falta de padronização impacta diretamente no tempo de experimento, quantidade de animais utilizados e elevados custos com insumos e fármacos. A presente revisão teve como objetivo reunir informações sobre a padronização do modelo de hepatotoxicidade induzidos por paracetamol, assim como os testes confirmatórios utilizados para garantir a obtenção de resultados que permitem a reprodutibilidade. Para isso foram consultadas as bases de buscada BVS, Pubmed e Scielo, entre os anos de 2015 a 2020. A pesquisa retornou com 370 artigos que, após a triagem foi reduzida a 114, que permitiram o levantamento de dados de extrema relevância para a padronização do modelo, incluindo as análises necessárias para confirmação do desenvolvimento da hepatotoxicidade

FORMAÇÃO DO LICENCIANDO DA UFSCAR PARA ATUAREM COM OS ALUNOS PÚBLICO ALVO DA EDUCAÇÃO ESPECIAL

During teachers formation, it is important their undergraduate course majors have disciplines that address the diversity population they will have in the regular classroom, since they as teachers need a good education, so that their practice will be carried out with quality, with the look of educator directed to the potential of their student. This study sought to understand how does their undergraduate major is carried out at the Federal University of São Carlos, in São Carlos campus, to work with the special education target students. The participants were 67 from different majors offered by UFSCar. Data collection was performed using a semi-structured questionnaire with the participants. The results, demonstrated that there are some gaps in teacher education in Bachelor at UFSCar courses, São Carlos campus and there is a need that must be met in their process of formation concerning to the diversity population. It was conclude that it is necessary to rethink ways to prepare these teachers since the courses they are enrolled do not give any kind of support for a specific formation in a way these teachers be able to prepare activities covering the entire classroom and the special education target students. Keywords: Teacher Training. Educational Inclusion. Special Education. Higher Education. Accessibility.Durante la formación de los docentes, es importante que los cursos de grado disciplinas que abordan la diversidad que los futuros maestros tendrán en el aula regular, como los profesores necesitan una buena educación, por lo que su práctica se lleva a cabo con calidad, con la mirada del educador dirigido al potencial del estudiante. Este estudio trata de comprender cómo la formación de los estudiantes de pregrado de la Universidad Federal de São Carlos, São Carlos campus, para trabajar con el público objetivo de los estudiantes de Educación Especial. Contó con 67 participantes de diferentes grados que ofrece la UFSCar. La recolección de datos se realizó mediante un cuestionario semiestructurada con los participantes. Con los resultados, se observó que existen lagunas en la formación de docentes en los cursos de Licenciatura UFSCar, campus de San Carlos, y es necesario que se deban cumplir en el proceso de formación. Llegamos a la conclusión de que es necesario repensar formas de preparar estos maestros porque los cursos de grado no dan ningún tipo de apoyo a la formación específica para este maestro a preparar las actividades que cubren todas las aulas y que pueda interesar a la audiencia a los estudiantes educación especial. Palabras clave: Formación del profesorado. Inclusión Educativa. Educación Especial. Educación Superior. Acessibilidad.Durante a formação de professores, é importante que haja nos cursos de Licenciatura disciplinas que contemplem a diversidade que os futuros professores terão na sala de aula regular, uma vez que os professores precisam de uma boa formação, para que sua prática seja realizada com qualidade, com o olhar do educador dirigido para a potencialidade do aluno. Esse estudo buscou entender como ocorre a formação dos licenciandos da Universidade Federal de São Carlos, campus de São Carlos, para atuarem com os alunos público alvo da Educação Especial. Contou-se com 67 participantes das diferentes licenciaturas oferecidas pela UFSCar. A coleta de dados foi feita por meio de um questionário semiestruturado com os participantes. Com os resultados, notou-se que há lacunas na formação de professores nos cursos de Licenciatura da UFSCar, campus de São Carlos, e há uma necessidade que precisa ser suprida no seu processo de formação. Conclui-se que se faz necessário repensar formas de preparar esses professores, pois os cursos de licenciatura não dão nenhum tipo de apoio para uma formação específica para que este professor possa preparar atividades que englobem toda sala de aula e que possam abranger os alunos público alvo da educação especial. Palavras-chave: Formação Docente. Inclusão Educacional. Educação Especial. Ensino Superior. Acessibilidade

NOTCH1-mutated chronic lymphocytic leukemia displays high endoplasmic reticulum stress response with druggable potential

IntroductionConstitutive activation of NOTCH1-wild-type (NT1-WT) signaling is associated with poor outcomes in chronic lymphocytic leukemia (CLL), and NOTCH1 mutation (c.7541_7542delCT), which potentiates NOTCH1 signaling, worsens the prognosis. However, the specific mechanisms of NOTCH1 deregulation are still poorly understood. Accumulative evidence mentioned endoplasmic reticulum (ER) stress/unfolded protein response (UPR) as a key targetable pathway in CLL. In this study, we investigated the impact of NOTCH1 deregulation on CLL cell response to ER stress induction, with the aim of identifying new therapeutic opportunities for CLL.MethodsWe performed a bioinformatics analysis of NOTCH1-mutated (NT1-M) and NT1-WT CLL to identify differentially expressed genes (DEGs) using the rank product test. Quantitative real-time polymerase chain reaction (qPCR), Western blotting, cytosolic Ca2+, and annexin V/propidium iodide (PI) assay were used to detect curcumin ER stress induction effects. A median-effect equation was used for drug combination tests. The experimental mouse model Eμ-TCL1 was used to evaluate the impact of ER stress exacerbation by curcumin treatment on the progression of leukemic cells and NOTCH1 signaling.Results and discussionBioinformatics analysis revealed gene enrichment of the components of the ER stress/UPR pathway in NT1-M compared to those in NT1-WT CLL. Ectopic expression of NOTCH1 mutation upregulated the levels of ER stress response markers in the PGA1 CLL cell line. Primary NT1-M CLL was more sensitive to curcumin as documented by a significant perturbation in Ca2+ homeostasis and higher expression of ER stress/UPR markers compared to NT1-WT cells. It was also accompanied by a significantly higher apoptotic response mediated by C/EBP homologous protein (CHOP) expression, caspase 4 cleavage, and downregulation of NOTCH1 signaling in NT1-M CLL cells. Curcumin potentiated the apoptotic effect of venetoclax in NT1-M CLL cells. In Eμ-TCL1 leukemic mice, the administration of curcumin activated ER stress in splenic B cells ex vivo and significantly reduced the percentage of CD19+/CD5+ cells infiltrating the spleen, liver, and bone marrow (BM). These cellular effects were associated with reduced NOTCH1 activity in leukemic cells and resulted in prolonged survival of curcumin-treated mice. Overall, our results indicate that ER stress induction in NT1-M CLL might represent a new therapeutic opportunity for these high-risk CLL patients and improve the therapeutic effect of drugs currently used in CLL