AS03-adjuvanted H7N1 detergent-split virion vaccine is highly immunogenic in unprimed mice and induces cross-reactive antibodies to emerged H7N9 and additional H7 subtypes

AbstractAvian H7 is one of several influenza A virus subtypes that have the potential to cause pandemics. Herein we describe preclinical results following administration of an investigational H7N1 inactivated detergent-split virion vaccine adjuvanted with the AS03 Adjuvant System. The adjuvanted H7N1 vaccine was highly immunogenic compared to the non-adjuvanted H7N1 vaccine in unprimed mice with less than 100ng of hemagglutinin antigen per dose. In addition, compared to the non-adjuvanted vaccine, the AS03-adjuvanted H7N1 vaccine also induced robust HI and VN antibody responses that cross-reacted with other H7 subtypes, including recently emerged H7N9 virus. These H7 data from the preclinical mouse model add to the existing H5 data to suggest that AS03 adjuvant technology may be generally effective for formulating antigen-sparing detergent-split virion vaccines against intrinsically sub-immunogenic avian influenza A virus subtypes

Cross-Protection against Lethal H5N1 Challenge in Ferrets with an Adjuvanted Pandemic Influenza Vaccine

Background. Unprecedented spread between birds and mammals of highly pathogenic avian influenza viruses (HPAI) of the H5N1 subtype has resulted in hundreds of human infections with a high fatality rate. This has highlighted the urgent need for the development of H5N1 vaccines that can be produced rapidly and in sufficient quantities. Potential

A first update on mapping the human genetic architecture of COVID-19

peer reviewe

Development of antigen-loaded microparticles using biodegradable polymers for mucosal vaccination: application to schistosomiasis

Doctorat en sciences biologiques -- UCL, 199

Seasonal activities of female barbel

Two female barbel Rarbus barbus (L.) were tagged with implantable radio transmitters and tracked in the Ourthe River (Southern Belgium) from April 12th to July 7th, 1989 to study their movements and activity rhythms during the spring-summer period. Both barbels defined a 1.6 km home range and occupied four residence areas. Greatest average daily movements within the home range limits occurred during the prespawning and spawning periods and were associated with variations of mean daily water temperature and level. Barbel 2 made a 9.6 km excursion from its spring home range and homed a week later in its residence area. Barbels displayed dusk and dawn activity patterns, character- ized by the occupation of riffle areas. The activity timing was found to be dependent on water temperature while the size of daily activity area was more influenced by river flow conditions

Chapitre 7. Estimation du stock migrateur des anguilles jaunes en grande rivière grâce au contrôle d'échelles à poissons : étude préliminaire dans la Meuse en Belgique

peer reviewedThe migration dynamics of yellow eels in the River Meuse were investigated at the Ampsin navigation weir, using fish passes as tools for discriminating resident and migrating eels. In 1993, 9548 eels (11-65 cm, mean 29,5 cm, pseudo normal distribution) were captured (daily maxima = 818-823 eels) in the two Denil fish passes on the weir. The periodicity of migration was consistant with that in previous years (1989-1993; 44 +/- 7 days, P50 on 1 June). Eels migrated in waves, almost independently of environmental parameters; 3724 eels were marked (Panjet inoculator) and released downstream of the weir. Recapture (2,1 %) suggest an annual rate of migration of 45 km/year sand a migrating flux of about 445.000 fish (15,5 t), with most eels probably migrating through the sluices. Utilization of fish passes to clarify eel stock distribution within the R. Meuse basin is discussed

Single-Dose Mucosal Immunization with Biodegradable Microparticles Containing a Schistosoma mansoni Antigen

The purpose of this work was to assess the immunogenicity of a single nasal or oral administration of recombinant 28-kDa glutathione S-transferase of Schistosoma mansoni (rSm28GST) entrapped by poly(lactide-co-glycolide) (PLG)- or polycaprolactone (PCL)-biodegradable microparticles. Whatever the polymer and the route of administration used, the equivalent of 100 μg of entrapped rSm28GST induced a long-lasting and stable antigen-specific serum antibody response, with a peak at 9 to 10 weeks following immunization. Isotype profiles were comparable, with immunoglobulin G1 being the predominant isotype produced. The abilities of specific antisera to neutralize the rSm28GST enzymatic activity have been used as criteria of immune response quality. Pooled 10-week sera from mice receiving PLG microparticles by the nasal or oral route neutralized the rSm28GST enzymatic activity, whereas sera of mice receiving either PCL microparticles, free rSm28GST, or empty microparticles inefficiently neutralized this enzymatic activity. Finally, this study shows that a single administration of these microparticles could provide distinct and timely release pulses of microencapsulated antigen, which might greatly facilitate future vaccine development