Molecular mapping of rust resistance and genome-wide association study for grain mineral concentration in wheat

This investigation included characterisation of diverse sources for rust resistance and identification of genomic regions underpinning rust resistance in wheat. Markers linked to the adult plant leaf rust resistance gene Lr49 were identified using the 90K SNP (single nucleotide polymorphisms) array genotyping of the VL404/WL711 RIL population and alignment of flow-sorted chromosome sequences of chromosome 4B of parents VL404 and WL711. The Lr49-linked markers were tested on a large VL404/Avocet ‘S’ F2 population for fine mapping of the region. A RIL population of VL404/Avocet ‘S’ was evaluated against stripe rust pathotypes in the greenhouse and the underlying locus was named YrVL. Molecular mapping using the 40K Illumina XT SNP array placed YrVL (769.08-779.3 Mb) on the chromosome arm 2BL and found likely to be a new locus. A stripe rust resistant Tunisian landrace Aus26670 was crossed with the susceptible parent Avocet ‘S’ to develop the Aus26670/AvS RIL population. Seedling tests on this population indicated the presence of a single seedling stripe rust resistance gene and this locus was named YrAW12. Targeted genotyping-by-sequencing assay mapped YrAW12 in the 754.9-763.9 Mb region of chromosome 2BL. QTL (Quantitative trait loci) mapping of adult plant stripe rust response variation suggested the involvement of four QTL for stripe rust resistance in chromosomes 1BL, 5AL, 5BL and 6DS. Two QTL, QYr.sun-5AL (654.5Mb) and QYr.sun-6DS (1.4Mb), appear to be new. Genome-wide association study (GWAS) in a HarvestPlus panel was also undertaken to identify genomic regions conferring rust resistance and 10 minerals. This panel was genotyped using the 90K Infinium SNP array and 13 markers linked with rust resistance genes. GWAS identified six new QTL for rust resistance and 27 known genes/QTL. Forty-one known and 76 new QTL were identified for mineral content. Accessions carrying alien translocations (1B:1R and 2NS) displayed higher accumulation of some minerals

Adoption of smokeless Chulha (Stove) in rural of Meghalaya and its feasibility: A case study

The smokeless chulha is a kind of stove that can directly impact the health of women in rural areas who are burning firewood as cooking fuel. The present study aimed to evaluate the impact of the smokeless chulha and to compare the social and economic benefits associated with it.  The smokeless Chulhas (stoves) were distributed among the villagers in Tuber Kmai village of Meghalaya, India and  its impact was compared with the traditional chulha used by the villagers. The efficacy of the smokeless chulhas was measured and compared with traditional chulhas in term of cooking time, fire wood consumption, gases, volatile compounds and particulate matter emitted. To measure the amount of gases, volatile compounds and particulate matter released, multifunctional air gas and particulate matter Detector (Labart, India) was used. Compared to traditional chulha, smokeless chulha showed 68.7 % reduction in firewood consumption and 45% reduction in cooking time. In addition, smokeless chulha showed 68.9% and 98% reduction in carbon dioxide and carbon monoxide compared to traditional chulha. Further, a significant reduction (p < 0.05) was observed for particulate matter (75%-87%), formaldehyde (75%) and total volatile organic compounds (88.5%). The results indicate that smokeless chulha could save a huge number of natural resources by reducing the cutting of forest. Moreover, smokeless chulha has a positive economic impact on family income.

UTILIZATION OF SAMANYA SHODHANA IN THE PURIFICATION OF EXCESS MERCURY OBTAINED FROM DENTAL OPERATORY- A PRELIMINARY STUDY

Background: Concerns about toxicity of mercury and disposal of excessive mercury has led to decreased usage of mercury in dental profession but still tooth colored restorative materials are not affordable by all the classes of any society. The disposal of excess mercury has always been a matter of concern. Thus, in the present study, we attempted to evaluate a simple procedure from Rasa Shastra using lime powder, garlic and rock salt for recycling of excess mercury obtained from dental operatory.Materials and methods: The excess mercury was recycled by the standard procedure explained in Ayurveda texts (Samanya shodhana) using Sudha churna (lime powder), Lashuna kalka (paste of Allium sativum L.) and Saindhava lavanaa (rock salt). The commercially available mercury and recycled mercury was analyzed by inductively coupled plasma mass spectrometry (ICP-MS) for the detection of elements in ppm level.Results: It was found that the excess impure mercury contained 5138 ppm, 2866.1 ppm and 0.371 ppm of Silver, Copper and Tin respectively. After Shodhana, the level of silver, tin and copper were markedly reduced. Purified mercury showed a level of 119.5ppm silver, 0.5324 ppm copper and 0.3233 ppm tin.Conclusion: Samanya shodhana is a simple promising procedure which can be used for mercury recycling. The procedure doesnot require sophisticated equipments and maneuver. Further, the materials used in the procedure are easily available and affordable at low cost

Molecular mapping of all-stage stripe rust resistance in Indian wheat (Triticum aestivum) cultivar ‘VL404’

Puccinia striiformis f. sp. tritici (Pst), the causal agent of stripe rust of wheat, is a highly evolving fungal pathogen and several widely deployed stripe rust resistance genes have been overcome worldwide often through single step increase in virulence. Development of stripe rust resistant cultivars depends on the availability of widely effective sources of resistance. An Indian wheat cultivar ‘VL404’ exhibited high level of resistance against Australian Pst pathotypes. ‘VL404’ was crossed with a susceptible genotype Avocet ‘S’ (AvS) and an F6 recombinant inbred line (RIL) population was developed. The VL404/AvS RIL population was evaluated at the seedling stage against three Pst pathotypes differing in their virulence profiles. Monogenic segregation for stripe rust response variation was observed in this population and the resistance locus was tentatively named YrVL. Incorporation of stripe rust data into the VL404/AvS genetic map constructed using 40K Wheat-Barley Illumina XT single-nucleotide polymorphism (SNP) array placed YrVL in the long arm of chromosome 2B in the 769.1–779.3 Mb region of the Chinese Spring physical map. YrVL was aligned with the previously reported genes in chromosome 2BL (Yr43, Yr72 and YrAW12) using Pretzel, and it was placed distal to all these genes. Hence, YrVL appears to represent a new resistance locus

Seroprevalence and molecular detection of coxiellosis among cattle and their human contacts in an organized dairy farm

Background: The present investigation of Coxiella burnetii infection in cattle and farm workers on an organized cattle dairy farm, which appears to be the first of its kind in India, was undertaken to assess the status of this largely neglected and masked zoonosis. Methods: A total of 665 samples comprising of serum (n = 224), milk (n = 217) and vaginal swabs (n = 224) collected from milch animals (n = 224) with a history of reproductive disorders were screened. Besides these, ticks (n = 114); animal feed (n = 4) and environmental samples (n = 13) as well as serum (n = 19) of farm workers were also collected. The animal sera and milk samples as well as human sera were tested for antibodies against C. burnetii by commercial ELISA kit, whereas, all the collected samples were subjected to trans-PCR targeting the IS1111 gene of C. burnetii. Results: A high positivity for coxiellosis was detected in sera (29.91%) and milk (26.73%) samples of dairy cattle as well as sera from human contacts (84.21%) by ELISA. The trans-PCR detected the pathogen in 12.94% sera, 14.73% vaginal swabs and 5.53% milk samples of cattle, and in one soil sample, however, the sera of the farm workers and tick were tested negative. Conclusions: The high positivity for coxiellosis among cattle and farm workers highlight the need to undertake extensive epidemiological studies to unravel the trends of C. burnetii infection in India. Keywords: Coxiella burnetii, Coxiellosis, Farm workers, Organized farm, Zoonosi

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Not AvailableThrips palmi (Thysanoptera: Thripidae) is the predominant tospovirus vector in Asia-Pacific region. It transmits economically damaging groundnut bud necrosis virus (GBNV, family Tospoviridae) in a persistent propagative manner. Thrips serve as the alternate host, and virus reservoirs making tospovirus management very challenging. Insecticides and host plant resistance remain ineffective in managing thrips–tospoviruses. Recent genomic approaches have led to understanding the molecular interactions of thrips–tospoviruses and identifying novel genetic targets. However, most of the studies are limited to Frankliniella species and tomato spotted wilt virus (TSWV). Amidst the limited information available on T. palmi–tospovirus relationships, the present study is the first report of the transcriptome-wide response of T. palmi associated with GBNV infection. The differential expression analyses of the triplicate transcriptome of viruliferous vs. nonviruliferous adult T. palmi identified a total of 2,363 (1,383 upregulated and 980 downregulated) significant transcripts. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses showed the abundance of differentially expressed genes (DEGs) involved in innate immune response, endocytosis, cuticle development, and receptor binding and signaling that mediate the virus invasion and multiplication in the vector system. Also, the gene regulatory network (GRN) of most significant DEGs showed the genes like ABC transporter, cytochrome P450, endocuticle structural glycoprotein, gamma-aminobutyric acid (GABA) receptor, heat shock protein 70, larval and pupal cuticle proteins, nephrin, proline-rich protein, sperm-associated antigen, UHRF1-binding protein, serpin, tyrosine–protein kinase receptor, etc., were enriched with higher degrees of interactions. Further, the expression of the candidate genes in response to GBNV infection was validated in reverse transcriptase-quantitative real-time PCR (RT-qPCR). This study leads to an understanding of molecular interactions between T. palmi and GBNV and suggests potential genetic targets for generic pest control.Not Availabl