5,708 research outputs found
Crystallization of the regulatory and effector domains of the key sporulation response regulator Spo0A
The key response-regulator gene of sporulation, spo0A, has been cloned from Bacillus stearothermophilus and the encoded protein purified. The DNA-binding and phospho-acceptor domains of Spo0A have been prepared by tryptic digestion of the intact protein and subsequently crystallized in forms suitable for X-ray crystallographic studies. The DNA-binding domain has been crystallized in two forms, one of which diffracts X-rays to beyond 2.5 Angstrom spacing. The crystals of the phospho-acceptor domain diffract X-rays beyond 2.0 Angstrom spacing using synchrotron radiation
Secret-Sharing for NP
A computational secret-sharing scheme is a method that enables a dealer, that
has a secret, to distribute this secret among a set of parties such that a
"qualified" subset of parties can efficiently reconstruct the secret while any
"unqualified" subset of parties cannot efficiently learn anything about the
secret. The collection of "qualified" subsets is defined by a Boolean function.
It has been a major open problem to understand which (monotone) functions can
be realized by a computational secret-sharing schemes. Yao suggested a method
for secret-sharing for any function that has a polynomial-size monotone circuit
(a class which is strictly smaller than the class of monotone functions in P).
Around 1990 Rudich raised the possibility of obtaining secret-sharing for all
monotone functions in NP: In order to reconstruct the secret a set of parties
must be "qualified" and provide a witness attesting to this fact.
Recently, Garg et al. (STOC 2013) put forward the concept of witness
encryption, where the goal is to encrypt a message relative to a statement "x
in L" for a language L in NP such that anyone holding a witness to the
statement can decrypt the message, however, if x is not in L, then it is
computationally hard to decrypt. Garg et al. showed how to construct several
cryptographic primitives from witness encryption and gave a candidate
construction.
One can show that computational secret-sharing implies witness encryption for
the same language. Our main result is the converse: we give a construction of a
computational secret-sharing scheme for any monotone function in NP assuming
witness encryption for NP and one-way functions. As a consequence we get a
completeness theorem for secret-sharing: computational secret-sharing scheme
for any single monotone NP-complete function implies a computational
secret-sharing scheme for every monotone function in NP
Nonlinear interactions with an ultrahigh flux of broadband entangled photons
We experimentally demonstrate sum-frequency generation (SFG) with entangled
photon-pairs, generating as many as 40,000 SFG photons per second, visible even
to the naked eye. The nonclassical nature of the interaction is exhibited by a
linear intensity-dependence of the nonlinear process. The key element in our
scheme is the generation of an ultrahigh flux of entangled photons while
maintaining their nonclassical properties. This is made possible by generating
the down-converted photons as broadband as possible, orders of magnitude wider
than the pump. This approach is readily applicable for other nonlinear
interactions, and may be applicable for various quantum-measurement tasks.Comment: 4 pages, 2 figures, Accepted to Phys. Rev. Let
Efficient routing of single photons by one atom and a microtoroidal cavity
Single photons from a coherent input are efficiently redirected to a separate
output by way of a fiber-coupled microtoroidal cavity interacting with
individual Cesium atoms. By operating in an overcoupled regime for the
input-output to a tapered fiber, our system functions as a quantum router with
high efficiency for photon sorting. Single photons are reflected and excess
photons transmitted, as confirmed by observations of photon antibunching
(bunching) for the reflected (transmitted) light. Our photon router is robust
against large variations of atomic position and input power, with the observed
photon antibunching persisting for intracavity photon number 0.03 \lesssim n
\lesssim 0.7
New Dependencies of Hierarchies in Polynomial Optimization
We compare four key hierarchies for solving Constrained Polynomial
Optimization Problems (CPOP): Sum of Squares (SOS), Sum of Diagonally Dominant
Polynomials (SDSOS), Sum of Nonnegative Circuits (SONC), and the Sherali Adams
(SA) hierarchies. We prove a collection of dependencies among these hierarchies
both for general CPOPs and for optimization problems on the Boolean hypercube.
Key results include for the general case that the SONC and SOS hierarchy are
polynomially incomparable, while SDSOS is contained in SONC. A direct
consequence is the non-existence of a Putinar-like Positivstellensatz for
SDSOS. On the Boolean hypercube, we show as a main result that Schm\"udgen-like
versions of the hierarchies SDSOS*, SONC*, and SA* are polynomially equivalent.
Moreover, we show that SA* is contained in any Schm\"udgen-like hierarchy that
provides a O(n) degree bound.Comment: 26 pages, 4 figure
A test of the pioneer factor hypothesis using ectopic liver gene activation
The pioneer factor hypothesis (PFH) states that pioneer factors (PFs) are a subclass of transcription factors (TFs) that bind to and open inaccessible sites and then recruit non-pioneer factors (non-PFs) that activate batteries of silent genes. The PFH predicts that ectopic gene activation requires the sequential activity of qualitatively different TFs. We tested the PFH by expressing the endodermal PF FOXA1 and non-PF HNF4A in K562 lymphoblast cells. While co-expression of FOXA1 and HNF4A activated a burst of endoderm-specific gene expression, we found no evidence for a functional distinction between these two TFs. When expressed independently, both TFs bound and opened inaccessible sites, activated endodermal genes, and \u27pioneered\u27 for each other, although FOXA1 required fewer copies of its motif for binding. A subset of targets required both TFs, but the predominant mode of action at these targets did not conform to the sequential activity predicted by the PFH. From these results, we hypothesize an alternative to the PFH where \u27pioneer activity\u27 depends not on categorically different TFs but rather on the affinity of interaction between TF and DNA
Secure self-calibrating quantum random bit generator
Random bit generators (RBGs) are key components of a variety of information
processing applications ranging from simulations to cryptography. In
particular, cryptographic systems require "strong" RBGs that produce
high-entropy bit sequences, but traditional software pseudo-RBGs have very low
entropy content and therefore are relatively weak for cryptography. Hardware
RBGs yield entropy from chaotic or quantum physical systems and therefore are
expected to exhibit high entropy, but in current implementations their exact
entropy content is unknown. Here we report a quantum random bit generator
(QRBG) that harvests entropy by measuring single-photon and entangled
two-photon polarization states. We introduce and implement a quantum
tomographic method to measure a lower bound on the "min-entropy" of the system,
and we employ this value to distill a truly random bit sequence. This approach
is secure: even if an attacker takes control of the source of optical states, a
secure random sequence can be distilled.Comment: 5 pages, 2 figure
Reduction of quantum noise in optical interferometers using squeezed light
We study the photon counting noise in optical interferometers used for
gravitational wave detection. In order to reduce quantum noise a squeezed
vacuum state is injected into the usually unused input port. Here, we
specifically investigate the so called `dark port case', when the beam splitter
is oriented close to 90{\deg} to the incoming laser beam, such that nearly all
photons go to one output port of the interferometer, and only a small fraction
of photons is seen in the other port (`dark port'). For this case it had been
suggested that signal amplification is possible without concurrent noise
amplification [R.Barak and Y.Ben-Aryeh, J.Opt.Soc.Am.B25(361)2008]. We show
that by injection of a squeezed vacuum state into the second input port,
counting noise is reduced for large values of the squeezing factor, however the
signal is not amplified. Signal strength only depends on the intensity of the
laser beam.Comment: 8 pages, 1 figur
Bacterially mediated removal of phosphorus and cycling of nitrate and sulfate in the waste stream of a "zero-discharge" recirculating mariculture system
Simultaneous removal of nitrogen and phosphorus by microbial biofilters has been used in a variety of water treatment systems including treatment systems in aquaculture. In this study, phosphorus, nitrate and sulfate cycling in the anaerobic loop of a zero-discharge, recirculating mariculture system was investigated using detailed geochemical measurements in the sludge layer of the digestion basin. High concentrations of nitrate and sulfate, circulating in the overlying water (~15 mM), were removed by microbial respiration in the sludge resulting in a sulfide accumulation of up to 3 mM. Modelling of the observed S and O isotopic ratios in the surface sludge suggested that, with time, major respiration processes shifted from heterotrophic nitrate and sulfate reduction to autotrophic nitrate reduction. The much higher inorganic P content of the sludge relative to the fish feces is attributed to conversion of organic P to authigenic apatite. This conclusion is supported by: (a) X-ray diffraction analyses, which pointed to an accumulation of a calcium phosphate mineral phase that was different from P phases found in the feces, (b) the calculation that the pore waters of the sludge were highly oversaturated with respect to hydroxyapatite (saturation index = 4.87) and (c) there was a decrease in phosphate (and in the Ca/Na molar ratio) in the pore waters simultaneous with an increase in ammonia showing there had to be an additional P removal process at the same time as the heterotrophic breakdown of organic matter
A Photon Turnstile Dynamically Regulated by One Atom
Beyond traditional nonlinear optics with large numbers of atoms and photons, qualitatively new phenomena arise in a quantum regime of strong interactions between single atoms and photons. By using a microscopic optical resonator, we achieved such interactions and demonstrated a robust, efficient mechanism for the regulated transport of photons one by one. With critical coupling of the input light, a single atom within the resonator dynamically controls the cavity output conditioned on the photon number at the input, thereby functioning as a photon turnstile. We verified the transformation from a Poissonian to a sub-Poissonian photon stream by photon counting measurements of the input and output fields. The results have applications in quantum information science, including for controlled interactions of single light quanta and for scalable quantum processing on atom chips
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