Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

The Applicability of the Drought Index and Analysis of Spatiotemporal Evolution Mechanisms of Drought in the Poyang Lake Basin

Poyang Lake, the largest freshwater lake in China, is an important regional water resource and a landmark ecosystem. In recent years, it has experienced a period of prolonged drought. Using appropriate drought indices to describe the drought characteristics of the Poyang Lake Basin (PLB) is of great practical significance in the face of severe drought situations. This article explores the applicability of four drought indices (including the precipitation anomaly index (PJP), standardized precipitation index (SPI), China Z-index (CPZI), and standardized precipitation evapotranspiration index (SPEI)) based on historical facts. A systematic study was conducted on the spatiotemporal evolution patterns of meteorological drought in the PLB based on the optimal drought index. The results show that SPI is more suitable for the description of drought characteristics in the PLB. Meteorological droughts occur frequently in the summer and autumn in the PLB, with the frequency of mild drought being 17.29% and 16.88%, respectively. The impact range of severe drought or worse reached 22.19% and 28.33% of the entire basin, respectively. The probability of drought occurrence in the PLB shows an increasing trend in spring, while in most areas, it shows a decreasing trend in other seasons, with only a slight increase in the upper reaches of the Ganjiang River (UGR). One of the important factors influencing drought in the PLB is atmospheric circulation. The abnormal variation of the Western Pacific Subtropical High was one of the key factors contributing to the severe drought in the PLB in 2022. This study is based on a long-term series of meteorological data and selects the drought index for the PLB. It describes the spatiotemporal distribution characteristics and evolution patterns of drought and investigates the developmental path and influencing factors of drought in typical years. This study provides a reliable scientific basis for similar watershed water resource management

Association between Cardiac Autonomic Neuropathy and Coronary Artery Lesions in Patients with Type 2 Diabetes

Objective. Cardiac autonomic neuropathy (CAN) is a common and serious complication of diabetes mellitus with various systemic involvements, such as atherosclerotic cardiovascular disease. We aimed to evaluate the association between CAN and coronary artery lesions in patients with type 2 diabetes. Research Design and Methods. We retrospectively reviewed the medical records of 104 patients with type 2 diabetes and coronary artery disease (CAD). We evaluated heart rate variability (HRV) parameters (SDANN, SDNN, and pNN50) to assess cardiac autonomic function. The severity of coronary lesions was assessed by the Gensini scores and the number of affected vessels. Correlation analyses between HRV parameters and the severity of coronary lesions and clinical parameters were performed. Results. Spearman’s correlation analysis showed a significant negative correlation between SDANN and Gensini scores (r=−0.22, P=0.03). Interestingly, this finding remained significant after adjusting for clinical covariates (r=−0.23, P=0.03). However, there was no association between HRV parameters and the severity of coronary lesions as assessed by the number of affected vessels. Clinical parameters were not significantly correlated with HRV parameters (all P>0.05). Conclusions. Cardiac autonomic neuropathy might be related to the degree of coronary atheromatous burden in patients with type 2 diabetes. Screening for cardiac autonomic neuropathy might potentially be beneficial in the risk stratification of patients with type 2 diabetes

Characteristics of natural gas accumulation in the Cambrian weathered crust in southwestern Ordos Basin

The Cambrian weathered crust formed in the Early Paleozoic central paleouplift of the Ordos Basin and its periphery has always been a key target for oil and gas exploration, and important breakthroughs in oil and gas exploration have been made there. In order to further accelerate the process of natural gas exploration in this area, this paper based on systematic analysis of source-reservoir assemblage and trap type in the Cambrian weathered crust in southwestern Ordos Basin, and then evaluates its natural gas exploration potentials and favorable exploration zones. The following research results are obtained. First, the Upper Paleozoic coal-measure source rocks in the basin is the main gas source rocks, forming a source-reservoir assemblage of “upper source and lower reservoir” with Cambrian reservoir. Second, the types of the reservoir in the Cambrian weathered crust are mainly dolomite intercrystalline (dissolution) pores and karst fractures and vugs, whose development is controlled by grain shoal microfacies and karstification in the weathered crust stage, respectively. Third, the traps in the Cambrian weathered crust are mainly lithologic-stratigraphic traps, which were formed in the tectonic uplifting setting of the eastern Ordos Basin during the Yanshanian. There are geological conditions of lithologic and stratigraphic barriers in the updip direction, which is beneficial to the short-distance natural gas migration and accumulation. In conclusion, Huanxian-Zhengning area on the east margin of the central paleouplift in southwestern Ordos Basin is a favorable exploration zone of Cambrian weathered crust gas reservoirs, and the Middle Cambrian Zhangxia Formation and Upper Cambrian Sanshanzi Formation are favorable intervals of oil and gas exploration

Three-dimensional ultrasound fusion imaging in precise needle placement for thermal ablation of hepatocellular carcinoma

AbstractPurpose To investigate the value of three-dimensional ultrasound fusion imaging (3DUS FI) technique for guiding needle placement in hepatocellular carcinoma (HCC) thermal ablation.Methods A total of 57 patients with 60 HCCs with 3DUS FI-guided thermal ablation were retrospectively included in the study. 3DUS volume data of liver were acquired preoperatively by freehand scanning with the tumor and predetermined 5 mm ablative margin automatically segmented. Plan of needle placement was made through a predetermined simulated ablation zone to ensure a 5 mm ablative margin with the coverage rate toward tumor and ablative margin. With real-time ultrasound and 3DUS fusion imaging, ablation needles were placed according to the plan. After ablation, the ablative margin was immediately evaluated by contrast-enhanced ultrasound and 3DUS fusion imaging. The rate of adequate ablative margin, complete response (CR), local tumor progression (LTP), disease-free survival (DFS), and overall survival (OS) was evaluated.Results According to postoperative contrast-enhanced CT or MR imaging, the complete response rate was 100% (60/60), and 83% of tumors (30/36) achieved adequate ablative margin (>5 mm) three-dimensionally. During the follow-up period of 6.0-42.6 months, LTP occurred in 5 lesions, with 1- and 2-year LTP rates being 7.0% and 9.4%. The 1- and 2-year DFS rates were 76.1% and 65.6%, and 1- and 2-year OS rates were 98.1% and 94.0%. No major complications or ablation-related deaths were observed in any patients.Conclusions Three-dimensional ultrasound fusion imaging technique may improve the needle placement of thermal ablation for HCC and reduce the rate of LTP

Enhanced secondary pollution offset reduction of primary emissions during COVID-19 lockdown in China.

To control the spread of the 2019 novel coronavirus (COVID-19), China imposed nationwide restrictions on the movement of its population (lockdown) after the Chinese New Year of 2020, leading to large reductions in economic activities and associated emissions. Despite such large decreases in primary pollution, there were nonetheless several periods of heavy haze pollution in eastern China, raising questions about the well-established relationship between human activities and air quality. Here, using comprehensive measurements and modeling, we show that the haze during the COVID lockdown was driven by enhancements of secondary pollution. In particular, large decreases in NOx emissions from transportation increased ozone and nighttime NO3 radical formation, and these increases in atmospheric oxidizing capacity in turn facilitated the formation of secondary particulate matter. Our results, afforded by the tragic natural experiment of the COVID-19 pandemic, indicate that haze mitigation depends upon a coordinated and balanced strategy for controlling multiple pollutants