Heterogeneity of gene expression of the hemagglutinin-esterase (HE) protein of murine coronaviruses.

The hemagglutinin-esterase (HE) membrane glycoprotein is present only in some members of the coronavirus family, including some strains of mouse hepatitis virus (MHV). In the JHM strain of MHV, expression of the HE gene is variable and corresponds to the number of copies of a UCUAA pentanucleotide sequence present at the 3'-end of the leader RNA. This copy number varies among MHV strains, depending on their passage history. The JHM isolates with two copies of UCUAA in their leader RNA showed a high level of HE expression, whereas the JHM isolate with three copies had a low-level expression. In this study, the analysis of HE gene expression was extended to other MHV strains. The synthesis of HE mRNA in these viruses also correlates with the copy number of UCUAA in the leader RNA and the particular intergenic sequence preceding the HE gene. In one MHV strain, MHV-1, no detectable HE mRNA was synthesized, despite the presence of a proper transcription initiation signal. This lack of HE mRNA expression was consistent with a leader RNA containing three UCUAA copies. However, mutations and deletions within the coding region of the MHV-1 HE gene have generated a stretch of sequence which resembled the transcriptional initiation motif, and was shown to initiate the synthesis of a novel smaller mRNA. These findings strengthened the theory that interactions between leader RNA and transcriptional initiation sequences regulate MHV subgenomic mRNA transcription. Sequence analysis revealed that most MHV strains, through extensive mutations, deletions, or insertions, have lost the complete HE open reading frame, thus turning HE into a pseudogene. This high degree of variation is unusual as the other three structural proteins (spike, membrane, and nucleocapsid) are well-maintained. In contrast to bovine coronavirus, which apparently requires HE for viral replication, the HE protein in MHV may be only an accessory protein which is not necessary for viral replication. JHM and MHV-S, however, have preserved the expression of HE protein

Book Review: Reinventing Organizations: A Guide to Creating Organizations Inspired by the Next Stage in Human Consciousness by Frederic Laloux

Reinventing Organizations: A Guide to Creating Organizations Inspired by the Next Stage of Human Consciousness is a terrific book for anyone interesting in learning about democratizing and debureaucratizing organizations while increasing profits and employee satisfaction, engagement, and well-being. Though this sounds unrealistic and perhaps a bit Utopian, author Frederic Laloux’s thorough research and in-depth reportage of various exemplar organizations around the world, demonstrate that it can be done—but not by anybody. Like any evolutionary system, the forefront of enlightened organizational change begins with a few who become the models and create the systems and conditions for others to follow—when they are ready. This fascinating resource will inspire everyone who wants to learn about the successful transformation of what were once stultifying, disempowering, red-tape-riven workplaces that crush the human spirit. In Reinventing Organizations, we finally see there is a way out—toward building truly remarkable achievement oriented organizations that help the human spirit thrive

Servant Leaders as Change Agents

Servant leadership (SL) produces high performance and employee engagement. Non-SL senior leaders lacking business ethics have caused negative outcomes. This study explored the lived experiences of senior leaders’ decision-making in SL organizations. Maslow’s theories, decision theory, spirituality, Cicero’s virtue theory of ethics; SL comprised the theoretical/conceptual foundation. Data collection used purposive sampling of 18 senior leader participants in SL organizations employing Giorgi’s descriptive psychological structures of experiences analysis method. Findings confirmed ethical decision-making in SL organizations. Recommendations included examining CEO ethical leadership and firm performance interrelations and promoting ethical decision-making at all levels. Conclusions supported teamwork as the one structure of experience. Positive social change implications are an ethical capitalism that occurs by building community and SL organizations

A Phenomenological Investigation of Leader Development and Mindfulness Meditation

Regardless of the gap between the demands of the global work environment and the maturity of leaders, minimal research exists on the trend of the practice of mindfulness meditation and the developmental experiences of leaders. Consequently, scholars have little understanding of how an increasing number of leaders experience mindfulness meditation. The purpose of this study was to understand the perceived impact mindfulness meditation had on leader development for 20 manager-leaders who had a regular (at least 3 days a week) mindfulness meditation practice. The primary recruitment strategy included outreach to potential participants affiliated with professionally oriented mindfulness groups on LinkedIn. The main conceptual framework was Day’s conceptualization of leader development. The central research question addressed leaders’ perceptions and experiences of the impact of mindfulness meditation on their development as leaders. A modified Stevick–Colaizzi–Keen data analysis procedure was used in this study. Key results included the identification of 10 core themes and the associated conclusion that leaders who want to contribute solutions to global challenges will have to access more of their potential, which may require engaging in contemplative practices such as mindfulness meditation. The principal recommendation includes the serious consideration of mindfulness meditation by leaders and organizational decision makers of development investments. This study has implications for positive social change, in that a better understanding of how leaders experience mindfulness meditation may provide direction for leaders and organizations about developmental practices that support leadership effectiveness

Persistent Decadal-Scale Rainfall Variability in the Tropical South Pacific Convergence Zone Through the Past Six Centuries

Modern Pacific decadal variability (PDV) has global impacts; hence records of PDV from the pre-instrumental period are needed to better inform models that are used to project future climate variability. We focus here on reconstructing rainfall in the western tropical Pacific (Solomon Islands; similar to 9.5 degrees S, similar to 160 degrees E), a region directly influenced by PDV, using cave deposits (stalagmite). A relationship is developed between delta O-18 variations in the stalagmite and local rainfall amount to produce a 600 yr record of rainfall variability from the South Pacific Convergence Zone (SPCZ). We present evidence for large (similar to 1.5 m), abrupt, and periodic changes in total annual rainfall amount on decadal to multidecadal timescales since 1423 +/- 5 CE (Common Era) in the Solomon Islands. The timing of the decadal changes in rainfall inferred from the 20th-century portion of the stalagmite delta O-18 record coincides with previously identified decadal shifts in PDV-related Pacific ocean-atmosphere behavior (Clement et al., 2011; Deser et al., 2004). The Solomons record of PDV is not associated with variations in external forcings, but rather results from internal climate variability. The 600 yr Solomon Islands stalagmite delta O-18 record indicates that decadal oscillations in rainfall are a persistent characteristic of SPCZ-related climate variability.Taiwan ROC NSCNTU 101-2116-M-002-009, 102-2116-M-002-016, 101R7625Geological Science

\u27They say\u27: medical students\u27 perceptions of General Practice, experiences informing these perceptions, and their impact on career intention-a qualitative study among medical students in England

\ua9 Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.OBJECTIVES: The number of UK graduates choosing General Practice training remains significantly lower than the current numbers required to meet the demands of the service. This work aims to explore medical students\u27 perceptions of General Practice, experiences which lead to the development of these perceptions, and the ultimate impact of these on career intention. DESIGN: This mixed-methods, qualitative study used focus groups, semistructured interviews, longitudinal audio diary data and debrief interviews to explore and capture the experiences and perceptions of students in their first and penultimate years of university. SETTING: Three English medical schools. PARTICIPANTS: Twenty students were recruited to focus groups from first and fourth/fifth year of study. All students in these years of study were invited to attend. Six students were recruited into the longitudinal diary study to further explore their experiences. RESULTS: This work identified that external factors, internal driving force and the \u27they say\u27 phenomenon were all influential on the development of perceptions and ultimately career intention. External factors may be split into human or non-human influences, for example, aspirational/inspirational seniors, family, peers (human), placements and \u27the push\u27 of GP promotion (non-human). Driving force refers to internal factors, to which the student compares their experiences in an ongoing process of reflection, to understand if they feel General Practice is a career they wish to pursue. The \u27they say\u27 phenomenon refers to a passive and pervasive perception, without a known source, whereby usually negative perceptions circulate around the undergraduate community. CONCLUSION: Future strategies to recruit graduates to General Practice need to consider factors at an undergraduate level. Positive placement experiences should be maximised, while avoiding overtly \u27pushing\u27 GP onto students

Autopsy examination in sudden cardiac death: a current perspective on behalf of the Association for European Cardiovascular Pathology.

In sudden cardiac death, an autopsy is an essential step in establishing a diagnosis of inherited cardiac disease and identifying families that require cardiac screening. To evaluate aspects of post-mortem practice in Europe, a questionnaire was designed and circulated to both clinical and forensic pathologists. There was a 48% response rate and information was obtained from 17 countries. The results showed a wide variety in the management of sudden cardiac death, with a general tendency towards a lack of thorough investigation. In up to 40% of cases, autopsies were not performed in subjects less than 50 years who may have died from cardiac disease. Reasons for this were lack of finance and lack of interest from police, legal authorities, and doctors. Only 50% of pathologists seem to follow a standard protocol for autopsy examination, apparently due to lack of expertise and/or training. When autopsies were performed, histology and toxicology were almost always taken, genetic studies were generally available and retention of the heart for specialist study was usually permitted. Our results suggest that although the standard of practice is appropriate in many centres, many more cases should have autopsies, especially in sudden deaths in subjects less than 50 years

Crossing the Suspension Bridge: Navigating the Road from School Suspension to College Success – How Some Students Have Overcome the Negative Implications of School Suspension to Bridge the Road to College

Out-of-school suspensions for middle and high school students can have negative, long-lasting consequences. Researchers have documented that suspensions have a negative impact on academic development, increase likelihood of dropping out of school, and are associated with a stronger likelihood that students will be involved in the legal system. However, there are students who overcome these negative statistics and matriculate to post-secondary education successfully. This study examines the lived experiences and personal attributes in students’ lives that enabled them to overcome a history of suspension to enter and succeed in higher education. Using Bronfenbrenner’s ecological model, the study’s researchers interviewed individuals who have a history of middle and/or high school suspensions and matriculated to higher education. Findings suggest that sense of belonging; family, home,/school support; and strength of relationships helped participants neutralize the impact and mitigate the negative aspects of suspension