The effect of addition high rape cake and phytase on nutritive value of diets for broiler chickens

The aim of this study was to evaluate the effect of high rape cake content and phytase added to phosphorus and calcium deficient diets on the nutritive value for broiler chickens. Two experiments were performed: a growth experiment on four groups of one-day-old broilers Ross 308, 30 birds per group (6 replications x 5 birds) and a digestibility experiment on 60 chickens divided into four groups of 20 birds (4 replications of 5 birds). The digestibility of the starter diets were evaluated on chickens at 7 days of age and of grower diets at 28 days of age. The diets used in the digestibility tests were the same for the growth trial. In the growth experiment four diets were prepared for the due periods: starters (1-21 day) and growers (22-49 day of chicken life). The control diet (SBM) did not contain rape cake, while experimental diets contained 15% (starters) and 20% (growers) rape cake of Lirajet cultivar. The experimental diet denoted RC HP had P and Ca contents equal the control diet (phosphorus about 7.5 g and calcium about 10 g.kg-1), while the diet denoted as RC LP contained less P and Ca (5.8 g and 6.8 g.kg-1 respectively) than the control and RC HP diets. The diet denoted RC LP+ Phy was supplemented with an enzyme preparation containing phytase at a quantity of 875 FYT.kg-1. Application of 15% of rape cake into starter and 20% into grower diets (RC HP) allowed for similar body weights and feed conversion ratio as the control group, whereas reduction of phosphorus and calcium content in the starter diet (RC LP) significantly decreased body weight at day 21. The addition of phytase to the starter diet with low level of phosphorus and calcium showed the tendency to improve body weight in this period. Application of rape cake into starter and grower diets had poor effects on fat digestibility in all groups, whereas supplementation of grower diets with a low level of phytase phosphorus and calcium improved the digestibility of total phosphorus in comparison to the control and RC HP diets. A tendency to improve the performance results for diets with rape cake (RC HP) in comparison to the control diet in the second period of fattening (grower diets) was observed. Application of rape cake into the diets had a significantly beneficial effect on slaughter yield, fleshiness and fatty acid composition of meat, but not so on the heart muscle, whereas phytase did not have an influence on slaughter results. It was concluded that rape cake can be used in broiler diets, but a quantity of 15% in the starter and 20% in the grower diet may have a negative effect on the heart of so fed birds

Identification of genetic variants associated with Huntington's disease progression: a genome-wide association study

Background Huntington's disease is caused by a CAG repeat expansion in the huntingtin gene, HTT. Age at onset has been used as a quantitative phenotype in genetic analysis looking for Huntington's disease modifiers, but is hard to define and not always available. Therefore, we aimed to generate a novel measure of disease progression and to identify genetic markers associated with this progression measure. Methods We generated a progression score on the basis of principal component analysis of prospectively acquired longitudinal changes in motor, cognitive, and imaging measures in the 218 indivduals in the TRACK-HD cohort of Huntington's disease gene mutation carriers (data collected 2008–11). We generated a parallel progression score using data from 1773 previously genotyped participants from the European Huntington's Disease Network REGISTRY study of Huntington's disease mutation carriers (data collected 2003–13). We did a genome-wide association analyses in terms of progression for 216 TRACK-HD participants and 1773 REGISTRY participants, then a meta-analysis of these results was undertaken. Findings Longitudinal motor, cognitive, and imaging scores were correlated with each other in TRACK-HD participants, justifying use of a single, cross-domain measure of disease progression in both studies. The TRACK-HD and REGISTRY progression measures were correlated with each other (r=0·674), and with age at onset (TRACK-HD, r=0·315; REGISTRY, r=0·234). The meta-analysis of progression in TRACK-HD and REGISTRY gave a genome-wide significant signal (p=1·12 × 10−10) on chromosome 5 spanning three genes: MSH3, DHFR, and MTRNR2L2. The genes in this locus were associated with progression in TRACK-HD (MSH3 p=2·94 × 10−8 DHFR p=8·37 × 10−7 MTRNR2L2 p=2·15 × 10−9) and to a lesser extent in REGISTRY (MSH3 p=9·36 × 10−4 DHFR p=8·45 × 10−4 MTRNR2L2 p=1·20 × 10−3). The lead single nucleotide polymorphism (SNP) in TRACK-HD (rs557874766) was genome-wide significant in the meta-analysis (p=1·58 × 10−8), and encodes an aminoacid change (Pro67Ala) in MSH3. In TRACK-HD, each copy of the minor allele at this SNP was associated with a 0·4 units per year (95% CI 0·16–0·66) reduction in the rate of change of the Unified Huntington's Disease Rating Scale (UHDRS) Total Motor Score, and a reduction of 0·12 units per year (95% CI 0·06–0·18) in the rate of change of UHDRS Total Functional Capacity score. These associations remained significant after adjusting for age of onset. Interpretation The multidomain progression measure in TRACK-HD was associated with a functional variant that was genome-wide significant in our meta-analysis. The association in only 216 participants implies that the progression measure is a sensitive reflection of disease burden, that the effect size at this locus is large, or both. Knockout of Msh3 reduces somatic expansion in Huntington's disease mouse models, suggesting this mechanism as an area for future therapeutic investigation

Effect of Xylanase Application to Rapeseed Cake Diet on Digestibility and Deposition of Nutrients and Energy in Young Broiler Chickens

Two experiments were conducted to study the effect of rapeseed cake and xylanase application on the digestibility and deposition of nutrients and energy in young broiler chickens. Experiment 1 was carried out on broiler chickens between the ages of 1 to 21 days, which were divided into three groups. The control diet contained soybean meal, whereas in the experimental diets a part of the soybean meal was replaced by 15% of rape cakes. Furthermore, an exogenous enzyme preparation containing xylanase in the amount of 0.2g kg-1 was added to one of the two rapeseed cake diets. Seven 1-day-old chickens at the beginning of the experiment and 6 chickens on day 21 of the experiment were slaughtered in each group and the chemical composition of their bodies was analyzed. In experiment 2, a digestibility test of the diets from experiment 1 was carried out on 2-week-old broiler chickens. The application of 15 % rapeseed cakes and the addition of the enzyme preparation did not significantly influence body weight, feed intake, the chemical composition or the energy content in the chickens, bodies. Differences in the digestibility of the dry matter , ether extract , and crude phosphorus of the diets were statistically significant. The highest digestibility of dry matter, ether extract, and crude phosphorus(P<0.05) was found in the control group. Application of xylanase into the diet containing rapeseed cake increased the digestibility of nutrients, but the differences obtained between the groups were not significant

Knowledge of Medical Students and Medical Professionals Regarding Nutritional Deficiencies in Patients with Celiac Disease

A gluten-free diet provides relief from symptoms for patients with celiac disease, although there is still a risk of nutritional deficiencies. These patients can potentially consume an excessive amount of fat and insufficient amounts of fiber, iron, vitamin D, and calcium. This study aimed to assess the knowledge of medical students and healthcare professionals in Poland regarding nutritional deficiencies and the prevention of such deficiencies in patients with celiac disease who are on a gluten-free diet. Of the 430 survey participants, 46% did not realize the risk of nutritional deficiencies in patients with celiac disease. The knowledge of the participants was lowest regarding the risk of being overweight or obese. Among the healthcare professionals, an acceptable level of correct answers was provided by only 37% of individuals and was highest for the dietitians’ group. Our results demonstrate the need to improve the education of healthcare professionals concerning nutrition in patients with celiac disease

Żółtakowatość mózgowo-ścięgnista : rzadka przyczyna zespołu rdzeniowo-móżdżkowego

A 34-year-old patient demonstrating pyramidal and cerebellar signs, accompanied by epilepsy, peripheral neuropathy, mental retardation and bilateral cataract was diagnosed with cerebrotendinous xanthomatosis based on the clinical picture, magnetic resonance imaging of the brain and serum sterol analysis. Tendon xanthomas were not observed in this case. After establishing the diagnosis, treatment with chenodeoxycholic acid and statin was introduced. During the next two years of the follow-up, serum cholestanol and 7α-hydroxycholesterol levels decreased in response to the therapy, but this was not reflected in the patient's neurological condition, which was slowly progressing. Treatment effectiveness in cerebrotendinous xanthomatosis is variable, notably better in patients who had started therapy before the injury to the nervous system took place. The present case report points to cerebrotendinous xanthomatosis as a rare cause of spinocerebellar syndrome, which might be treatable if diagnosed in early life

Żółtakowatość mózgowo-ścięgnista: rzadka przyczyna zespołu rdzeniowo-móżdżkowego

A 34-year-old patient demonstrating pyramidal and cerebellar signs, accompanied by epilepsy, peripheral neuropathy, mental retardation and bilateral cataract was diagnosed with cerebrotendinous xanthomatosis based on the clinical picture, magnetic resonance imaging of the brain and serum sterol analysis. Tendon xanthomas were not observed in this case. After establishing the diagnosis, treatment with chenodeoxycholic acid and statin was introduced. During the next two years of the follow-up, serum cholestanol and 7α-hydroxycholesterol levels decreased in response to the therapy, but this was not reflected in the patient's neurological condition, which was slowly progressing. Treatment effectiveness in cerebrotendinous xanthomatosis is variable, notably better in patients who had started therapy before the injury to the nervous system took place. The present case report points to cerebrotendinous xanthomatosis as a rare cause of spinocerebellar syndrome, which might be treatable if diagnosed in early life.U 34-letniego chorego z objawami piramidowymi i móżdżkowymi, padaczką, neuropatią obwodową, upośledzeniem umysłowym oraz obustronną zaćmą na podstawie obrazu klinicznego, badania rezonansu magnetycznego mózgu i oznaczenia stężenia steroli w surowicy rozpoznano żółtakowatość mózgowo-ścięgnistą. Nie obserwowano u tego chorego żółtaków ścięgien. Do leczenia włączono kwas chenodeoksycholowy oraz statynę. Chociaż podczas kolejnych dwóch lat obserwacji stwierdzono zmniejszenie stężenia cholestanolu i 7α-hydroksycholesterolu w surowicy chorego w odpowiedzi na leczenie, jego stan neurologiczny stopniowo się pogarszał. Odpowiedź na leczenie żółtakowatości mózgowo-ścięgnistej jest zróżnicowana; lepsza u chorych, u których leczenie rozpoczęto, zanim wystąpiły zmiany w ośrodkowym układzie nerwowym. Przedstawiany opis przypadku zwraca uwagę na żółtakowatość mózgowo-ścięgnistą jako rzadką przyczynę zespołu rdzeniowo-móżdżkowego, który mógłby być uleczalny, jeśli zostałby rozpoznany na wczesnym etapie życia