Thrombosis with Behçet’s disease should be evaluated different conditions

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Quantitative characteristics of rainbow trout (Oncorhynchus mykiss) semen throughout the reproductive season

The aim of this study was to determine changes in quantitative characteristics of the rainbow trout semen throughout the reproductive season. Semen samples were collected from October to May from broodstock males (n = 12) kept under natural photoperiod regime and semen volume, density, motility, duration of motility, spermatocrit and pH parameters were investigated. In October, one of the 12 brooders (8.3%) were spermiating, increasing to 12 (100%) in December and dropping to 3 (25%) in May. Semen volume significantly increased in January and February. The total volume of expressible semen was maximal in January, increasing from a mean value of 1.7 ml to 20.9 ml. The spermatozoa density showed an increasing trend from October to January, with mean values ranging between 1.4 and 10.3 x 109 sperm/ml. The spermatocrit did not vary with the sampling date. The semen samples collected in November showed the lowest motility of spermatozoa (40%). But the percentage of motile spermatozoa collected from January to May showed more than 80% motility. The duration of motility for the monitoring period ranged between 58.0 and 375.6 sec. In conclusion, the highest quality semen was collected during the medial part of the reproductive season.Bu çalışmanın amacı üreme mevsimi boyunca gökkuşağı alabalığı spermasının nicel özelliklerindeki değişimleri belirlemektir. Sperma örnekleri doğal fotoperiyot altında tutulan damızlıklardan (n = 12) ekim kasım ayları arasında toplandı. Sperma miktarı ve pH, sperma yoğunluğu ve motilite, motil kalma süresi, spermatokrit belirlendi. Ekimde 12 damızlıktan bir tanesi (%8.3) sperm verirken, aralık ayında sperm veren birey sayısı 12’ye (%100) yükseldi ve mayıs ayında 3 (%3) bireye düştü. Sperma miktarı ocak ve şubat aylarında önemli ölçüde arttı. Sağılabilen sperma miktarı, 1.7 ml’den 20.9 ml’ye yükselerek, ocak ayında en yüksek düzeye çıktı. Ekimde 1.4 x 109 sperm/ml olan spermatozoa yoğunluğu ocak ayında 10.3 x 109 sperm/ml olarak saptandı. Spermatokrit değerleri örnekleme zamanına bağlı olarak değişim göstermedi. En düşük motilite kasımdaki örneklerde, ocak ve mayıs arasında toplanan örneklerde ise %80’den yüskek motilite saptandı. Motil kalma süresi, üreme mevsimi boyunca, 58.0 ve 375.6 s arasında değişti. Sonuç olarak, yüksek kalitede sperma üreme mevsimi ortasında toplandı

Tumors of the labial mucosa:a retrospective study of 1045 biopsies

To investigate the relative frequency of localized mucosal swellings of the upper and lower labial mucosa, the clinical-pathological diagnosis agreement and whether patient?s age and gender and tumor?s site and size may raise the suspicion of neoplasm. Retrospective analysis was performed on upper or lower labial mucosal tumors, histopathologically diagnosed between 2009-2018. The diagnostic categories developmental/reactive tumors, benign and malignant neoplasms were associated with patient?s age and gender and tumor?s site and size; clinical-pathological diagnosis agreement was, also, evaluated. Overall, 1000 (95.7%) developmental/reactive tumors, 35 (3.3%) benign and 10 (1%) malignant neoplasms were found. Upper/lower lip tumor ratio was 0.14:1. The diagnostic category was significantly associated with age (p1cm were independent predictors for neoplasms. Patients presenting 2 or 3 of these variables were 20.2 times (p?1cm in patients?60 years have significantly higher probability to be neoplasms

Elevation Effects on Air Temperature in a Topographically Complex Mountain Valley in the Spanish Pyrenees

© 2020 by the authors.Air temperature changes as a function of elevation were analyzed in a valley of the Spanish Pyrenees. We analyzed insolation, topography and meteorological conditions in order to understand how complex topoclimatic environments develop. Clustering techniques were used to define vertical patterns of air temperature covering more than 1000 m of vertical elevation change. Ten locations from the bottom of the valley to the summits were monitored from September 2016 to June 2019. The results show that (i) night-time lapse rates were between −4 and −2 °C km−1, while in the daytime they were from −6 to −4 °C km−1, due to temperature inversions and topography. Daily maximum temperature lapse rates were steeper from March to July, and daily minimum temperatures were weaker from June to August, and in December. (ii) Different insolation exposure within and between the two analyzed slopes strongly influenced diurnal air temperatures, creating deviations from the general lapse rates. (iii) Usually, two cluster patterns were found (i.e., weak and steep), which were associated with stable and unstable weather conditions, respectively, in addition to high-low atmospheric pressure and low-high relative humidity. The results will have direct applications in disciplines that depend on air temperature estimations (e.g., snow studies, water resources and sky tourism, among others).This study was funded by the Spanish Ministry of Economy and Competitiveness project CGL2017-82216-R (HIDROIBERNIEVE). Navarro-Serrano, F. is the recipient of a pre-doctoral FPU grant (Spanish Ministry of Education, Culture and Sports, FPU15/00742). Revuelto, J. is supported by the INDECIS project, which is part of ERA4CS, and by ERA-NET, initiated by JPI Climate, and funded by FORMAS (SE), DLR (DE), BMWFW (AT), IFD (DK), MINECO (SP), ANR (FR) with co-funding by the European Union (Grant 690462). Alonso-González, E. and Aznárez-Balta, M. are beneficiaries of a pre-doctoral FPI grant (Spanish Ministry of Economy and Competitiveness, BES-2015-071466 and PRE2018-084295), and Azorin-Molina, C. of the Ramon y Cajal fellowship (RYC-2017-22830).Peer reviewe

Reducing industrial energy demand in the UK: A review of energy saving technologies and maximum potential in the selected sectors

Currently UK industrial and manufacturing sectors are facing dual challenges of contributing to national 80% reduction targets in CO2 emissions by 2050 (compared to 1990 levels) and improving economic competitiveness in the face of low cost imports. Since energy consumption is the main source of CO2 emissions and directly related to products being manufactured, improving energy efficiency in energy intensive sectors is key to achieve CO2 targets. Energy consumption is unlikely to meet the targets unless energy efficiency opportunities and technologies are fully explored and timely changes are made to business models and policies This study explores potential energy efficiency improvements from three perspectives: system efficiency of steam networks, waste heat recovery technologies and bioenergy/waste utilisation. Two UK energy-intensive sectors, iron and steel, and food and drink, are selected for analysis and discussion. Potential business models for energy efficiency are also reviewed as there are now a variety of energy service companies who can support adoption of appropriate technologies. Furthermore, drivers and barriers to the adoption of energy efficiency technologies are considered in this paper revealing the factors affecting the diffusion of energy efficient and waste heat recovery technologies and their interactions and interdependencies to energy consumptions. Findings show that it is possible to achieve energy consumption reduction in excess of 15% from a technical point of view, however improving energy efficiency in UK industry has been hindered due to some inter-related technical, economic, regulatory and social barriers. The findings help to demonstrate the significant potential for energy efficiency improvement in two industrial sectors, as well as showing the specific types of technologies relevant for different sectoral processes. The range of business models show opportunities for implementation and for developing innovative business models, addressing barriers, and using enablers to accelerate the diffusion of energy efficiency technologies in UK industry

Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9

CRISPR/Cas9 technologies have been employed for genome editing to achieve gene knockouts and knock-ins in somatic cells. Similarly, certain endogenous genes have been tagged with fluorescent proteins. Often, the detection of tagged proteins requires high expression and sophisticated tools such as confocal microscopy and flow cytometry. Therefore, a simple, sensitive and robust transcriptional reporter system driven by endogenous promoter for studies into transcriptional regulation is desirable. We report a CRISPR/Cas9-based methodology for rapidly integrating a firefly luciferase gene in somatic cells under the control of endogenous promoter, using the TGFβ-responsive gene PAI-1. Our strategy employed a polycistronic cassette containing a non-fused GFP protein to ensure the detection of transgene delivery and rapid isolation of positive clones. We demonstrate that firefly luciferase cDNA can be efficiently delivered downstream of the promoter of the TGFβ-responsive gene PAI-1. Using chemical and genetic regulators of TGFβ signalling, we show that it mimics the transcriptional regulation of endogenous PAI-1 expression. Our unique approach has the potential to expedite studies on transcription of any gene in the context of its native chromatin landscape in somatic cells, allowing for robust high-throughput chemical and genetic screens

Long-term precipitation in Southwestern Europe reveals no clear trend attributable to anthropogenic forcing

We present a long-term assessment of precipitation trends in Southwestern Europe (1850-2018) using data from multiple sources, including observations, gridded datasets and global climate model experiments. Contrary to previous investigations based on shorter records, we demonstrate, using new long-term, quality controlled precipitation series, the lack of statistically significant long-term decreasing trends in precipitation for the region. Rather, significant trends were mostly found for shorter periods, highlighting the prevalence of interdecadal and interannual variability at these time-scales. Global climate model outputs from three CMIP experiments are evaluated for periods concurrent with observations. Both the CMIP3 and CMIP5 ensembles show precipitation decline, with only CMIP6 showing agreement with long term trends in observations. However, for both CMIP3 and CMIP5 large interannual and internal variability among ensemble members makes it difficult to identify a trend that is statistically different from observations. Across both observations and models, our results make it difficult to associate any declining trends in precipitation in Southwestern Europe to anthropogenic forcing at this stage

Dietary protected fat and conjugated linoleic acid improves ewe milk fatty acid composition

Publication history: Accepted - 4 february 2023; Published - 15 May 2023.The effects of protected fats (Optima 100) and conjugated linoleic acid (Endulac®-CLA) supplementation on sheep milk saturated and unsaturated fatty acid composition were investigated. Sheep were divided into four experimental groups (15 ewes/group) including: i) a control group - basal diet without any nutritional supplements; ii) experimental group 1 - basal diet + 12g/sheep/day of the protected source of fats in the feed; iii) group 2 - 12 g of CLA in the feed; iv) group 3 - 12 g of protected fats and CLA in feed. Sixty milk fatty acids were different in milk from treated fat and CLA-treated sheep compared to the control group. The most biologically important fatty acid constituents of milk were identified as butyric, caproic, caprylic, lauric, myristic, palmitic, stearic, arachidonic, behenic, oleic, and linoleic acid (C4 to C18). Ewes that received protected fat or CLA, or both, displayed an increased concentration of oleic acid compared to the control. Both treatments modified milk lipid quality parameters and increased the polyunsaturated/saturated fatty acids ratio (PUFA/SFA), the polyunsaturation index (PI), and the thrombogenic index (TI). Group 3 had similar milk lipid quality parameters as untreated animals. Compared to the CLA and control groups, milk production in the protected fat treatment was higher in Turcana dairy ewes. The inclusion of protected fats and CLA as dietary supplements in lactating ewes modified the milk fatty acid profile, with a concomitant impact on suckling lamb performance and consumer health.This work was funded by a research grant awarded to L.S. by Banats University of Agricultural and Veterinary Medicine, King Michel First from Timisoara, Romani