Fast determination of 40 drugs in water using large volume direct injection liquid chromatography–tandem mass spectrometry

This work describes a rapid analytical method based on direct sample injection of water samples for the simultaneous identification/quantification of 40 emerging compounds, including pharmaceuticals and drugs of abuse. The water samples were analyzed by ultra-high-performance liquid chromatography coupled to hybrid triple quadrupole mass spectrometer (UHPLC–MS/MS QqQ). Taking profit of the increasing sensitivity of nowadays tandem mass spectrometers, direct sample injection of large volumes has been an attractive alternative to pre-concentration steps. In this work, the developed methodology has been validated at three concentration levels (10, 100 and 1000 ng/L) in 10 different water samples of different types (5 effluent wastewater and 5 surface water samples). The majority of compounds could be satisfactory validated at these concentrations, showing good recoveries and precision. With only few exceptions, the limits of quantification (LOQs), estimated from the sample chromatogram at lowest spiked level tested, were below 3 ng/L. The method was applied to the analysis of 10 effluent wastewater and 10 surface water samples. Venlafaxine was the compound most frequently detected (80%) in surface water, followed by acetaminophen (70%). Regarding effluent wastewater, valsartan and 4-acetyl aminoantipyrine were detected in 9 out of 10 samples analyzed. These two compounds together with 4-formyl aminoantipyrine and naproxen showed the highest concentrations (>2000 ng/L). In these cases, a dilution step was required for a correct quantification. As an additional evaluation of the method performance, the same water samples were analyzed in another laboratory by a second analytical methodology, based on on-line solid-phase-extraction coupled to LC–MS/MS (QqQ).The authors are very grateful to Serveis Centrals d׳Instrumentació Científica (SCIC) of University Jaume I for using the Xevo TQS mass spectrometer. The financial support of the Centro de Desarrollo Tecnológico Industrial (CDTIIDI-20110823/EXP00044257), of the Spanish Ministry of Education and Science (Ref CTQ2012-36189) and of Generalitat Valenciana (research group of excellence PROMETEO/2009/054; Collaborative Research on Environment and Food Safety, ISIC/2012/016) is acknowledged