1,202 research outputs found
Radio-frequency driven polarization transfer without heteronuclear decoupling in rotating solids
3D NMR spectroscopy for resonance assignment and structure elucidation of proteins under MAS: novel pulse schemes and sensitivity considerations
Moleculare-level secondary structure, polymorphism, and dynamics of full-length alpha-synuclein fibrils studies by solid-state NMR
Π§ΠΈΡΠ»Π΅Π½Π½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π²Π»ΠΈΡΠ½ΠΈΡ ΡΡΠ»ΠΎΠ²ΠΈΠΉ ΡΠ°ΡΠΏΡΠ»Π΅Π½ΠΈΡ Π²ΠΎΠ΄Ρ Π½Π° ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ Π² ΡΠ»Π΅Π΄Π΅ "Π²ΠΎΠ΄ΡΠ½ΠΎΠ³ΠΎ ΡΠ½Π°ΡΡΠ΄Π°"
Π‘ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π½Π΅ΡΠΊΠΎΠ»ΡΠΊΠΈΡ
ΠΌΠΎΠ΄Π΅Π»Π΅ΠΉ "Π²ΠΎΠ΄ΡΠ½ΡΡ
ΡΠ½Π°ΡΡΠ΄ΠΎΠ²" ΠΌΠ°Π»ΡΡ
ΡΠ°Π·ΠΌΠ΅ΡΠΎΠ² Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΎ ΡΠΈΡΠ»Π΅Π½Π½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ°ΠΊΡΠΎΡΠΊΠΎΠΏΠΈΡΠ΅ΡΠΊΠΈΡ
Π·Π°ΠΊΠΎΠ½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΠ΅ΠΉ ΠΈΡΠΏΠ°ΡΠ΅Π½ΠΈΡ ΠΌΠΎΠ½ΠΎΠ΄ΠΈΡΠΏΠ΅ΡΡΠ½ΠΎΠΉ ΡΠΎΠ²ΠΎΠΊΡΠΏΠ½ΠΎΡΡΠΈ ΠΊΠ°ΠΏΠ΅Π»Ρ ΡΠΎΠ½ΠΊΠΎΡΠ°ΡΠΏΡΠ»Π΅Π½Π½ΠΎΠΉ Π²ΠΎΠ΄Ρ ΠΏΡΠΈ ΠΏΡΠΎΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΠΈ ΡΠ΅ΡΠ΅Π· Π²ΡΡΠΎΠΊΠΎΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ½ΡΠ΅ ΠΏΡΠΎΠ΄ΡΠΊΡΡ ΡΠ³ΠΎΡΠ°Π½ΠΈΡ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Ρ Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΈΠ½ΡΠ΅Π³ΡΠ°Π»ΡΠ½ΡΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ ΡΠ΅ΠΏΠ»ΠΎΠΌΠ°ΡΡΠΎΠΏΠ΅ΡΠ΅Π½ΠΎΡΠ° ΠΎΡ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΏΠ»ΠΎΡΠ°Π΄Π΅ΠΉ ΠΈΡΠΏΠ°ΡΠ΅Π½ΠΈΡ ΠΈ ΠΏΠ»ΠΎΡΠ°Π΄Π΅ΠΉ, Π·Π°Π½ΠΈΠΌΠ°Π΅ΠΌΡΡ
"Π²ΠΎΠ΄ΡΠ½ΡΠΌ ΡΠ½Π°ΡΡΠ΄ΠΎΠΌ". ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΡΡΠ»ΠΎΠ²ΠΈΡ ΡΠ°ΡΠΏΡΠ»Π΅Π½ΠΈΡ Π²ΠΎΠ΄Ρ Π΄Π»Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΡ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ Π² Π·ΠΎΠ½Π΅ Π³ΠΎΡΠ΅Π½ΠΈΡ
Probing structure and dynamics in poly[2,2 '-(m-phenylene)-5,5 '-bibenzimidazole] fuel cells with magic-angle spinning NMR
Secondary chemical shifts in immobilized peptides and membrane proteins: A qualitative basis for structure refinement under Magic Angle Spinning.
Solid-state NMR reveals structural differences between fibrils of wild-type and disease-related A53T mutant Ξ±-synuclein.
Π€ΡΠ»ΠΎΡΠΎΡΡΡ ΠΏΡΠ°Π²Π° Π² ΡΠΈΡΡΠ΅ΠΌΡ Π½Π°ΡΠΊ
Thermosensitive amphiphilic block copolymers self-assemble into micelles above their lower critical solution temperature in water, however, the micelles generally display mediocre physical stability. To stabilize such micelles and increase their loading capacity for chemotherapeutic drugs, block copolymers with novel aromatic monomers were synthesized by free radical polymerization of N-(2-benzoyloxypropyl methacrylamide (HPMAm-Bz) or the corresponding naphthoyl analogue (HPMAm-Nt), with N-(2-hydroxypropyl) methacrylamide monolactate, using a polyethylene glycol based macroinitiator. The critical micelle temperatures and critical micelle concentrations decreased with increasing the HPMAm-Bz/Nt content. The micelles of 30-50 nm were prepared by heating the polymer aqueous solutions from 0 to 50 degrees C and were colloidally stable for at least 48 h at pH 7.4 and 37 degrees C. Paclitaxel and docetaxel encapsulation was performed by mixing drug solutions in ethanol with polymer aqueous solutions and heating from 0 to 50 degrees C. The micelles had a drug loading capacity up to 34 wt % for docetaxel, which is among the highest loadings reported for polymeric micelles, with loaded micelle sizes ranging from 60 to 80 nm. The micelles without aromatic groups almost completely released loaded paclitaxel in 10 days, whereas the HPMAm-Bz/Nt containing micelles released 50% of the paclitaxel at the same time, which showed a better retention for the drug of the latter micelles. (1)H solid-state NMR spectroscopy data are compatible with pi-pi stacking between aromatic groups. The empty micelles demonstrated good cytocompatibility, and paclitaxel-loaded micelles showed high cytotoxicity to tumor cells. In conclusion, the pi-pi stacking effect introduced by aromatic groups increases the stability and loading capacity of polymeric micelles
Analysis of microsatellite instability in colorectal carcinoma by microfluidic-based chip electrophoresis
Microsatellite analysis is an important tool in clinical research and molecular diagnostics because microsatellite instability (MSI) occurs frequently in various types of cancer. Approximately 10β15% of colorectal, gastric and endometrial carcinomas are associated with MSI, and this has an impact on clinical prognosis. The microsatellite loci Bat25, Bat26, D2S123, D5S346 and D17S250, recommended by the Bethesda guidelines, were analysed by microfluidic-based on-chip electrophoresis in 40 cases of colon carcinoma with known MSI status. In all cases, microfluidic separation of the PCR amplicons resulted in highly resolved, distinct patterns of each of the five microsatellite loci. Detection of MSI could be demonstrated by microsatellite-loci-associated, well-defined deviations in the electropherogram profiles of tumour and non-tumour material, and confirmed the classification of MSI cases performed by conventional technology. In conclusion, microfluidic chip technology is a simple and reliable approach for MSI detection that allows label-free and very fast analysis of microsatellite amplicons
Solid state NMR sequential resonance assignments and conformational analysis of the 2 x 10.4 kDa dimeric form of the Bacillus subtilis protein Crh
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