<i>Vibrio breoganii</i> sp. nov., a non-motile, alginolytic, marine bacterium within the <i>Vibrio halioticoli</i> clade

Seven non-motile, facultatively anaerobic, alginolytic marine bacteria were isolated from the cultured clams Ruditapes philippinarum and Ruditapes decussatus. Phylogenetic analysis based on 16S rRNA gene sequences showed that these marine bacteria were closely related to the recently described species Vibrio comitans, Vibrio rarus and Vibrio inusitatus (=99.0?% sequence similarity). Phylogenetic analysis based on the housekeeping genes rpoA, recA and atpA grouped the isolates together and allocated them to the Vibrio halioticoli species group. Amplified fragment length polymorphism DNA fingerprinting also grouped them together and enabled them to be differentiated from recognized species of the V. halioticoli clade. DNA–DNA hybridizations showed that the isolates belonged to a novel species; phenotypic features such as the ability to grow at 4 °C and in the presence of 6?% NaCl also enabled them to be separated from other species. The DNA G+C content of RD 15.11T is 44.4 mol%. The genotypic and phenotypic data showed that the isolates represent a novel species in the V. halioticoli clade. The name Vibrio breoganii sp. nov. is proposed, with RD 15.11T (=CECT 7222T =LMG 23858T) as the type strain

Two-dimensional proteome reference map of Vibrio tapetis, the aetiological agent of brown ring disease in clams

Aims: Vibrio tapetis is the etiological agent of brown ring disease (BRD) in clams, one of the most threatening diseases affecting this commercially important bivalve. In this study we have constructed a proteome reference map of the V. tapetis type strain CECT 4600T. Methods and Results: Eighty-two proteins, consistently present in all 2D-gels, were identified by mass spectrometry or by de novo sequencing. The majority of the proteins identified (66%) belonged to four COG categories: 'Carbohydrate transport and metabolism', 'Post-translational modification, protein turnover and chaperones', 'Energy production', and 'Amino acid transport and metabolism'. Glyceraldehyde-3-phosphate dehydrogenase, enolase, fructose-bisphosphate aldolase, phosphoglycerate kinase. molecular chaperones Dnak and GroEL, alkyl hydroperoxide reductase, peptidyl-prolyl cis-trans isomerase B and factor Tu, were identified among the 20 most abundant proteins. A comparison of this reference map with that obtained for the V. tapetis strain GR0202RD, with different origin and pathophysiological characteristics, was performed. Conclusions: Under the culture conditions employed in this study, glucose degradation is one of the major pathways for energy production in Vibrio tapetis. In addition, the two strains studied, although with remarkable differences at genetic and pathophysiological levels, showed a high similarity under laboratory conditions. Significance and Impact of the Study: The results obtained here can be considered as a first step to gather valuable information on protein expression, related not only to diverse cellular functions and regulation but also to pathogenesis and bacterium-host interactions in the disease process

Versatilidad del colgajo safeno para la cobertura de los defectos de rodilla: presentación de 6 casos

Clásicamente las pérdidas de sustancia de la rodilla se resuelven mediante injertos, colgajos cutáneos o fasciocutáneos, o bien por colgajos musculares de gemelo interno o externo, según la localización y profundidad del defecto. El propósito del trabajo es destacar la creciente relevancia de los colgajos neurovaseularizados como el colgajo safeno en la rodilla, en especial para aquellos casos sin gran componente infeccioso, en los que prevalecerían las indicaciones de utilización de colgajos musculares. Presentamos 4 casos de cobertura de prótesis de rodila expuestas en la región de la tuberosidad tibial anterior y/o línea de sutura y otros 2 casos de defectos tras resección tumoral. Los casos han sido realizados durante los 3 últimos años y su seguimiento clínico es de 6 meses a 3 años. En todos los casos se resolvió el problema de cobertura.Traditionally, substance loss at the knee level is solved through skin grafts, cutaneous or fascio-cutaneous flaps or through medial o lateral gastrocnemius muscle flaps depeding on the location and deep of the defect. We highlight the increasing relevance of neurovasculariccd flaps, and specially in this region, of the saphenous flap, better in cases with minor infectious component, in which the indication would be muscular flaps. We present four clinical cases in which we used them to cover anterior tibial tuberosity and/or dehiscent of skin closure and collected during the 3 last years another two cases with cutaneous defect after tumoral resection. All have uneventful recovery. The follow-up period varied from 6 months to 3 years

Assessment of Financial Management Practices of Small and Medium Enterprises (SMEs) in Nasugbu, Batangas

The Small and Medium Enterprises have been identified as important contributors to economic development who are facing a range of challenges which work against their progress, one of which is financial management. This study aimed to assess the financial management practices of small and medium enterprises in Nasugbu, Batangas. The researchers used the descriptive method with a questionnaire as the main instrument in gathering data from 34 respondents. Frequency, percentage, and mean were used as statistical tools in the study, and analysis of variance was used to determine the significant differences between variables. The data gathered were tallied, analyzed and interpreted. The results of the study revealed that majority of the respondents implement financial management practices. Financial management practices such as financial planning, investment, and working capital management are highly applied by small and medium enterprises in Nasugbu, Batangas. It was also revealed that there is a significant difference in the application of financial management practices of small and medium enterprises when they are grouped according to business profile. The outcome of this study may be useful for the entrepreneurs to focus on financial management practices in order to enhance their business performance.

Applications of computational geometry to the molecular simulation of interfaces

The identification of the interfacial molecules in fluid-fluid equilibrium is a long-standing problem in the area of simulation. We here propose a new point of view, making use of concepts taken from the field of computational geometry, where the definition of the "shape" of a set of point is a well-known problem. In particular, we employ the $\alpha$-shape construction which, applied to the positions of the molecules, selects a shape and identifies its boundary points, which we will take to define our interfacial molecules. A single parameter needs to be fixed (the "$\alpha$" of the $\alpha$-shape), and several proposals are examined, all leading to very similar choices. Results of this methodology are evaluated against previous proposals, and seen to be reasonable.Comment: 22 pages, 8 figure

Motile dislocations knead odd crystals into whorls

The competition between thermal fluctuations and potential forces governs the stability of matter in equilibrium, in particular the proliferation and annihilation of topological defects. However, driving matter out of equilibrium allows for a new class of forces that are neither attractive nor repulsive, but rather transverse. The possibility of activating transverse forces raises the question of how they affect basic principles of material self-organization and control. Here we show that transverse forces organize colloidal spinners into odd elastic crystals crisscrossed by motile dislocations. These motile topological defects organize into a polycrystal made of grains with tunable length scale and rotation rate. The self-kneading dynamics drive super-diffusive mass transport, which can be controlled over orders of magnitude by varying the spinning rate. Simulations of both a minimal model and fully resolved hydrodynamics establish the generic nature of this crystal whorl state. Using a continuum theory, we show that both odd and Hall stresses can destabilize odd elastic crystals, giving rise to a generic state of crystalline active matter. Adding rotations to a material’s constituents has far-reaching consequences for continuous control of structures and transport at all scales.The National Science Foundation (NSF) under award no. DMR-2011854. NSF DMR-1905974, NSF EFRI NewLAW 1741685 and the Packard Foundation. NSF grants DMR-1420073 (NYU-MRSEC) and DMR-2004469. ARN grant WTF and IdexLyon Tore. The National Science Foundation Graduate Research Fellowship under grant no. 1746045. D.B. The Chicago-France FACCTS programme. ‘la Caixa’ Foundation (ID 100010434), fellowship LCF/BQ/PI20/11760014 and from the European Union’s Horizon 2020 research and innovation programme under Marie Skłodowska-Curie grant agreement no. 847648. NSF DMR-1828629 and US NSF grant no. DMR-201185

Correlation between Infrared Colors and Intensity Ratios of SiO Maser Lines

We present the results of SiO millimeter-line observations of a sample of known SiO maser sources covering a wide dust-temperature range. A cold part of the sample was selected from the SiO maser sources found in our recent SiO maser survey of cold dusty objects. The aim of the present research is to investigate the causes of the correlation between infrared colors and SiO maser intensity ratios among different transition lines. In particular, the correlation between infrared colors and SiO maser intensity ratio among the J=1-0 v=1, 2, and 3 lines are mainly concerned in this paper. We observed in total 75 SiO maser sources with the Nobeyama 45m telescope quasi-simultaneously in the SiO J=1-0 v=0, 1, 2, 3, 4 and J=2-1 v=1, 2 lines. We also observed the sample in the 29SiO J=1-0 v=0 and J=2-1 v=0, and 30SiO J=1-0 v=0 lines, and the H2O 6(1,6)-5(2,3) line. As reported in previous papers, we confirmed that the intensity ratios of the SiO J=1-0 v=2 to v=1 lines clearly correlate with infrared colors. In addition, we found possible correlation between infrared colors and the intensity ratios of the SiO J=1-0 v=3 to v=1&2 lines. Two overlap lines of H2O (i.e., 11(6,6) nu_2=1 -> 12(7,5) nu_2=0 and 5(0,5) nu_2=2 -> 6(3,4) nu_2=1) might explain these correlation if these overlap lines become stronger with increase of infrared colors, although the phenomena also might be explained by more fundamental ways if we take into account the variation of opacity from object to object.Comment: 49 pages, 7 figures, 3 tables, accepted for publication in ApJ. Full resolution version available at http://www.asiaa.sinica.edu.tw/~junichi/paper

Chronic exposure to cannabinoids during adolescence causes long-lasting behavioral deficits in adult mice

Regular use of marijuana during adolescence enhances the risk of long-lasting neurobiological changes in adulthood. The present study was aimed at assessing the effect of long-term administration of the synthetic cannabinoid WIN55212.2 during adolescence in young adult mice. Adolescent mice aged 5 weeks were subjected daily to the pharmacological action of WIN55212.2 for 3 weeks and were then left undisturbed in their home cage for a 5-week period and finally evaluated by behavioral testing. Mice that received the drug during adolescence showed memory impairment in the Morris water maze, as well as a dose-dependent memory impairment in fear conditioning. In addition, the administration of 3 mg/kg WIN55212.2 in adolescence increased adult hippocampal AEA levels and promoted DNA hypermethylation at the intragenic region of the intracellular signaling modulator Rgs7, which was accompanied by a lower rate of mRNA transcription of this gene, suggesting a potential causal relation. Although the concrete mechanisms underlying the behavioral observations remain to be elucidated, we demonstrate that long-term administration of 3 mg/kg of WIN during adolescence leads to increased endocannabinoid levels and altered Rgs7 expression in adulthood and establish a potential link to epigenetic changes.Beca Ramón y Caja

Staggered Schemes for Fluctuating Hydrodynamics

We develop numerical schemes for solving the isothermal compressible and incompressible equations of fluctuating hydrodynamics on a grid with staggered momenta. We develop a second-order accurate spatial discretization of the diffusive, advective and stochastic fluxes that satisfies a discrete fluctuation-dissipation balance, and construct temporal discretizations that are at least second-order accurate in time deterministically and in a weak sense. Specifically, the methods reproduce the correct equilibrium covariances of the fluctuating fields to third (compressible) and second (incompressible) order in the time step, as we verify numerically. We apply our techniques to model recent experimental measurements of giant fluctuations in diffusively mixing fluids in a micro-gravity environment [A. Vailati et. al., Nature Communications 2:290, 2011]. Numerical results for the static spectrum of non-equilibrium concentration fluctuations are in excellent agreement between the compressible and incompressible simulations, and in good agreement with experimental results for all measured wavenumbers.Comment: Submitted. See also arXiv:0906.242