Stability analysis of apparent resistivity measurement in the seismically active area of Val d'Agri (southern Italy)

International audienceA magnetotelluric monitoring station has been installed in the Val d'Agri area (southern Italy), to investigate the physics underlying the generation mechanisms of the electrokinetic effect, due to rapid pore pressure changes and fluid flows near the focal area of incoming earthquakes. It is well known that the magnetotelluric method reveals variations in electrical resistivity within the Earth at large depths, reaching within appropriate frequency bands the Earth's mantle. Depth sounding is performed by measuring the ratio between the mutually perpendicular horizontal electric and magnetic fields at the earth's surface, furnishing the apparent resistivity, which describes the electrical properties of subsoil as function of depth. The selected site of Val d'Agri has been struck by strong seismic events in past and recent years, this suggesting the investigation of possible changes in apparent resistivity correlated with the local tectonic activity. We analyzed the stability of the measurement of apparent resistivity and phase of the impedance tensor Z(?) during time. Our findings suggest that the measure of apparent resistivity during night-time is more stable. Therefore, we identified the characteristic apparent resistivity curve of the subsoil of the Val d'Agri site, which could be considered as a reference

The Fearful Citizen: Crime and Support for Democracy in Latin America

This article shows that while the impact of crime victimization on support for democracy is sensitive to question wording, the influence of fear of crime on this attitude is consistent and immune to measurement effects. We construe this as evidence that fear of crime has greater attitudinal consequences for democratic support than crime victimization. We show that fear of crime is affected by actual individual and contextual levels of crime victimization as well as evaluations of regime performance. Finally, and consistent with the affective intelligence literature, we find that crime fails to activate people’s surveillance systems in countries that exhibit very low levels of it (typically, where less than 10% of respondents report to have been victims of crime). It is only in countries that have significant crime victimization where fear of it becomes a factor affecting support for democracy.This article shows that while the impact of crime victimization on support for democracy is sensitive to question wording, the influence of fear of crime on this attitude is consistent and immune to measurement effects. We construe this as evidence that fear of crime has greater attitudinal consequences for democratic support than crime victimization. We show that fear of crime is affected by actual individual and contextual levels of crime victimization as well as evaluations of regime performance. Finally, and consistent with the affective intelligence literature, we find that crime fails to activate people’s surveillance systems in countries that exhibit very low levels of it (typically, where less than 10% of respondents report to have been victims of crime). It is only in countries that have significant crime victimization where fear of it becomes a factor affecting support for democracy

Investigating correlations between earthquakes and extreme eventsin self-potential data recorded in a seismicarea of Southestern Appennine Chain (Italy)

The Normalized Wavelet Cross-Correlation Function (NWCCF) was used to study correlations between the series of extreme events in self-potential data and earthquakes, both modelled as stochastic point processes. This method gives objective results, robust to the presence of nonstationarities that often affect observational time series. Furthermore, the NWCCF identifies the timescales involved in the cross-correlated behaviour between two point processes. In particular, we analyzed the cross-correlation between the sequence of extreme events in selfpotential data measured at the monitoring station Tito, located in a seismic area of Southern Italy, and the series of earthquakes which occurred in the same area during 2001. To evaluate the influence of rain on the dynamics of geoelectrical variations, we applied the same approach between the selected extreme values and the rain data. We find that the anomalous geoelectrical values seem to cross-correlate with the rain at short and intermediate timescales (t< 500 h), while they significantly cross-correlate only with earthquakes (M = 2.5) at long timescales (t> 500 h)

Structural and functional analysis of the simultaneous binding of two duplex/quadruplex aptamers to human α-thrombin

: The long-range communication between the two exosites of human α-thrombin (thrombin) tightly modulates the protein-effector interactions. Duplex/quadruplex aptamers represent an emerging class of very effective binders of thrombin. Among them, NU172 and HD22 aptamers are at the forefront of exosite I and II recognition, respectively. The present study investigates the simultaneous binding of these two aptamers by combining a structural and dynamics approach. The crystal structure of the ternary complex formed by the thrombin with NU172 and HD22_27mer provides a detailed view of the simultaneous binding of these aptamers to the protein, inspiring the design of novel bivalent thrombin inhibitors. The crystal structure represents the starting model for molecular dynamics studies, which point out the cooperation between the binding at the two exosites. In particular, the binding of an aptamer to its exosite reduces the intrinsic flexibility of the other exosite, that preferentially assumes conformations similar to those observed in the bound state, suggesting a predisposition to interact with the other aptamer. This behaviour is reflected in a significant increase of the anticoagulant activity of NU172 when the inactive HD22_27mer is bound to exosite II, providing a clear evidence of the synergic action of the two aptamers

Exosite Binding in Thrombin: A Global Structural/Dynamic Overview of Complexes with Aptamers and Other Ligands

: Thrombin is the key enzyme of the entire hemostatic process since it is able to exert both procoagulant and anticoagulant functions; therefore, it represents an attractive target for the developments of biomolecules with therapeutic potential. Thrombin can perform its many functional activities because of its ability to recognize a wide variety of substrates, inhibitors, and cofactors. These molecules frequently are bound to positively charged regions on the surface of protein called exosites. In this review, we carried out extensive analyses of the structural determinants of thrombin partnerships by surveying literature data as well as the structural content of the Protein Data Bank (PDB). In particular, we used the information collected on functional, natural, and synthetic molecular ligands to define the anatomy of the exosites and to quantify the interface area between thrombin and exosite ligands. In this framework, we reviewed in detail the specificity of thrombin binding to aptamers, a class of compounds with intriguing pharmaceutical properties. Although these compounds anchor to protein using conservative patterns on its surface, the present analysis highlights some interesting peculiarities. Moreover, the impact of thrombin binding aptamers in the elucidation of the cross-talk between the two distant exosites is illustrated. Collectively, the data and the work here reviewed may provide insights into the design of novel thrombin inhibitors

A new magnetotelluric monitoring network operating in Agri Valley (Southern Italy): study of stability of apparent resistivity estimates

Variations detected in geophysical, especially electromagnetic, parameters in seismic active areas have been sometimes attributed to modifications of the stress field. Among the different geophysical methods, magnetotellurics (MT) could be one of the most effective because it allows us to explore down to seismogenic depths. Continuous MT recording could allow us to evaluate whether possible variations are significantly correlated with the seismic activity of investigated area. To assess the significance of such observations we must be able to say how well an apparent resistivity curve should be reproduced when measurements are repeated at a later time. To do this properly it is essential to know that the estimated error bars accurately represent the true uncertainties in comparing the transfer functions. In this work we will show the preliminary results obtained from the analysis of the data coming from the new MT monitoring network installed in Agri Valley. This analysis gives us the possibility: i) to better study the temporal stability of the signals, ii) to better discriminate the noise affecting the measures by remote reference estimation. The performed analysis disclosed a relatively low degree of noise in the investigated area, which is a promising condition for monitoring

A new magnetotelluric monitoring network operating in Agri Valley (Southern Italy): study of stability of apparent resistivity estimates

Variations detected in geophysical, especially electromagnetic, parameters in seismic active areas have been sometimes attributed to modifications of the stress field. Among the different geophysical methods, magnetotellurics (MT) could be one of the most effective because it allows us to explore down to seismogenic depths. Continuous MT recording could allow us to evaluate whether possible variations are significantly correlated with the seismic activity of investigated area. To assess the significance of such observations we must be able to say how well an apparent resistivity curve should be reproduced when measurements are repeated at a later time. To do this properly it is essential to know that the estimated error bars accurately represent the true uncertainties in comparing the transfer functions. In this work we will show the preliminary results obtained from the analysis of the data coming from the new MT monitoring network installed in Agri Valley. This analysis gives us the possibility: i) to better study the temporal stability of the signals, ii) to better discriminate the noise affecting the measures by remote reference estimation. The performed analysis disclosed a relatively low degree of noise in the investigated area, which is a promising condition for monitoring

The characterization of Thermotoga maritima Arginine Binding Protein variants demonstrates that minimal local strains have an important impact on protein stability

The Ramachandran plot is a versatile and valuable tool that provides fundamental information for protein structure determination, prediction, and validation. The structural/thermodynamic effects produced by forcing a residue to adopt a conformation predicted to be forbidden were here explored using Thermotoga maritima Arginine Binding Protein (TmArgBP) as model. Specifically, we mutated TmArgBP Gly52 that assumes a conformation believed to be strictly disallowed for non-Gly residues. Surprisingly, the crystallographic characterization of Gly52Ala TmArgBP indicates that the structural context forces the residue to adopt a non-canonical conformation never observed in any of the high-medium resolution PDB structures. Interestingly, the inspection of this high resolution structure demonstrates that only minor alterations occur. Nevertheless, experiments indicate that Gly52 replacements in TmArgBP produce destabilizations comparable to those observed upon protein truncation or dissection in domains. Notably, we show that force-fields commonly used in computational biology do not reproduce this non-canonical state. Using TmArgBP as model system we here demonstrate that the structural context may force residues to adopt conformations believed to be strictly forbidden and that barely detectable alterations produce major destabilizations. Present findings highlight the role of subtle strains in governing protein stability. A full understanding of these phenomena is essential for an exhaustive comprehension of the factors regulating protein structures

Deep electrical resistivity tomography and geothermal analysis of Bradano foredeep deposits in Venosa area (Southern Italy): preliminary results

Geophysical surveys have been carried out to characterize the stratigraphical and structural setting and to better understand the deep water circulation system in the Venosa area (Southern Italy) located in the frontal portion of the southern Appenninic Subduction. In this area there are some deep water wells from which a water conductivity of about 3 mS/cm and a temperature of about 35°C was measured. A deep geoelectrical tomography with dipole-dipole array has been carried out along a profile of 10000 m and an investigation depth of about 900 m. Furthermore a broad band magnetotelluric profile consisting of six stations was performed to infer the resistivity distribution up to some kilometres of depth. The MT profile was almost coincident with the geoelectrical outline. The applied methods allow us to obtain a mutual control and integrated interpretation of the data. The high resolution of the data was the key to reconstruct the structural asset of buried carbonatic horst whose top is located at about 600 m depth. The final results coming from data wells, geothermal analysis and geophysical data, highlighted a horst saturated with salted water and an anomalous local gradient of 60°C/km. The proposed mechanism is that of a mixing of fossil and fresh water circulation system

Magnetotelluric investigation in the High Agri Valley (southern Apennine, Italy)

Abstract. In this paper we present the result of a magnetotelluric (MT) investigation carried out across the High Agri Valley (HAV), southern Italy. Several MT soundings were carried out in order to obtain a ~15 km long 2-D resistivity model with an investigation depth of ~10 km. The main aim was to provide valuable data on the geological and structural setting of the HAV. The MT model was compared with pre-existing geological, geophysical and seismic data. The MT model can be schematized as a superposition of three stack lateral varying layers with different thickness and resistivity values: a surficial low–medium resistivity layer associated with the Quaternary deposits and to the allochthonous units; and a deeper high resistivity layer related to the Apulia Platform, separated by a thin layer connected to the mélange zone and to the Pliocene terrigenous marine deposits. Sharp lateral resistivity variations are interpreted as faults that, on the basis of accurate focal mechanism computations, display normal-faulting kinematics