Testing the SOC hypothesis for the magnetosphere

As noted by Chang, the hypothesis of Self-Organised Criticality provides a theoretical framework in which the low dimensionality seen in magnetospheric indices can be combined with the scaling seen in their power spectra and the recently-observed plasma bursty bulk flows. As such, it has considerable appeal, describing the aspects of the magnetospheric fuelling:storage:release cycle which are generic to slowly-driven, interaction-dominated, thresholded systems rather than unique to the magnetosphere. In consequence, several recent numerical "sandpile" algorithms have been used with a view to comparison with magnetospheric observables. However, demonstration of SOC in the magnetosphere will require further work in the definition of a set of observable properties which are the unique "fingerprint" of SOC. This is because, for example, a scale-free power spectrum admits several possible explanations other than SOC. A more subtle problem is important for both simulations and data analysis when dealing with multiscale and hence broadband phenomena such as SOC. This is that finite length systems such as the magnetosphere or magnetotail will by definition give information over a small range of orders of magnitude, and so scaling will tend to be narrowband. Here we develop a simple framework in which previous descriptions of magnetospheric dynamics can be described and contrasted. We then review existing observations which are indicative of SOC, and ask if they are sufficient to demonstrate it unambiguously, and if not, what new observations need to be made?Comment: 29 pages, 0 figures. Based on invited talk at Spring American Geophysical Union Meeting, 1999. Journal of Atmospheric and Solar Terrestrial Physics, in pres

25 Years of Self-organized Criticality: Concepts and Controversies

Introduced by the late Per Bak and his colleagues, self-organized criticality (SOC) has been one of the most stimulating concepts to come out of statistical mechanics and condensed matter theory in the last few decades, and has played a significant role in the development of complexity science. SOC, and more generally fractals and power laws, have attracted much comment, ranging from the very positive to the polemical. The other papers (Aschwanden et al. in Space Sci. Rev., 2014, this issue; McAteer et al. in Space Sci. Rev., 2015, this issue; Sharma et al. in Space Sci. Rev. 2015, in preparation) in this special issue showcase the considerable body of observations in solar, magnetospheric and fusion plasma inspired by the SOC idea, and expose the fertile role the new paradigm has played in approaches to modeling and understanding multiscale plasma instabilities. This very broad impact, and the necessary process of adapting a scientific hypothesis to the conditions of a given physical system, has meant that SOC as studied in these fields has sometimes differed significantly from the definition originally given by its creators. In Bak’s own field of theoretical physics there are significant observational and theoretical open questions, even 25 years on (Pruessner 2012). One aim of the present review is to address the dichotomy between the great reception SOC has received in some areas, and its shortcomings, as they became manifest in the controversies it triggered. Our article tries to clear up what we think are misunderstandings of SOC in fields more remote from its origins in statistical mechanics, condensed matter and dynamical systems by revisiting Bak, Tang and Wiesenfeld’s original papers

Escherichia coli RuvBL268S: A mutant RuvB protein that exhibits wild-type activities in vitro but confers a UV-sensitive ruv phenotype in vivo

The RuvABC proteins of Escherichia coli process recombination intermediates during genetic recombination and DNA repair. RuvA and RuvB promote branch migration of Holliday junctions, a process that extends heteroduplex DNA. Together with RuvC, they form a RuvABC complex capable of Holliday junction resolution. Branch migration by RuvAB is mediated by RuvB, a hexameric ring protein that acts as an ATP-driven molecular pump. To gain insight into the mechanism of branch migration, random mutations were introduced into the ruvB gene by PCR and a collection of mutant alleles were obtained. Mutation of leucine 268 to serine resulted in a severe UV-sensitive phenotype, characteristic of a ruv defect. Here, we report a biochemical analysis of the mutant protein RuvBL268S. Unexpectedly, the purified protein is fully active in vitro with regard to its ATPase, DNA binding and DNA unwinding activities. It also promotes efficient branch migration in combination with RuvA, and forms functional RuvABC-Holliday junction resolvase complexes. These results indicate that RuvB may perform some additional, and as yet undefined, function that is necessary for cell survival after UV-irradiatio

Comparative genomic integration profiling of Sleeping Beauty transposons mobilized with high efficacy from integrase-defective lentiviral vectors in primary human cells

It has been previously shown that integrase-defective HIV-1-based gene vectors can serve, with moderate efficiency, as substrate for DNA transposition by a transiently expressed Sleeping Beauty (SB) transposase. Here, we describe the enhanced gene transfer properties of a HIV-1/SB hybrid vector that allows efficient DNA transposition, facilitated by the hyperactive SB100X transposase, from vector DNA intermediates in primary human cells. Potent transposase-dependent integration of genetic cargo carried by the hybrid HIV-1/SB vector (up to 160-fold above background) is reported in human cell lines as well as in primary human fibroblasts and keratinocytes. The efficiency of transgene integration in context of the newly developed hybrid vector is comparable with that of conventional lentiviral vectors (LVs). Integration profiles of integrating HIV-1-derived vectors and SB transposons mobilized from LVs are investigated by deep sequencing of a large number of integration sites. A significant bias of lentiviral integrations in genes is reported, confirming that biological properties of the viral integration machinery facilitate preferred insertion into actively transcribed genomic regions. In sharp contrast, lentiviral insertions catalyzed by the SB100X transposase are far more random with respect to genes. Based on these properties, HIV-1/SB vectors may become valuable tools for genetic engineering and therapeutic gene transfer

Pain perception and brain evoked potentials in patients with angina despite normal coronary angiograms.

OBJECTIVE: To evaluate the role of nociception in patients with angina despite normal coronary angiograms and to investigate whether any abnormality is confined to visceral or somatosensory perception. METHODS: Perception, pain threshold, and brain evoked potentials to nociceptive electrical stimuli of the oesophageal mucosa and the sternal skin were investigated in 10 patients who had angina but normal coronary angiograms, no other signs of cardiac disease, and normal upper endoscopy. Controls were 10 healthy volunteers. The peaks of the evoked potential signal were designated N for negative deflections and P for positive. Numbers were given to the peaks in order of appearance after the stimulus. The peak to peak amplitudes (P1/N1, N1/P2) were measured in microV. RESULTS: (1) Angina pectoris was provoked in seven patients following continuous oesophageal stimulation. (2) Distant projection of pain occurred after continuous electrical stimulation of the oesophagus in four patients and in no controls. (3) Patients had higher oesophageal pain thresholds (median 16.3 mA v 7.3 mA, P = 0.02) to repeated stimuli than controls, whereas the values did not differ with respect to the skin. There were no intergroup differences in thresholds to single stimuli. (4) Patients had substantially reduced brain evoked potential amplitudes after both single oesophageal (P1/N1, median values: 7.2 microV, controls: 29.0 microV; N1/P2: 16.5 microV, controls: 66.0 microV; P < 0.001 for both) and skin (N1/P2: 13.5 microV; controls: 76.0 microV; P < 0.001) stimuli despite the similar pain thresholds. CONCLUSION: Central nervous system responses to visceral and somatosensory nociceptive input are altered in patients who have angina despite normal coronary angiograms

Nonclassical transport and particle-field coupling : from laboratory plasmas to the solar wind

Understanding transport of thermal and suprathermal particles is a fundamental issue in laboratory, solar-terrestrial, and astrophysical plasmas. For laboratory fusion experiments, confinement of particles and energy is essential for sustaining the plasma long enough to reach burning conditions. For solar wind and magnetospheric plasmas, transport properties determine the spatial and temporal distribution of energetic particles, which can be harmful for spacecraft functioning, as well as the entry of solar wind plasma into the magnetosphere. For astrophysical plasmas, transport properties determine the efficiency of particle acceleration processes and affect observable radiative signatures. In all cases, transport depends on the interaction of thermal and suprathermal particles with the electric and magnetic fluctuations in the plasma. Understanding transport therefore requires us to understand these interactions, which encompass a wide range of scales, from magnetohydrodynamic to kinetic scales, with larger scale structures also having a role. The wealth of transport studies during recent decades has shown the existence of a variety of regimes that differ from the classical quasilinear regime. In this paper we give an overview of nonclassical plasma transport regimes, discussing theoretical approaches to superdiffusive and subdiffusive transport, wave-particle interactions at microscopic kinetic scales, the influence of coherent structures and of avalanching transport, and the results of numerical simulations and experimental data analyses. Applications to laboratory plasmas and space plasmas are discussed