MICROBIAL CHARACTERIZATION OF ABANDONED MINING AREA OF SIDI KAMBER NORTH-EAST OF ALGERI

The ecological importance of soil bacteria is not limited to their number or biomass, although these parameters contribute greatly. Indeed, their main asset lies in their great genetic and functional diversity. In this study carried out in the abandoned mine, located in Sidi Kamber (Oum Toub, Skikda, North-Est of Algeria) our objectives was to determine: a) contamination levels of this area by heavy metals b) heavy metal impact on bacterial communities and diversity c) possible risk on the ecological integrity of this area

A binary system of complementizers in Cimbrian relative clauses

The system of Cimbrian relative clauses manifests itself in a complex scenario: two different complementizers occur in this context: i) the ‘autochthonous’ (Germanic) bo, cognate of Southern German wo, and ii) the ‘allochthonous’ ke, borrowed from Italian (che), which is gradually spreading. In our paper we provide empirical evidence for a crucial specialization of both complementizers: the former shows up only in restrictive relative clauses, the latter in both restrictive and non-restrictive relatives, giving rise to a binary system. In our analysis we aim to explain the binary system of Cimbrian relative complementizers directly addressing the general discussion about relative clauses, showing once more the relevance of both linguistic contact and microvariation for the theory of grammar

Development of the Innate Immune Response in NestlingTree Swallows (Tachycineta bicolor)

The innate immune system provides an immediate, short term, first line of defense from pathogens; its appearance early in development in vertebrates is evidence of its critical importance. Even so, few studies have investigated the development of the immune response as juveniles transition into adults. Ultimately, the ability to respond to pathogens confers fitness benefits in terms of health, survival, and reproductive success, and it follows that functions such as rapid growth cannot be fully met simultaneously since energy is a limiting resource. As a result, defense mechanisms are compromised at an early age due to energy allocation to rapid growth; therefore, immunity should increase as individuals mature. I studied the development of innate immunity in nestling Tree Swallows using microbicidal assays which were conducted in vitro to assess the ability of the immune system to kill E. coli via lysis. This research may provide insight into patterns of disease susceptibility, which in turn influence evolutionary fitness and population dynamics

Aptamer targeting of the elongation factor 1A impairs hepatocarcinoma cells viability and potentiates bortezomib and idarubicin effects

8noThe high morbidity and mortality of hepatocellular carcinoma (HCC) is mostly due to the limited efficacy of the available therapeutic approaches. Here we explore the anti-HCC potential of an aptamer targeting the elongation factor 1A (eEF1A), a protein implicated in the promotion of HCC. As delivery methods, we have compared the effectiveness of cationic liposome and cholesterol-mediated approaches. A75 nucleotide long aptamer containing GT repetition (GT75) was tested in three HCC cell lines, HepG2, HuH7 and JHH6. When delivered by liposomes, GT75 was able to effectively reducing HCC cells viability in a dose and time dependent fashion. Particular sensitive were JHH6 where increased apoptosis with no effects on cell cycle were observed. GT75 effect was likely due to the interference with eEF1A activity as neither the mRNA nor the protein levels were significantly affected. Notably, cholesterol-mediated delivery of GT75 abrogated its efficacy due to cellular mis-localization as proven by fluorescence and confocal microscopic analysis. Finally, liposome-mediated delivery of GT75 improved the therapeutic index of the anticancer drugs bortezomib and idarubicin. In conclusion, liposome but not cholesterol-mediated delivery of GT75 resulted in an effective delivery of GT75, causing the impairment of the vitality of a panel of HCC derived cells.partially_openopenScaggiante, Bruna; Farra, Rossella; Dapas, Barbara; Baj, Gabriele; Pozzato, Gabriele; Grassi, Mario; Zanconati, Fabrizio; Grassi, GabrieleScaggiante, Bruna; Farra, Rossella; Dapas, Barbara; Baj, Gabriele; Pozzato, Gabriele; Grassi, Mario; Zanconati, Fabrizio; Grassi, Gabriel

Ictiólogos de la Argentina : Sarah Exilda Cabrera

Fil: López, Hugo Luis. División Zoología Vertebrados; Facultad de Ciencias Naturales y Museo; Universidad Nacional de La PlataFil: Ponte Gómez, Justina. División Zoología Vertebrados; Facultad de Ciencias Naturales y Museo; Universidad Nacional de La Plat