Orion Power Needs

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RNA interference : a promising biopesticide strategy against the African sweetpotato weevil Cylas brunneus

The African sweetpotato weevil Cylas brunneus is one of the most devastating pests affecting the production of sweetpotatoes, an important staple food in Sub-Saharan Africa. Current available control methods against this coleopteran pest are limited. In this study, we analyzed the potential of RNA interference as a novel crop protection strategy against this insect pest. First, the C. brunneus transcriptome was sequenced and RNAi functionality was confirmed by successfully silencing the laccase2 gene. Next, 24 potential target genes were chosen, based on their critical role in vital biological processes. A first screening via injection of gene-specific dsRNAs showed that the dsRNAs were highly toxic for C. brunneus. Injected doses of 200ng/mg body weight led to mortality rates of 90% or higher for 14 of the 24 tested genes after 14 days. The three best performing dsRNAs, targeting pros alpha 2, rps13 and the homolog of Diabrotica virgifera snf7, were then used in further feeding trials to investigate RNAi by oral delivery. Different concentrations of dsRNAs mixed with artificial diet were tested and concentrations as low as 1 mu g dsRNA/mL diet led to significant mortality rates higher than 50%. These results proved that dsRNAs targeting essential genes show great potential to control C. brunneus

Single Event Effects Testing For Low Earth Orbit Missions with Neutrons

Neutrons can effectively be used to screen electronic parts intended to be used in Low Earth Orbit. This paper compares neutron with proton environments in spacecraft and discusses recent comparison testing

The Grizzly, October 14, 1988

Task Force Committee Trims Curriculum • Gender, Class and Race Addressed at Conference • Awareness Week on the Wagon • Letters: Some Sobering Thoughts for Students; Interdepartmental Cooperation Stressed • And the Band Played On... • A Voice of My Own to Air • Wellness Week Results • Rice Cooks Up Recipe for Art • Bears Take Homecoming Thriller in Final :33 • Soccer Kicks Into Winning Gear • X-Country Runs \u27Round Alumni • Hockey Unpredictablehttps://digitalcommons.ursinus.edu/grizzlynews/1220/thumbnail.jp

The Grizzly, October 7, 1988

Berman Art Center Breaks Ground • Pledging Undergoes Changes • Welcome Home Alumni! • Letter: Honor Code Discussion Revived • Giving Garbage the Dump • Jazz: Basie Style • Ursinus Presents: A Voice of My Own • On the Forum Front • Mistake Free Bears Get First Win • Field Hockey Making Gains • Soccer Gains Respect • Annual Run Offers Health and Fun • 1988 Homecoming Queen Candidates • Sherman Strutting Stuff • Intramural Results Announced • U.C. Welcomes Gilbert\u27s Enthusiasm • Cycling Club Returns • Campbell: Not Your Typical Bowl of Soup • Econ: Economopolis • Professor LoBue Introduces New Chemistry in Pfahler • Discover Discover! • U.C. Students Study Better Late Than Neverhttps://digitalcommons.ursinus.edu/grizzlynews/1219/thumbnail.jp

Prospectus, September 23, 1981

CANDIDATES VOICE THEIR VIEWS; News In Brief; Parkland P adds to landscape; Editors voice opinions; Homeowners can save; WPCD to broadcast football; Football plan goes into effect; New club formed at Parkland; U of I to study special lottery; Scholarships offered to women; Free seminar offered; Vietnam vets made more; Students may still sign up for insurance; Neil Simon\u27s hit musical showing soon; PC offers 3-hour telecourse; Create your own decorations; Classifieds; Beware of Matt\u27s addiction ; McNichol, Hamill star in Georgia ; Two top bands to perform at ISU; C-U Symphony kicks off season Saturday; Assembly Hall offers ticket deals; LRC helps students and faculty; Area\u27s next cash crop?: Some farmers try sunflowers; Mark predicts election results; Drug from sea may hold new hope for cancer and herpes; Newhart to visit C-U area; Speech team needs help; Record shops may become obsolete; VB team loses opener; ...but wins 2nd game; Parkland College Basketball Schedule -- Women 1981-82; Golf team wins against Danville; Sports Notes; Cross Country team off to running start; Airsho offers good timehttps://spark.parkland.edu/prospectus_1981/1012/thumbnail.jp

The Grizzly, September 12, 2000

Student May Lose Squatting Rights, Residence Life Says • Students Peeved at Parking Problems • Freshman Class Elects Student Body Politic • UC Student Organizations Come Alive at Activities Fair • U.S. News Ranks UC High • Freshmen React: New Laptop Receives Praise, Criticism • A PANDA in Bear Country: Local Citizens Fight Against Plans for New Power Plant • In the Ad Campaign, Uber Rocks the Vote! • Letters to the Editor • Editorials: Cutting Education Short; Apathy Lay Dying • Are Freshmen Working Too Hard? • Opinion: Campaign 2000 Presidential Debates • Rugby Looks to Have Tons of Fun in Fifth Season • Field Hockey Still Looking for First Win • Men\u27s Soccer: Win Over Wesley, Shut Out vs. Scranton • Women\u27s Soccer Splits at Pepsi Cardinal Classic • Athletes of the Week: Scott Hussey; Nicole DiMascio • Bears Ground Flying Dutchmen in 39-6 Romp • XC off to Fast Starthttps://digitalcommons.ursinus.edu/grizzlynews/1471/thumbnail.jp

Identification and Molecular Characterization of the Switchgrass AP2/ERF Transcription Factor Superfamily, and Overexpression of PvERF001 for Improvement of Biomass Characteristics for Biofuel

The APETALA2/ethylene response factor (AP2/ERF) superfamily of transcription factors (TFs) plays essential roles in the regulation of various growth and developmental programs including stress responses. Members of these TFs in other plant species have been implicated to play a role in the regulation of cell wall biosynthesis. Here, we identified a total of 207 AP2/ERF TF genes in the switchgrass genome and grouped into four gene families comprised of 25 AP2-, 121 ERF-, 55 DREB (dehydration responsive element binding)-, and 5 RAV (related to API3/VP) genes, as well as a singleton gene not fitting any of the above families. The ERF and DREB subfamilies comprised seven and four distinct groups, respectively. Analysis of exon/intron structures of switchgrass AP2/ERF genes showed high diversity in the distribution of introns in AP2 genes versus a single or no intron in most genes in the ERF and RAV families. The majority of the subfamilies or groups within it were characterized by the presence of one or more specific conserved protein motifs. In silico functional analysis revealed that many genes in these families might be associated with the regulation of responses to environmental stimuli via transcriptional regulation of the response genes. Moreover, these genes had diverse endogenous expression patterns in switchgrass during seed germination, vegetative growth, flower development, and seed formation. Interestingly, several members of the ERF and DREB families were found to be highly expressed in plant tissues where active lignification occurs. These results provide vital resources to select candidate genes to potentially impart tolerance to environmental stress as well as reduced recalcitrance. Overexpression of one of the ERF genes (PvERF001) in switchgrass was associated with increased biomass yield and sugar release efficiency in transgenic lines, exemplifying the potential of these TFs in the development of lignocellulosic feedstocks with improved biomass characteristics for biofuels