Chapter Phytoplankton assemblage Characterization along the Mediterranean coast of Morocco during autumn

The present study aimed to assess the composition, abundance and diversity of phytoplankton assemblage along the Moroccan Mediterranean Coast. Phytoplankton samples were collected in October 2018 at 48 stations from M’diq bay in the West to Saïdia in the East. 92 taxa have been inventoried, belonging to five groups. Diatoms dominate qualitatively and quantitatively (85.5%), followed by dinoflagellates (12%). Phytoplanktonic diversity and abundance was pronounced in the western part of Moroccan Mediterranean Sea, especially (from Jebha to M’diq) because of the influence of the Atlantic flow

Spatial and seasonal fluctuations of Ichthyoplankton assemblage in the Mediterranean coast of Morocco (Southwestern Alboran Sea)

Ichthyoplankton represent the first life stages of fish. The study of ichthyoplankton is crucial to understanding marine ecosystems and plays an important role in the management and durability of fisheries resources. During March and October of 2019, two oceanographic ichthyoplankton surveys were conducted in the Mediterranean Sea of Morocco from Tanger to Saadia by studying the horizontal structure of the ichthyoplankton species assemblage and its relation to environmental parameters. The average surface water temperature was (15.8°C in spring and 16.4°C in autumn). The fish eggs and larvae were more abundant in March than in October (21268 eggs/10m² and 14084 larvae/10m² in spring and 10094 eggs/10m² and 13796 larvae/10m²). In both seasons, fish eggs from the families Sternoptychidae and Sparidae were dominant (10101 eggs/10m² and 7527 eggs/10m² in spring and 4422 eggs/10m² and 3928 eggs/10m² in fall, respectively). However, Myctophidae larvae were the most abundant in the study area, reaching 7601 larvae/10m² in spring and 11021 larvae/10m² in autumn. The environmental parameters: temperature, salinity and chlorophyll-a (surface) seem to directly influence the spatial distribution of ichtyoplancton. On the other hand, it seems that predation by jellyfish (Pelagia noctiluca)was a very important factor that added to the factors that influenced the distribution of the species of fish eggs and larvae. This work represents the first survey conducted in the southwestern Alboran Sea, which studies the horizontal structure of the ichthyoplankton species assemblage and its relation to environmental factors in the spring and autumn of 2019

Variabilite Oceanographique De La Zone D’el Jadida – Jorf Lasfar (Maroc)

Une campagne océanographique côtière a été réalisée en Juillet 2018 entre El Jadida et Jorf Lasfar. Cette zone est caractérisée par la présence d'algues rouges et présente une bathymétrie très régulière de la côte à l’isobathe 20m. Les paramètres océanographiques collectés au cours de cette mission montrent que la colonne d'eau est bien stratifiée avec des eaux froides riches en nutriments au fond et relativement plus chaudes et déficientes en nutriments en surface. La zone étudiée est très influencée par la forte concentration des phosphates provenant des rejets de Jorf Lasfar. Durant la période de la campagne, la stratification verticale pourrait s’expliquer par la faible activité des résurgences des eaux froides profondes. Malgré cette stratification, la colonne d'eau était bien saturée en oxygène dissous aussi bien en surface qu’au fond. A coastal oceanographic cruise was carried out on July 2018 between El Jadida and Jorf Lasfar. This area is characterized by the presence of red algae and presents a very regular bathymetry from the coast to isobaths 20m. The collected oceanographic parameters during this cruise show that the water column is well stratified with cold waters rich in nutrients at the bottom and relatively warmer and deficient in nutrients on the surface. The area study is very influenced by the high concentration of phosphates originated from Jorf Lasfar. During this summer’s period, the vertical stratification could be explained by the low activity of the resurgences of cold deep waters. Despite this stratification, the water column is well saturated on dissolved oxygen both on the surface and at the bottom

Circulation Marine de la Baie de Cintra (Sud du Maroc) par Modèle Hydrodynamique 2D

The marine circulation of Cintra bay located at Dakhla Oued Eddahab area (south of Morocco 23°08’N-16°20’W) has been studied from October to November 2016 by 2D hydrodynamic model. This study aims to understand the marine circulation of this bay and its hydrodynamic functioning. Mainly governed by wind and semidiurnal M2 tides, currents are generally strong in intensity (mainly > 0.5 m.s-1 ) outside the bay. Inside the bay, their intensity are less than 0.3 m.s-1 and generally oriented to the south of the bay due to the influence of the trade winds (NE). Due sometimes to the influence of W and/or SW winds, the coastal circulation of the bay is oriented from the south to the north, impacting its northern part

Etat Oceanographique De La Lagune De Oualidia, Maroc (2011-2012)

The study of the Oualidia lagoon aims to establish the state of knowledge of the environment of this ecosystem after the construction of an inner dam in 2011 and the ecological functioning of the lagoon. For that, several campaigns were carried out between February 2011 and October 2012 and were the subject of a seasonal monitoring for the collection of the main biotic and abiotic parameters of the environment of this ecosystem. During the construction of the inner dam on 2011, a hydrological variability was marked in summer by low oxygen levels and maximum temperature variation between the two tides. Since the completion of the construction of the inner dam, there has been a significant increase in nutrients, mainly nitrogenous elements, and an increase in turbidity caused by an improvement in hydrodynamics, which has particularly increased of the sandpit modified the morphology and moved on south, creating a new main channel structure in 2011. This hydrodynamic was manifested on the upstream area by a decrease of the sedimentary organic matter due to the trapping of fine sediments in the inner dam. At the immediate level of the upstream area (Slikkes and Schorres), the hydrodynamic is relatively low, which has reduced the sedimentation rate on the upstream of the lagoon. The Oualidia lagoon is situated in a littoral zone influenced by the enrichment in deep cold waters loaded with nutritive salts, which invade it during the high tide, and would be, consequently after construction of the inner dam, protected from any confinement of the ecosystem

Comparison and Validation of Ichthyoplankton DNA Extraction Methods

Ichthyoplankton is the cluster of planktonic organisms that consists of fish eggs and larvae. These planktonic stages belong to the temporary zooplankton, representing future exploitable stocks. The study of the early ontogenesis of fish plays a key role in the understanding and evaluation of these populations through the study of their abundance and their spatio-temporal distribution. To better understand and protect these fisheries resources, it is essential to identify the different stages of fish embryonic development. This identification is usually performed using the classical method, based on morphological criteria under a binocular magnifying glass; however, this methodology is not always sufficient and is time consuming and, therefore, it is necessary to rely increasingly on molecular tools. The major problem with these tools is the yield and quality of the nucleic acids extracted from ichthyoplankton, especially in the case of eggs, which are small. Several methods have been used for DNA extraction from ichthyoplankton, either automated or manual, but very often from larvae or adults. In the present work, five fish egg DNA extraction protocols were compared based on their DNA yield and extraction quality, verified by agarose gel electrophoresis and quantitative PCR amplification. The results showed that extraction by our heat-protocol for direct PCR (Hp-dPCR) presents the simplest and cheapest protocol of all the kits used in this study, providing a sufficient quantity and quality of nucleic acids to be used for PCR amplification, and being within the reach of third world laboratories that often do not have sufficiently large budgets to obtain automated kits

Comparative molecular analysis of evolutionarily distant glyceraldehyde-3-phosphate dehydrogenase from Sardina pilchardus and Octopus vulgaris

The NAD+-dependent cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12), which is recognized as a key to central carbon metabolism in glycolysis and gluconeogenesis and as an important allozymic polymorphic biomarker, was purified from muscles of two marine species: the skeletal muscle of Sardina pilchardus Walbaum (Teleost, Clupeida) and the incompressible arm muscle of Octopus vulgaris (Mollusca, Cephalopoda). Comparative biochemical studies have revealed that they differ in their subunit molecular masses and in pI values. Partial cDNA sequences corresponding to an internal region of the GapC genes from Sardina and Octopus were obtained by polymerase chain reaction using degenerate primers designed from highly conserved protein motifs. Alignments of the deduced amino acid sequences were used to establish the 3D structures of the active site of two enzymes as well as the phylogenetic relationships of the sardine and octopus enzymes. These two enzymes are the first two GAPDHs characterized so far from teleost fish and cephalopod, respectively. Interestingly, phylogenetic analyses indicated that the sardina GAPDH is in a cluster with the archetypical enzymes from other vertebrates, while the octopus GAPDH comes together with other molluscan sequences in a distant basal assembly closer to bacterial and fungal orthologs, thus suggesting their different evolutionary scenarios. © 2010 The Author.Peer Reviewe

Short-term variation of zooplankton community in Cintra Bay (Northwest Africa)

Summary: This is the first study that describes the spatial-temporal distributions of the zooplankton community and potential control factors in Cintra Bay. Zooplankton were sampled using a bongo net, 150 μm mesh size, during two surveys, in autumn 2015 and spring 2016, extending from the coast to the open sea. Fourteen zooplankton groups were identified, where copepods represented 49.1% and 92.5% of the total abundance in autumn 2015 and spring 2016, respectively. Tintinnids accounted for 39.7% and 4.7%, respectively. The total zooplankton abundance was higher in autumn (55 992 ind m−3) than in spring (2123 ind m−3). Nineteen species of copepods, belonging to 14 families, were identified. Euterpina acutifrons and Oithona nana were the most common and abundant species. The Acartiids were represented by three species (Acartia clausi, A. tonsa and A. bifilosa) in autumn and one species in spring (A. clausi). The copepods diversity was significantly different between the two seasons showing high values at the entrance and the center of the bay in autumn 2015 and in the southern half of the bay in spring 2016. The copepod structure was characterized by 13 species at different degrees of contribution in autumn 2015. In spring 2016, only four species qualified as indicator species although their contribution was not significant. Given its large opening on the ocean, Cintra Bay benefits largely from the conditions of the oceanic environment, particularly the upwelling. This situation is likely to have an impact on the spatiotemporal variability of the composition and distribution of zooplankton, especially the copepods. Keywords: Cintra Bay, Zooplankton, Copepods, Diversity indices, Environmental parameter

Chapter Phytoplankton assemblage Characterization along the Mediterranean coast of Morocco during autumn

The present study aimed to assess the composition, abundance and diversity of phytoplankton assemblage along the Moroccan Mediterranean Coast. Phytoplankton samples were collected in October 2018 at 48 stations from M’diq bay in the West to Saïdia in the East. 92 taxa have been inventoried, belonging to five groups. Diatoms dominate qualitatively and quantitatively (85.5%), followed by dinoflagellates (12%). Phytoplanktonic diversity and abundance was pronounced in the western part of Moroccan Mediterranean Sea, especially (from Jebha to M’diq) because of the influence of the Atlantic flow