65 research outputs found

    Environmental pollutants modulate RNA and DNA virus-activated miRNA-155 expression and innate immune system responses: Insights into new immunomodulative mechanisms

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    Many persistent organic pollutants, such as polychlorinated biphenyls (PCBs), have high immunomodulating potentials. Exposure to them, in combination with virus infections, has been shown to aggravate outcomes of the infection, leading to increased viral titers and host mortality. Expression of immune-related microRNA (miR) signaling pathways (by host and/or virus) have been shown to be important in determining these outcomes; there is some evidence to suggest pollutants can cause dysregulation of miRNAs. It was thus hypothesized here that modulation of miRNAs (and associated cytokine genes) by pollutants exerts negative effects during viral infections. To test this, an in vitro study on chicken embryo fibroblasts (CEF) exposed to a PCB mixture (Aroclor 1260) and then stimulated with a synthetic RNA virus (poly(I:C)) or infected with a lymphoma-causing DNA virus (Gallid Herpes Virus 2 [GaHV-2]) was conducted. Using quantitative real-time PCR, expression patterns for mir-155, pro-inflammatory TNFα and IL-8, transcription factor NF-κB1, and anti-inflammatory IL-4 were investigated 8, 12, and 18 h after virus activation. The study showed that Aroclor1260 modulated mir-155 expression, such that a down-regulation of mir-155 in poly(I:C)-treated CEF was seen up to 12 h. Aroclor1260 exposure also increased the mRNA expression of pro-inflammatory genes after 8 h in poly(I:C)-treated cells, but levels in GaHV-2-infected cells were unaffected. In contrast to with Aroclor1260/poly(I:C), Aroclor1260/GaHV-2-infected cells displayed an increase in mir-155 levels after 12 h compared to levels seen with either individual treatment. While after 12 h expression of most evaluated genes was down-regulated (independent of treatment regimen), by 18 h, up-regulation was evident again. In conclusion, this study added evidence that mir-155 signaling represents a sensitive pathway to chemically-induced immunomodulation and indicated that PCBs can modulate highly-regulated innate immune system signaling pathways important in determining host immune response outcomes during viral infections.publishedVersio

    Towards harmonisation of chemical monitoring using avian apex predators: identification of key species for pan-European biomonitoring

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    Biomonitoring in raptors can be used to study long-term and large-scale changes in environmental pollution. In Europe, such monitoring is needed to assess environmental risks and outcomes of chemicals regulation, which is harmonised across the European Union. To be effective, the most appropriate sentinels need to be monitored. Our aim was to identify which European raptor species are the likely most appropriate biomonitors when pollutant quantification is based on analysing tissues. Our current study was restricted to terrestrial exposure pathways and considered four priority pollutant groups: toxic metals (lead and mercury), anticoagulant rodenticides, pesticides and medicinal products. We evaluated information on the distribution and key ecological traits (food web, foraging trait, diet, preferred habitat, and migratory behaviour) of European raptors to identify the most appropriate sentinel species. Common buzzard (Buteo buteo) and/or tawny owl (Strix aluco) proved the most suitable candidates for many of the pollutants considered. Moreover, they are abundant in Europe, enhancing the likelihood that samples can be collected. However, other species may be better sentinels for certain pollutants, such as the golden eagle (Aquila chrysaetos) for lead, the northern goshawk (Accipiter gentilis) for mercury across areas including Northern Europe, and vultures (where they occur in Europe) are likely best suited for monitoring non-steroidal anti-inflammatory drugs (NSAIDs). Overall, however, we argue the selection of candidate species for widescale monitoring of a range of pollutants can be reduced to very few raptor species. We recommend that the common buzzard and tawny owl should be the initial focus of any pan-European raptor monitoring. The lack of previous widespread monitoring using these species suggests that their utility as sentinels for environmnetal pollution has not been widely recognised. Finally, although the current study focussed on Europe, our trait-based approach for identifying raptor biomonitors can be applied to other continents and contaminants

    The time-scales probed by star formation rate indicators for realistic, bursty star formation histories from the FIRE simulations

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    Understanding the rate at which stars form is central to studies of galaxy formation. Observationally, the star formation rates (SFRs) of galaxies are measured using the luminosity in different frequency bands, often under the assumption of a time-steady SFR in the recent past. We use star formation histories (SFHs) extracted from cosmological simulations of star-forming galaxies from the FIRE project to analyze the time-scales to which the Hα{\alpha} and far-ultraviolet (FUV) continuum SFR indicators are sensitive. In these simulations, the SFRs are highly time variable for all galaxies at high redshift, and continue to be bursty to z=0 in dwarf galaxies. When FIRE SFHs are partitioned into their bursty and time-steady phases, the best-fitting FUV time-scale fluctuates from its ~10 Myr value when the SFR is time-steady to >~100 Myr immediately following particularly extreme bursts of star formation during the bursty phase. On the other hand, the best-fitting averaging time-scale for Hα{\alpha} is generally insensitive to the SFR variability in the FIRE simulations and remains ~5 Myr at all times. These time-scales are shorter than the 100 Myr and 10 Myr time-scales sometimes assumed in the literature for FUV and Hα{\alpha}, respectively, because while the FUV emission persists for stellar populations older than 100 Myr, the time-dependent luminosities are strongly dominated by younger stars. Our results confirm that the ratio of SFRs inferred using Hα{\alpha} vs. FUV can be used to probe the burstiness of star formation in galaxies.Comment: 14 pages, 10 figures, accepted to MNRA

    Identical or fraternal twins? The chemical homogeneity of wide binaries from Gaia DR2

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    One of the high-level goals of Galactic archaeology is chemical tagging of stars across the Milky Way to piece together its assembly history. For this to work, stars born together must be uniquely chemically homogeneous. Wide binary systems are an important laboratory to test this underlying assumption. Here, we present the detailed chemical abundance patterns of 50 stars across 25 wide binary systems comprised of main-sequence stars of similar spectral type identified in Gaia DR2 with the aim of quantifying their level of chemical homogeneity. Using high-resolution spectra obtained with McDonald Observatory, we derive stellar atmospheric parameters and precise detailed chemical abundances for light/odd-Z (Li, C, Na, Al, Sc, V, Cu), α (Mg, Si, Ca), Fe-peak (Ti, Cr, Mn, Fe, Co, Ni, Zn), and neutron capture (Sr, Y, Zr, Ba, La, Nd, Eu) elements. Results indicate that 80 per cent (20 pairs) of the systems are homogeneous in [Fe/H] at levels below 0.02 dex. These systems are also chemically homogeneous in all elemental abundances studied, with offsets and dispersions consistent with measurement uncertainties. We also find that wide binary systems are far more chemically homogeneous than random pairings of field stars of similar spectral type. These results indicate that wide binary systems tend to be chemically homogeneous but in some cases they can differ in their detailed elemental abundances at a level of [X/H] ∼ 0.10 dex, overall implying chemical tagging in broad strokes can work.KH has been partially supported by a TDA/Scialog grant funded by the Research Corporation and a Scialog grant funded by the Heising-Simons Foundation. KH acknowledges support from the National Science Foundation grant AST-1907417. This work was performed, in part, at the Aspen Center for Physics, which is supported by National Science Foundation grant PHY-1607611. DK and MT have been supported by Cox Endowment funds through the Board of Visitors of the University of Texas at Austin Department of Astronomy. YST is grateful to be supported by the NASA Hubble Fellowship grant HST-HF2-51425.001 awarded by the Space Telescope Science Institute. APJ is supported by NASA through Hubble Fellowship grant HST-HF2-51393.001 awarded by the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., for NASA, under contract NAS5-26555. Support for this work was provided by NASA through Hubble Fellowship grant HST-HF251399.001 awarded to JT by the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., for NASA, under contract NAS5-26555. AC thanks the LSSTC Data Science Fellowship Program, which is funded by LSSTC, NSF Cybertraining Grant 1829740, the Brinson Foundation, and the Moore Foundation. Her participation in the program has benefited this work

    Hot-mode accretion and the physics of thin-disc galaxy formation

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    We use FIRE simulations to study disc formation in z ∼ 0, Milky Way-mass galaxies, and conclude that a key ingredient for the formation of thin stellar discs is the ability for accreting gas to develop an aligned angular momentum distribution via internal cancellation prior to joining the galaxy. Among galaxies with a high fraction (⁠>70 per cent⁠) of their young stars in a thin disc (h/R ∼ 0.1), we find that: (i) hot, virial-temperature gas dominates the inflowing gas mass on halo scales (≳20 kpc), with radiative losses offset by compression heating; (ii) this hot accretion proceeds until angular momentum support slows inward motion, at which point the gas cools to ≲104K⁠; (iii) prior to cooling, the accreting gas develops an angular momentum distribution that is aligned with the galaxy disc, and while cooling transitions from a quasi-spherical spatial configuration to a more-flattened, disc-like configuration. We show that the existence of this ‘rotating cooling flow’ accretion mode is strongly correlated with the fraction of stars forming in a thin disc, using a sample of 17 z ∼ 0 galaxies spanning a halo mass range of 1010.5 M⊙ ≲ Mh ≲ 1012 M⊙ and stellar mass range of 108 M⊙ ≲ M⋆ ≲ 1011 M⊙. Notably, galaxies with a thick disc or irregular morphology do not undergo significant angular momentum alignment of gas prior to accretion and show no correspondence between halo gas cooling and flattening. Our results suggest that rotating cooling flows (or, more generally, rotating subsonic flows) that become coherent and angular momentum-supported prior to accretion on to the galaxy are likely a necessary condition for the formation of thin, star-forming disc galaxies in a ΛCDM universe

    What Causes The Formation of Disks and End of Bursty Star Formation?

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    As they grow, galaxies can transition from irregular/spheroidal with 'bursty' star formation histories (SFHs), to disky with smooth SFHs. But even in simulations, the direct physical cause of such transitions remains unclear. We therefore explore this in a large suite of numerical experiments re-running portions of cosmological simulations with widely varied physics, further validated with existing FIRE simulations. We show that gas supply, cooling/thermodynamics, star formation model, Toomre scale, galaxy dynamical times, and feedback properties do not have a direct causal effect on these transitions. Rather, both the formation of disks and cessation of bursty star formation are driven by the gravitational potential, but in different ways. Disk formation is promoted when the mass profile becomes sufficiently centrally-concentrated in shape (relative to circularization radii): we show that this provides a well-defined dynamical center, ceases to support the global 'breathing modes' which can persist indefinitely in less-concentrated profiles and efficiently destroy disks, promotes orbit mixing to form a coherent angular momentum, and stabilizes the disk. Smooth SF is promoted by the potential or escape velocity (not circular velocity) becoming sufficiently large at the radii of star formation that cool, mass-loaded (momentum-conserving) outflows are trapped/confined near the galaxy, as opposed to escaping after bursts. We discuss the detailed physics, how these conditions arise in cosmological contexts, their relation to other correlated phenomena (e.g. inner halo virialization, vertical disk 'settling'), and observations.Comment: Submitted to MNRAS. 44 pages, 32 figures. Comments welcome. (Minor text corrections from previous version

    SRGeJ045359.9+622444: A 55-min Period Eclipsing AM CVn Discovered from a Joint SRG/eROSITA + ZTF Search

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    AM CVn systems are ultra-compact binaries where a white dwarf accretes from a helium-rich degenerate or semi-degenerate donor. Some AM CVn systems will be among the loudest sources of gravitational waves for the upcoming Laser Interferometer Space Antenna (LISA), yet the formation channel of AM CVns remains uncertain. We report the study and characterisation of a new eclipsing AM CVn, SRGeJ045359.9+622444 (hereafter SRGeJ0453), discovered from a joint SRG/eROSITA and ZTF program to identify cataclysmic variables (CVs). We obtained optical photometry to confirm the eclipse of SRGeJ0453 and determine the orbital period to be Porb=55.0802±0.0003P_\textrm{orb} = 55.0802 \pm 0.0003 min. We constrain the binary parameters by modeling the high-speed photometry and radial velocity curves and find Mdonor=0.044±0.024MM_\textrm{donor} = 0.044 \pm0.024 M_{\odot} and Rdonor=0.078±0.012RR_\textrm{donor}=0.078 \pm 0.012 R_{\odot}. The X-ray spectrum is approximated by a power-law model with an unusually flat photon index of Γ1\Gamma\sim 1 previously seen in magnetic CVs with SRG/eROSITA, but verifying the magnetic nature of SRGeJ0453 requires further investigation. Optical spectroscopy suggests that the donor star of SRGeJ0453 could have initially been a He star or a He white dwarf. SRGeJ0453 is the ninth eclipsing AM CVn system published to date, and its lack of optical outbursts have made it elusive in previous surveys. The discovery of SRGeJ0453 using joint X-ray and optical surveys highlights the potential for discovering similar systems in the near future.Comment: Submitted to Ap

    Making use of apex predator sample collections: an integrated workflow for quality assured sample processing, analysis and digital sample freezing of archived samples

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    Using monitoring data from apex predators for chemicals risk assessment can provide important information on bioaccumulating as well as biomagnifying chemicals in food webs. A survey among European institutions involved in chemical risk assessment on their experiences with apex predator data in chemical risk assessment revealed great interest in using such data. However, the respondents indicated that constraints were related to expected high costs, lack of standardisation and harmonised quality criteria for exposure assessment, data access, and regulatory acceptance/application. During the Life APEX project, we demonstrated that European sample collections (i.e. environmental specimen banks (ESBs), research collection (RCs), natural history museums (NHMs)) archive a large variety of biological samples that can be readily used for chemical analysis once appropriate quality assurance/control (QA/QC) measures have been developed and implemented. We therefore issued a second survey on sampling, processing and archiving procedures in European sample collections to derive key quality QA/QC criteria for chemical analysis. The survey revealed great differences in QA/QC measures between ESBs, NHMs and RCs. Whereas basic information such as sampling location, date and biometric data were mostly available across institutions, protocols to accompany the sampling strategy with respect to chemical analysis were only available for ESBs. For RCs, the applied QA/QC measures vary with the respective research question, whereas NHMs are generally less aware of e.g. chemical cross-contamination issues. Based on the survey we derived key indicators for assessing the quality of biota samples that can be easily implemented in online databases. Furthermore, we provide a QA/QC workflow not only for sampling and processing but also for the chemical analysis of biota samples. We focussed on comprehensive analytical techniques such as non-target screening and provided insights into subsequent storage of high-resolution chromatograms in online databases (i.e. digital sample freezing platform) to ultimately support chemicals risk assessment

    The importance of in-year seasonal fluctuations for biomonitoring of apex predators: a case study of 14 essential and non-essential elements in the liver of the common buzzard (Buteo buteo) in the United Kingdom

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    Trace elements are chemical contaminants spread in the environment by anthropogenic activities and threaten wildlife and human health. Many studies have investigated this contamination in apex raptors as sentinel birds. However, there is limited data for long-term biomonitoring of multiple trace elements in raptors. In the present study, we measured the concentrations of 14 essential and non-essential trace elements in the livers of the common buzzard (Buteo buteo) collected in the United Kingdom from 2001 to 2019 and investigated whether concentrations have changed during this period. In addition, we estimated the importance of selected variables for modelling element accumulations in tissues. •Except for cadmium, hepatic concentrations of harmful elements in most buzzards were lower than the biological significance level of each element. Hepatic concentrations of certain elements, including lead, cadmium, and arsenic, varied markedly seasonally within years. Their peak was in late winter and trough in late summer, except copper which showed an opposite seasonal pattern. In addition, lead in the liver consistently increased over time, whereas strontium showed a decreasing trend. Hepatic concentrations of cadmium, mercury, and chromium increased with age, whereas selenium and chromium were influenced by sex. Hepatic concentrations of arsenic and chromium also differed between different regions. •Overall, our samples showed a low risk of harmful effects of most elements compared to the thresholds reported in the literature. Seasonal fluctuation was an important descriptor of exposure, which might be related to the diet of the buzzard, the ecology of their prey, and human activities such as the use of lead shot for hunting. However, elucidating reasons for these observed trends needs further examination, and biomonitoring studies exploring the effects of variables such as age, sex, and seasonality are required
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